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The map label for this gene is panB

Identifier: 78356545

GI number: 78356545

Start: 1519790

End: 1520659

Strand: Reverse

Name: panB

Synonym: Dde_1500

Alternate gene names: 78356545

Gene position: 1520659-1519790 (Counterclockwise)

Preceding gene: 78356557

Following gene: 78356543

Centisome position: 40.77

GC content: 63.33

Gene sequence:

>870_bases
ATGAGCACACATGCCGAAACCCGCGCCATAACCGCGGCGGACATACGGGCGGCAAAAAACACAAGACGTCTGGCCATGCT
GACGGCATACGACTACCCCACGGCATCCATTGCCGATGAAGGCGGCATGGACATGCTGCTGGTAGGCGATTCGCTGGCCA
TGGTGGTGCTGGGGCATGAGGACACGCTGTCCGTGACACTGGACGAGATGATCCACCACTGCCGGGCCGTCACGCGGGGC
GCCTCACGCGCGCTGGTGGTGGGCGACCTGCCCTTTATGACCTATGAACAGGGGCCGGATCAGGCCATGCACAGCGCGGC
GCGACTGTTCCGCGAAGGCGGTGTGCGCGCTGTAAAGCTGGAAGGGGGCAAAGAAGTCGCTCCGCAGGTGGAGGCTCTTG
TGAAGGCGGGGATTCCCGTCATGGGGCACATCGGGCTTACTCCGCAGCGTGTAGCGGCGCTGGGCGGCTTTAAAGTGCAG
GGGCGTTCGGCAGCCGCCGCCCGCAGTCTGGCGGAAGATGCGCGCATACTGGAAGATGCGGGCTGTTTTGCACTGGTACT
GGAAGCCATACCCGCCCCTGTGGCCGCGCACATTACCCGCACGTCAGGCATTCCCACCATCGGCATAGGGGCCGGAGCGC
AGTGCGACGGACAGGTGCTGGTGGTGCACGATATGCTGGGCCTGTTCGACCGCTTTACCCCGAAGTTTGTGAAACGCTAT
GCAGAGCTGCGCGGGCATGCCGTAAAAGCCGTGCAGCAGTATGGTGATGAAGTGCGTCAGGGAGAATTTCCCGCGGCGCA
GCACTCCTTCGGCATGCCGGAAGACGAACAGCGCCGCTGGGAAGAAAATGTCAGCGGCGCGGACGACTGA

Upstream 100 bases:

>100_bases
AAGCATAAATCGTGCCAGATGCCCGCAAGTGCAGCACCGAAAGGGATTGGCGACAGACACGCTTTGCCTTATGGTACAGA
CAAAACACGGAGGAACCGTA

Downstream 100 bases:

>100_bases
TGCTCATCCCCCGAGGCGGCGGAGCATCCAGTCCCTGCAGCAGCAGGCAGGACGAGGGAATAATCCGCACAAAGCGTTCC
TCGTTCACACGTTCACGAAA

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MSTHAETRAITAADIRAAKNTRRLAMLTAYDYPTASIADEGGMDMLLVGDSLAMVVLGHEDTLSVTLDEMIHHCRAVTRG
ASRALVVGDLPFMTYEQGPDQAMHSAARLFREGGVRAVKLEGGKEVAPQVEALVKAGIPVMGHIGLTPQRVAALGGFKVQ
GRSAAAARSLAEDARILEDAGCFALVLEAIPAPVAAHITRTSGIPTIGIGAGAQCDGQVLVVHDMLGLFDRFTPKFVKRY
AELRGHAVKAVQQYGDEVRQGEFPAAQHSFGMPEDEQRRWEENVSGADD

Sequences:

>Translated_289_residues
MSTHAETRAITAADIRAAKNTRRLAMLTAYDYPTASIADEGGMDMLLVGDSLAMVVLGHEDTLSVTLDEMIHHCRAVTRG
ASRALVVGDLPFMTYEQGPDQAMHSAARLFREGGVRAVKLEGGKEVAPQVEALVKAGIPVMGHIGLTPQRVAALGGFKVQ
GRSAAAARSLAEDARILEDAGCFALVLEAIPAPVAAHITRTSGIPTIGIGAGAQCDGQVLVVHDMLGLFDRFTPKFVKRY
AELRGHAVKAVQQYGDEVRQGEFPAAQHSFGMPEDEQRRWEENVSGADD
>Mature_288_residues
STHAETRAITAADIRAAKNTRRLAMLTAYDYPTASIADEGGMDMLLVGDSLAMVVLGHEDTLSVTLDEMIHHCRAVTRGA
SRALVVGDLPFMTYEQGPDQAMHSAARLFREGGVRAVKLEGGKEVAPQVEALVKAGIPVMGHIGLTPQRVAALGGFKVQG
RSAAAARSLAEDARILEDAGCFALVLEAIPAPVAAHITRTSGIPTIGIGAGAQCDGQVLVVHDMLGLFDRFTPKFVKRYA
ELRGHAVKAVQQYGDEVRQGEFPAAQHSFGMPEDEQRRWEENVSGADD

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=255, Percent_Identity=45.0980392156863, Blast_Score=215, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6319653, Length=288, Percent_Identity=39.2361111111111, Blast_Score=202, Evalue=4e-53,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_DESDG (Q311U7)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_387994.1
- ProteinModelPortal:   Q311U7
- SMR:   Q311U7
- STRING:   Q311U7
- GeneID:   3756053
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_1500
- NMPDR:   fig|207559.3.peg.707
- eggNOG:   COG0413
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- ProtClustDB:   PRK00311
- BioCyc:   DDES207559:DDE_1500-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 30855; Mature: 30723

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: ACT_SITE 188-188 BINDING 89-89 BINDING 119-119

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTHAETRAITAADIRAAKNTRRLAMLTAYDYPTASIADEGGMDMLLVGDSLAMVVLGHE
CCCCCHHHEEHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCEEEEECCCEEEEEECCC
DTLSVTLDEMIHHCRAVTRGASRALVVGDLPFMTYEQGPDQAMHSAARLFREGGVRAVKL
CCCEEEHHHHHHHHHHHHCCCCCEEEEECCCCEECCCCCCHHHHHHHHHHHHCCCEEEEE
EGGKEVAPQVEALVKAGIPVMGHIGLTPQRVAALGGFKVQGRSAAAARSLAEDARILEDA
CCCCCHHHHHHHHHHCCCCEEECCCCCHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHC
GCFALVLEAIPAPVAAHITRTSGIPTIGIGAGAQCDGQVLVVHDMLGLFDRFTPKFVKRY
CHHHHHHHHHCCCHHHHHHHCCCCCEEECCCCCCCCCCEEEHHHHHHHHHHCCHHHHHHH
AELRGHAVKAVQQYGDEVRQGEFPAAQHSFGMPEDEQRRWEENVSGADD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure 
STHAETRAITAADIRAAKNTRRLAMLTAYDYPTASIADEGGMDMLLVGDSLAMVVLGHE
CCCCHHHEEHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCEEEEECCCEEEEEECCC
DTLSVTLDEMIHHCRAVTRGASRALVVGDLPFMTYEQGPDQAMHSAARLFREGGVRAVKL
CCCEEEHHHHHHHHHHHHCCCCCEEEEECCCCEECCCCCCHHHHHHHHHHHHCCCEEEEE
EGGKEVAPQVEALVKAGIPVMGHIGLTPQRVAALGGFKVQGRSAAAARSLAEDARILEDA
CCCCCHHHHHHHHHHCCCCEEECCCCCHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHC
GCFALVLEAIPAPVAAHITRTSGIPTIGIGAGAQCDGQVLVVHDMLGLFDRFTPKFVKRY
CHHHHHHHHHCCCHHHHHHHCCCCCEEECCCCCCCCCCEEEHHHHHHHHHHCCHHHHHHH
AELRGHAVKAVQQYGDEVRQGEFPAAQHSFGMPEDEQRRWEENVSGADD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA