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The map label for this gene is trmH [H]

Identifier: 78356529

GI number: 78356529

Start: 1504239

End: 1504829

Strand: Direct

Name: trmH [H]

Synonym: Dde_1484

Alternate gene names: 78356529

Gene position: 1504239-1504829 (Clockwise)

Preceding gene: 78356527

Following gene: 78356532

Centisome position: 40.33

GC content: 55.5

Gene sequence:

>591_bases
ATGACCAGAGAACGTACTTCAGAAAGGGTGGAGAAAATCAAGAGGGTGCTGTCGCTGAGGCAGAAAGACCTTACTCTTGT
GCTTGCTAATATACACGATCCGCACAATGTGTCGGCCATCTACCGCAGCTGCGACGCTTTCGGCGTTTCCAGAGTCCATC
TGTACTATACGGATACCCCTTTTCCCCCGCTCAGCGGACGCTCTTCGGCATCTGCCCGCAAATGGGTGGAGACTGAAAGG
CACGAAGATCCCGCACAGCTGGCCGCTGCGCTCAGAAAAAAAGGATGCCGTATTCTTGCCACCAGCTGTTCGCCCTCGGC
CCGGCCGCTGCGCGAATGGGACATGACCGGTCCCACGGCGATCATTATGGGCAACGAACACCGCGGGGTGGACGCCGAGC
TTATGGAAATAGTGGACGGTGAAGTCTATATCCCCATGTTCGGCATGATTCAGAGCTTCAATGTCTCGGTGGCTGCGGCT
GTTATTTTGTCCGAGGCTTCGCGGCAGCGTCAGCAGGCGGGTAAATACGAGACTCCCACATATACCGAAGAAGAATACGC
TGCGGTGCTGGAAGAGTGGCTGGCAAAATAA

Upstream 100 bases:

>100_bases
CCGCAGGCACGGGGTGACAGCAGGCCCGTTCCGCTGTATGTAACGCTGGATTTATGCACGGAACCGAAAAGTCAGAACGA
TATGAACGGATGAAAATGAT

Downstream 100 bases:

>100_bases
GTTGTCAGCTTTCTGCTGGTTGTGCTGCGTATAGACACGGCCCGTGTGTGGGGGCGGACTCTGACGGACGCAATCAGCCG
CCGCGTGACATGCAGGTTCA

Product: tRNA (guanosine-2'-O-)-methyltransferase

Products: NA

Alternate protein names: tRNA [Gm18] methyltransferase [H]

Number of amino acids: Translated: 196; Mature: 195

Protein sequence:

>196_residues
MTRERTSERVEKIKRVLSLRQKDLTLVLANIHDPHNVSAIYRSCDAFGVSRVHLYYTDTPFPPLSGRSSASARKWVETER
HEDPAQLAAALRKKGCRILATSCSPSARPLREWDMTGPTAIIMGNEHRGVDAELMEIVDGEVYIPMFGMIQSFNVSVAAA
VILSEASRQRQQAGKYETPTYTEEEYAAVLEEWLAK

Sequences:

>Translated_196_residues
MTRERTSERVEKIKRVLSLRQKDLTLVLANIHDPHNVSAIYRSCDAFGVSRVHLYYTDTPFPPLSGRSSASARKWVETER
HEDPAQLAAALRKKGCRILATSCSPSARPLREWDMTGPTAIIMGNEHRGVDAELMEIVDGEVYIPMFGMIQSFNVSVAAA
VILSEASRQRQQAGKYETPTYTEEEYAAVLEEWLAK
>Mature_195_residues
TRERTSERVEKIKRVLSLRQKDLTLVLANIHDPHNVSAIYRSCDAFGVSRVHLYYTDTPFPPLSGRSSASARKWVETERH
EDPAQLAAALRKKGCRILATSCSPSARPLREWDMTGPTAIIMGNEHRGVDAELMEIVDGEVYIPMFGMIQSFNVSVAAAV
ILSEASRQRQQAGKYETPTYTEEEYAAVLEEWLAK

Specific function: Specifically methylates guanosine-18 in various tRNAs [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=168, Percent_Identity=43.452380952381, Blast_Score=142, Evalue=1e-35,
Organism=Escherichia coli, GI1790623, Length=146, Percent_Identity=32.8767123287671, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022724
- InterPro:   IPR001537 [H]

Pfam domain/function: PF12105 SpoU_methylas_C; PF00588 SpoU_methylase [H]

EC number: =2.1.1.34 [H]

Molecular weight: Translated: 22041; Mature: 21910

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRERTSERVEKIKRVLSLRQKDLTLVLANIHDPHNVSAIYRSCDAFGVSRVHLYYTDTP
CCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEECCCC
FPPLSGRSSASARKWVETERHEDPAQLAAALRKKGCRILATSCSPSARPLREWDMTGPTA
CCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHCCCCCCEE
IIMGNEHRGVDAELMEIVDGEVYIPMFGMIQSFNVSVAAAVILSEASRQRQQAGKYETPT
EEECCCCCCCCHHHHHHHCCCEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCC
YTEEEYAAVLEEWLAK
CCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TRERTSERVEKIKRVLSLRQKDLTLVLANIHDPHNVSAIYRSCDAFGVSRVHLYYTDTP
CCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEECCCC
FPPLSGRSSASARKWVETERHEDPAQLAAALRKKGCRILATSCSPSARPLREWDMTGPTA
CCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHCCCCCCEE
IIMGNEHRGVDAELMEIVDGEVYIPMFGMIQSFNVSVAAAVILSEASRQRQQAGKYETPT
EEECCCCCCCCHHHHHHHCCCEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCC
YTEEEYAAVLEEWLAK
CCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]