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The map label for this gene is rfaQ [H]

Identifier: 78356527

GI number: 78356527

Start: 1500816

End: 1501850

Strand: Direct

Name: rfaQ [H]

Synonym: Dde_1482

Alternate gene names: 78356527

Gene position: 1500816-1501850 (Clockwise)

Preceding gene: 78356526

Following gene: 78356529

Centisome position: 40.23

GC content: 60.39

Gene sequence:

>1035_bases
ATGCGACGCATAGCCGTCTGGAACACCGCTTTTCTCGGCGATGCCGTGCTGACGCTGCCGCTTATCAGCACGCTGAAGGC
TGCCTATCCTGATGCCGCCATTGATTTCTGGGTGCGCAAGGGGTTTGAGGGGCTGTTCGCATCCCAGCCGGAGCTGGCGG
GGGTGTACGGGTATGACAAGAATGGAGCACAGGGCGGTCCGGGCGGAACTTTCGCTCTGGGCAGGCATATGGCGGCACAG
GGGTATTCGCTTTTTGTATCGGCCCATACCAGTGTCCGCAGCGGCCTGATGGCCCGCTGGTCAAGCGCCAAGACGCGTAT
CGGGTATGACAGACCTTTTCTGAACAGATATTTTTATACGCATACCGTTCCGCGCTGCTTCGACTCCATGGAAGAAATCG
AACGCCTGCTGCAACTGGTAAAGCCACTGGGAATCAGCGAACTGCACACATGGCCCCGGCTGGTGCTGCCGCAGGATGCC
CGCCATAAAGCCGAAGGATATTTTGCCAGCCATGTGAAAGGTCCGGTTGTGGGGCTGCATCCCGGTTCGGTATGGGGAAC
AAAACGATGGCCTGCCGCCTCGTATGCGCAGGTTGCTTCGCACGCAGTTGCCGCGGGGGCGCAGGTGATGATTTTTGCCG
GTCCCGGAGAAGAAGCCATGGCGCAGGATGTGATCAGCCGCATTGATACCGGGGGCAGACGCCACGCTGTGCTTGATCTT
TCGGGCAGTCTTTCGCTTCCCGAGCTTGCGGCCTTTCTGGGACGTCTGGACTGTTATGTGACCAATGATTCAGGCCCCAT
GCATCTTGCATGGGTTCAGCATACTCCGGTGACGGCCATTTTCGGCCCTACCGTCCGGCGTCTGGGTTTTTTCCCCCGCG
GCAGCCGGTCCACGGTGCTGGAGGCCGATGTCTCCTGTCGTCCGTGCGGCCTGCATGGCCCGCAGGAATGCCCGCAGGGG
CACCACCGCTGTATGGCTGAGGTGACTCCCGGCATGGTCTGGGCGGATGTGGAGCGCAAGCTTTTCCGCACATGA

Upstream 100 bases:

>100_bases
GGGTTGAAGTGCATAAACGCTCAGGCGTGCTGCCGGATGTTGTGGCATGCGATGTGCGTGCCGCGCTCGACTGGGTAAGA
ACTGTGCTGGAGGAGAAATA

Downstream 100 bases:

>100_bases
TGCTTTTGGATGTGGACATGATGACGGCGACGGCGCCCTGCGGTTACGCGGGGCGTTTTTTTGTGTTTTCTGCCCGTGCT
GCGCGGAACGTGATGCGCGG

Product: heptosyltransferase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MRRIAVWNTAFLGDAVLTLPLISTLKAAYPDAAIDFWVRKGFEGLFASQPELAGVYGYDKNGAQGGPGGTFALGRHMAAQ
GYSLFVSAHTSVRSGLMARWSSAKTRIGYDRPFLNRYFYTHTVPRCFDSMEEIERLLQLVKPLGISELHTWPRLVLPQDA
RHKAEGYFASHVKGPVVGLHPGSVWGTKRWPAASYAQVASHAVAAGAQVMIFAGPGEEAMAQDVISRIDTGGRRHAVLDL
SGSLSLPELAAFLGRLDCYVTNDSGPMHLAWVQHTPVTAIFGPTVRRLGFFPRGSRSTVLEADVSCRPCGLHGPQECPQG
HHRCMAEVTPGMVWADVERKLFRT

Sequences:

>Translated_344_residues
MRRIAVWNTAFLGDAVLTLPLISTLKAAYPDAAIDFWVRKGFEGLFASQPELAGVYGYDKNGAQGGPGGTFALGRHMAAQ
GYSLFVSAHTSVRSGLMARWSSAKTRIGYDRPFLNRYFYTHTVPRCFDSMEEIERLLQLVKPLGISELHTWPRLVLPQDA
RHKAEGYFASHVKGPVVGLHPGSVWGTKRWPAASYAQVASHAVAAGAQVMIFAGPGEEAMAQDVISRIDTGGRRHAVLDL
SGSLSLPELAAFLGRLDCYVTNDSGPMHLAWVQHTPVTAIFGPTVRRLGFFPRGSRSTVLEADVSCRPCGLHGPQECPQG
HHRCMAEVTPGMVWADVERKLFRT
>Mature_344_residues
MRRIAVWNTAFLGDAVLTLPLISTLKAAYPDAAIDFWVRKGFEGLFASQPELAGVYGYDKNGAQGGPGGTFALGRHMAAQ
GYSLFVSAHTSVRSGLMARWSSAKTRIGYDRPFLNRYFYTHTVPRCFDSMEEIERLLQLVKPLGISELHTWPRLVLPQDA
RHKAEGYFASHVKGPVVGLHPGSVWGTKRWPAASYAQVASHAVAAGAQVMIFAGPGEEAMAQDVISRIDTGGRRHAVLDL
SGSLSLPELAAFLGRLDCYVTNDSGPMHLAWVQHTPVTAIFGPTVRRLGFFPRGSRSTVLEADVSCRPCGLHGPQECPQG
HHRCMAEVTPGMVWADVERKLFRT

Specific function: Catalyzes heptose transfer to the lipopolysaccharide core. It transfers a heptose, called heptose(III), to the heptose(II) of the inner core [H]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790062, Length=286, Percent_Identity=27.972027972028, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790050, Length=354, Percent_Identity=24.0112994350282, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011916 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 37599; Mature: 37599

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRIAVWNTAFLGDAVLTLPLISTLKAAYPDAAIDFWVRKGFEGLFASQPELAGVYGYDK
CCEEEEECHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHCCCCCCCEECCCCC
NGAQGGPGGTFALGRHMAAQGYSLFVSAHTSVRSGLMARWSSAKTRIGYDRPFLNRYFYT
CCCCCCCCCHHHHHHHHHHCCEEEEEEECHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
HTVPRCFDSMEEIERLLQLVKPLGISELHTWPRLVLPQDARHKAEGYFASHVKGPVVGLH
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEECCCCHHHHHCCHHHHCCCCCEEEEC
PGSVWGTKRWPAASYAQVASHAVAAGAQVMIFAGPGEEAMAQDVISRIDTGGRRHAVLDL
CCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEE
SGSLSLPELAAFLGRLDCYVTNDSGPMHLAWVQHTPVTAIFGPTVRRLGFFPRGSRSTVL
CCCCCHHHHHHHHHCCEEEEECCCCCEEEEEEECCCEEEECCHHHHHHCCCCCCCCCEEE
EADVSCRPCGLHGPQECPQGHHRCMAEVTPGMVWADVERKLFRT
EECCCCCCCCCCCCHHCCCHHHHHHHHCCCCEEEHHHHHHHHCC
>Mature Secondary Structure
MRRIAVWNTAFLGDAVLTLPLISTLKAAYPDAAIDFWVRKGFEGLFASQPELAGVYGYDK
CCEEEEECHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHCCCCCCCEECCCCC
NGAQGGPGGTFALGRHMAAQGYSLFVSAHTSVRSGLMARWSSAKTRIGYDRPFLNRYFYT
CCCCCCCCCHHHHHHHHHHCCEEEEEEECHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
HTVPRCFDSMEEIERLLQLVKPLGISELHTWPRLVLPQDARHKAEGYFASHVKGPVVGLH
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEECCCCHHHHHCCHHHHCCCCCEEEEC
PGSVWGTKRWPAASYAQVASHAVAAGAQVMIFAGPGEEAMAQDVISRIDTGGRRHAVLDL
CCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEE
SGSLSLPELAAFLGRLDCYVTNDSGPMHLAWVQHTPVTAIFGPTVRRLGFFPRGSRSTVL
CCCCCHHHHHHHHHCCEEEEECCCCCEEEEEEECCCEEEECCHHHHHHCCCCCCCCCEEE
EADVSCRPCGLHGPQECPQGHHRCMAEVTPGMVWADVERKLFRT
EECCCCCCCCCCCCHHCCCHHHHHHHHCCCCEEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Glycosyltransferases; Transferring other glycosyl groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1732225; 1447141; 8041620; 9278503 [H]