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The map label for this gene is hisA

Identifier: 78356510

GI number: 78356510

Start: 1486460

End: 1487197

Strand: Reverse

Name: hisA

Synonym: Dde_1465

Alternate gene names: 78356510

Gene position: 1487197-1486460 (Counterclockwise)

Preceding gene: 78356511

Following gene: 78356509

Centisome position: 39.87

GC content: 60.3

Gene sequence:

>738_bases
GTGATCATCTTTCCTGCTGTGGACATAAAGGGCGGGCAGGCGGTTCGCCTGCGGCAGGGCAAGGCCGATCAGGAAACGGT
TTTTTCCGGCGATCCTGTGGCCATGGCACGCCACTGGGAAGAACAGGGCGGACGCTGGCTGCATGTGATAGATCTGGACG
GTGCGTTTTCCGGCACTCCGGTCAACCGCGAGCTGATCAGAACCATCTGCGCCAGTGTATCCATTCCCGTCCAGCTGGGC
GGCGGCATACGCGATACCGTCACCGCGCAGGCGTATCTGGATGCCGGAGTTGAACGGCTGATAATCGGCACCATCGCCCT
GACCGATCCCGATCTGTTCGGGGCCATGTGTTCAGCGTTTCCGGGCCGTATAGGCGTTTCGCTGGACGCGGAAGGCGGCA
GACTGAAAACCAAAGGCTGGGTGGAAGATTCCGGCCTGAGCATTTACGACGTACTGCCCCGGCTGACAGAACAGGGAGCC
GCGTTCCTCATCTACACCGACATCGACCGCGACGGGATGCAGAGCGGTGTAAACGTGGAAGCTCTTTCCGAACTTGCCGT
CCGCTCGGCAATACCAGTCATAGCGGCAGGCGGCGTAGCGACCATGCAGGATGTCTACGATCTGTATCCTGTAAGCCGCA
GTGCCAACCTGCAGGGCGCCATTACCGGCAAGGCCATTTACGAGGGTTCGCTGGATCTGGCAGAAGCCATGCGCTGGATA
GACAGCCGGACAGCATGA

Upstream 100 bases:

>100_bases
TGCTGGAGTTCAGCAAATTCGTCGAACGCAAAGGCAAGTGGATCACAGCAGAAGATCTGGCCCGCGAAGGACTGTATCAG
GGAATCAAGGAGCTTGGTCT

Downstream 100 bases:

>100_bases
CGCATGCGCCATGCGTTGGCATGGCTGCAAGGCTTGCACCGGATTATGTACCGTCGTATTCAGGCACCGGAGCCGGTAGC
CGCAGACAGTATCATATGTT

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MIIFPAVDIKGGQAVRLRQGKADQETVFSGDPVAMARHWEEQGGRWLHVIDLDGAFSGTPVNRELIRTICASVSIPVQLG
GGIRDTVTAQAYLDAGVERLIIGTIALTDPDLFGAMCSAFPGRIGVSLDAEGGRLKTKGWVEDSGLSIYDVLPRLTEQGA
AFLIYTDIDRDGMQSGVNVEALSELAVRSAIPVIAAGGVATMQDVYDLYPVSRSANLQGAITGKAIYEGSLDLAEAMRWI
DSRTA

Sequences:

>Translated_245_residues
MIIFPAVDIKGGQAVRLRQGKADQETVFSGDPVAMARHWEEQGGRWLHVIDLDGAFSGTPVNRELIRTICASVSIPVQLG
GGIRDTVTAQAYLDAGVERLIIGTIALTDPDLFGAMCSAFPGRIGVSLDAEGGRLKTKGWVEDSGLSIYDVLPRLTEQGA
AFLIYTDIDRDGMQSGVNVEALSELAVRSAIPVIAAGGVATMQDVYDLYPVSRSANLQGAITGKAIYEGSLDLAEAMRWI
DSRTA
>Mature_245_residues
MIIFPAVDIKGGQAVRLRQGKADQETVFSGDPVAMARHWEEQGGRWLHVIDLDGAFSGTPVNRELIRTICASVSIPVQLG
GGIRDTVTAQAYLDAGVERLIIGTIALTDPDLFGAMCSAFPGRIGVSLDAEGGRLKTKGWVEDSGLSIYDVLPRLTEQGA
AFLIYTDIDRDGMQSGVNVEALSELAVRSAIPVIAAGGVATMQDVYDLYPVSRSANLQGAITGKAIYEGSLDLAEAMRWI
DSRTA

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=245, Percent_Identity=32.6530612244898, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1788336, Length=246, Percent_Identity=24.7967479674797, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_DESDG (Q311Y2)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_387959.1
- ProteinModelPortal:   Q311Y2
- SMR:   Q311Y2
- STRING:   Q311Y2
- GeneID:   3756096
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_1465
- NMPDR:   fig|207559.3.peg.672
- eggNOG:   COG0106
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- ProtClustDB:   PRK00748
- BioCyc:   DDES207559:DDE_1465-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 26097; Mature: 26097

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIFPAVDIKGGQAVRLRQGKADQETVFSGDPVAMARHWEEQGGRWLHVIDLDGAFSGTP
CEEEEEEECCCCCEEEEECCCCCCCEEECCCCHHHHHHHHHCCCCEEEEEEECCCCCCCC
VNRELIRTICASVSIPVQLGGGIRDTVTAQAYLDAGVERLIIGTIALTDPDLFGAMCSAF
CCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCHHEEEEEEEEECCCHHHHHHHHCC
PGRIGVSLDAEGGRLKTKGWVEDSGLSIYDVLPRLTEQGAAFLIYTDIDRDGMQSGVNVE
CCCEEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCHHHHCCCCHH
ALSELAVRSAIPVIAAGGVATMQDVYDLYPVSRSANLQGAITGKAIYEGSLDLAEAMRWI
HHHHHHHHHCCCEEECCCHHHHHHHHHHCCCCCCCCCCEEEECCEEECCCCCHHHHHHHH
DSRTA
HCCCC
>Mature Secondary Structure
MIIFPAVDIKGGQAVRLRQGKADQETVFSGDPVAMARHWEEQGGRWLHVIDLDGAFSGTP
CEEEEEEECCCCCEEEEECCCCCCCEEECCCCHHHHHHHHHCCCCEEEEEEECCCCCCCC
VNRELIRTICASVSIPVQLGGGIRDTVTAQAYLDAGVERLIIGTIALTDPDLFGAMCSAF
CCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCHHEEEEEEEEECCCHHHHHHHHCC
PGRIGVSLDAEGGRLKTKGWVEDSGLSIYDVLPRLTEQGAAFLIYTDIDRDGMQSGVNVE
CCCEEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCHHHHCCCCHH
ALSELAVRSAIPVIAAGGVATMQDVYDLYPVSRSANLQGAITGKAIYEGSLDLAEAMRWI
HHHHHHHHHCCCEEECCCHHHHHHHHHHCCCCCCCCCCEEEECCEEECCCCCHHHHHHHH
DSRTA
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA