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The map label for this gene is hyfR [H]

Identifier: 78356444

GI number: 78356444

Start: 1414035

End: 1415294

Strand: Reverse

Name: hyfR [H]

Synonym: Dde_1399

Alternate gene names: 78356444

Gene position: 1415294-1414035 (Counterclockwise)

Preceding gene: 78356446

Following gene: 78356443

Centisome position: 37.94

GC content: 55.95

Gene sequence:

>1260_bases
ATGCATTATCTGAAAGACATCATGAACACACATTCGCCCACCACCATCAACGATCTTGATATTTCGCCGTTTTTCGACCT
GATGCTTGCCATGGCGCGCGAGCGTTCTGTCGAGGCGTTACTGGATCTTACCGAAAGAGCATACCGCGGGACCCATGTCA
TCAGCGGTGCCCTGTGGTTTGCAGAAAACAGTCCGGAAAGTCCGCGCGGCAAAATGCTGCGCCTTATGGCTCACTCCGGC
TGGACACGCCACACACCGCGCGAATGGCGGCACGATGAGGGTACATACACGCTGGTGCCGCTCACCGAACCTGTCATAGG
CGAATGTGCCCGCACGGCAAAGCCCATGTTTGCCGAAGACAGCGCCCAGTGGGAGAGGCCTGCATGGGCAGAAAATGAGG
GAATTCACGCCTACGCGACCTATCCCCTCATTTTCAAAAGCGAAATGCTGGGCGTGATGGCTGTTTTCTACGACCGGCCC
ATGCAGGACAGATTGTCTGATGTCATGCGTATGCATCGGAAAATGCATACTATTTTTGCTGATTCTCTTGCAGCCGCACT
GGCAAACGCCCGAGCTTTTGAAGAAATTCAGCGCCTGCGCCGCGAACTGGAAATGGAAAACGAGTACCTGCGCCGCGCGG
TGAGCACCGCCAATAATCTGGGCGACGACATTGTGGGTGAAAGCCCTTCTCTTAAAAAAGTCATGGAACAGATAGAGGTT
GTGGCTCCCACAGATGCAACGGTGCTGCTGCTGGGCGAATCCGGCACCGGCAAAGAGCTGCTGGCGCAGGCCATTCACGC
CAACAGCCAGCGCAGGGACAAGCCGCTCATACGGGTCAACTGCTCCGCCATCCCCCGCGAACTTTTCGAAAGTGAATTCT
TCGGGCACGTCAAAGGATCATTCACAGGCGCGGTACGCGACCGTACCGGGCGTTTTCAGCTGGCGGACGGCGGTACGCTT
TTTCTGGACGAAGTCGGGGAAATTCCGCTTGAACTGCAGGGCAAGCTGCTGCGGGTGCTGCAGGAAGGAACATTCGAGCG
TGTGGGTGATGAGCAGAGCAGAAAAGTTGACGTACGCATTGTCGCCGCCACCAACAAGGACCTGCTTGAAGAAGTGCGCA
AGGGAACATTCCGGCAGGACCTCTATTTCAGACTCAGTGTTTTTCCCATCCACAACCCGCCGCTGCGCGAACGCCCCGAA
GACATTCCGCTGCTGGCAAGGCACTTTGATCCTGCTACAAAGTTGTGGACACAGAGTTAA

Upstream 100 bases:

>100_bases
TAAGTCAGCAAAAACATACCATTACAGAGCAGCACCGCTTTGAGTGTCGCAACGATATTTCGTTGACAACGACATTTCGT
TACGATATTTCGTTGTTTAC

Downstream 100 bases:

>100_bases
GCTACTATCCTGCCTACGAAGGAGATATGGATGAGTAGCCCAAAACGTCGCACTTATGATTCAGAATTCAAACAAAACGC
CGTGTTATTAACAGAAGACC

Product: ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MHYLKDIMNTHSPTTINDLDISPFFDLMLAMARERSVEALLDLTERAYRGTHVISGALWFAENSPESPRGKMLRLMAHSG
WTRHTPREWRHDEGTYTLVPLTEPVIGECARTAKPMFAEDSAQWERPAWAENEGIHAYATYPLIFKSEMLGVMAVFYDRP
MQDRLSDVMRMHRKMHTIFADSLAAALANARAFEEIQRLRRELEMENEYLRRAVSTANNLGDDIVGESPSLKKVMEQIEV
VAPTDATVLLLGESGTGKELLAQAIHANSQRRDKPLIRVNCSAIPRELFESEFFGHVKGSFTGAVRDRTGRFQLADGGTL
FLDEVGEIPLELQGKLLRVLQEGTFERVGDEQSRKVDVRIVAATNKDLLEEVRKGTFRQDLYFRLSVFPIHNPPLRERPE
DIPLLARHFDPATKLWTQS

Sequences:

>Translated_419_residues
MHYLKDIMNTHSPTTINDLDISPFFDLMLAMARERSVEALLDLTERAYRGTHVISGALWFAENSPESPRGKMLRLMAHSG
WTRHTPREWRHDEGTYTLVPLTEPVIGECARTAKPMFAEDSAQWERPAWAENEGIHAYATYPLIFKSEMLGVMAVFYDRP
MQDRLSDVMRMHRKMHTIFADSLAAALANARAFEEIQRLRRELEMENEYLRRAVSTANNLGDDIVGESPSLKKVMEQIEV
VAPTDATVLLLGESGTGKELLAQAIHANSQRRDKPLIRVNCSAIPRELFESEFFGHVKGSFTGAVRDRTGRFQLADGGTL
FLDEVGEIPLELQGKLLRVLQEGTFERVGDEQSRKVDVRIVAATNKDLLEEVRKGTFRQDLYFRLSVFPIHNPPLRERPE
DIPLLARHFDPATKLWTQS
>Mature_419_residues
MHYLKDIMNTHSPTTINDLDISPFFDLMLAMARERSVEALLDLTERAYRGTHVISGALWFAENSPESPRGKMLRLMAHSG
WTRHTPREWRHDEGTYTLVPLTEPVIGECARTAKPMFAEDSAQWERPAWAENEGIHAYATYPLIFKSEMLGVMAVFYDRP
MQDRLSDVMRMHRKMHTIFADSLAAALANARAFEEIQRLRRELEMENEYLRRAVSTANNLGDDIVGESPSLKKVMEQIEV
VAPTDATVLLLGESGTGKELLAQAIHANSQRRDKPLIRVNCSAIPRELFESEFFGHVKGSFTGAVRDRTGRFQLADGGTL
FLDEVGEIPLELQGKLLRVLQEGTFERVGDEQSRKVDVRIVAATNKDLLEEVRKGTFRQDLYFRLSVFPIHNPPLRERPE
DIPLLARHFDPATKLWTQS

Specific function: Required for induction of expression of the hydrogenase- 4 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=231, Percent_Identity=53.2467532467532, Blast_Score=261, Evalue=6e-71,
Organism=Escherichia coli, GI1789087, Length=294, Percent_Identity=46.2585034013605, Blast_Score=256, Evalue=3e-69,
Organism=Escherichia coli, GI87082152, Length=192, Percent_Identity=56.25, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1790437, Length=194, Percent_Identity=56.701030927835, Blast_Score=224, Evalue=9e-60,
Organism=Escherichia coli, GI1788550, Length=215, Percent_Identity=52.093023255814, Blast_Score=222, Evalue=4e-59,
Organism=Escherichia coli, GI1790299, Length=192, Percent_Identity=55.2083333333333, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI1788905, Length=186, Percent_Identity=53.2258064516129, Blast_Score=207, Evalue=9e-55,
Organism=Escherichia coli, GI1789233, Length=189, Percent_Identity=49.7354497354497, Blast_Score=198, Evalue=7e-52,
Organism=Escherichia coli, GI87081872, Length=188, Percent_Identity=50.531914893617, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1786524, Length=208, Percent_Identity=48.0769230769231, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1787583, Length=186, Percent_Identity=46.2365591397849, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI1789828, Length=191, Percent_Identity=39.2670157068063, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI87081858, Length=161, Percent_Identity=40.3726708074534, Blast_Score=115, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 47715; Mature: 47715

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHYLKDIMNTHSPTTINDLDISPFFDLMLAMARERSVEALLDLTERAYRGTHVISGALWF
CCHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
AENSPESPRGKMLRLMAHSGWTRHTPREWRHDEGTYTLVPLTEPVIGECARTAKPMFAED
ECCCCCCCHHHHHHHHHHCCCCCCCCHHHCCCCCCEEEEECCCHHHHHHHHHCCCCCCCC
SAQWERPAWAENEGIHAYATYPLIFKSEMLGVMAVFYDRPMQDRLSDVMRMHRKMHTIFA
CCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
DSLAAALANARAFEEIQRLRRELEMENEYLRRAVSTANNLGDDIVGESPSLKKVMEQIEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHE
VAPTDATVLLLGESGTGKELLAQAIHANSQRRDKPLIRVNCSAIPRELFESEFFGHVKGS
ECCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHCCCCCC
FTGAVRDRTGRFQLADGGTLFLDEVGEIPLELQGKLLRVLQEGTFERVGDEQSRKVDVRI
CCCCEECCCCEEEECCCCEEEECHHCCCCHHHHHHHHHHHHHCCHHHCCCCCCCEEEEEE
VAATNKDLLEEVRKGTFRQDLYFRLSVFPIHNPPLRERPEDIPLLARHFDPATKLWTQS
EEECCHHHHHHHHHCCHHHCEEEEEEEEECCCCCCCCCCCCCCHHHHHCCHHHHHCCCC
>Mature Secondary Structure
MHYLKDIMNTHSPTTINDLDISPFFDLMLAMARERSVEALLDLTERAYRGTHVISGALWF
CCHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
AENSPESPRGKMLRLMAHSGWTRHTPREWRHDEGTYTLVPLTEPVIGECARTAKPMFAED
ECCCCCCCHHHHHHHHHHCCCCCCCCHHHCCCCCCEEEEECCCHHHHHHHHHCCCCCCCC
SAQWERPAWAENEGIHAYATYPLIFKSEMLGVMAVFYDRPMQDRLSDVMRMHRKMHTIFA
CCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
DSLAAALANARAFEEIQRLRRELEMENEYLRRAVSTANNLGDDIVGESPSLKKVMEQIEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHE
VAPTDATVLLLGESGTGKELLAQAIHANSQRRDKPLIRVNCSAIPRELFESEFFGHVKGS
ECCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHCCCCCC
FTGAVRDRTGRFQLADGGTLFLDEVGEIPLELQGKLLRVLQEGTFERVGDEQSRKVDVRI
CCCCEECCCCEEEECCCCEEEECHHCCCCHHHHHHHHHHHHHCCHHHCCCCCCCEEEEEE
VAATNKDLLEEVRKGTFRQDLYFRLSVFPIHNPPLRERPEDIPLLARHFDPATKLWTQS
EEECCHHHHHHHHHCCHHHCEEEEEEEEECCCCCCCCCCCCCCHHHHHCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]