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The map label for this gene is fliY [H]

Identifier: 78356430

GI number: 78356430

Start: 1403381

End: 1404196

Strand: Direct

Name: fliY [H]

Synonym: Dde_1385

Alternate gene names: 78356430

Gene position: 1403381-1404196 (Clockwise)

Preceding gene: 78356429

Following gene: 78356431

Centisome position: 37.62

GC content: 51.72

Gene sequence:

>816_bases
ATGAAGTTTGTTAAGGCGCTTGTCCTGCTGCTGGTTACTGTTATGGCGGTACCGGCTTTTGCAGGCGACATCGATCTGGC
CAAAAAATCCGTTCTGAACGACATCCTGAAGCGCGGAGAACTGCGCGTCGGCTTCGACGCCGGTTATCCCCCCTTTGAGA
TGACGGACAAGTCCGGCAATTTTGTGGGCTTTGACATTGACCTCGGCAAAGAGCTTGCCAAATCCATGGGTGTTAAGTTC
GTTCCTGTGAATACCGATTTCGACGGTATGATTCCTTCGCTGCTTTCCGGAAAGTTTGACGTGATCATTTCCGGCATGAC
ACTGACGCAGGAACGCAACATGAAGATCGGTTTTTCCGAACCGTACCTTGTCATGGGGCAGACCGTGCTGGTAGCTGCAA
AGCATAAAGGCACCGTGAAATCCTACAAGGATCTCAATGATCCCAAGTTCATCGTTGTTTCCCGCATGGGTACCACCGGT
GAAGAAGCCACCAAAAAATACCTGCCCAAGGCGACCTACAAGTCGTTTGACAAAGAAGTGGACTGCGCCATGGAAGTGGT
GAACGGCCGTGCCGACGCGTTTGTGTACGACCTGCCGGTGAACGAGGTGTTTCAGGCACAGCAGGGCAAGGACAAAACCT
ACCTGCTGAACGAGCCTTTCACCTTTGAACCCATGGCTGTGGGCTACAAGCAGGGTGATCCTGACTTTGCAAACTTTATC
AATCATTTTCTGCGTCAGCTGAAAAACGACGGCCGCTACGAGCGCATGTACAACAAGTGGCTGCGTTCCGACGCATGGCA
GTCTCAGCTTAAATAA

Upstream 100 bases:

>100_bases
TGCCGCAGAGATATGATCATCAAGGAAGTGCTGACCGAGGGCAAATATTGATGAGGAGAGGCCCCGCGGGGTCTGACGGG
AAAATACTGGAGGTGTCCAA

Downstream 100 bases:

>100_bases
TCAGGGCGTAAGCCATATGGCGGGCCGGAACTCCGTGCTCCGGCCCGCTTCAGCTTTTTTTGATTCCTGGTTTCCGGGGG
GGCGGCGTGCCGCGTTGTCA

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: CBP; Protein fliY; Sulfate starvation-induced protein 7; SSI7 [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MKFVKALVLLLVTVMAVPAFAGDIDLAKKSVLNDILKRGELRVGFDAGYPPFEMTDKSGNFVGFDIDLGKELAKSMGVKF
VPVNTDFDGMIPSLLSGKFDVIISGMTLTQERNMKIGFSEPYLVMGQTVLVAAKHKGTVKSYKDLNDPKFIVVSRMGTTG
EEATKKYLPKATYKSFDKEVDCAMEVVNGRADAFVYDLPVNEVFQAQQGKDKTYLLNEPFTFEPMAVGYKQGDPDFANFI
NHFLRQLKNDGRYERMYNKWLRSDAWQSQLK

Sequences:

>Translated_271_residues
MKFVKALVLLLVTVMAVPAFAGDIDLAKKSVLNDILKRGELRVGFDAGYPPFEMTDKSGNFVGFDIDLGKELAKSMGVKF
VPVNTDFDGMIPSLLSGKFDVIISGMTLTQERNMKIGFSEPYLVMGQTVLVAAKHKGTVKSYKDLNDPKFIVVSRMGTTG
EEATKKYLPKATYKSFDKEVDCAMEVVNGRADAFVYDLPVNEVFQAQQGKDKTYLLNEPFTFEPMAVGYKQGDPDFANFI
NHFLRQLKNDGRYERMYNKWLRSDAWQSQLK
>Mature_271_residues
MKFVKALVLLLVTVMAVPAFAGDIDLAKKSVLNDILKRGELRVGFDAGYPPFEMTDKSGNFVGFDIDLGKELAKSMGVKF
VPVNTDFDGMIPSLLSGKFDVIISGMTLTQERNMKIGFSEPYLVMGQTVLVAAKHKGTVKSYKDLNDPKFIVVSRMGTTG
EEATKKYLPKATYKSFDKEVDCAMEVVNGRADAFVYDLPVNEVFQAQQGKDKTYLLNEPFTFEPMAVGYKQGDPDFANFI
NHFLRQLKNDGRYERMYNKWLRSDAWQSQLK

Specific function: Part of a binding-protein-dependent transport system for cystine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=256, Percent_Identity=30.078125, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1788649, Length=236, Percent_Identity=31.7796610169492, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1787085, Length=225, Percent_Identity=30.2222222222222, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787031, Length=226, Percent_Identity=29.2035398230088, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1787088, Length=232, Percent_Identity=28.8793103448276, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1788648, Length=234, Percent_Identity=29.0598290598291, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786876, Length=249, Percent_Identity=25.7028112449799, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 30470; Mature: 30470

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFVKALVLLLVTVMAVPAFAGDIDLAKKSVLNDILKRGELRVGFDAGYPPFEMTDKSGN
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEECCCCC
FVGFDIDLGKELAKSMGVKFVPVNTDFDGMIPSLLSGKFDVIISGMTLTQERNMKIGFSE
EEEEEHHHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCEEEEEECEEEECCCCCEECCCC
PYLVMGQTVLVAAKHKGTVKSYKDLNDPKFIVVSRMGTTGEEATKKYLPKATYKSFDKEV
CEEEECCEEEEEECCCCCHHHHHCCCCCCEEEEEECCCCCHHHHHHHCCCHHHHHHHHHH
DCAMEVVNGRADAFVYDLPVNEVFQAQQGKDKTYLLNEPFTFEPMAVGYKQGDPDFANFI
HHHHHHHCCCCCEEEEECCHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHH
NHFLRQLKNDGRYERMYNKWLRSDAWQSQLK
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKFVKALVLLLVTVMAVPAFAGDIDLAKKSVLNDILKRGELRVGFDAGYPPFEMTDKSGN
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEECCCCC
FVGFDIDLGKELAKSMGVKFVPVNTDFDGMIPSLLSGKFDVIISGMTLTQERNMKIGFSE
EEEEEHHHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCEEEEEECEEEECCCCCEECCCC
PYLVMGQTVLVAAKHKGTVKSYKDLNDPKFIVVSRMGTTGEEATKKYLPKATYKSFDKEV
CEEEECCEEEEEECCCCCHHHHHCCCCCCEEEEEECCCCCHHHHHHHCCCHHHHHHHHHH
DCAMEVVNGRADAFVYDLPVNEVFQAQQGKDKTYLLNEPFTFEPMAVGYKQGDPDFANFI
HHHHHHHCCCCCEEEEECCHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHH
NHFLRQLKNDGRYERMYNKWLRSDAWQSQLK
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]