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The map label for this gene is ygeV [C]

Identifier: 78356317

GI number: 78356317

Start: 1286947

End: 1288341

Strand: Reverse

Name: ygeV [C]

Synonym: Dde_1270

Alternate gene names: 78356317

Gene position: 1288341-1286947 (Counterclockwise)

Preceding gene: 78356319

Following gene: 78356311

Centisome position: 34.54

GC content: 55.7

Gene sequence:

>1395_bases
ATGCGTAAAGTAATACAACATATAGAAAGCATATTTGATGTATTAAGTGACGGGATATACATTACAGACAAATCCGGAAA
GACCCTGTTCGTCAACAAACGGTACGAACAGCTTACCGGCCTGCAGATGAAAGACATCAAGGGACTGAATGTCAGCACCC
TGCTGGAAGAAGGTGTCTTTGACATCATTCTGAACCCAGAAATAGTCCGCACACGCCGCCCGGCCACATCCGTGCAGAAT
GTCCGGCAGAGCAAAAAGGTCATCCTGCGCGGCTATCCTGTGTTTGACGATGAAGGTGAAGTCTGTCTTGTGGTCACATT
CGCACGCGACATCACCATGATAACCCAGTTCCGCGACCAGATAGCCCAGCAGCGCGAGCTGATAGACTCGTTCAACGAGC
GCATGGAACACATGGCGCAGGAGCAGACCCGCCACCCGCGCCCCGTATTTGAAAGTCATGCCATGAAACGGGTTCTGGAA
CTGCTGCAGCGCGTGGCTTCCACCGATGCCACCATGCTTATTCTGGGTGAAACAGGGGTGGGCAAAGATGTGATTGCCCG
TCTTGCCCATGAATACAGTCCCCGCGACGGCAAGCTGTTCATGAAGGTGGACTGCGGCAGCATAGCTGAAAATCTTATAG
AATCTGAATTGTTCGGCTATGTTCCGGGTGCGTTTTCCGGAGCAAGCAGCAAAGGGAAACCGGGTTACTTTGAAATAGCC
GACGGCGGTACAGTTTTTCTGGACGAAATAGGCGAGCTGCCGCTGCCCATGCAGGCAAAGCTGTTGCGCGTGCTGCAGGA
CAGAGAGGTCATGCGCGTGGGCGCCACGCAGCCCAAAGAGGTCAATGTGCGCATCATCGCCGCCACCAACCGCAATCTGG
CAAAAGAGGTGGAAGAAGGGCGGTTCCGCAGCGATCTTTTTTACCGCCTGAATGTGGCCGTGCTGCGGCTGCCGCCGCTG
CGCGAACGCAGCGACGACATTCTTCCGCTGGCAGACACGTTTCTTGACCGGTTCAATGCCAAATACCACCGCACCATGAC
CATGACCAGAGCCACTCGCCACGCTCTGGGCAACTACGGCTGGCCGGGCAATGTGCGTGAGATGCAGAATCTTATCCAGA
GCCTTGTGGTCACATGCGACGGGCGCTCTATCCGCCCCGAGCATCTGCCGCCGCATATCACCCGCGCAGGCAAAAGCACC
CCCTACACGCCCCCCGCCCCCGAAGACGAGCGCCCGCTGAAGGAAATCATGGCCGACATCGAACGGGAAATTCTGCAGGA
AGCCCTCAAACGTCACGGCTCGGTATGCAAGGTGGCATCCATGTACAAAATAAGCAGGACCACCCTGTTCAGAAAATTAC
GTGACGTACAGGACGGCACGCACGAAGACGCCTGA

Upstream 100 bases:

>100_bases
TGCACAAAATAATAAACACAGCATACAGTGCATCACATCAGCTTCATGCTTGGCATGGTAAAGCTGAAAGCGTGCTGCGC
TGCATTTTGCCGGCCATACA

Downstream 100 bases:

>100_bases
CGCCGCAAGGCCCGCACACGCCGGCGGTTTCCGCAGTGCCGCCTTTCTGCCCGGGGCCGGATTCCGCGGCCGCCATGAAC
ACATAAACCGCCCGCGGCAA

Product: Fis family transcriptional regulator

Products: NA

Alternate protein names: (S)-limonene 6-monooxygenase; (S)-limonene 7-monooxygenase; Carveol dehydrogenase; Perillyl-alcohol dehydrogenase [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MRKVIQHIESIFDVLSDGIYITDKSGKTLFVNKRYEQLTGLQMKDIKGLNVSTLLEEGVFDIILNPEIVRTRRPATSVQN
VRQSKKVILRGYPVFDDEGEVCLVVTFARDITMITQFRDQIAQQRELIDSFNERMEHMAQEQTRHPRPVFESHAMKRVLE
LLQRVASTDATMLILGETGVGKDVIARLAHEYSPRDGKLFMKVDCGSIAENLIESELFGYVPGAFSGASSKGKPGYFEIA
DGGTVFLDEIGELPLPMQAKLLRVLQDREVMRVGATQPKEVNVRIIAATNRNLAKEVEEGRFRSDLFYRLNVAVLRLPPL
RERSDDILPLADTFLDRFNAKYHRTMTMTRATRHALGNYGWPGNVREMQNLIQSLVVTCDGRSIRPEHLPPHITRAGKST
PYTPPAPEDERPLKEIMADIEREILQEALKRHGSVCKVASMYKISRTTLFRKLRDVQDGTHEDA

Sequences:

>Translated_464_residues
MRKVIQHIESIFDVLSDGIYITDKSGKTLFVNKRYEQLTGLQMKDIKGLNVSTLLEEGVFDIILNPEIVRTRRPATSVQN
VRQSKKVILRGYPVFDDEGEVCLVVTFARDITMITQFRDQIAQQRELIDSFNERMEHMAQEQTRHPRPVFESHAMKRVLE
LLQRVASTDATMLILGETGVGKDVIARLAHEYSPRDGKLFMKVDCGSIAENLIESELFGYVPGAFSGASSKGKPGYFEIA
DGGTVFLDEIGELPLPMQAKLLRVLQDREVMRVGATQPKEVNVRIIAATNRNLAKEVEEGRFRSDLFYRLNVAVLRLPPL
RERSDDILPLADTFLDRFNAKYHRTMTMTRATRHALGNYGWPGNVREMQNLIQSLVVTCDGRSIRPEHLPPHITRAGKST
PYTPPAPEDERPLKEIMADIEREILQEALKRHGSVCKVASMYKISRTTLFRKLRDVQDGTHEDA
>Mature_464_residues
MRKVIQHIESIFDVLSDGIYITDKSGKTLFVNKRYEQLTGLQMKDIKGLNVSTLLEEGVFDIILNPEIVRTRRPATSVQN
VRQSKKVILRGYPVFDDEGEVCLVVTFARDITMITQFRDQIAQQRELIDSFNERMEHMAQEQTRHPRPVFESHAMKRVLE
LLQRVASTDATMLILGETGVGKDVIARLAHEYSPRDGKLFMKVDCGSIAENLIESELFGYVPGAFSGASSKGKPGYFEIA
DGGTVFLDEIGELPLPMQAKLLRVLQDREVMRVGATQPKEVNVRIIAATNRNLAKEVEEGRFRSDLFYRLNVAVLRLPPL
RERSDDILPLADTFLDRFNAKYHRTMTMTRATRHALGNYGWPGNVREMQNLIQSLVVTCDGRSIRPEHLPPHITRAGKST
PYTPPAPEDERPLKEIMADIEREILQEALKRHGSVCKVASMYKISRTTLFRKLRDVQDGTHEDA

Specific function: Involved in limonene hydroxylation to a mixture of carveol and perillyl alcohol as well as in dehydrogenation of these products to carvone and perillyl aldehyde. Aromatic alcohols containing an isopropyl or isopropenyl group at ring position 4 also served

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789233, Length=317, Percent_Identity=41.9558359621451, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI1788550, Length=306, Percent_Identity=45.0980392156863, Blast_Score=245, Evalue=4e-66,
Organism=Escherichia coli, GI87082117, Length=319, Percent_Identity=42.9467084639498, Blast_Score=239, Evalue=4e-64,
Organism=Escherichia coli, GI1786524, Length=328, Percent_Identity=42.0731707317073, Blast_Score=231, Evalue=7e-62,
Organism=Escherichia coli, GI1790437, Length=307, Percent_Identity=43.6482084690554, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1787583, Length=321, Percent_Identity=41.1214953271028, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI1789087, Length=311, Percent_Identity=40.1929260450161, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI1790299, Length=327, Percent_Identity=39.4495412844037, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI87082152, Length=309, Percent_Identity=40.453074433657, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI1788905, Length=231, Percent_Identity=46.3203463203463, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI87081872, Length=291, Percent_Identity=40.893470790378, Blast_Score=198, Evalue=7e-52,
Organism=Escherichia coli, GI87081858, Length=461, Percent_Identity=23.644251626898, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1789828, Length=228, Percent_Identity=33.3333333333333, Blast_Score=121, Evalue=8e-29,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR002197
- InterPro:   IPR000014
- InterPro:   IPR002078 [H]

Pfam domain/function: PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: =1.14.13.48; =1.14.13.49; =1.1.1.243; =1.1.1.144 [H]

Molecular weight: Translated: 52703; Mature: 52703

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: PS50112 PAS ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKVIQHIESIFDVLSDGIYITDKSGKTLFVNKRYEQLTGLQMKDIKGLNVSTLLEEGVF
CHHHHHHHHHHHHHHHCCEEEECCCCCEEEEECHHHHHCCCCHHHCCCCCHHHHHHCCCE
DIILNPEIVRTRRPATSVQNVRQSKKVILRGYPVFDDEGEVCLVVTFARDITMITQFRDQ
EEEECCHHHHCCCCHHHHHHHHHHHHEEEECCCCCCCCCCEEEEEEEHHHHHHHHHHHHH
IAQQRELIDSFNERMEHMAQEQTRHPRPVFESHAMKRVLELLQRVASTDATMLILGETGV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
GKDVIARLAHEYSPRDGKLFMKVDCGSIAENLIESELFGYVPGAFSGASSKGKPGYFEIA
CHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEC
DGGTVFLDEIGELPLPMQAKLLRVLQDREVMRVGATQPKEVNVRIIAATNRNLAKEVEEG
CCCEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCHHHHHHHHC
RFRSDLFYRLNVAVLRLPPLRERSDDILPLADTFLDRFNAKYHRTMTMTRATRHALGNYG
CHHHHHHHEEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
WPGNVREMQNLIQSLVVTCDGRSIRPEHLPPHITRAGKSTPYTPPAPEDERPLKEIMADI
CCCCHHHHHHHHHHHHEECCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
EREILQEALKRHGSVCKVASMYKISRTTLFRKLRDVQDGTHEDA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRKVIQHIESIFDVLSDGIYITDKSGKTLFVNKRYEQLTGLQMKDIKGLNVSTLLEEGVF
CHHHHHHHHHHHHHHHCCEEEECCCCCEEEEECHHHHHCCCCHHHCCCCCHHHHHHCCCE
DIILNPEIVRTRRPATSVQNVRQSKKVILRGYPVFDDEGEVCLVVTFARDITMITQFRDQ
EEEECCHHHHCCCCHHHHHHHHHHHHEEEECCCCCCCCCCEEEEEEEHHHHHHHHHHHHH
IAQQRELIDSFNERMEHMAQEQTRHPRPVFESHAMKRVLELLQRVASTDATMLILGETGV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
GKDVIARLAHEYSPRDGKLFMKVDCGSIAENLIESELFGYVPGAFSGASSKGKPGYFEIA
CHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEC
DGGTVFLDEIGELPLPMQAKLLRVLQDREVMRVGATQPKEVNVRIIAATNRNLAKEVEEG
CCCEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCHHHHHHHHC
RFRSDLFYRLNVAVLRLPPLRERSDDILPLADTFLDRFNAKYHRTMTMTRATRHALGNYG
CHHHHHHHEEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
WPGNVREMQNLIQSLVVTCDGRSIRPEHLPPHITRAGKSTPYTPPAPEDERPLKEIMADI
CCCCHHHHHHHHHHHHEECCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
EREILQEALKRHGSVCKVASMYKISRTTLFRKLRDVQDGTHEDA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA