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The map label for this gene is maeB [H]

Identifier: 78356300

GI number: 78356300

Start: 1267517

End: 1268833

Strand: Reverse

Name: maeB [H]

Synonym: Dde_1253

Alternate gene names: 78356300

Gene position: 1268833-1267517 (Counterclockwise)

Preceding gene: 78356301

Following gene: 78356299

Centisome position: 34.01

GC content: 56.8

Gene sequence:

>1317_bases
ATGGCTCTGTTCACCAAGGAAGAAGCGCTGCACTATCACGAAGCCCCCCGCCACGGTAAGGTGGAGGTCATTCCCGTAAA
ACCGTGCCGCAACCAGAAAGACCTTTCCATGGCGTATTCGCCCGGCGTTGCCGAAGCATGCAAAGCCATCAAGGAAGACC
CTTCCAAGGCCGAGATATACACCGGCAGAAGCAATCTGGTGGCTGTGGTATCCGACGGCACCGCAGTGCTCGGGCTGGGC
AATATCGGCCCGCTGGCCGGAAAACCGGTCATGGAAGGCAAAGGCGTTCTTTTCAAAACATTTGCAGACATTGATGTTTT
TGACATCAACCTCGACGTTTCCAGTGCCGACCAGCTCATCGGCGTGGTCAAGGCGCTGGAACCCACCTTCGGCGGTATCA
ACCTTGAAGATATCAAGGCGCCGGAATGCTTCTACATTGAAGATACGCTGAAAAAAGAAATGAACATACCGGTGTTTCAC
GACGACCAGCACGGCACGGCTGTCATTTCCGGCGCCGGCCTGCTCAACGCGCTGGAAATAACCAACCGCAAAGCCGAAAA
CATGAAGGTCGTCGTGGTGGGAGCCGGGGCTGCAGGCATCGCATGCGCCAAGTTCTACGTTTCGCTGGGCGTGGATCCCG
CCAACATATACATGTTCGACTCACGCGGACTTATCCACAAGGGCCGTAAAGATCTGAACGAATACAAAGCTCTTTTCGCC
CAGCCGTCCGATGCGGGCACTCTGGCAGAGGTGATGAAGGGCGCAGACATGTTCCTGGGGCTTTCCACCAAAAACCTTGT
CTCGCAGGACATGGTGCGCTCCATGGCCAAAGACCCCGTTCTGTTTGCCATGGCCAACCCCGACCCCGAAATCACCTATC
CCGATGCCAAGCAGGCAAGCCCCAACTGCATCATGGGTACAGGGCGCTCCGACTTCCCCAATCAGGTCAACAACGTATCC
GGGTTCCCCTACATCTTCCGCGGGGCGCTGGATGTGGGTGCCACCGAAATCAACGAAGAAATGAAAGTGGCGGCAGCCCG
TTCGCTGGCAGCGCTGGCCAAAGAACCCGTACCTTCGGAGATATGCGACATGTACGGCGTGAAGGAACTGACCTTCGGCG
TCGACTATGTCATCCCCAAGCCTCTCGATCCCAGAGTTCTTGTCTGGGAAACCCCCGCTGTTGCCAAGGCAGCCATGGAC
TCCGGCGTGGCCAGAAAGCCCATCGCCGACATGGAAGCCTACAAAAAGCAGCTCACCACCCGCTTCGATGAGTCGCGCAA
GCGTATTGCGCGTGTTGTCGACAGCTACGGTCTTTAA

Upstream 100 bases:

>100_bases
CCGTCAAAGAGTTTCCGCTGCTGGTCATCAACGACTGTCACGGCGGCGAACTGTATGTAAAACCCGCTCTGGACCGCTAG
CAAGCAGGAGGAGTTCTCTC

Downstream 100 bases:

>100_bases
GCTCCCGGTTTAGGCTGTCCGCCATATCTGCCGACATTGCGGACACTTAAACGGCCATCCCGCGCCTGCCGCGGGATGGC
CCTCTCCCCTTGCGCGCTTT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NADP-ME [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MALFTKEEALHYHEAPRHGKVEVIPVKPCRNQKDLSMAYSPGVAEACKAIKEDPSKAEIYTGRSNLVAVVSDGTAVLGLG
NIGPLAGKPVMEGKGVLFKTFADIDVFDINLDVSSADQLIGVVKALEPTFGGINLEDIKAPECFYIEDTLKKEMNIPVFH
DDQHGTAVISGAGLLNALEITNRKAENMKVVVVGAGAAGIACAKFYVSLGVDPANIYMFDSRGLIHKGRKDLNEYKALFA
QPSDAGTLAEVMKGADMFLGLSTKNLVSQDMVRSMAKDPVLFAMANPDPEITYPDAKQASPNCIMGTGRSDFPNQVNNVS
GFPYIFRGALDVGATEINEEMKVAAARSLAALAKEPVPSEICDMYGVKELTFGVDYVIPKPLDPRVLVWETPAVAKAAMD
SGVARKPIADMEAYKKQLTTRFDESRKRIARVVDSYGL

Sequences:

>Translated_438_residues
MALFTKEEALHYHEAPRHGKVEVIPVKPCRNQKDLSMAYSPGVAEACKAIKEDPSKAEIYTGRSNLVAVVSDGTAVLGLG
NIGPLAGKPVMEGKGVLFKTFADIDVFDINLDVSSADQLIGVVKALEPTFGGINLEDIKAPECFYIEDTLKKEMNIPVFH
DDQHGTAVISGAGLLNALEITNRKAENMKVVVVGAGAAGIACAKFYVSLGVDPANIYMFDSRGLIHKGRKDLNEYKALFA
QPSDAGTLAEVMKGADMFLGLSTKNLVSQDMVRSMAKDPVLFAMANPDPEITYPDAKQASPNCIMGTGRSDFPNQVNNVS
GFPYIFRGALDVGATEINEEMKVAAARSLAALAKEPVPSEICDMYGVKELTFGVDYVIPKPLDPRVLVWETPAVAKAAMD
SGVARKPIADMEAYKKQLTTRFDESRKRIARVVDSYGL
>Mature_437_residues
ALFTKEEALHYHEAPRHGKVEVIPVKPCRNQKDLSMAYSPGVAEACKAIKEDPSKAEIYTGRSNLVAVVSDGTAVLGLGN
IGPLAGKPVMEGKGVLFKTFADIDVFDINLDVSSADQLIGVVKALEPTFGGINLEDIKAPECFYIEDTLKKEMNIPVFHD
DQHGTAVISGAGLLNALEITNRKAENMKVVVVGAGAAGIACAKFYVSLGVDPANIYMFDSRGLIHKGRKDLNEYKALFAQ
PSDAGTLAEVMKGADMFLGLSTKNLVSQDMVRSMAKDPVLFAMANPDPEITYPDAKQASPNCIMGTGRSDFPNQVNNVSG
FPYIFRGALDVGATEINEEMKVAAARSLAALAKEPVPSEICDMYGVKELTFGVDYVIPKPLDPRVLVWETPAVAKAAMDS
GVARKPIADMEAYKKQLTTRFDESRKRIARVVDSYGL

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI62420882, Length=349, Percent_Identity=28.3667621776504, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI62420880, Length=349, Percent_Identity=28.3667621776504, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI239049447, Length=349, Percent_Identity=28.3667621776504, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI4505143, Length=341, Percent_Identity=25.2199413489736, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI4505145, Length=345, Percent_Identity=25.5072463768116, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1788806, Length=441, Percent_Identity=56.6893424036281, Blast_Score=491, Evalue=1e-140,
Organism=Escherichia coli, GI87081919, Length=486, Percent_Identity=22.4279835390947, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17537199, Length=407, Percent_Identity=27.7641277641278, Blast_Score=81, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6322823, Length=353, Percent_Identity=28.0453257790368, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI78707242, Length=346, Percent_Identity=27.4566473988439, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI78707238, Length=346, Percent_Identity=27.4566473988439, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI78707240, Length=346, Percent_Identity=27.4566473988439, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24646388, Length=401, Percent_Identity=27.6807980049875, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI281362674, Length=410, Percent_Identity=27.0731707317073, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI281362672, Length=410, Percent_Identity=27.0731707317073, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI21356279, Length=410, Percent_Identity=27.0731707317073, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24646386, Length=401, Percent_Identity=27.6807980049875, Blast_Score=86, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.40 [H]

Molecular weight: Translated: 47255; Mature: 47124

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALFTKEEALHYHEAPRHGKVEVIPVKPCRNQKDLSMAYSPGVAEACKAIKEDPSKAEIY
CCCCCCHHHHHHHCCCCCCCEEEEECCCCCCCCCCCEEECCCHHHHHHHHHCCCCCCEEE
TGRSNLVAVVSDGTAVLGLGNIGPLAGKPVMEGKGVLFKTFADIDVFDINLDVSSADQLI
ECCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCHHHHH
GVVKALEPTFGGINLEDIKAPECFYIEDTLKKEMNIPVFHDDQHGTAVISGAGLLNALEI
HHHHHHCCCCCCCCHHHCCCCCEEEEHHHHHHHCCCCEEECCCCCEEEEECCCHHHHHHH
TNRKAENMKVVVVGAGAAGIACAKFYVSLGVDPANIYMFDSRGLIHKGRKDLNEYKALFA
HCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHC
QPSDAGTLAEVMKGADMFLGLSTKNLVSQDMVRSMAKDPVLFAMANPDPEITYPDAKQAS
CCCCCHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCC
PNCIMGTGRSDFPNQVNNVSGFPYIFRGALDVGATEINEEMKVAAARSLAALAKEPVPSE
CCEEEECCCCCCCCHHHCCCCCCCEEECHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
ICDMYGVKELTFGVDYVIPKPLDPRVLVWETPAVAKAAMDSGVARKPIADMEAYKKQLTT
HHHHCCCHHHHCCEEEECCCCCCCCEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHH
RFDESRKRIARVVDSYGL
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ALFTKEEALHYHEAPRHGKVEVIPVKPCRNQKDLSMAYSPGVAEACKAIKEDPSKAEIY
CCCCCHHHHHHHCCCCCCCEEEEECCCCCCCCCCCEEECCCHHHHHHHHHCCCCCCEEE
TGRSNLVAVVSDGTAVLGLGNIGPLAGKPVMEGKGVLFKTFADIDVFDINLDVSSADQLI
ECCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCHHHHH
GVVKALEPTFGGINLEDIKAPECFYIEDTLKKEMNIPVFHDDQHGTAVISGAGLLNALEI
HHHHHHCCCCCCCCHHHCCCCCEEEEHHHHHHHCCCCEEECCCCCEEEEECCCHHHHHHH
TNRKAENMKVVVVGAGAAGIACAKFYVSLGVDPANIYMFDSRGLIHKGRKDLNEYKALFA
HCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHC
QPSDAGTLAEVMKGADMFLGLSTKNLVSQDMVRSMAKDPVLFAMANPDPEITYPDAKQAS
CCCCCHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCC
PNCIMGTGRSDFPNQVNNVSGFPYIFRGALDVGATEINEEMKVAAARSLAALAKEPVPSE
CCEEEECCCCCCCCHHHCCCCCCCEEECHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
ICDMYGVKELTFGVDYVIPKPLDPRVLVWETPAVAKAAMDSGVARKPIADMEAYKKQLTT
HHHHCCCHHHHCCEEEECCCCCCCCEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHH
RFDESRKRIARVVDSYGL
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 36376 [H]