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The map label for this gene is fumA [H]

Identifier: 78356301

GI number: 78356301

Start: 1268854

End: 1269399

Strand: Reverse

Name: fumA [H]

Synonym: Dde_1254

Alternate gene names: 78356301

Gene position: 1269399-1268854 (Counterclockwise)

Preceding gene: 78356302

Following gene: 78356300

Centisome position: 34.03

GC content: 60.62

Gene sequence:

>546_bases
ATGGCCACCTATTATCTGCAGACCCCCCTGCGCGACCAGGATACCGAACAGCTTAAAGCGGGCGATGTCGTTTTTCTGAA
CGGCGAAATATTCACCGCCCGCGATGCCGCACACAAGCGCATGATGGAAACGCTGGAAAAGGGCGAGGCGCTGCCTTTTG
AAGCTCAGGGGGCCGTCATCTACTATGTGGGGCCCACACCTGCCCCCCCCGGCCGTCCCATCGGTTCGGCCGGTCCCACC
ACCAGCGGCCGCATGGACACATACACCCCCCGCATGCACAGTCTGGGCGTAAAGGCAAGCATAGGTAAGGGCAAGCGCAG
TCAGGACGTAATCGATGCGCTTAAACGGCACAAGGCTGTCTACTTCGGCGCCACCGGCGGTGCCGGAGCCCTGCTTTCGC
AATGCATCACCGCCGCAGAAGTGGTCGCATACGAAGAACTGGGGCCTGAAGCCATACGCCGGCTGACCGTCAAAGAGTTT
CCGCTGCTGGTCATCAACGACTGTCACGGCGGCGAACTGTATGTAAAACCCGCTCTGGACCGCTAG

Upstream 100 bases:

>100_bases
TTGCCGTTAAAATAGCCATGCATCCCTGCCACATCGCCAGCCTGCCGCTGGCCGTCAACGTGCAGTGCCATTCTTCGCGG
CACAAGGAGGTTGTATTCTA

Downstream 100 bases:

>100_bases
CAAGCAGGAGGAGTTCTCTCATGGCTCTGTTCACCAAGGAAGAAGCGCTGCACTATCACGAAGCCCCCCGCCACGGTAAG
GTGGAGGTCATTCCCGTAAA

Product: tartrate/fumarate subfamily Fe-S type hydro-lyase subunit beta

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 181; Mature: 180

Protein sequence:

>181_residues
MATYYLQTPLRDQDTEQLKAGDVVFLNGEIFTARDAAHKRMMETLEKGEALPFEAQGAVIYYVGPTPAPPGRPIGSAGPT
TSGRMDTYTPRMHSLGVKASIGKGKRSQDVIDALKRHKAVYFGATGGAGALLSQCITAAEVVAYEELGPEAIRRLTVKEF
PLLVINDCHGGELYVKPALDR

Sequences:

>Translated_181_residues
MATYYLQTPLRDQDTEQLKAGDVVFLNGEIFTARDAAHKRMMETLEKGEALPFEAQGAVIYYVGPTPAPPGRPIGSAGPT
TSGRMDTYTPRMHSLGVKASIGKGKRSQDVIDALKRHKAVYFGATGGAGALLSQCITAAEVVAYEELGPEAIRRLTVKEF
PLLVINDCHGGELYVKPALDR
>Mature_180_residues
ATYYLQTPLRDQDTEQLKAGDVVFLNGEIFTARDAAHKRMMETLEKGEALPFEAQGAVIYYVGPTPAPPGRPIGSAGPTT
SGRMDTYTPRMHSLGVKASIGKGKRSQDVIDALKRHKAVYFGATGGAGALLSQCITAAEVVAYEELGPEAIRRLTVKEFP
LLVINDCHGGELYVKPALDR

Specific function: It functions as an aerobic enzyme in the citric acid cycle. It accounts for about 80% of the fumarase activity when the bacteria grows aerobically [H]

COG id: COG1838

COG function: function code C; Tartrate dehydratase beta subunit/Fumarate hydratase class I, C-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=156, Percent_Identity=41.6666666666667, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI1790564, Length=151, Percent_Identity=42.3841059602649, Blast_Score=120, Evalue=5e-29,
Organism=Escherichia coli, GI1789443, Length=173, Percent_Identity=32.9479768786127, Blast_Score=94, Evalue=5e-21,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 19537; Mature: 19405

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATYYLQTPLRDQDTEQLKAGDVVFLNGEIFTARDAAHKRMMETLEKGEALPFEAQGAVI
CCEEEECCCCCCCCHHHHCCCCEEEECCEEEECHHHHHHHHHHHHHHCCCCCEECCCEEE
YYVGPTPAPPGRPIGSAGPTTSGRMDTYTPRMHSLGVKASIGKGKRSQDVIDALKRHKAV
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCEEECCCCCCHHHHHHHHHHCCEE
YFGATGGAGALLSQCITAAEVVAYEELGPEAIRRLTVKEFPLLVINDCHGGELYVKPALD
EEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEECCCCCEEEEECCCC
R
C
>Mature Secondary Structure 
ATYYLQTPLRDQDTEQLKAGDVVFLNGEIFTARDAAHKRMMETLEKGEALPFEAQGAVI
CEEEECCCCCCCCHHHHCCCCEEEECCEEEECHHHHHHHHHHHHHHCCCCCEECCCEEE
YYVGPTPAPPGRPIGSAGPTTSGRMDTYTPRMHSLGVKASIGKGKRSQDVIDALKRHKAV
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCEEECCCCCCHHHHHHHHHHCCEE
YFGATGGAGALLSQCITAAEVVAYEELGPEAIRRLTVKEFPLLVINDCHGGELYVKPALD
EEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEECCCCCEEEEECCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 1879695 [H]