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The map label for this gene is ptsI [H]

Identifier: 78356227

GI number: 78356227

Start: 1207335

End: 1209854

Strand: Direct

Name: ptsI [H]

Synonym: Dde_1180

Alternate gene names: 78356227

Gene position: 1207335-1209854 (Clockwise)

Preceding gene: 78356226

Following gene: 78356235

Centisome position: 32.37

GC content: 64.44

Gene sequence:

>2520_bases
ATGGTCGGCCTTGTAATTGTTTCTCACAGTGCCGTTCTGGCAGAAGGAGTGCGCGAGCTTGCCCTGCAGATGACGCAGGG
CAGGGTGCGTATTGCTCTGGCAGGCGGTGTGGATGATGCAGAACACCCCATAGGCACCGATCCCACACGTGTGATGGCCG
CACTGGAGGAAGTGCAGGACGGTGACGGCGTGCTGGTGCTGATGGATCTGGGCAGTGCGCTGATGAGTGCGGAAACGGCG
CTTGACCTGCTGCCGCCTGAAGTGACGGCCAATGTGCGTCTTTCGGCGGCTCCGCTGATCGAAGGTTGTATTGCTGCCGC
GGTGCAGGCCGCGGCGGGTGCCTCGCTGGACGATGTTCAGGCCGAAGCGGAGTCGGCCCTTGCGGCTAAATCGGCTCAGC
TCGGTTCGGCGGTGCCTGACATGGCTGCGTCTTCGGCCGGAGCTGCCGGTGCAGTGCCCGATGCCGCTGCGGAGCATGTC
CGGACGCTTGCTGTGACGGTGCCCAACAGACTGGGGCTGCATGCCCGTCCGGCATCCCGCATTGTGGGGGCGCTGGCAGG
GTTGGACGCCGATGTGCGTCTGGCGCATGGAGGGCATGTGGTCAACGCCCGTTCACTCAACCAGATAGCCACGCTGGCGG
TGCGCCTCGGCGATTCCATCGAGTTCCGCGCAGCCGGTGCCGACGCACAGCAGGCGCTTGATGCCCTGCAGGCACTGCAT
GCGGCAAACTTTGGCGATCCGCGTGAACCGGAAGAAGGGCAGGCGGACAGCCATGGTGCGGCACCGGTCCGGCCTGCAGG
TGCCGTAGCCGCCTCGGCCGGGTATGCAGCGGGACCGGCTGTGCGCTTCAGAGCGGCATTGCCGGAAGTGGCGGAAATTC
AGGTTGACGATCAGGCGGCCGAAAAGCGTGTTCTGCAGGATGCTCTGAAGCAGGCCGCGGACGAGATTGCGCTTGTGGAA
AAGCAGACGGCCGGAAACGTGGGCGGCGCGGAAAGCGAAATTTTCGGCATGCACCGGATGATGCTGCAGGATCCTGAATT
GAGGGATGCCGCGCTGCATGAACTGGAAGAGCGCCGGTGCAACGCGGCGGCGGCGTGGCGGCGTGTCATCGTTTCCACCG
CGGAACGCTACCGCGGTCTGGATGACGATTTTATGCGCGCCCGTGCGGCTGACGTGCTGGATGTGGGAGCACGCGTGCTG
CGTATCATCACCGGAGAGGCTCCGCAGGGGCCGGATATCGCCGAACCCAGTGTGCTGCTGGCAGCCGATCTGGGGCCTTC
GGACATGGCGCGGCTTGATGTGACAAAAGTTCTGGGTATTGTTACCGAGCAGGGCGGTGCCACATCGCATGCCGCCATTC
TGGCGCGTTCCATGGGCATACCCGCCGTGGCGGGGGCCGCAGCGCAGATAGCGGGCGTGACCGACGGCACTCCTGTGGGG
CTGGACGGCAATACCGGTGAGGTATGGGCTCCGGCCGACGAACACACTGTGCAAAGACTGCGCGGTCTGCGCCGTCAATG
GGAAGAATCGCTGCGCAGTGAGCGTGAAAAGGCCGCCGCACCGGCTGTCACCCGTGACGGCAGGGCTGTGCGGGTTCATG
CGAATATCGGTTCACCGGCGGACGCGCATGCCGCTGTGCGCAACGGGGCCGAAGGTGTGGGGCTGTTCCGCACGGAGTTC
CTCTTTCTCGACAGGCCTTCCGCTCCTACAGAAGACGAACAGACGGCCGCGTATACTGAAGCTGCTCAGGCTCTTGACGG
CCGGCCGGTTATTATCCGCACGCTGGATATCGGGGGCGACAAGCCTGTACCGTATCTGCAGCAGGGGCAGGCGGCCGAAG
CCAATCCTTTTCTGGGAGTGCGCGGCATACGGTTCTGCCTTGTGCATGAAGACCTGTTCGTGACGCAGCTCAGGGCGCTT
CTGCGCGCTGCGGCGGATCACCCCGTGCAGATCATGTTTCCCATGGTGGCTCATCCGGGCGAGCTGCGGGCGGCCAGAAC
GTTGCTGGAAAAAGCCCGCGCACAGCTGCTGGCCGAGGGGCGTCCGTGCGGACATGATGTGCCCGTGGGGATTATGATAG
AAGTACCCGCCGCGGTGGCTCTGGCAGATCAGCTGGCTGCTGAAGCGGACTTTTTCAGCATCGGCACCAATGATCTGGCC
CAGTATGTCATGGCGGCAGACAGAGGCAACAGCGCTGTGGCGGCTTTGTCCGATGCCCTGCATCCCGCTGTGTTGCGCCA
GATTCAGCAGACAGTGGAGGCCGGTCACGCGGCCGGAATTCATGTGGGCATGTGCGGAGAGCTGGCAGGCGCGCCGCTGG
CAGTGCCCCTGCTGGCCGCCATGAATCTTGATTCGCTGAGCATGAGCGGTCCGGCCGTGCCCCGTGCCAAGGAAATCATC
CGGAGCATTGATGCCGCTGCGTTGCAGCCGCTGGTGGAACAGGTGCTGGAACTGCCCGATGCTGCGGCGGTGCGTAATCT
GCTGCAGCAGGCCGCGGGGGAATCTGCGGACCGTGCGTGA

Upstream 100 bases:

>100_bases
AGCTATCTGGGCGAACGCAGCATCGGTCACAAAGATCCCGGCGGGGCTTCCTCGTGCATGATGCTCGGCGCGTTGCGTCA
GAGTATCGGGGGATAGCCGC

Downstream 100 bases:

>100_bases
GAGTAAGGCCCGCGGTGCTGACGGCGCAAGGCGTGCCGCCGGCTGGCCGCAGGATAACGGCAGGCGCGGTGCGCGTGGCC
CTTTCCGGTTGCGGTACATG

Product: phosphoenolpyruvate--protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 839; Mature: 839

Protein sequence:

>839_residues
MVGLVIVSHSAVLAEGVRELALQMTQGRVRIALAGGVDDAEHPIGTDPTRVMAALEEVQDGDGVLVLMDLGSALMSAETA
LDLLPPEVTANVRLSAAPLIEGCIAAAVQAAAGASLDDVQAEAESALAAKSAQLGSAVPDMAASSAGAAGAVPDAAAEHV
RTLAVTVPNRLGLHARPASRIVGALAGLDADVRLAHGGHVVNARSLNQIATLAVRLGDSIEFRAAGADAQQALDALQALH
AANFGDPREPEEGQADSHGAAPVRPAGAVAASAGYAAGPAVRFRAALPEVAEIQVDDQAAEKRVLQDALKQAADEIALVE
KQTAGNVGGAESEIFGMHRMMLQDPELRDAALHELEERRCNAAAAWRRVIVSTAERYRGLDDDFMRARAADVLDVGARVL
RIITGEAPQGPDIAEPSVLLAADLGPSDMARLDVTKVLGIVTEQGGATSHAAILARSMGIPAVAGAAAQIAGVTDGTPVG
LDGNTGEVWAPADEHTVQRLRGLRRQWEESLRSEREKAAAPAVTRDGRAVRVHANIGSPADAHAAVRNGAEGVGLFRTEF
LFLDRPSAPTEDEQTAAYTEAAQALDGRPVIIRTLDIGGDKPVPYLQQGQAAEANPFLGVRGIRFCLVHEDLFVTQLRAL
LRAAADHPVQIMFPMVAHPGELRAARTLLEKARAQLLAEGRPCGHDVPVGIMIEVPAAVALADQLAAEADFFSIGTNDLA
QYVMAADRGNSAVAALSDALHPAVLRQIQQTVEAGHAAGIHVGMCGELAGAPLAVPLLAAMNLDSLSMSGPAVPRAKEII
RSIDAAALQPLVEQVLELPDAAAVRNLLQQAAGESADRA

Sequences:

>Translated_839_residues
MVGLVIVSHSAVLAEGVRELALQMTQGRVRIALAGGVDDAEHPIGTDPTRVMAALEEVQDGDGVLVLMDLGSALMSAETA
LDLLPPEVTANVRLSAAPLIEGCIAAAVQAAAGASLDDVQAEAESALAAKSAQLGSAVPDMAASSAGAAGAVPDAAAEHV
RTLAVTVPNRLGLHARPASRIVGALAGLDADVRLAHGGHVVNARSLNQIATLAVRLGDSIEFRAAGADAQQALDALQALH
AANFGDPREPEEGQADSHGAAPVRPAGAVAASAGYAAGPAVRFRAALPEVAEIQVDDQAAEKRVLQDALKQAADEIALVE
KQTAGNVGGAESEIFGMHRMMLQDPELRDAALHELEERRCNAAAAWRRVIVSTAERYRGLDDDFMRARAADVLDVGARVL
RIITGEAPQGPDIAEPSVLLAADLGPSDMARLDVTKVLGIVTEQGGATSHAAILARSMGIPAVAGAAAQIAGVTDGTPVG
LDGNTGEVWAPADEHTVQRLRGLRRQWEESLRSEREKAAAPAVTRDGRAVRVHANIGSPADAHAAVRNGAEGVGLFRTEF
LFLDRPSAPTEDEQTAAYTEAAQALDGRPVIIRTLDIGGDKPVPYLQQGQAAEANPFLGVRGIRFCLVHEDLFVTQLRAL
LRAAADHPVQIMFPMVAHPGELRAARTLLEKARAQLLAEGRPCGHDVPVGIMIEVPAAVALADQLAAEADFFSIGTNDLA
QYVMAADRGNSAVAALSDALHPAVLRQIQQTVEAGHAAGIHVGMCGELAGAPLAVPLLAAMNLDSLSMSGPAVPRAKEII
RSIDAAALQPLVEQVLELPDAAAVRNLLQQAAGESADRA
>Mature_839_residues
MVGLVIVSHSAVLAEGVRELALQMTQGRVRIALAGGVDDAEHPIGTDPTRVMAALEEVQDGDGVLVLMDLGSALMSAETA
LDLLPPEVTANVRLSAAPLIEGCIAAAVQAAAGASLDDVQAEAESALAAKSAQLGSAVPDMAASSAGAAGAVPDAAAEHV
RTLAVTVPNRLGLHARPASRIVGALAGLDADVRLAHGGHVVNARSLNQIATLAVRLGDSIEFRAAGADAQQALDALQALH
AANFGDPREPEEGQADSHGAAPVRPAGAVAASAGYAAGPAVRFRAALPEVAEIQVDDQAAEKRVLQDALKQAADEIALVE
KQTAGNVGGAESEIFGMHRMMLQDPELRDAALHELEERRCNAAAAWRRVIVSTAERYRGLDDDFMRARAADVLDVGARVL
RIITGEAPQGPDIAEPSVLLAADLGPSDMARLDVTKVLGIVTEQGGATSHAAILARSMGIPAVAGAAAQIAGVTDGTPVG
LDGNTGEVWAPADEHTVQRLRGLRRQWEESLRSEREKAAAPAVTRDGRAVRVHANIGSPADAHAAVRNGAEGVGLFRTEF
LFLDRPSAPTEDEQTAAYTEAAQALDGRPVIIRTLDIGGDKPVPYLQQGQAAEANPFLGVRGIRFCLVHEDLFVTQLRAL
LRAAADHPVQIMFPMVAHPGELRAARTLLEKARAQLLAEGRPCGHDVPVGIMIEVPAAVALADQLAAEADFFSIGTNDLA
QYVMAADRGNSAVAALSDALHPAVLRQIQQTVEAGHAAGIHVGMCGELAGAPLAVPLLAAMNLDSLSMSGPAVPRAKEII
RSIDAAALQPLVEQVLELPDAAAVRNLLQQAAGESADRA

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=581, Percent_Identity=40.2753872633391, Blast_Score=388, Evalue=1e-109,
Organism=Escherichia coli, GI48994992, Length=691, Percent_Identity=34.1534008683068, Blast_Score=318, Evalue=1e-87,
Organism=Escherichia coli, GI1788726, Length=688, Percent_Identity=32.9941860465116, Blast_Score=308, Evalue=7e-85,
Organism=Escherichia coli, GI226510935, Length=493, Percent_Identity=39.7565922920893, Blast_Score=306, Evalue=4e-84,
Organism=Escherichia coli, GI1789193, Length=552, Percent_Identity=35.1449275362319, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI1787994, Length=402, Percent_Identity=27.6119402985075, Blast_Score=110, Evalue=3e-25,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 86850; Mature: 86850

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00369 PTS_HPR_HIS ; PS51096 PTS_EIIA_TYPE_4 ; PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGLVIVSHSAVLAEGVRELALQMTQGRVRIALAGGVDDAEHPIGTDPTRVMAALEEVQD
CEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCC
GDGVLVLMDLGSALMSAETALDLLPPEVTANVRLSAAPLIEGCIAAAVQAAAGASLDDVQ
CCCEEEEEECCHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHHHHCCCCHHHHH
AEAESALAAKSAQLGSAVPDMAASSAGAAGAVPDAAAEHVRTLAVTVPNRLGLHARPASR
HHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHEEEEECCCCCCCCCCHHHH
IVGALAGLDADVRLAHGGHVVNARSLNQIATLAVRLGDSIEFRAAGADAQQALDALQALH
HHHHHHCCCCCEEEECCCEEECHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHH
AANFGDPREPEEGQADSHGAAPVRPAGAVAASAGYAAGPAVRFRAALPEVAEIQVDDQAA
HCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHCCCHHEEEECHHHH
EKRVLQDALKQAADEIALVEKQTAGNVGGAESEIFGMHRMMLQDPELRDAALHELEERRC
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
NAAAAWRRVIVSTAERYRGLDDDFMRARAADVLDVGARVLRIITGEAPQGPDIAEPSVLL
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEE
AADLGPSDMARLDVTKVLGIVTEQGGATSHAAILARSMGIPAVAGAAAQIAGVTDGTPVG
EECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHCCHHHCCCCCCCCCCC
LDGNTGEVWAPADEHTVQRLRGLRRQWEESLRSEREKAAAPAVTRDGRAVRVHANIGSPA
CCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCH
DAHAAVRNGAEGVGLFRTEFLFLDRPSAPTEDEQTAAYTEAAQALDGRPVIIRTLDIGGD
HHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCC
KPVPYLQQGQAAEANPFLGVRGIRFCLVHEDLFVTQLRALLRAAADHPVQIMFPMVAHPG
CCCCHHHCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCC
ELRAARTLLEKARAQLLAEGRPCGHDVPVGIMIEVPAAVALADQLAAEADFFSIGTNDLA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCEECCHHHHH
QYVMAADRGNSAVAALSDALHPAVLRQIQQTVEAGHAAGIHVGMCGELAGAPLAVPLLAA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHCCCCHHHHHHHH
MNLDSLSMSGPAVPRAKEIIRSIDAAALQPLVEQVLELPDAAAVRNLLQQAAGESADRA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MVGLVIVSHSAVLAEGVRELALQMTQGRVRIALAGGVDDAEHPIGTDPTRVMAALEEVQD
CEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCC
GDGVLVLMDLGSALMSAETALDLLPPEVTANVRLSAAPLIEGCIAAAVQAAAGASLDDVQ
CCCEEEEEECCHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHHHHCCCCHHHHH
AEAESALAAKSAQLGSAVPDMAASSAGAAGAVPDAAAEHVRTLAVTVPNRLGLHARPASR
HHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHEEEEECCCCCCCCCCHHHH
IVGALAGLDADVRLAHGGHVVNARSLNQIATLAVRLGDSIEFRAAGADAQQALDALQALH
HHHHHHCCCCCEEEECCCEEECHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHH
AANFGDPREPEEGQADSHGAAPVRPAGAVAASAGYAAGPAVRFRAALPEVAEIQVDDQAA
HCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHCCCHHEEEECHHHH
EKRVLQDALKQAADEIALVEKQTAGNVGGAESEIFGMHRMMLQDPELRDAALHELEERRC
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
NAAAAWRRVIVSTAERYRGLDDDFMRARAADVLDVGARVLRIITGEAPQGPDIAEPSVLL
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEE
AADLGPSDMARLDVTKVLGIVTEQGGATSHAAILARSMGIPAVAGAAAQIAGVTDGTPVG
EECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHCCHHHCCCCCCCCCCC
LDGNTGEVWAPADEHTVQRLRGLRRQWEESLRSEREKAAAPAVTRDGRAVRVHANIGSPA
CCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCH
DAHAAVRNGAEGVGLFRTEFLFLDRPSAPTEDEQTAAYTEAAQALDGRPVIIRTLDIGGD
HHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCC
KPVPYLQQGQAAEANPFLGVRGIRFCLVHEDLFVTQLRALLRAAADHPVQIMFPMVAHPG
CCCCHHHCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCC
ELRAARTLLEKARAQLLAEGRPCGHDVPVGIMIEVPAAVALADQLAAEADFFSIGTNDLA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCEECCHHHHH
QYVMAADRGNSAVAALSDALHPAVLRQIQQTVEAGHAAGIHVGMCGELAGAPLAVPLLAA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHCCCCHHHHHHHH
MNLDSLSMSGPAVPRAKEIIRSIDAAALQPLVEQVLELPDAAAVRNLLQQAAGESADRA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]