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The map label for this gene is dhaK [H]

Identifier: 78356225

GI number: 78356225

Start: 1205541

End: 1206605

Strand: Direct

Name: dhaK [H]

Synonym: Dde_1178

Alternate gene names: 78356225

Gene position: 1205541-1206605 (Clockwise)

Preceding gene: 78356224

Following gene: 78356226

Centisome position: 32.32

GC content: 57.75

Gene sequence:

>1065_bases
ATGAAAAAGCTGATTAATGCCGTTGATGATGTCGTCCGGGAGCAGCTGCAGGGCATGGCCGCCGCTCATCCCGAACTGCT
GGTCCATCTGGACCCGCATTATGTGTGCCGCAGAGATATTCCCGAAGGCAAGGTGGCTGTTGTTTCCGGCGGGGGCAGCG
GGCACGAACCCATGCACGGCGGTTTTGTGGGGCATGGCATGCTGGACGGGGCCTGCCCCGGAGAGGTGTTTACATCGCCC
ACGCCCGACCAGATGTATGAATGCGCCAAAGCTGTGGACCGCGGTGCCGGCGTGCTGTTCATTGTCAAAAACTACACCGG
CGACGTGATGAATTTTGAAACCGCCGCCGAACTGTGCCATGCCGATGGCATCAAGGTGCAGAACATACTTATCGACGACG
ACGTTGCCGTGAAGGACAGCCTGTATACTGCGGGACGCCGCGGGGTGGGCACCACCGTGCTGGCGGAAAAAATTGTAGGC
GCCGCAGCCGAAGCCGGTTATTCGCTGGAACAATGCGCCGACCTGTGCCGCAGGGTGAACAGTTGCGGGCGCTCCATGGG
CGTTGCACTGACATCCTGCACGGTGCCTGCGGCGGGCAAGCCTACTTTTGAGCTGGCCGACGGCGAGATTGAAATCGGCA
TCGGTATTCATGGTGAACCCGGAACGCACCGCAGCTCCATGCAGACGGCGGATGAGCTGACAACCATTGTGGCTCAGGGC
ATTCTGGACGACCCTGCCTATACCCGCACCGTGCGTGAACTCAATCATGATACCGGCCAGTGGGAAGACAAAAAACTTAC
CGATGAGCCTTTTGCCAAAGGCGACCGGGTTATTGCTTTTGTAAACAGCATGGGCGGCACCCCCGTAAGCGAACTGTACG
CCGTTTACCGCAAGCTGGATGCCATCTGCCGCGACAGGGGCATCGTCATCGAACGCAATCTTATCGGCCCCTACATCACT
TCACTGGAAATGCAGGGATTTTCCATCACGCTGCTTAAAGTGGATGATGAAATGCTGAAGTTCTGGGACGCCGGAGCAAA
CACCCCCGGTTTTGTAAAAATCTGA

Upstream 100 bases:

>100_bases
TGCGAGCCGCTGGATTCGCATGAACGACCTGCCGGATGCAGGTCTGGTCAGACTCTCTGAGGCGAGACCGGATGGCTCAT
AACCAAACGGAGAAAAATCG

Downstream 100 bases:

>100_bases
TAACAACATATCCGCCTGCGGGGTCTGCACGGCCCCGCGGCGCAAAGCATATACCGGCCGGCATCGCTGCCGGACCGCCG
GAGATTTTCCGCATGCAGAT

Product: dihydroxyacetone kinase subunit DhaK

Products: ADP; glycerone phosphate

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MKKLINAVDDVVREQLQGMAAAHPELLVHLDPHYVCRRDIPEGKVAVVSGGGSGHEPMHGGFVGHGMLDGACPGEVFTSP
TPDQMYECAKAVDRGAGVLFIVKNYTGDVMNFETAAELCHADGIKVQNILIDDDVAVKDSLYTAGRRGVGTTVLAEKIVG
AAAEAGYSLEQCADLCRRVNSCGRSMGVALTSCTVPAAGKPTFELADGEIEIGIGIHGEPGTHRSSMQTADELTTIVAQG
ILDDPAYTRTVRELNHDTGQWEDKKLTDEPFAKGDRVIAFVNSMGGTPVSELYAVYRKLDAICRDRGIVIERNLIGPYIT
SLEMQGFSITLLKVDDEMLKFWDAGANTPGFVKI

Sequences:

>Translated_354_residues
MKKLINAVDDVVREQLQGMAAAHPELLVHLDPHYVCRRDIPEGKVAVVSGGGSGHEPMHGGFVGHGMLDGACPGEVFTSP
TPDQMYECAKAVDRGAGVLFIVKNYTGDVMNFETAAELCHADGIKVQNILIDDDVAVKDSLYTAGRRGVGTTVLAEKIVG
AAAEAGYSLEQCADLCRRVNSCGRSMGVALTSCTVPAAGKPTFELADGEIEIGIGIHGEPGTHRSSMQTADELTTIVAQG
ILDDPAYTRTVRELNHDTGQWEDKKLTDEPFAKGDRVIAFVNSMGGTPVSELYAVYRKLDAICRDRGIVIERNLIGPYIT
SLEMQGFSITLLKVDDEMLKFWDAGANTPGFVKI
>Mature_354_residues
MKKLINAVDDVVREQLQGMAAAHPELLVHLDPHYVCRRDIPEGKVAVVSGGGSGHEPMHGGFVGHGMLDGACPGEVFTSP
TPDQMYECAKAVDRGAGVLFIVKNYTGDVMNFETAAELCHADGIKVQNILIDDDVAVKDSLYTAGRRGVGTTVLAEKIVG
AAAEAGYSLEQCADLCRRVNSCGRSMGVALTSCTVPAAGKPTFELADGEIEIGIGIHGEPGTHRSSMQTADELTTIVAQG
ILDDPAYTRTVRELNHDTGQWEDKKLTDEPFAKGDRVIAFVNSMGGTPVSELYAVYRKLDAICRDRGIVIERNLIGPYIT
SLEMQGFSITLLKVDDEMLKFWDAGANTPGFVKI

Specific function: Dihydroxyacetone binding subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone. Binds covalently dihydroxyacetone in hemiaminal linkage. Acts also as a corepressor of dhaR by binding to its sensor domain, in the

COG id: COG2376

COG function: function code G; Dihydroxyacetone kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DhaK domain [H]

Homologues:

Organism=Homo sapiens, GI20149621, Length=351, Percent_Identity=42.1652421652422, Blast_Score=236, Evalue=2e-62,
Organism=Escherichia coli, GI87081857, Length=352, Percent_Identity=60.5113636363636, Blast_Score=446, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17565018, Length=358, Percent_Identity=34.3575418994413, Blast_Score=194, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6321055, Length=353, Percent_Identity=37.9603399433428, Blast_Score=198, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6323570, Length=364, Percent_Identity=34.3406593406593, Blast_Score=175, Evalue=8e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004006
- InterPro:   IPR012736 [H]

Pfam domain/function: PF02733 Dak1 [H]

EC number: 2.7.1.29

Molecular weight: Translated: 37999; Mature: 37999

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLINAVDDVVREQLQGMAAAHPELLVHLDPHYVCRRDIPEGKVAVVSGGGSGHEPMHG
CHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHCCCCCCCEEEEECCCCCCCCCCC
GFVGHGMLDGACPGEVFTSPTPDQMYECAKAVDRGAGVLFIVKNYTGDVMNFETAAELCH
CEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHH
ADGIKVQNILIDDDVAVKDSLYTAGRRGVGTTVLAEKIVGAAAEAGYSLEQCADLCRRVN
CCCCEEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
SCGRSMGVALTSCTVPAAGKPTFELADGEIEIGIGIHGEPGTHRSSMQTADELTTIVAQG
HHHHHHCEEEEEECCCCCCCCCEEECCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
ILDDPAYTRTVRELNHDTGQWEDKKLTDEPFAKGDRVIAFVNSMGGTPVSELYAVYRKLD
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHH
AICRDRGIVIERNLIGPYITSLEMQGFSITLLKVDDEMLKFWDAGANTPGFVKI
HHHHCCCEEEECCCCCHHHHHEEECCEEEEEEECCHHHHHHHHCCCCCCCEEEC
>Mature Secondary Structure
MKKLINAVDDVVREQLQGMAAAHPELLVHLDPHYVCRRDIPEGKVAVVSGGGSGHEPMHG
CHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHCCCCCCCEEEEECCCCCCCCCCC
GFVGHGMLDGACPGEVFTSPTPDQMYECAKAVDRGAGVLFIVKNYTGDVMNFETAAELCH
CEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHH
ADGIKVQNILIDDDVAVKDSLYTAGRRGVGTTVLAEKIVGAAAEAGYSLEQCADLCRRVN
CCCCEEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
SCGRSMGVALTSCTVPAAGKPTFELADGEIEIGIGIHGEPGTHRSSMQTADELTTIVAQG
HHHHHHCEEEEEECCCCCCCCCEEECCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
ILDDPAYTRTVRELNHDTGQWEDKKLTDEPFAKGDRVIAFVNSMGGTPVSELYAVYRKLD
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHH
AICRDRGIVIERNLIGPYITSLEMQGFSITLLKVDDEMLKFWDAGANTPGFVKI
HHHHCCCEEEECCCCCHHHHHEEECCEEEEEEECCHHHHHHHHCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; glycerone

Specific reaction: ATP + glycerone = ADP + glycerone phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]