Click here to switch to the map view.

The map label for this gene is ycjG [H]

Identifier: 78356224

GI number: 78356224

Start: 1203937

End: 1205289

Strand: Direct

Name: ycjG [H]

Synonym: Dde_1177

Alternate gene names: 78356224

Gene position: 1203937-1205289 (Clockwise)

Preceding gene: 78356221

Following gene: 78356225

Centisome position: 32.28

GC content: 62.01

Gene sequence:

>1353_bases
TTGGGCAATGGAAAAAAGAAGCTCCGGGCGTCGGTGCCGAAGGTGGGGGGGGGATACCTTGGGCGCCGCGTTTCCCGGAG
CTGGTCGGAAGGCAGTGTTACAGGCGGTACCGCGCAAAGACGACCCGCCGGAAGAATCAAGCAAGTTCTGTGCAGACAGG
GCAATGGCCGGTGTATTCAGGGTGATAAGTTGTTGCTGCGGCATCTTGCCCCTGCAAAAAATGTAAATATGTCCGACATG
ATGACTGTCTATCCGACACCGGCTGCCGTGGTGTGGCATTGCGGCGCAGCCGGTAAACACGTATTACCATGGCAGGTTGC
ACAAAAAAACAGATTATCCGGTATGCTGCGGCATAGCAGGAAGGAGGCGGAGCCGGTGAAGGTGGAGACAACGGTGCGGG
CATGGCCTGTGCAGGGCGAGTTTGCCATTTCCCGCGGCAGCAGAACAGAAGCCGTGGTGGTTGAGGTGCGCATATACGGC
GAAGGAGCGCAGGGTCGCGGCGAGTGCGTGCCCTATGCCCGTTACGGAGAAAGTGTTGAGGGCGTTTGCGCAGCTGTGCA
CCAAGCCGTGCGCGGAATGGGCGGGCGGCCGGACAGACAGCTTTTGCAGACCTTGCTGCCTGCCGGTGCGGCGCGTAACG
GGCTGGATTGTGCTCTATGGGAGTACGAGGCCGCACTGCGCGGCGAGCGGGTGTGGCAGCTGGCAGGCTGTATGCGGCCC
GTGCCCGTGCATACCGCCTATACGCTCAGTCTGGGTACGCCTCTGGCCATGGGCGCCGCCGCGCGGGAAAATGCGCACAG
ACCGCTTTTGAAGGTCAAGCTGGGCGGCGCAGGCGATATGGAGCGAATCCGCGCTGTGCACGAAAATGCCCCTGAAGCCC
GCCTGATTATTGATGCCAATGAGGCATGGACAGTACAGCAGTATGTGCAGCGGGCTCCGCAGCTGGCGTGTCTGGGCGTG
GCTCTTGTGGAGCAGCCGCTGCCTGCGGGGGCGGATGCCGGACTGGCCGGGCTGGACCGTCCCGTGCCGGTATGCGCCGA
TGAATCCTGTCACGACAGAGCAACATTGCCGCGTCTTGCCGGTCTGTACGATGCGGTGAACATCAAGCTGGACAAGACCG
GTGGCCTGACGGAAGCGCTGGCCATGCGCGATGAGGCTGTACGCATGGGCTTCAGAGTGATGGTAGGGTGCATGCTGGGG
TCGTCCCTTGCCATGGCACCGGCCCATCTTGTGGCACAGGGTGCTGATTTTGCAGATATTGACGGTCCCCTGCTGCTGGC
TGCCGATCATGATCCGGCGCTGTGCTACGAGGGCAGTCTGGTGTATCCTCCGGAGCCTGCCCTGTGGGGATAG

Upstream 100 bases:

>100_bases
TTCAGCATGCCGTGGTATTGGCTGTGGCGGGCGCATGGTTACTTGCTCCGGCGCTGATTTTCCACAGCTTATTATTTTTT
CAGAAAGTATAGGATATTGA

Downstream 100 bases:

>100_bases
GGCGCAGCGCGGCGCCGCGGCGGACGCGCGCCATGACAAATGTCCGGACGGCGGACAAAAAGTTCACGTAATGACGTCGA
AAGTGTTCACACAAGTGCGG

Product: L-alanine-DL-glutamate epimerase/enolase superfamily-like protein

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MGNGKKKLRASVPKVGGGYLGRRVSRSWSEGSVTGGTAQRRPAGRIKQVLCRQGNGRCIQGDKLLLRHLAPAKNVNMSDM
MTVYPTPAAVVWHCGAAGKHVLPWQVAQKNRLSGMLRHSRKEAEPVKVETTVRAWPVQGEFAISRGSRTEAVVVEVRIYG
EGAQGRGECVPYARYGESVEGVCAAVHQAVRGMGGRPDRQLLQTLLPAGAARNGLDCALWEYEAALRGERVWQLAGCMRP
VPVHTAYTLSLGTPLAMGAAARENAHRPLLKVKLGGAGDMERIRAVHENAPEARLIIDANEAWTVQQYVQRAPQLACLGV
ALVEQPLPAGADAGLAGLDRPVPVCADESCHDRATLPRLAGLYDAVNIKLDKTGGLTEALAMRDEAVRMGFRVMVGCMLG
SSLAMAPAHLVAQGADFADIDGPLLLAADHDPALCYEGSLVYPPEPALWG

Sequences:

>Translated_450_residues
MGNGKKKLRASVPKVGGGYLGRRVSRSWSEGSVTGGTAQRRPAGRIKQVLCRQGNGRCIQGDKLLLRHLAPAKNVNMSDM
MTVYPTPAAVVWHCGAAGKHVLPWQVAQKNRLSGMLRHSRKEAEPVKVETTVRAWPVQGEFAISRGSRTEAVVVEVRIYG
EGAQGRGECVPYARYGESVEGVCAAVHQAVRGMGGRPDRQLLQTLLPAGAARNGLDCALWEYEAALRGERVWQLAGCMRP
VPVHTAYTLSLGTPLAMGAAARENAHRPLLKVKLGGAGDMERIRAVHENAPEARLIIDANEAWTVQQYVQRAPQLACLGV
ALVEQPLPAGADAGLAGLDRPVPVCADESCHDRATLPRLAGLYDAVNIKLDKTGGLTEALAMRDEAVRMGFRVMVGCMLG
SSLAMAPAHLVAQGADFADIDGPLLLAADHDPALCYEGSLVYPPEPALWG
>Mature_449_residues
GNGKKKLRASVPKVGGGYLGRRVSRSWSEGSVTGGTAQRRPAGRIKQVLCRQGNGRCIQGDKLLLRHLAPAKNVNMSDMM
TVYPTPAAVVWHCGAAGKHVLPWQVAQKNRLSGMLRHSRKEAEPVKVETTVRAWPVQGEFAISRGSRTEAVVVEVRIYGE
GAQGRGECVPYARYGESVEGVCAAVHQAVRGMGGRPDRQLLQTLLPAGAARNGLDCALWEYEAALRGERVWQLAGCMRPV
PVHTAYTLSLGTPLAMGAAARENAHRPLLKVKLGGAGDMERIRAVHENAPEARLIIDANEAWTVQQYVQRAPQLACLGVA
LVEQPLPAGADAGLAGLDRPVPVCADESCHDRATLPRLAGLYDAVNIKLDKTGGLTEALAMRDEAVRMGFRVMVGCMLGS
SLAMAPAHLVAQGADFADIDGPLLLAADHDPALCYEGSLVYPPEPALWG

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has a role in the recycling of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of all the L-Ala-X dipeptides, excep

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=311, Percent_Identity=48.2315112540193, Blast_Score=258, Evalue=6e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 48109; Mature: 47978

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNGKKKLRASVPKVGGGYLGRRVSRSWSEGSVTGGTAQRRPAGRIKQVLCRQGNGRCIQ
CCCCHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEC
GDKLLLRHLAPAKNVNMSDMMTVYPTPAAVVWHCGAAGKHVLPWQVAQKNRLSGMLRHSR
CHHHHHHHCCCCCCCCHHHEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
KEAEPVKVETTVRAWPVQGEFAISRGSRTEAVVVEVRIYGEGAQGRGECVPYARYGESVE
CCCCCEEEEEEEEEECCCCCEEECCCCCCEEEEEEEEEEECCCCCCCCCCCHHHCCCCHH
GVCAAVHQAVRGMGGRPDRQLLQTLLPAGAARNGLDCALWEYEAALRGERVWQLAGCMRP
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCEEEHHHHHHHHCHHHHHHHHHCCCC
VPVHTAYTLSLGTPLAMGAAARENAHRPLLKVKLGGAGDMERIRAVHENAPEARLIIDAN
CCCCEEEEEECCCCHHHCHHHHHCCCCCEEEEEECCCCCHHHHHHHHCCCCCEEEEEECC
EAWTVQQYVQRAPQLACLGVALVEQPLPAGADAGLAGLDRPVPVCADESCHDRATLPRLA
CCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
GLYDAVNIKLDKTGGLTEALAMRDEAVRMGFRVMVGCMLGSSLAMAPAHLVAQGADFADI
HHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCC
DGPLLLAADHDPALCYEGSLVYPPEPALWG
CCCEEEEECCCCCEEECCCEECCCCCCCCC
>Mature Secondary Structure 
GNGKKKLRASVPKVGGGYLGRRVSRSWSEGSVTGGTAQRRPAGRIKQVLCRQGNGRCIQ
CCCHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEC
GDKLLLRHLAPAKNVNMSDMMTVYPTPAAVVWHCGAAGKHVLPWQVAQKNRLSGMLRHSR
CHHHHHHHCCCCCCCCHHHEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
KEAEPVKVETTVRAWPVQGEFAISRGSRTEAVVVEVRIYGEGAQGRGECVPYARYGESVE
CCCCCEEEEEEEEEECCCCCEEECCCCCCEEEEEEEEEEECCCCCCCCCCCHHHCCCCHH
GVCAAVHQAVRGMGGRPDRQLLQTLLPAGAARNGLDCALWEYEAALRGERVWQLAGCMRP
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCEEEHHHHHHHHCHHHHHHHHHCCCC
VPVHTAYTLSLGTPLAMGAAARENAHRPLLKVKLGGAGDMERIRAVHENAPEARLIIDAN
CCCCEEEEEECCCCHHHCHHHHHCCCCCEEEEEECCCCCHHHHHHHHCCCCCEEEEEECC
EAWTVQQYVQRAPQLACLGVALVEQPLPAGADAGLAGLDRPVPVCADESCHDRATLPRLA
CCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
GLYDAVNIKLDKTGGLTEALAMRDEAVRMGFRVMVGCMLGSSLAMAPAHLVAQGADFADI
HHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCC
DGPLLLAADHDPALCYEGSLVYPPEPALWG
CCCEEEEECCCCCEEECCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7499381; 9097039; 9278503 [H]