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The map label for this gene is ptsI [H]

Identifier: 78356136

GI number: 78356136

Start: 1119447

End: 1121225

Strand: Reverse

Name: ptsI [H]

Synonym: Dde_1089

Alternate gene names: 78356136

Gene position: 1121225-1119447 (Counterclockwise)

Preceding gene: 78356137

Following gene: 78356135

Centisome position: 30.06

GC content: 58.07

Gene sequence:

>1779_bases
TTGGCCCGTCTCATTCTTCACGGTATTCCGGTTTCCGCCGGCATATCCATAGGCAAAGCCTTTTTTGCATGGCGCGACAA
CATCAGCACGTTGCCGCGCGGCATTGTGACGCCCGGTGCGGCACAAAGCGAAGTGGAAAGGCTTGAATCCGCCGTGGAAG
CCGTGCAGAACGAGCTCACACAGGCACGCAACAGGGTGCCCGCCGAACTCAAAGACCATGCGGCCATCATTGATTCGCAC
CTGCTCATTGCCGCCGACCCCAAGCTTATAAAAGACGCAGCCAGACGCATTCAGGAGCAGAACATCACTGCCGAATGGGC
TCTGGAACAGGCCGTCGAAGCCATCGCTCTGGCGTTCAGCAGCATCGAGGATGACTATATCCGCGAGCGGGTGCAGGACG
TGCGTGTGGTGGCCGACCGCATCAGCAAACGGCTCATGGGTGCTTCCGGCTGTCTCAAGCGTCCGCTGCAGGAGCGCATG
GTGCTTATGGCGCACGATCTTGCCCCCGCGGATACCATGGAACTGCCGCTGGACAAAATCATGTCGTTTGCCACAAGCGA
AGGCGGCAAAACGAGCCACACGGGCATTCTGGCCCGCAGCATGCTCATTCCCGCCGTGGTCGGCGTCAGTAATCTGGAAG
AACACGTCAATGACGGAGATCTGGTCATCGTTGACGCACTGCGCGGCTTCATCATCGTCGATCCCAATGAAGACGAGCTG
GCAGACTATACCGAACTCAAGTTCCAGTTTGAAGCCTACCAGAAAGCCCTGCACAAGGAATGCCGCCTGCCTGCGGAAAC
AGTGGACGGCTACCGCGTGGAAGTGGTCGCCAACATTGAGATGACCGAGGAAGTGGCACAGGTGCTGGACAGTGGCGGCG
AAGGCGTGGGCCTGTACCGCACCGAATACGCATACCTCAGCCGCCCCGCGCTGCCCACGGAAGACCAGCTGTACGAAGAA
TACTCCGAGCTGGCGTCCATAATGGCACCGGGCAAGGTTATTTTCCGCACGCTGGATGTGGGCGCGGACAAAATGCTCAC
CGAGCAGACCCTCATGCGTGAACCCAATCCGGCACTGGGGCTGAGGGCCATTCGCTACTGCCTGCGCAATATCGATGTTT
TCAAAACACAGCTGAGGGCCATTCTGCGGGCCAGTGTGCACGGCAACGTGGCGCTGATGTTTCCCATGATTTCGGGCCTG
CAGGAGCTGCGGCAGGCCAAGGTGCTGCTCAACGAAGTGCGCATGGAACTGGACCGCGCGCGCCAGCCGTATAATCCCAA
CATGCCCGTGGGCATTATGATAGAGCTGCCCTCGGCCGTTCTCATCGCCGATTCACTGGCGCAGGAAGTGGATTTTTTCA
GCATCGGCACCAACGACCTTATTCAGTACTCGCTGGGCATAGACAGGGGCAACAAGCATGTCTCGTACCTGTACCAGCCG
CTGCATCCGGCCATTGTACGGTCCATCAAGTTTGTGGTAGACGCGGCTCACAGAGAAGGCATCGAGGTTTCCGTATGCGG
CGAAGTGGCGGCAGACCCCTACTGCATACCCATTCTGCTGGGCATGCAGATTGACGCCATTTCCATCGCCCCGCAGTCTA
TCCCCGGCATCAAGCGCATTATCCGCCGCACCAACATGGAAGAGTGCAAGACATTGCTCAACGAAGTGCTGCGCACCGCC
ACTGTGGCCCGCATCAACCGCGTGGTCAAAGACACAATATTCAAACAGTTTCCGGAAGAGCTCACCTTCTATTCGTCACT
TATCGATCATGACGACTAA

Upstream 100 bases:

>100_bases
AGGGACTGCGCTGGAGCTGCGAGCCAGCGGACCCGATGCGCGCGAAGCTCTGCGGCATCTTGCCGCAACATTTGCCAACC
GCTTCCGCGAGGACACGTAA

Downstream 100 bases:

>100_bases
CGGAAATTGCAGATACCGGAACACACTATGACGAAAAAAAAATCATCGGGCGGCGCTCTCATTGCCCAGAATAAAAAAGC
GCGCCATCTTTACGAGCTGC

Product: phosphoenolpyruvate--protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 592; Mature: 591

Protein sequence:

>592_residues
MARLILHGIPVSAGISIGKAFFAWRDNISTLPRGIVTPGAAQSEVERLESAVEAVQNELTQARNRVPAELKDHAAIIDSH
LLIAADPKLIKDAARRIQEQNITAEWALEQAVEAIALAFSSIEDDYIRERVQDVRVVADRISKRLMGASGCLKRPLQERM
VLMAHDLAPADTMELPLDKIMSFATSEGGKTSHTGILARSMLIPAVVGVSNLEEHVNDGDLVIVDALRGFIIVDPNEDEL
ADYTELKFQFEAYQKALHKECRLPAETVDGYRVEVVANIEMTEEVAQVLDSGGEGVGLYRTEYAYLSRPALPTEDQLYEE
YSELASIMAPGKVIFRTLDVGADKMLTEQTLMREPNPALGLRAIRYCLRNIDVFKTQLRAILRASVHGNVALMFPMISGL
QELRQAKVLLNEVRMELDRARQPYNPNMPVGIMIELPSAVLIADSLAQEVDFFSIGTNDLIQYSLGIDRGNKHVSYLYQP
LHPAIVRSIKFVVDAAHREGIEVSVCGEVAADPYCIPILLGMQIDAISIAPQSIPGIKRIIRRTNMEECKTLLNEVLRTA
TVARINRVVKDTIFKQFPEELTFYSSLIDHDD

Sequences:

>Translated_592_residues
MARLILHGIPVSAGISIGKAFFAWRDNISTLPRGIVTPGAAQSEVERLESAVEAVQNELTQARNRVPAELKDHAAIIDSH
LLIAADPKLIKDAARRIQEQNITAEWALEQAVEAIALAFSSIEDDYIRERVQDVRVVADRISKRLMGASGCLKRPLQERM
VLMAHDLAPADTMELPLDKIMSFATSEGGKTSHTGILARSMLIPAVVGVSNLEEHVNDGDLVIVDALRGFIIVDPNEDEL
ADYTELKFQFEAYQKALHKECRLPAETVDGYRVEVVANIEMTEEVAQVLDSGGEGVGLYRTEYAYLSRPALPTEDQLYEE
YSELASIMAPGKVIFRTLDVGADKMLTEQTLMREPNPALGLRAIRYCLRNIDVFKTQLRAILRASVHGNVALMFPMISGL
QELRQAKVLLNEVRMELDRARQPYNPNMPVGIMIELPSAVLIADSLAQEVDFFSIGTNDLIQYSLGIDRGNKHVSYLYQP
LHPAIVRSIKFVVDAAHREGIEVSVCGEVAADPYCIPILLGMQIDAISIAPQSIPGIKRIIRRTNMEECKTLLNEVLRTA
TVARINRVVKDTIFKQFPEELTFYSSLIDHDD
>Mature_591_residues
ARLILHGIPVSAGISIGKAFFAWRDNISTLPRGIVTPGAAQSEVERLESAVEAVQNELTQARNRVPAELKDHAAIIDSHL
LIAADPKLIKDAARRIQEQNITAEWALEQAVEAIALAFSSIEDDYIRERVQDVRVVADRISKRLMGASGCLKRPLQERMV
LMAHDLAPADTMELPLDKIMSFATSEGGKTSHTGILARSMLIPAVVGVSNLEEHVNDGDLVIVDALRGFIIVDPNEDELA
DYTELKFQFEAYQKALHKECRLPAETVDGYRVEVVANIEMTEEVAQVLDSGGEGVGLYRTEYAYLSRPALPTEDQLYEEY
SELASIMAPGKVIFRTLDVGADKMLTEQTLMREPNPALGLRAIRYCLRNIDVFKTQLRAILRASVHGNVALMFPMISGLQ
ELRQAKVLLNEVRMELDRARQPYNPNMPVGIMIELPSAVLIADSLAQEVDFFSIGTNDLIQYSLGIDRGNKHVSYLYQPL
HPAIVRSIKFVVDAAHREGIEVSVCGEVAADPYCIPILLGMQIDAISIAPQSIPGIKRIIRRTNMEECKTLLNEVLRTAT
VARINRVVKDTIFKQFPEELTFYSSLIDHDD

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=580, Percent_Identity=37.0689655172414, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1789193, Length=564, Percent_Identity=33.1560283687943, Blast_Score=294, Evalue=1e-80,
Organism=Escherichia coli, GI48994992, Length=530, Percent_Identity=34.5283018867924, Blast_Score=284, Evalue=1e-77,
Organism=Escherichia coli, GI1788726, Length=597, Percent_Identity=31.3232830820771, Blast_Score=271, Evalue=1e-73,
Organism=Escherichia coli, GI1787994, Length=400, Percent_Identity=27, Blast_Score=109, Evalue=4e-25,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 65791; Mature: 65659

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLILHGIPVSAGISIGKAFFAWRDNISTLPRGIVTPGAAQSEVERLESAVEAVQNELT
CCCEEEECCCCCCCCHHHHHHHHHHCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
QARNRVPAELKDHAAIIDSHLLIAADPKLIKDAARRIQEQNITAEWALEQAVEAIALAFS
HHHHCCCCHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
SIEDDYIRERVQDVRVVADRISKRLMGASGCLKRPLQERMVLMAHDLAPADTMELPLDKI
HCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHCCCCCCHHHCCHHHH
MSFATSEGGKTSHTGILARSMLIPAVVGVSNLEEHVNDGDLVIVDALRGFIIVDPNEDEL
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCHHH
ADYTELKFQFEAYQKALHKECRLPAETVDGYRVEVVANIEMTEEVAQVLDSGGEGVGLYR
HHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEEEECCHHHHHHHHHHHCCCCCCCEEE
TEYAYLSRPALPTEDQLYEEYSELASIMAPGKVIFRTLDVGADKMLTEQTLMREPNPALG
HHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHCCCCCHHH
LRAIRYCLRNIDVFKTQLRAILRASVHGNVALMFPMISGLQELRQAKVLLNEVRMELDRA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RQPYNPNMPVGIMIELPSAVLIADSLAQEVDFFSIGTNDLIQYSLGIDRGNKHVSYLYQP
CCCCCCCCCEEEEEECCCHHHHHHHHHHHCCEEECCCHHHHHHCCCCCCCCCHHHHHHHH
LHPAIVRSIKFVVDAAHREGIEVSVCGEVAADPYCIPILLGMQIDAISIAPQSIPGIKRI
CCHHHHHHHHHHHHHHHHCCCEEEECCHHCCCCCHHHHHCCCEEEEEECCCCCCHHHHHH
IRRTNMEECKTLLNEVLRTATVARINRVVKDTIFKQFPEELTFYSSLIDHDD
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ARLILHGIPVSAGISIGKAFFAWRDNISTLPRGIVTPGAAQSEVERLESAVEAVQNELT
CCEEEECCCCCCCCHHHHHHHHHHCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
QARNRVPAELKDHAAIIDSHLLIAADPKLIKDAARRIQEQNITAEWALEQAVEAIALAFS
HHHHCCCCHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
SIEDDYIRERVQDVRVVADRISKRLMGASGCLKRPLQERMVLMAHDLAPADTMELPLDKI
HCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHCCCCCCHHHCCHHHH
MSFATSEGGKTSHTGILARSMLIPAVVGVSNLEEHVNDGDLVIVDALRGFIIVDPNEDEL
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCHHH
ADYTELKFQFEAYQKALHKECRLPAETVDGYRVEVVANIEMTEEVAQVLDSGGEGVGLYR
HHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEEEECCHHHHHHHHHHHCCCCCCCEEE
TEYAYLSRPALPTEDQLYEEYSELASIMAPGKVIFRTLDVGADKMLTEQTLMREPNPALG
HHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHCCCCCHHH
LRAIRYCLRNIDVFKTQLRAILRASVHGNVALMFPMISGLQELRQAKVLLNEVRMELDRA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RQPYNPNMPVGIMIELPSAVLIADSLAQEVDFFSIGTNDLIQYSLGIDRGNKHVSYLYQP
CCCCCCCCCEEEEEECCCHHHHHHHHHHHCCEEECCCHHHHHHCCCCCCCCCHHHHHHHH
LHPAIVRSIKFVVDAAHREGIEVSVCGEVAADPYCIPILLGMQIDAISIAPQSIPGIKRI
CCHHHHHHHHHHHHHHHHCCCEEEECCHHCCCCCHHHHHCCCEEEEEECCCCCCHHHHHH
IRRTNMEECKTLLNEVLRTATVARINRVVKDTIFKQFPEELTFYSSLIDHDD
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]