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The map label for this gene is glcD [H]

Identifier: 78356134

GI number: 78356134

Start: 1117424

End: 1118839

Strand: Reverse

Name: glcD [H]

Synonym: Dde_1087

Alternate gene names: 78356134

Gene position: 1118839-1117424 (Counterclockwise)

Preceding gene: 78356135

Following gene: 78356133

Centisome position: 29.99

GC content: 61.79

Gene sequence:

>1416_bases
ATGACACGCACCACGCACCAGCACCAACGTTTTCTCAGCGACCTTTTTCCCCAAGGCGACTGCCTGTTCCGCCCCGAGGA
ACTGCTTGTTTTCGGCATGGACGCCAGCAGGCTGGATGCCCGCCCCATGGCGGTTGTCAGACCGCGTGAAGCGCGACAGA
TAGCCGAACTGCTGCAATATGCACAGACAGAACGCATTCCCGTGTATCCGAGAGCACGCGCCACAAACGTGGTGGGCGCC
TGTGTTCCCGCCGACCCGCGCCGCACCGGTACGCAGGAACCACAGGGCATTGTGGTGTCCACCCTGCTTATGGACAGCAT
CATCGATATCTCGCCTGACGACTTTGTAGCCGTGGTGCAGCCCGGCGTCATCACCCGCGACCTTCAGCAGAATGTGGAAC
GGCAGGGGCTGTTTTATCCGCCGGATCCCGCCAGTCTGAACATTTCCACCATCGGGGGCAACGTCGCCACCTGTGCGGGC
GGCATGCGCGCCCTCAAATACGGCGTAACCCGCGAATATGTACTGGGCATGGAAGCAGCCCTGCCGGGCGGACGCATGCT
GCGCACAGGCGGGCGGTGTCATAAAAACGTAGTGGGCCTCGACCTGGTGCGCCTGATGACCGGCAGCGAAGGGACGCTGG
GTATCATGACCGAAATAACCCTGAAGCTGCTGCCCCTGCCCGAGGCCACTGCGTCGCTGCTGGCCGGTTTTTCCGGCATG
GAACAGGCGGTACAGGCTATACGCAATGTGTTCCGCGCCGGCATGCTGCCCGCCGCGCTGGAATTCATGGGGCCGGAAAC
GCTGGACTGTCTGGCTTTGCGGCGCACGCCCCCATGGCCCGGCAATGTCCGTGCCGCCCTGCTTTTCCGGCTGGACGGCA
CCAGCGGAGCTTTGAAAGCGGAACGCCGCAGACTGGAATCAGTCCTGCAGTCCGACGCGGATTGCACACCCGTCTGGCAG
GCCGCAGGCAGCGGCCCGCAGGAAGAAGAACCCCTGTGGGAAATACGCCGCGGCATCAATCCCGCATCGTACCTTGCGGC
GCCGGACAAAATCAGCGACGATGTGACCGTGCCCCGGGGCATGCTGCTGCCCGCCCTCACAGACATTGCGGCCACAGGCA
GAAAGCACGGCCTGACCATACTTACATTCGGTCATGTGGGTGACGGCAACATCCATGTGAACATCATGCACGACAAATCC
CTGCCCGGCGTGCGGGAAAAAGCAGTGGCAGCCAAAAAAGAAATTATGAATACCGTGCTTTCCATGGGCGGCACCCTGTC
CGGCGAACACGGCGTGGGGCTTACAAAGGCCCCGTACATCGACAACCAGCTGTCCCCTGTGGAACGGCAACTGATGGCGG
GCATAAAGGCATCCTTTGATCCCGAAGGCATCATGAATCCCGGCAAAGCCTACTGA

Upstream 100 bases:

>100_bases
CACCGCGCGTCAGGTGCGGTGTTTCCTTTTTTCCGCGCCGGTGCAGCCACCGGCGTAACCTCTTGCCGCCCCATGCCAGC
CTGCGCGCCGGACCATCCAG

Downstream 100 bases:

>100_bases
CCCCCGCGGGCAGACAGGCACCGGCACCACTCTGGAGCAGCCATGACAGCCGTATGGAGCATTTTGCGCCGCAGCGTTAT
ACCGCTTGCCTTCACCGCAG

Product: glycolate oxidase subunit GlcD

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MTRTTHQHQRFLSDLFPQGDCLFRPEELLVFGMDASRLDARPMAVVRPREARQIAELLQYAQTERIPVYPRARATNVVGA
CVPADPRRTGTQEPQGIVVSTLLMDSIIDISPDDFVAVVQPGVITRDLQQNVERQGLFYPPDPASLNISTIGGNVATCAG
GMRALKYGVTREYVLGMEAALPGGRMLRTGGRCHKNVVGLDLVRLMTGSEGTLGIMTEITLKLLPLPEATASLLAGFSGM
EQAVQAIRNVFRAGMLPAALEFMGPETLDCLALRRTPPWPGNVRAALLFRLDGTSGALKAERRRLESVLQSDADCTPVWQ
AAGSGPQEEEPLWEIRRGINPASYLAAPDKISDDVTVPRGMLLPALTDIAATGRKHGLTILTFGHVGDGNIHVNIMHDKS
LPGVREKAVAAKKEIMNTVLSMGGTLSGEHGVGLTKAPYIDNQLSPVERQLMAGIKASFDPEGIMNPGKAY

Sequences:

>Translated_471_residues
MTRTTHQHQRFLSDLFPQGDCLFRPEELLVFGMDASRLDARPMAVVRPREARQIAELLQYAQTERIPVYPRARATNVVGA
CVPADPRRTGTQEPQGIVVSTLLMDSIIDISPDDFVAVVQPGVITRDLQQNVERQGLFYPPDPASLNISTIGGNVATCAG
GMRALKYGVTREYVLGMEAALPGGRMLRTGGRCHKNVVGLDLVRLMTGSEGTLGIMTEITLKLLPLPEATASLLAGFSGM
EQAVQAIRNVFRAGMLPAALEFMGPETLDCLALRRTPPWPGNVRAALLFRLDGTSGALKAERRRLESVLQSDADCTPVWQ
AAGSGPQEEEPLWEIRRGINPASYLAAPDKISDDVTVPRGMLLPALTDIAATGRKHGLTILTFGHVGDGNIHVNIMHDKS
LPGVREKAVAAKKEIMNTVLSMGGTLSGEHGVGLTKAPYIDNQLSPVERQLMAGIKASFDPEGIMNPGKAY
>Mature_470_residues
TRTTHQHQRFLSDLFPQGDCLFRPEELLVFGMDASRLDARPMAVVRPREARQIAELLQYAQTERIPVYPRARATNVVGAC
VPADPRRTGTQEPQGIVVSTLLMDSIIDISPDDFVAVVQPGVITRDLQQNVERQGLFYPPDPASLNISTIGGNVATCAGG
MRALKYGVTREYVLGMEAALPGGRMLRTGGRCHKNVVGLDLVRLMTGSEGTLGIMTEITLKLLPLPEATASLLAGFSGME
QAVQAIRNVFRAGMLPAALEFMGPETLDCLALRRTPPWPGNVRAALLFRLDGTSGALKAERRRLESVLQSDADCTPVWQA
AGSGPQEEEPLWEIRRGINPASYLAAPDKISDDVTVPRGMLLPALTDIAATGRKHGLTILTFGHVGDGNIHVNIMHDKSL
PGVREKAVAAKKEIMNTVLSMGGTLSGEHGVGLTKAPYIDNQLSPVERQLMAGIKASFDPEGIMNPGKAY

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=433, Percent_Identity=30.715935334873, Blast_Score=202, Evalue=7e-52,
Organism=Homo sapiens, GI37595754, Length=456, Percent_Identity=29.3859649122807, Blast_Score=193, Evalue=3e-49,
Organism=Homo sapiens, GI119964728, Length=458, Percent_Identity=28.82096069869, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI4501993, Length=249, Percent_Identity=27.3092369477912, Blast_Score=96, Evalue=9e-20,
Organism=Escherichia coli, GI1789351, Length=464, Percent_Identity=33.4051724137931, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1787977, Length=541, Percent_Identity=26.0628465804067, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI48994914, Length=200, Percent_Identity=33, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI48994907, Length=224, Percent_Identity=27.2321428571429, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71992373, Length=466, Percent_Identity=29.1845493562232, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI71992381, Length=466, Percent_Identity=28.9699570815451, Blast_Score=169, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI71992392, Length=400, Percent_Identity=29.5, Blast_Score=157, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI17534361, Length=447, Percent_Identity=27.0693512304251, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17556096, Length=222, Percent_Identity=27.4774774774775, Blast_Score=98, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6320764, Length=463, Percent_Identity=25.4859611231102, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6320023, Length=467, Percent_Identity=27.4089935760171, Blast_Score=157, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6320027, Length=443, Percent_Identity=27.765237020316, Blast_Score=153, Evalue=6e-38,
Organism=Drosophila melanogaster, GI18921117, Length=443, Percent_Identity=24.3792325056433, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24639277, Length=443, Percent_Identity=24.3792325056433, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24639275, Length=443, Percent_Identity=24.3792325056433, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24653753, Length=483, Percent_Identity=24.223602484472, Blast_Score=102, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 50854; Mature: 50723

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: PS00626 RCC1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTTHQHQRFLSDLFPQGDCLFRPEELLVFGMDASRLDARPMAVVRPREARQIAELLQY
CCCCCHHHHHHHHHHCCCCCEEECCCCEEEECCCHHHCCCCCEEEECCHHHHHHHHHHHH
AQTERIPVYPRARATNVVGACVPADPRRTGTQEPQGIVVSTLLMDSIIDISPDDFVAVVQ
HHCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEC
PGVITRDLQQNVERQGLFYPPDPASLNISTIGGNVATCAGGMRALKYGVTREYVLGMEAA
CCCCHHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHC
LPGGRMLRTGGRCHKNVVGLDLVRLMTGSEGTLGIMTEITLKLLPLPEATASLLAGFSGM
CCCCCEECCCCCHHHHHHHHHHHHHHCCCCCCEEEHEEEEEEEECCCHHHHHHHHHHHHH
EQAVQAIRNVFRAGMLPAALEFMGPETLDCLALRRTPPWPGNVRAALLFRLDGTSGALKA
HHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHEECCCCCCCCCCEEEEEEEECCCCCHHHH
ERRRLESVLQSDADCTPVWQAAGSGPQEEEPLWEIRRGINPASYLAAPDKISDDVTVPRG
HHHHHHHHHHCCCCCCHHHHHCCCCCCCCCHHHHHHHCCCHHHHHCCCCCCCCCCCCCCC
MLLPALTDIAATGRKHGLTILTFGHVGDGNIHVNIMHDKSLPGVREKAVAAKKEIMNTVL
HHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
SMGGTLSGEHGVGLTKAPYIDNQLSPVERQLMAGIKASFDPEGIMNPGKAY
HHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TRTTHQHQRFLSDLFPQGDCLFRPEELLVFGMDASRLDARPMAVVRPREARQIAELLQY
CCCCHHHHHHHHHHCCCCCEEECCCCEEEECCCHHHCCCCCEEEECCHHHHHHHHHHHH
AQTERIPVYPRARATNVVGACVPADPRRTGTQEPQGIVVSTLLMDSIIDISPDDFVAVVQ
HHCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEC
PGVITRDLQQNVERQGLFYPPDPASLNISTIGGNVATCAGGMRALKYGVTREYVLGMEAA
CCCCHHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHC
LPGGRMLRTGGRCHKNVVGLDLVRLMTGSEGTLGIMTEITLKLLPLPEATASLLAGFSGM
CCCCCEECCCCCHHHHHHHHHHHHHHCCCCCCEEEHEEEEEEEECCCHHHHHHHHHHHHH
EQAVQAIRNVFRAGMLPAALEFMGPETLDCLALRRTPPWPGNVRAALLFRLDGTSGALKA
HHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHEECCCCCCCCCCEEEEEEEECCCCCHHHH
ERRRLESVLQSDADCTPVWQAAGSGPQEEEPLWEIRRGINPASYLAAPDKISDDVTVPRG
HHHHHHHHHHCCCCCCHHHHHCCCCCCCCCHHHHHHHCCCHHHHHCCCCCCCCCCCCCCC
MLLPALTDIAATGRKHGLTILTFGHVGDGNIHVNIMHDKSLPGVREKAVAAKKEIMNTVL
HHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
SMGGTLSGEHGVGLTKAPYIDNQLSPVERQLMAGIKASFDPEGIMNPGKAY
HHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]