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The map label for this gene is ogt [H]

Identifier: 78356045

GI number: 78356045

Start: 1023661

End: 1024287

Strand: Direct

Name: ogt [H]

Synonym: Dde_0998

Alternate gene names: 78356045

Gene position: 1023661-1024287 (Clockwise)

Preceding gene: 78356044

Following gene: 78356047

Centisome position: 27.44

GC content: 65.71

Gene sequence:

>627_bases
ATGTACGATGTCGAGATACTGGTGAGCGGCCCGCTGGCCATGCTGCTTGAATGGAGCGGCCCTGATGGCGCGCCGGACGG
ACCCGCCTGTGCGGAGCCCTGTATTGCCCGTGTGGGGCTGCGCTGGTCGGCAGGACTTGACGGAAGCGGTCTGCTTACGG
GGGTAAAACTGCCCCGGCCCGGCGCGGAAGCCGGCGACCTGCTGCCCCGTACTCTCGTGGCATTTTCCGGCAGCGTGCCG
GACGGCATGCATAACGGTGCGGCGGAACTTTCGGCCACCGGCAGCCGCATGCTTGAGGCGCTGGACAGTTATGTACAGCA
GGGCAGAACGCAGTGGCCACAGGTTGCTCTGCCCATGGACGGACTTACCCCGTTTGCCCGCAAGGTGCTGGAAACGCTGC
GGCACGAGGTACCCGCCGGCAGTACCGTCACATACGGGCAACTGGCCGCCATGGCCGGCCGCCCTGCAGCCGCCCGCGGG
GTGGGGCAGATAATGGCCCGCAACCGCTGGCCGCTTGTCATTCCCTGCCACAGGGTTCTGGGCAGCAAAGGGGCCATGGT
GGGGTATACGGGTGCGGGCGTAGATATGAAACGCTATCTGCTGCAGCTGGAAGGGGCTTCACTGTAA

Upstream 100 bases:

>100_bases
CTTTGCGACGGCCGCTGCTGACAGCGGCGCTGTGCCTTACGGTACAAGCCGCAAAGCGAACCTGATTTGTGTGCAACCGT
TAACGCGACGGGGGTAGGCT

Downstream 100 bases:

>100_bases
GCGGGCAGGCTGTCAGGCCAGAAGCAGCGCACACCACGGCGGCAGAGTGACCGGTATGTGTGTTCCCGCGGCAAAGCGGT
GGGGCAGGTCTGCCTCATCT

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MYDVEILVSGPLAMLLEWSGPDGAPDGPACAEPCIARVGLRWSAGLDGSGLLTGVKLPRPGAEAGDLLPRTLVAFSGSVP
DGMHNGAAELSATGSRMLEALDSYVQQGRTQWPQVALPMDGLTPFARKVLETLRHEVPAGSTVTYGQLAAMAGRPAAARG
VGQIMARNRWPLVIPCHRVLGSKGAMVGYTGAGVDMKRYLLQLEGASL

Sequences:

>Translated_208_residues
MYDVEILVSGPLAMLLEWSGPDGAPDGPACAEPCIARVGLRWSAGLDGSGLLTGVKLPRPGAEAGDLLPRTLVAFSGSVP
DGMHNGAAELSATGSRMLEALDSYVQQGRTQWPQVALPMDGLTPFARKVLETLRHEVPAGSTVTYGQLAAMAGRPAAARG
VGQIMARNRWPLVIPCHRVLGSKGAMVGYTGAGVDMKRYLLQLEGASL
>Mature_208_residues
MYDVEILVSGPLAMLLEWSGPDGAPDGPACAEPCIARVGLRWSAGLDGSGLLTGVKLPRPGAEAGDLLPRTLVAFSGSVP
DGMHNGAAELSATGSRMLEALDSYVQQGRTQWPQVALPMDGLTPFARKVLETLRHEVPAGSTVTYGQLAAMAGRPAAARG
VGQIMARNRWPLVIPCHRVLGSKGAMVGYTGAGVDMKRYLLQLEGASL

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=112, Percent_Identity=42.8571428571429, Blast_Score=75, Evalue=6e-14,
Organism=Escherichia coli, GI1787596, Length=88, Percent_Identity=51.1363636363636, Blast_Score=80, Evalue=7e-17,
Organism=Escherichia coli, GI1788542, Length=94, Percent_Identity=40.4255319148936, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI115533070, Length=111, Percent_Identity=37.8378378378378, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI115533068, Length=111, Percent_Identity=37.8378378378378, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320001, Length=97, Percent_Identity=39.1752577319588, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 21732; Mature: 21732

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDVEILVSGPLAMLLEWSGPDGAPDGPACAEPCIARVGLRWSAGLDGSGLLTGVKLPRP
CCEEEEEECCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCC
GAEAGDLLPRTLVAFSGSVPDGMHNGAAELSATGSRMLEALDSYVQQGRTQWPQVALPMD
CCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCC
GLTPFARKVLETLRHEVPAGSTVTYGQLAAMAGRPAAARGVGQIMARNRWPLVIPCHRVL
CCCHHHHHHHHHHHHHCCCCCEECHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEHHHHH
GSKGAMVGYTGAGVDMKRYLLQLEGASL
CCCCCEEEECCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MYDVEILVSGPLAMLLEWSGPDGAPDGPACAEPCIARVGLRWSAGLDGSGLLTGVKLPRP
CCEEEEEECCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCC
GAEAGDLLPRTLVAFSGSVPDGMHNGAAELSATGSRMLEALDSYVQQGRTQWPQVALPMD
CCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCC
GLTPFARKVLETLRHEVPAGSTVTYGQLAAMAGRPAAARGVGQIMARNRWPLVIPCHRVL
CCCHHHHHHHHHHHHHCCCCCEECHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEHHHHH
GSKGAMVGYTGAGVDMKRYLLQLEGASL
CCCCCEEEECCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]