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The map label for this gene is ntrX [H]

Identifier: 78355741

GI number: 78355741

Start: 708515

End: 708904

Strand: Reverse

Name: ntrX [H]

Synonym: Dde_0694

Alternate gene names: 78355741

Gene position: 708904-708515 (Counterclockwise)

Preceding gene: 78355742

Following gene: 78355740

Centisome position: 19.0

GC content: 59.49

Gene sequence:

>390_bases
ATGCAGGAGCCGCACATGACAGCCAATGACAGTCCCCATGTTCTGCTGGTGGACGATGAACCCGCCTTCGGCGACGTACT
GGCCCGCAGGCTGCGCCGTCGCAATATCCGGGTCACCCATGCGCTGGACGGCGACACAGCCCTGCGCATGCTGCTGAACA
ACGAGTTCAGTGCCGTGCTGCTCGACCTGAAAATGTCCGGCATCAGCGGGCTTGAAATTCTGAACGTGCTCAGCCGCATG
GTGCCCGACGTGCCGGTCATCATGATTTCCGGCCATGCCGATCAGGAAACAGTGGCGCACTGTCTGGCCAACGGCGCCTG
CCAGTATCTGCCCAAACCGTGCGATGCCGACGAAATCGTCTCCATAATAACCCGCATGCAAAAAAACTGA

Upstream 100 bases:

>100_bases
ATGCCCGCATACACCCCGAAGAGCAATCCCCGACACAGCCTGAAACCGTGCGGTGCCGACGGCACCGCGGAAAAAAAAGA
CAATGCAACAGGATATGAAA

Downstream 100 bases:

>100_bases
CCAAGGTATCAATGAGGAGTTTCACATGGAAGCAACCTTCATGTCCCCGCAGGTGCTCGCCGTCACGGCAATTCTGATTC
TGGCCCTGTACTTCCTTGTC

Product: response regulator receiver domain-containing protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 129; Mature: 129

Protein sequence:

>129_residues
MQEPHMTANDSPHVLLVDDEPAFGDVLARRLRRRNIRVTHALDGDTALRMLLNNEFSAVLLDLKMSGISGLEILNVLSRM
VPDVPVIMISGHADQETVAHCLANGACQYLPKPCDADEIVSIITRMQKN

Sequences:

>Translated_129_residues
MQEPHMTANDSPHVLLVDDEPAFGDVLARRLRRRNIRVTHALDGDTALRMLLNNEFSAVLLDLKMSGISGLEILNVLSRM
VPDVPVIMISGHADQETVAHCLANGACQYLPKPCDADEIVSIITRMQKN
>Mature_129_residues
MQEPHMTANDSPHVLLVDDEPAFGDVLARRLRRRNIRVTHALDGDTALRMLLNNEFSAVLLDLKMSGISGLEILNVLSRM
VPDVPVIMISGHADQETVAHCLANGACQYLPKPCDADEIVSIITRMQKN

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG4567

COG function: function code TK; Response regulator consisting of a CheY-like receiver domain and a Fis-type HTH domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=112, Percent_Identity=33.0357142857143, Blast_Score=67, Evalue=4e-13,
Organism=Escherichia coli, GI1790437, Length=117, Percent_Identity=29.0598290598291, Blast_Score=59, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 14257; Mature: 14257

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
7.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEPHMTANDSPHVLLVDDEPAFGDVLARRLRRRNIRVTHALDGDTALRMLLNNEFSAVL
CCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCHHHEE
LDLKMSGISGLEILNVLSRMVPDVPVIMISGHADQETVAHCLANGACQYLPKPCDADEIV
EEHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCHHCCCCCCCHHHHH
SIITRMQKN
HHHHHHHCC
>Mature Secondary Structure
MQEPHMTANDSPHVLLVDDEPAFGDVLARRLRRRNIRVTHALDGDTALRMLLNNEFSAVL
CCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCHHHEE
LDLKMSGISGLEILNVLSRMVPDVPVIMISGHADQETVAHCLANGACQYLPKPCDADEIV
EEHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCHHCCCCCCCHHHHH
SIITRMQKN
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]