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The map label for this gene is hybA [C]

Identifier: 78355699

GI number: 78355699

Start: 670158

End: 671261

Strand: Reverse

Name: hybA [C]

Synonym: Dde_0652

Alternate gene names: 78355699

Gene position: 671261-670158 (Counterclockwise)

Preceding gene: 78355700

Following gene: 78355698

Centisome position: 18.0

GC content: 59.24

Gene sequence:

>1104_bases
ATGCGTCGAAGAACCTTTCTGAGCCTGCTCGGTGCTGCCGGACTTTCGGTGGCAGCGGGCAAGCCCGCCGCCGCGGGGGG
CAATCACAGCTTTTCCGGCTACCCCGACAGCTACGGCGTTCTGCATGATTCCACCCGCTGCATCGGCTGCCGCAAATGCG
AGGAAGCGTGCCAGCAGGTGAACAATCTGCCCATGCCCGAAAAGCCGTTTGACGACCTTTCGGTGCTTGAAACCAAGCGC
AGAACAGATGACGCCACCTATACAGTGGTCAACAAATACAACGTGGCCGGCCTTGAACACCCTGTGTTCCGCAAACAGCA
GTGCAACCACTGTCTGGAACCCGCCTGCGCCTCGGCCTGCTTTGTAAAGGCGTTTACCAAAAACCCCGACGGCTCGGTTA
CATACAACCCCAAGCTGTGCGTGGGCTGCCGGTACTGCATGATCGCCTGCCCGTTCAACGTGCCCGCGTTTGAATACGGT
GAAGCATATGATCCGCTGATTACCAAGTGCACCATGTGTCACCCCCGCCTGCAGGAAGGCAAGCTGCCGGGATGCGTTGA
AAAATGCCCCAAGGAAGCTCTGACTTTCGGCAAGCGCGAAGACCTGCTGCGCATCGCGCGCGACAGAATCCGCAAAAACC
CTGAACGCTATCAGGACCACATCTACGGCGAACACGAAATGGGCGGCACCAACTGGCTGTACCTTGCCGGTGTACCCTTC
GGCGAACTGGGTCAGGATGAACATCTGGGCACCAAGCCCGCGCAGGAACATACCGCCGGCGCACTGGGTTCCGTGCCCAT
GGTTGTAGGCATCTGGCCCGTGCTGCTTACGGGTGCGTACGCCATTTCCAAACGCAAAGATAAAATAGCGCAGGAAGAAC
AGGCCGCCGCCGTACGCCATGCCGTGGGCGAAACGCAGGCCGAAGCGGACGCCAAGCTGCAGGCCGCCATGGAAAAGGCC
GCCAAGGAAAAAGAAGCCGCCATTGCCCGCGAAGTGAAAAAGGCAAAGGAAGAGGCTGAAAAAGAGTTCGAAGCGCGCCT
TGCCGCTCAAAACGAACCCGAATCCGCCGAATCCGGGACGGAAAAGCCCGAGGAGGACGCATAA

Upstream 100 bases:

>100_bases
GATGCCACACCGCCATGCGCATTGAAAAGCCTGCCAACACGGCTTGCAACGAGTGTCACAAAAAACGCGATCTGTAGGAT
ATTAGCAAGGAGACTAGTCC

Downstream 100 bases:

>100_bases
ATGGCTGACATACAGAACAACCGTAACGGGTTGCTGACACCCGGCAACATCCTGACGGCCATTATCCTTGTGGGCGGTCT
CATTCTGACGTTCATCCGCT

Product: hypothetical protein

Products: NA

Alternate protein names: HMC operon ORF 2 [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MRRRTFLSLLGAAGLSVAAGKPAAAGGNHSFSGYPDSYGVLHDSTRCIGCRKCEEACQQVNNLPMPEKPFDDLSVLETKR
RTDDATYTVVNKYNVAGLEHPVFRKQQCNHCLEPACASACFVKAFTKNPDGSVTYNPKLCVGCRYCMIACPFNVPAFEYG
EAYDPLITKCTMCHPRLQEGKLPGCVEKCPKEALTFGKREDLLRIARDRIRKNPERYQDHIYGEHEMGGTNWLYLAGVPF
GELGQDEHLGTKPAQEHTAGALGSVPMVVGIWPVLLTGAYAISKRKDKIAQEEQAAAVRHAVGETQAEADAKLQAAMEKA
AKEKEAAIAREVKKAKEEAEKEFEARLAAQNEPESAESGTEKPEEDA

Sequences:

>Translated_367_residues
MRRRTFLSLLGAAGLSVAAGKPAAAGGNHSFSGYPDSYGVLHDSTRCIGCRKCEEACQQVNNLPMPEKPFDDLSVLETKR
RTDDATYTVVNKYNVAGLEHPVFRKQQCNHCLEPACASACFVKAFTKNPDGSVTYNPKLCVGCRYCMIACPFNVPAFEYG
EAYDPLITKCTMCHPRLQEGKLPGCVEKCPKEALTFGKREDLLRIARDRIRKNPERYQDHIYGEHEMGGTNWLYLAGVPF
GELGQDEHLGTKPAQEHTAGALGSVPMVVGIWPVLLTGAYAISKRKDKIAQEEQAAAVRHAVGETQAEADAKLQAAMEKA
AKEKEAAIAREVKKAKEEAEKEFEARLAAQNEPESAESGTEKPEEDA
>Mature_367_residues
MRRRTFLSLLGAAGLSVAAGKPAAAGGNHSFSGYPDSYGVLHDSTRCIGCRKCEEACQQVNNLPMPEKPFDDLSVLETKR
RTDDATYTVVNKYNVAGLEHPVFRKQQCNHCLEPACASACFVKAFTKNPDGSVTYNPKLCVGCRYCMIACPFNVPAFEYG
EAYDPLITKCTMCHPRLQEGKLPGCVEKCPKEALTFGKREDLLRIARDRIRKNPERYQDHIYGEHEMGGTNWLYLAGVPF
GELGQDEHLGTKPAQEHTAGALGSVPMVVGIWPVLLTGAYAISKRKDKIAQEEQAAAVRHAVGETQAEADAKLQAAMEKA
AKEKEAAIAREVKKAKEEAEKEFEARLAAQNEPESAESGTEKPEEDA

Specific function: HMWC (high-molecular-weight cytochrome c precursor), ORF2, ORF3, ORF4, ORF5, ORF6 in the HMC operon form a transmembrane protein complex that allows electron flow from the periplasmic hydrogenase to the cytoplasmic enzymes that catalyze reduction of sulfa

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789370, Length=273, Percent_Identity=34.7985347985348, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI2367345, Length=247, Percent_Identity=34.412955465587, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787749, Length=200, Percent_Identity=31, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1790326, Length=294, Percent_Identity=27.2108843537415, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1787872, Length=165, Percent_Identity=36.969696969697, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1787122, Length=167, Percent_Identity=35.9281437125749, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI226510944, Length=165, Percent_Identity=32.7272727272727, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI87082180, Length=174, Percent_Identity=32.183908045977, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87082179, Length=173, Percent_Identity=30.635838150289, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI2367245, Length=175, Percent_Identity=28.5714285714286, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788811, Length=180, Percent_Identity=27.7777777777778, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 40240; Mature: 40240

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
4.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRRTFLSLLGAAGLSVAAGKPAAAGGNHSFSGYPDSYGVLHDSTRCIGCRKCEEACQQV
CCHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
NNLPMPEKPFDDLSVLETKRRTDDATYTVVNKYNVAGLEHPVFRKQQCNHCLEPACASAC
HCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FVKAFTKNPDGSVTYNPKLCVGCRYCMIACPFNVPAFEYGEAYDPLITKCTMCHPRLQEG
HHHHHHCCCCCCEEECCCHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCHHCC
KLPGCVEKCPKEALTFGKREDLLRIARDRIRKNPERYQDHIYGEHEMGGTNWLYLAGVPF
CCCCHHHHCCHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCEEEEECCCC
GELGQDEHLGTKPAQEHTAGALGSVPMVVGIWPVLLTGAYAISKRKDKIAQEEQAAAVRH
HHCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVGETQAEADAKLQAAMEKAAKEKEAAIAREVKKAKEEAEKEFEARLAAQNEPESAESGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCC
EKPEEDA
CCCCCCC
>Mature Secondary Structure
MRRRTFLSLLGAAGLSVAAGKPAAAGGNHSFSGYPDSYGVLHDSTRCIGCRKCEEACQQV
CCHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
NNLPMPEKPFDDLSVLETKRRTDDATYTVVNKYNVAGLEHPVFRKQQCNHCLEPACASAC
HCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FVKAFTKNPDGSVTYNPKLCVGCRYCMIACPFNVPAFEYGEAYDPLITKCTMCHPRLQEG
HHHHHHCCCCCCEEECCCHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCHHCC
KLPGCVEKCPKEALTFGKREDLLRIARDRIRKNPERYQDHIYGEHEMGGTNWLYLAGVPF
CCCCHHHHCCHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCEEEEECCCC
GELGQDEHLGTKPAQEHTAGALGSVPMVVGIWPVLLTGAYAISKRKDKIAQEEQAAAVRH
HHCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVGETQAEADAKLQAAMEKAAKEKEAAIAREVKKAKEEAEKEFEARLAAQNEPESAESGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCC
EKPEEDA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8335628 [H]