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The map label for this gene is glnS [H]

Identifier: 78355673

GI number: 78355673

Start: 645403

End: 647109

Strand: Reverse

Name: glnS [H]

Synonym: Dde_0626

Alternate gene names: 78355673

Gene position: 647109-645403 (Counterclockwise)

Preceding gene: 78355674

Following gene: 78355668

Centisome position: 17.35

GC content: 56.47

Gene sequence:

>1707_bases
ATGAGCATCAACCCAGCCGATACGGACAAAAAAAAGGAAGCCGGCGTCGATTTCATCCGCGCCATTGTCAACGAAGACAT
GAAGACCGGCAAATATCAGGGCCGCGTCCACACACGCTTTCCTCCCGAACCCAACGGGTACCTGCATCTGGGCCATGCAA
AATCCATCTGCCTGAATTTCGGCCTTGCTCAGGAATACGGCGGCAAGTGCAATCTGCGACTGGATGACACCAACCCGCTC
AAGGAAACCCCCGAATACGTCGAGTCCATCAAAACAGATGTGCGCTGGCTGGGCTTTGACTGGGAAGATCGCATGTTTCA
TGCCTCGGACTATTTCGAACGTCTGTTCGAACTTGCCGTACAGCTTATCAAGGCGGGCAAGGCATATGTGGATGACCTGA
CCCCCGAACAGATACGCGAATACCGCGGCACCCTGAAAGAGCCCGGCAAAAACAGCCCCTACCGCGACCGCAGCGCCGAG
GAAAACCTCGACCTGTTCATGCGTATGCGCAACGGCGAATTTGAAGACGGCAGCCGCGTACTGCGTGCCAAAATAGACAT
GGCATCGCCCAACATGGTCATGCGCGATCCCACGATCTACCGTATACGCCGTGCGCACCATCACCGCACGGGCGACGCGT
GGTGCATCTATCCCATGTACGACTTCACGCACTGCATTTCCGATTCCATCGAAGGCATCACCCATTCCATCTGCACGCTG
GAGTTTGAAAACAACCGCGAGCTGTATGACTGGATGCTGAATACGCTGGAACTCTATCATCCGCAGCAGATAGAATTTGC
CCGGCTGAATCTGACCCACACCGTGCTTTCCAAACGCAAACTTATCCAGCTGGTGGAAGAAGGTCATGTAAACGGCTGGG
ACGACCCGCGCATGCCCACGCTGAGCGGTGTACGCCGCCGCGGCATAACTCCCGAAGCGCTGCGTGAATTCTGCGCGCGC
ATCGGCGTGGCCAAAGCTGACAGCATGGTGGACTACGGTCTGCTGGAATTCTGCGCACGCGAACACCTGAACCGCATAGC
CCCCCGCCTGATGGCGGTGCTGGATCCTGTAAAGGTGGTTATCGAAAACTATCCGGAAGGACAGGTGGAAGAATTTGAAA
TGCCCCTGAATCCCGAAGACGAATCACAGGGTACCCGCATGGTTCCGTTCTCGCGTGAACTGTACATCGAAAGGGATGAC
TTCCGCGAACAGGCACCCAACAAAAAATTCTTCCGCCTTGCCATCGGCAAGGAAGTGCGGCTGCGCTATGCCTATTACAT
CACCTGCAACGATGTGGTGAAGGACGAACACGGCAACATAACCGAACTGCGCTGCACGTACGACCCCGCCACCCGCGGCG
GCTGGTCCGACGACGGACGCAAGGTCAAAGGGACCCTGCACTGGGTTTCCGCCGCCCACGCCGTACCGGCTCAGGTACGC
CTGTACGACAAGCTGTTCACCGTGGAAAATCCCAATGCCGCCGAAGAAGGCAAAACATTTGTGGATTATCTGAATCCCGA
TTCACTTTCCGTGGTGACGGGCTACCTTGAACCGGCACTGAAAAACATGGAACACGGCAGCCGGGTGCAGTTTGAACGCA
TCGGCTATTTCTGCGTCGACCCCGATTCCACAGCGGACGCTCCCGTGTTCAACCGTACCGTGACGCTGAAGGACAGCTGG
GCCAAGATAGCCAACAAGCAGGACTGA

Upstream 100 bases:

>100_bases
TTTGCCGTATCTCCAGACGCATCATTTTGCTTTTCATTGCCAAAGACGCGCGGCACCGTGTTGCCCGGACCTGCGCATAT
ATACACGAGGAATACCATCC

Downstream 100 bases:

>100_bases
TCCGCCGGCACCGTCTCCGCCTGACAAAGCGCCGCACCTTTCGGGTGCGGCGCTTTTTTATGCCCGGCAGCACGCAAAAA
AACACCGCACACGGCCCTGC

Product: glutaminyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamine--tRNA ligase; GlnRS [H]

Number of amino acids: Translated: 568; Mature: 567

Protein sequence:

>568_residues
MSINPADTDKKKEAGVDFIRAIVNEDMKTGKYQGRVHTRFPPEPNGYLHLGHAKSICLNFGLAQEYGGKCNLRLDDTNPL
KETPEYVESIKTDVRWLGFDWEDRMFHASDYFERLFELAVQLIKAGKAYVDDLTPEQIREYRGTLKEPGKNSPYRDRSAE
ENLDLFMRMRNGEFEDGSRVLRAKIDMASPNMVMRDPTIYRIRRAHHHRTGDAWCIYPMYDFTHCISDSIEGITHSICTL
EFENNRELYDWMLNTLELYHPQQIEFARLNLTHTVLSKRKLIQLVEEGHVNGWDDPRMPTLSGVRRRGITPEALREFCAR
IGVAKADSMVDYGLLEFCAREHLNRIAPRLMAVLDPVKVVIENYPEGQVEEFEMPLNPEDESQGTRMVPFSRELYIERDD
FREQAPNKKFFRLAIGKEVRLRYAYYITCNDVVKDEHGNITELRCTYDPATRGGWSDDGRKVKGTLHWVSAAHAVPAQVR
LYDKLFTVENPNAAEEGKTFVDYLNPDSLSVVTGYLEPALKNMEHGSRVQFERIGYFCVDPDSTADAPVFNRTVTLKDSW
AKIANKQD

Sequences:

>Translated_568_residues
MSINPADTDKKKEAGVDFIRAIVNEDMKTGKYQGRVHTRFPPEPNGYLHLGHAKSICLNFGLAQEYGGKCNLRLDDTNPL
KETPEYVESIKTDVRWLGFDWEDRMFHASDYFERLFELAVQLIKAGKAYVDDLTPEQIREYRGTLKEPGKNSPYRDRSAE
ENLDLFMRMRNGEFEDGSRVLRAKIDMASPNMVMRDPTIYRIRRAHHHRTGDAWCIYPMYDFTHCISDSIEGITHSICTL
EFENNRELYDWMLNTLELYHPQQIEFARLNLTHTVLSKRKLIQLVEEGHVNGWDDPRMPTLSGVRRRGITPEALREFCAR
IGVAKADSMVDYGLLEFCAREHLNRIAPRLMAVLDPVKVVIENYPEGQVEEFEMPLNPEDESQGTRMVPFSRELYIERDD
FREQAPNKKFFRLAIGKEVRLRYAYYITCNDVVKDEHGNITELRCTYDPATRGGWSDDGRKVKGTLHWVSAAHAVPAQVR
LYDKLFTVENPNAAEEGKTFVDYLNPDSLSVVTGYLEPALKNMEHGSRVQFERIGYFCVDPDSTADAPVFNRTVTLKDSW
AKIANKQD
>Mature_567_residues
SINPADTDKKKEAGVDFIRAIVNEDMKTGKYQGRVHTRFPPEPNGYLHLGHAKSICLNFGLAQEYGGKCNLRLDDTNPLK
ETPEYVESIKTDVRWLGFDWEDRMFHASDYFERLFELAVQLIKAGKAYVDDLTPEQIREYRGTLKEPGKNSPYRDRSAEE
NLDLFMRMRNGEFEDGSRVLRAKIDMASPNMVMRDPTIYRIRRAHHHRTGDAWCIYPMYDFTHCISDSIEGITHSICTLE
FENNRELYDWMLNTLELYHPQQIEFARLNLTHTVLSKRKLIQLVEEGHVNGWDDPRMPTLSGVRRRGITPEALREFCARI
GVAKADSMVDYGLLEFCAREHLNRIAPRLMAVLDPVKVVIENYPEGQVEEFEMPLNPEDESQGTRMVPFSRELYIERDDF
REQAPNKKFFRLAIGKEVRLRYAYYITCNDVVKDEHGNITELRCTYDPATRGGWSDDGRKVKGTLHWVSAAHAVPAQVRL
YDKLFTVENPNAAEEGKTFVDYLNPDSLSVVTGYLEPALKNMEHGSRVQFERIGYFCVDPDSTADAPVFNRTVTLKDSWA
KIANKQD

Specific function: Unknown

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI4826960, Length=533, Percent_Identity=46.5290806754221, Blast_Score=447, Evalue=1e-125,
Organism=Homo sapiens, GI62241042, Length=526, Percent_Identity=34.7908745247148, Blast_Score=289, Evalue=4e-78,
Organism=Escherichia coli, GI1786895, Length=551, Percent_Identity=62.0689655172414, Blast_Score=712, Evalue=0.0,
Organism=Escherichia coli, GI1788743, Length=313, Percent_Identity=25.8785942492013, Blast_Score=83, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17543332, Length=539, Percent_Identity=43.2282003710575, Blast_Score=432, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17511007, Length=520, Percent_Identity=35.1923076923077, Blast_Score=290, Evalue=2e-78,
Organism=Saccharomyces cerevisiae, GI6324742, Length=567, Percent_Identity=42.5044091710758, Blast_Score=429, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6321192, Length=512, Percent_Identity=38.0859375, Blast_Score=327, Evalue=3e-90,
Organism=Drosophila melanogaster, GI17864488, Length=532, Percent_Identity=43.421052631579, Blast_Score=437, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24649466, Length=545, Percent_Identity=35.9633027522936, Blast_Score=297, Evalue=1e-80,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR004514
- InterPro:   IPR022861
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR020056
- InterPro:   IPR011035
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C [H]

EC number: =6.1.1.18 [H]

Molecular weight: Translated: 65575; Mature: 65444

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00435 PEROXIDASE_1 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSINPADTDKKKEAGVDFIRAIVNEDMKTGKYQGRVHTRFPPEPNGYLHLGHAKSICLNF
CCCCCCCCCCHHHHHHHHHHHHHHCHHHCCCCCCEEECCCCCCCCCEEEECCHHHHHHHC
GLAQEYGGKCNLRLDDTNPLKETPEYVESIKTDVRWLGFDWEDRMFHASDYFERLFELAV
CCHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCEEECCCHHHCEEHHHHHHHHHHHHHH
QLIKAGKAYVDDLTPEQIREYRGTLKEPGKNSPYRDRSAEENLDLFMRMRNGEFEDGSRV
HHHHCCCHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHEEECCCCCCCCCCE
LRAKIDMASPNMVMRDPTIYRIRRAHHHRTGDAWCIYPMYDFTHCISDSIEGITHSICTL
EEEEECCCCCCEEEECCHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHEEEEEEE
EFENNRELYDWMLNTLELYHPQQIEFARLNLTHTVLSKRKLIQLVEEGHVNGWDDPRMPT
EECCCCHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
LSGVRRRGITPEALREFCARIGVAKADSMVDYGLLEFCAREHLNRIAPRLMAVLDPVKVV
HHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IENYPEGQVEEFEMPLNPEDESQGTRMVPFSRELYIERDDFREQAPNKKFFRLAIGKEVR
HHCCCCCCHHHCCCCCCCCCCCCCCEECCCCHHEEEECHHHHHHCCCCEEEEEECCCEEE
LRYAYYITCNDVVKDEHGNITELRCTYDPATRGGWSDDGRKVKGTLHWVSAAHAVPAQVR
EEEEEEEEECHHHCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEEEEEHHHHHCCCHHHE
LYDKLFTVENPNAAEEGKTFVDYLNPDSLSVVTGYLEPALKNMEHGSRVQFERIGYFCVD
EEHHHEECCCCCCHHCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEHHHCCEEEEC
PDSTADAPVFNRTVTLKDSWAKIANKQD
CCCCCCCCCCCEEEEECHHHHHHCCCCC
>Mature Secondary Structure 
SINPADTDKKKEAGVDFIRAIVNEDMKTGKYQGRVHTRFPPEPNGYLHLGHAKSICLNF
CCCCCCCCCHHHHHHHHHHHHHHCHHHCCCCCCEEECCCCCCCCCEEEECCHHHHHHHC
GLAQEYGGKCNLRLDDTNPLKETPEYVESIKTDVRWLGFDWEDRMFHASDYFERLFELAV
CCHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCEEECCCHHHCEEHHHHHHHHHHHHHH
QLIKAGKAYVDDLTPEQIREYRGTLKEPGKNSPYRDRSAEENLDLFMRMRNGEFEDGSRV
HHHHCCCHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHEEECCCCCCCCCCE
LRAKIDMASPNMVMRDPTIYRIRRAHHHRTGDAWCIYPMYDFTHCISDSIEGITHSICTL
EEEEECCCCCCEEEECCHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHEEEEEEE
EFENNRELYDWMLNTLELYHPQQIEFARLNLTHTVLSKRKLIQLVEEGHVNGWDDPRMPT
EECCCCHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
LSGVRRRGITPEALREFCARIGVAKADSMVDYGLLEFCAREHLNRIAPRLMAVLDPVKVV
HHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IENYPEGQVEEFEMPLNPEDESQGTRMVPFSRELYIERDDFREQAPNKKFFRLAIGKEVR
HHCCCCCCHHHCCCCCCCCCCCCCCEECCCCHHEEEECHHHHHHCCCCEEEEEECCCEEE
LRYAYYITCNDVVKDEHGNITELRCTYDPATRGGWSDDGRKVKGTLHWVSAAHAVPAQVR
EEEEEEEEECHHHCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEEEEEHHHHHCCCHHHE
LYDKLFTVENPNAAEEGKTFVDYLNPDSLSVVTGYLEPALKNMEHGSRVQFERIGYFCVD
EEHHHEECCCCCCHHCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEHHHCCEEEEC
PDSTADAPVFNRTVTLKDSWAKIANKQD
CCCCCCCCCCCEEEEECHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12835416 [H]