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The map label for this gene is vipB [H]

Identifier: 78355405

GI number: 78355405

Start: 346760

End: 347785

Strand: Reverse

Name: vipB [H]

Synonym: Dde_0358

Alternate gene names: 78355405

Gene position: 347785-346760 (Counterclockwise)

Preceding gene: 78355407

Following gene: 78355403

Centisome position: 9.32

GC content: 59.06

Gene sequence:

>1026_bases
ATGTCCAGATATACCGAACTGCTCCGTCAATTGCAGGAGGCTCCCCGCACATGGCTCATAACCGGCGTGGCGGGCTTCAT
AGGCTCCAACCTGCTTGAAACCCTGCTCAGCCACGGCCAGACCGTCACCGGTCTTGATAATTTCATGACCGGACACAGAA
AAAACCTCGACATGGTGCGCGACGCTGTCACTCCGGAACAGTGGAGCCGCTTCCGGTTCATCGAAGGTGACATCCGCTCG
CTGGAAACCTGCCGCGAAGCGTGCGCCGGCGCGGACTACGTTCTGCATCAGGCTGCGCTCGGTTCGGTACCGCGTTCCAT
TGATGACCCGCTGCTGACCAATACCTGTAACATCGACGGCTTTGTAAACATGCTGGTGGCCGCACGCGATGCGGATGTCC
GCAGATTTGTCTACGCCGCTTCCAGCTCGACCTACGGTGACGAGCCCAACCTGCCCAAGCAGGAAGACCGCATCGGCAGG
CCGCTTTCGCCGTATGCCGTTACAAAGTATGTCAACGAACTGTATGCAGACGTCTTTGCCAGAACCTACGGCATGGAATG
CACCGGACTGCGCTATTTCAACGTATTCGGCAAACGTCAGGATCCTGAAGGCGCCTATGCCGCGGTCATCCCGCTGTGGT
TTGCCGGTCTGCTGCGCGACCAGCCAGTATTCATAAACGGTGACGGCGAAACCAGCCGCGACTTCTGTCACATAGACAAC
ACTGTGCAGGCCAACCTGCTGGCGGCCACCGCTCCCGATCCCGAAGCAACCAATACTGTATACAACGTGGCCTTCGGAGA
ACGCACATCGCTGACCGAACTGTACGGCCTCATCCGCGAGGAAGTGGCGCGCCACAATCCGCATGCGTCAGAAAAAGAAG
CACAGTACCGCGATTTCCGCGCGGGCGACGTGCGCCACTCTCTGGCTGATATCAGCCGTGCCCGCACCCTGCTGGGCTAT
GATCCGCAGATTTCCGTCCGTCAGGGGCTGCGCATTTCCGGCGACTGGTACGCAGCAAACCTGTAG

Upstream 100 bases:

>100_bases
CGGAAGGAGGCAGCCGGAAGATACAGCAGAGTCCGCAGCATACGCCCTGCGGCAGGTGTCCGCAACTCCGGCACATCACA
CGTCCGACAGGAGACAACGC

Downstream 100 bases:

>100_bases
CAGCCTGCCTGCATAACAAGAACAAAGCCACGCACGGTGCCCATAGGCCCGTGCGTGGCTTTGTTCTTATTTTCCTCTTC
CCGCAAAAAGGTGATTTTCC

Product: NAD-dependent epimerase/dehydratase family protein

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MSRYTELLRQLQEAPRTWLITGVAGFIGSNLLETLLSHGQTVTGLDNFMTGHRKNLDMVRDAVTPEQWSRFRFIEGDIRS
LETCREACAGADYVLHQAALGSVPRSIDDPLLTNTCNIDGFVNMLVAARDADVRRFVYAASSSTYGDEPNLPKQEDRIGR
PLSPYAVTKYVNELYADVFARTYGMECTGLRYFNVFGKRQDPEGAYAAVIPLWFAGLLRDQPVFINGDGETSRDFCHIDN
TVQANLLAATAPDPEATNTVYNVAFGERTSLTELYGLIREEVARHNPHASEKEAQYRDFRAGDVRHSLADISRARTLLGY
DPQISVRQGLRISGDWYAANL

Sequences:

>Translated_341_residues
MSRYTELLRQLQEAPRTWLITGVAGFIGSNLLETLLSHGQTVTGLDNFMTGHRKNLDMVRDAVTPEQWSRFRFIEGDIRS
LETCREACAGADYVLHQAALGSVPRSIDDPLLTNTCNIDGFVNMLVAARDADVRRFVYAASSSTYGDEPNLPKQEDRIGR
PLSPYAVTKYVNELYADVFARTYGMECTGLRYFNVFGKRQDPEGAYAAVIPLWFAGLLRDQPVFINGDGETSRDFCHIDN
TVQANLLAATAPDPEATNTVYNVAFGERTSLTELYGLIREEVARHNPHASEKEAQYRDFRAGDVRHSLADISRARTLLGY
DPQISVRQGLRISGDWYAANL
>Mature_340_residues
SRYTELLRQLQEAPRTWLITGVAGFIGSNLLETLLSHGQTVTGLDNFMTGHRKNLDMVRDAVTPEQWSRFRFIEGDIRSL
ETCREACAGADYVLHQAALGSVPRSIDDPLLTNTCNIDGFVNMLVAARDADVRRFVYAASSSTYGDEPNLPKQEDRIGRP
LSPYAVTKYVNELYADVFARTYGMECTGLRYFNVFGKRQDPEGAYAAVIPLWFAGLLRDQPVFINGDGETSRDFCHIDNT
VQANLLAATAPDPEATNTVYNVAFGERTSLTELYGLIREEVARHNPHASEKEAQYRDFRAGDVRHSLADISRARTLLGYD
PQISVRQGLRISGDWYAANL

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=333, Percent_Identity=27.3273273273273, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI7657641, Length=330, Percent_Identity=25.4545454545455, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI56237023, Length=329, Percent_Identity=25.2279635258359, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI56118217, Length=329, Percent_Identity=25.2279635258359, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI189083684, Length=329, Percent_Identity=25.2279635258359, Blast_Score=78, Evalue=9e-15,
Organism=Escherichia coli, GI1786974, Length=320, Percent_Identity=27.1875, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI48994969, Length=356, Percent_Identity=29.2134831460674, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788353, Length=349, Percent_Identity=27.5071633237822, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17539532, Length=340, Percent_Identity=25.5882352941176, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71982035, Length=347, Percent_Identity=24.7838616714697, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71982038, Length=347, Percent_Identity=24.7838616714697, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17568069, Length=344, Percent_Identity=24.4186046511628, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI115532424, Length=328, Percent_Identity=23.780487804878, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17539422, Length=333, Percent_Identity=25.8258258258258, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI133901788, Length=333, Percent_Identity=25.8258258258258, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI133901786, Length=333, Percent_Identity=25.8258258258258, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI133901790, Length=333, Percent_Identity=25.8258258258258, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17507723, Length=328, Percent_Identity=26.5243902439024, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17539424, Length=333, Percent_Identity=25.8258258258258, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=358, Percent_Identity=24.8603351955307, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356223, Length=366, Percent_Identity=25.4098360655738, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19923002, Length=356, Percent_Identity=28.0898876404494, Blast_Score=96, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 38129; Mature: 37998

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRYTELLRQLQEAPRTWLITGVAGFIGSNLLETLLSHGQTVTGLDNFMTGHRKNLDMVR
CCHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCCCEEECHHHHHCCCCCCHHHHH
DAVTPEQWSRFRFIEGDIRSLETCREACAGADYVLHQAALGSVPRSIDDPLLTNTCNIDG
HHCCHHHHHHEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHCCCCCHHH
FVNMLVAARDADVRRFVYAASSSTYGDEPNLPKQEDRIGRPLSPYAVTKYVNELYADVFA
HHHHHHHHCCCHHHHHEEECCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHH
RTYGMECTGLRYFNVFGKRQDPEGAYAAVIPLWFAGLLRDQPVFINGDGETSRDFCHIDN
HHHCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEECCC
TVQANLLAATAPDPEATNTVYNVAFGERTSLTELYGLIREEVARHNPHASEKEAQYRDFR
CCEEEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCC
AGDVRHSLADISRARTLLGYDPQISVRQGLRISGDWYAANL
CHHHHHHHHHHHHHHHHHCCCCCCHHHCCCEECCCEEECCC
>Mature Secondary Structure 
SRYTELLRQLQEAPRTWLITGVAGFIGSNLLETLLSHGQTVTGLDNFMTGHRKNLDMVR
CHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCCCEEECHHHHHCCCCCCHHHHH
DAVTPEQWSRFRFIEGDIRSLETCREACAGADYVLHQAALGSVPRSIDDPLLTNTCNIDG
HHCCHHHHHHEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHCCCCCHHH
FVNMLVAARDADVRRFVYAASSSTYGDEPNLPKQEDRIGRPLSPYAVTKYVNELYADVFA
HHHHHHHHCCCHHHHHEEECCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHH
RTYGMECTGLRYFNVFGKRQDPEGAYAAVIPLWFAGLLRDQPVFINGDGETSRDFCHIDN
HHHCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEECCC
TVQANLLAATAPDPEATNTVYNVAFGERTSLTELYGLIREEVARHNPHASEKEAQYRDFR
CCEEEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCC
AGDVRHSLADISRARTLLGYDPQISVRQGLRISGDWYAANL
CHHHHHHHHHHHHHHHHHCCCCCCHHHCCCEECCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8248629; 8331073; 11677608; 12644504 [H]