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The map label for this gene is hisF

Identifier: 78355378

GI number: 78355378

Start: 318056

End: 318835

Strand: Reverse

Name: hisF

Synonym: Dde_0331

Alternate gene names: 78355378

Gene position: 318835-318056 (Counterclockwise)

Preceding gene: 78355379

Following gene: 78355377

Centisome position: 8.55

GC content: 57.44

Gene sequence:

>780_bases
ATGCTCAGTAAACGGATTATCCCCTGCCTTGACGTGCGTGACGGCCGCCTGACCAAGGGCATAAAATTTCAGGGCAATGT
GGACATAGGCGACCCCGTGGAAAGCGCCAGACGGTACTACGAACAGGGCGCCGACGAGATTGTTTTCTACGATATCACCG
CATCGCACGAAGGGCGCGGTATCTTTCTTGATATTGTGGAAAAGGTGGCTTCGAGCATTTTCATACCTTTTTCCGTGGGC
GGCGGCATAAACACCGTGGACGATATGCGCGACGCGCTCAACGCCGGTGCGGAAAAGGTTTCGGTCAATTCCGGTGCGGT
GAAAGACCCTGACATCATCAGCAAAGGGGCCGCCCGTTTCGGTTCGCAGGCCATTGTGCTGGGCATGGATGTCAAACGCG
TGGCCCCGACAGAAGCCATCCCCTCGGGGTACGAAATCGTCATCCACGGCGGACGCAAACACATGGGCATGGACGCGCTG
GACTGGGCGAAAACAGCAGAAGTGCTGGGTGCCGGTGAAATCTGCGTCAACTCCATAGACGCCGACGGTACCAAGGACGG
CTACGAACTGACCCTGACCCGCATGATAAGCGATGCCGTGCAGATTCCCGTCATCGCCTCGGGCGGGGCCGGACATCCTG
CGCATATGTATGACGCCCTGACCAAAGGCGGCGCTTCTGCAGCGCTCATCGCGTCCATTGTGCATTACGGCGAATACACC
ATACCCGACCTGAAAAAACAGATTCAGGCCATGGGCTGCAAAATGCGGCTGACCTGGTAA

Upstream 100 bases:

>100_bases
GGGCCGGGGCTGTGGGCCGTCCAGTTCCATCCGGAAAAAAGCGGCAGACCCGGACTGAAGCTGCTCAGCAACTTCCACGC
ATACTGCAAGGAGGCCGCCA

Downstream 100 bases:

>100_bases
CACCCCTGCAATGCAGGCCGCCGGCAATGCACACCCGGTGAAAAGCGGGTTGCGTACCGGCCCCGGACTGCCGTCGGCAA
TGCGCTTTAACGCACGCCAC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MLSKRIIPCLDVRDGRLTKGIKFQGNVDIGDPVESARRYYEQGADEIVFYDITASHEGRGIFLDIVEKVASSIFIPFSVG
GGINTVDDMRDALNAGAEKVSVNSGAVKDPDIISKGAARFGSQAIVLGMDVKRVAPTEAIPSGYEIVIHGGRKHMGMDAL
DWAKTAEVLGAGEICVNSIDADGTKDGYELTLTRMISDAVQIPVIASGGAGHPAHMYDALTKGGASAALIASIVHYGEYT
IPDLKKQIQAMGCKMRLTW

Sequences:

>Translated_259_residues
MLSKRIIPCLDVRDGRLTKGIKFQGNVDIGDPVESARRYYEQGADEIVFYDITASHEGRGIFLDIVEKVASSIFIPFSVG
GGINTVDDMRDALNAGAEKVSVNSGAVKDPDIISKGAARFGSQAIVLGMDVKRVAPTEAIPSGYEIVIHGGRKHMGMDAL
DWAKTAEVLGAGEICVNSIDADGTKDGYELTLTRMISDAVQIPVIASGGAGHPAHMYDALTKGGASAALIASIVHYGEYT
IPDLKKQIQAMGCKMRLTW
>Mature_259_residues
MLSKRIIPCLDVRDGRLTKGIKFQGNVDIGDPVESARRYYEQGADEIVFYDITASHEGRGIFLDIVEKVASSIFIPFSVG
GGINTVDDMRDALNAGAEKVSVNSGAVKDPDIISKGAARFGSQAIVLGMDVKRVAPTEAIPSGYEIVIHGGRKHMGMDAL
DWAKTAEVLGAGEICVNSIDADGTKDGYELTLTRMISDAVQIPVIASGGAGHPAHMYDALTKGGASAALIASIVHYGEYT
IPDLKKQIQAMGCKMRLTW

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=261, Percent_Identity=41.7624521072797, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI87082028, Length=243, Percent_Identity=26.7489711934156, Blast_Score=77, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=34.0764331210191, Blast_Score=164, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_DESDG (Q316L4)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_386827.1
- ProteinModelPortal:   Q316L4
- SMR:   Q316L4
- STRING:   Q316L4
- GeneID:   3756380
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_0331
- NMPDR:   fig|207559.3.peg.1841
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- ProtClustDB:   PRK02083
- BioCyc:   DDES207559:DDE_0331-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27585; Mature: 27585

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSKRIIPCLDVRDGRLTKGIKFQGNVDIGDPVESARRYYEQGADEIVFYDITASHEGRG
CCCCCCCCEECCCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCE
IFLDIVEKVASSIFIPFSVGGGINTVDDMRDALNAGAEKVSVNSGAVKDPDIISKGAARF
EHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHC
GSQAIVLGMDVKRVAPTEAIPSGYEIVIHGGRKHMGMDALDWAKTAEVLGAGEICVNSID
CCCEEEEECCHHHCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCHHEEECCC
ADGTKDGYELTLTRMISDAVQIPVIASGGAGHPAHMYDALTKGGASAALIASIVHYGEYT
CCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCC
IPDLKKQIQAMGCKMRLTW
CHHHHHHHHHCCCEEEECC
>Mature Secondary Structure
MLSKRIIPCLDVRDGRLTKGIKFQGNVDIGDPVESARRYYEQGADEIVFYDITASHEGRG
CCCCCCCCEECCCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCE
IFLDIVEKVASSIFIPFSVGGGINTVDDMRDALNAGAEKVSVNSGAVKDPDIISKGAARF
EHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHC
GSQAIVLGMDVKRVAPTEAIPSGYEIVIHGGRKHMGMDALDWAKTAEVLGAGEICVNSID
CCCEEEEECCHHHCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCHHEEECCC
ADGTKDGYELTLTRMISDAVQIPVIASGGAGHPAHMYDALTKGGASAALIASIVHYGEYT
CCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCC
IPDLKKQIQAMGCKMRLTW
CHHHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA