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The map label for this gene is hisH

Identifier: 78355379

GI number: 78355379

Start: 318825

End: 319466

Strand: Reverse

Name: hisH

Synonym: Dde_0332

Alternate gene names: 78355379

Gene position: 319466-318825 (Counterclockwise)

Preceding gene: 78355385

Following gene: 78355378

Centisome position: 8.56

GC content: 59.03

Gene sequence:

>642_bases
ATGCTTGCGATCCTGAAATACAAGGCCGGCAACCAGACAAGCGTGCGCCGTGCCCTGAACCACATCGGGATCCCCAACGT
GATCACGGCGGACCCCGCCGAGATCGCCGCCTCGGAAGGCATCATCTTTCCCGGCGTGGGGGCCGCCGGACAGGCCATGG
ACGAACTGACGGCCGCCGGACTTGACGCTGTGCTCAAAGAGCAGGTGGAAGCAGGCAAACCGCTGCTGGGCATCTGTGTA
GGCTGCCAGATAATGCTGGACTACAGTCAGGAAAACGATACCCGTACGCTGGGCATCATTCCCGGAGAATGCCATCTGTT
CAATCCCGCGCTGGAAGACGAAACCGGCGCGCCCATCCGCGTACCGCACATGGGCTGGAACAGAGTGATACCCAGAAAGC
CGTGCGCACTTTTCAAAGGCATTTCCGATGATGCCGAATTTTATTTTGTGCACAGCTACTTTCCCAACCCGCAGCCGGAC
TATGTCATTGCCACCACGACGTACGGCAAGGAATTCTGTTCTGTTCACGGCGGGCCGGGGCTGTGGGCCGTCCAGTTCCA
TCCGGAAAAAAGCGGCAGACCCGGACTGAAGCTGCTCAGCAACTTCCACGCATACTGCAAGGAGGCCGCCAATGCTCAGT
AA

Upstream 100 bases:

>100_bases
AGAATGTGCCCGCGACGTTATAGAGTGCAGGAAACGCTTGCTCTTTACGGCGTGAAGCTGATAATTGCCGAGTACCTAAA
CCCCGCAAGAGGAGGGAACC

Downstream 100 bases:

>100_bases
ACGGATTATCCCCTGCCTTGACGTGCGTGACGGCCGCCTGACCAAGGGCATAAAATTTCAGGGCAATGTGGACATAGGCG
ACCCCGTGGAAAGCGCCAGA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MLAILKYKAGNQTSVRRALNHIGIPNVITADPAEIAASEGIIFPGVGAAGQAMDELTAAGLDAVLKEQVEAGKPLLGICV
GCQIMLDYSQENDTRTLGIIPGECHLFNPALEDETGAPIRVPHMGWNRVIPRKPCALFKGISDDAEFYFVHSYFPNPQPD
YVIATTTYGKEFCSVHGGPGLWAVQFHPEKSGRPGLKLLSNFHAYCKEAANAQ

Sequences:

>Translated_213_residues
MLAILKYKAGNQTSVRRALNHIGIPNVITADPAEIAASEGIIFPGVGAAGQAMDELTAAGLDAVLKEQVEAGKPLLGICV
GCQIMLDYSQENDTRTLGIIPGECHLFNPALEDETGAPIRVPHMGWNRVIPRKPCALFKGISDDAEFYFVHSYFPNPQPD
YVIATTTYGKEFCSVHGGPGLWAVQFHPEKSGRPGLKLLSNFHAYCKEAANAQ
>Mature_213_residues
MLAILKYKAGNQTSVRRALNHIGIPNVITADPAEIAASEGIIFPGVGAAGQAMDELTAAGLDAVLKEQVEAGKPLLGICV
GCQIMLDYSQENDTRTLGIIPGECHLFNPALEDETGAPIRVPHMGWNRVIPRKPCALFKGISDDAEFYFVHSYFPNPQPD
YVIATTTYGKEFCSVHGGPGLWAVQFHPEKSGRPGLKLLSNFHAYCKEAANAQ

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=202, Percent_Identity=39.1089108910891, Blast_Score=124, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6319725, Length=215, Percent_Identity=33.4883720930233, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_DESDG (Q316L3)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_386828.1
- ProteinModelPortal:   Q316L3
- SMR:   Q316L3
- STRING:   Q316L3
- GeneID:   3756381
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_0332
- NMPDR:   fig|207559.3.peg.1842
- eggNOG:   COG0118
- HOGENOM:   HBG292341
- OMA:   SVRFAFE
- ProtClustDB:   PRK13141
- BioCyc:   DDES207559:DDE_0332-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 22978; Mature: 22978

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 79-79 ACT_SITE 187-187 ACT_SITE 189-189

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAILKYKAGNQTSVRRALNHIGIPNVITADPAEIAASEGIIFPGVGAAGQAMDELTAAG
CEEEEEECCCCHHHHHHHHHHCCCCCCEECCHHHHHHCCCEEECCCCCCHHHHHHHHHHH
LDAVLKEQVEAGKPLLGICVGCQIMLDYSQENDTRTLGIIPGECHLFNPALEDETGAPIR
HHHHHHHHHHHCCCEEEEEECEEEEEEECCCCCCEEEEEECCCCEEECCCCCCCCCCCEE
VPHMGWNRVIPRKPCALFKGISDDAEFYFVHSYFPNPQPDYVIATTTYGKEFCSVHGGPG
ECCCCCCCCCCCCCHHHHCCCCCCCEEEEEEEECCCCCCCEEEEEECCCHHHHHCCCCCC
LWAVQFHPEKSGRPGLKLLSNFHAYCKEAANAQ
EEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLAILKYKAGNQTSVRRALNHIGIPNVITADPAEIAASEGIIFPGVGAAGQAMDELTAAG
CEEEEEECCCCHHHHHHHHHHCCCCCCEECCHHHHHHCCCEEECCCCCCHHHHHHHHHHH
LDAVLKEQVEAGKPLLGICVGCQIMLDYSQENDTRTLGIIPGECHLFNPALEDETGAPIR
HHHHHHHHHHHCCCEEEEEECEEEEEEECCCCCCEEEEEECCCCEEECCCCCCCCCCCEE
VPHMGWNRVIPRKPCALFKGISDDAEFYFVHSYFPNPQPDYVIATTTYGKEFCSVHGGPG
ECCCCCCCCCCCCCHHHHCCCCCCCEEEEEEEECCCCCCCEEEEEECCCHHHHHCCCCCC
LWAVQFHPEKSGRPGLKLLSNFHAYCKEAANAQ
EEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA