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The map label for this gene is grpE

Identifier: 78224716

GI number: 78224716

Start: 3967385

End: 3967954

Strand: Reverse

Name: grpE

Synonym: Gmet_3533

Alternate gene names: 78224716

Gene position: 3967954-3967385 (Counterclockwise)

Preceding gene: 78224717

Following gene: 78224715

Centisome position: 99.26

GC content: 61.58

Gene sequence:

>570_bases
GTGGACAAAAAAAAGCACGGCTCCCACGCAGGAGCGCACCATACCGATGAGCCCGCAGCCGAAACCGTGGCCCCGGCAGC
CGAAGGGGCACCTGCCGCCGCCGACCGAGTGAAGGAGCTGGAGGAGGCCCTGGCCGCCAAGGAGGCCGAAGCGGCCGCCA
ACTGGGATAAGTTTGTCCGGGAGCGGGCCGACCTGGAGAACTACCGCAAGCGGACCCAGAAGGAGAAGGAAGAGCTCCTC
AAGTACGGCAACGAAAGCCTTATCGTAGAGATCCTCCCGGTGGTGGACAACATGGAGCGAGCCCTGGATCATTCCGATGA
TGATAGTGCCTCGGCGGTCATCGAGGGGGTCCGGATGACCCTAAACATGCTCCTGTCCACCCTGAAGAAGTTTGGGGTCA
CGGTTGTGGAAGCCGAAAAGGGAACCCCCTTCGATCCGGCGGTTCACCAGGCCATGTGTCAGGTGGAAAACACCGATGTG
CCGGCCAACAGCGTGGTGGAGATCTTCCAGAAGGGGTACCTCCTGAACGAGCGGCTGATCCGCCCTGCCATGGTTTCGGT
GTCCAAGTAG

Upstream 100 bases:

>100_bases
CCCGCATGGGGTACGGCAAGGTGATCCCCATCGTCGACTACACCGCGAAGCTTGTGAGCAAGCTGCTCGAAGACGAGTAG
AGTAAGAAAGGAAAACCATC

Downstream 100 bases:

>100_bases
ATTTTTTTCGCCGTCCCCTTGTTTTTCACCGGGGCGATAACTAGGTTCTGTCTCAGAGACACACTAAAGGAGGAACAGTA
ACATGAGCAAAGTCATCGGT

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 189; Mature: 189

Protein sequence:

>189_residues
MDKKKHGSHAGAHHTDEPAAETVAPAAEGAPAAADRVKELEEALAAKEAEAAANWDKFVRERADLENYRKRTQKEKEELL
KYGNESLIVEILPVVDNMERALDHSDDDSASAVIEGVRMTLNMLLSTLKKFGVTVVEAEKGTPFDPAVHQAMCQVENTDV
PANSVVEIFQKGYLLNERLIRPAMVSVSK

Sequences:

>Translated_189_residues
MDKKKHGSHAGAHHTDEPAAETVAPAAEGAPAAADRVKELEEALAAKEAEAAANWDKFVRERADLENYRKRTQKEKEELL
KYGNESLIVEILPVVDNMERALDHSDDDSASAVIEGVRMTLNMLLSTLKKFGVTVVEAEKGTPFDPAVHQAMCQVENTDV
PANSVVEIFQKGYLLNERLIRPAMVSVSK
>Mature_189_residues
MDKKKHGSHAGAHHTDEPAAETVAPAAEGAPAAADRVKELEEALAAKEAEAAANWDKFVRERADLENYRKRTQKEKEELL
KYGNESLIVEILPVVDNMERALDHSDDDSASAVIEGVRMTLNMLLSTLKKFGVTVVEAEKGTPFDPAVHQAMCQVENTDV
PANSVVEIFQKGYLLNERLIRPAMVSVSK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Escherichia coli, GI1788967, Length=164, Percent_Identity=42.6829268292683, Blast_Score=123, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6324806, Length=182, Percent_Identity=30.7692307692308, Blast_Score=86, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24653432, Length=179, Percent_Identity=34.6368715083799, Blast_Score=88, Evalue=4e-18,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_GEOMG (Q39PT6)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_386463.1
- ProteinModelPortal:   Q39PT6
- SMR:   Q39PT6
- STRING:   Q39PT6
- GeneID:   3739792
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_3533
- NMPDR:   fig|269799.3.peg.3501
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   HEAIAYV
- PhylomeDB:   Q39PT6
- ProtClustDB:   PRK14158
- BioCyc:   GMET269799:GMET_3533-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 20764; Mature: 20764

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKKKHGSHAGAHHTDEPAAETVAPAAEGAPAAADRVKELEEALAAKEAEAAANWDKFVR
CCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERADLENYRKRTQKEKEELLKYGNESLIVEILPVVDNMERALDHSDDDSASAVIEGVRMT
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LNMLLSTLKKFGVTVVEAEKGTPFDPAVHQAMCQVENTDVPANSVVEIFQKGYLLNERLI
HHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCHHHHHHHH
RPAMVSVSK
HHHHHHCCC
>Mature Secondary Structure
MDKKKHGSHAGAHHTDEPAAETVAPAAEGAPAAADRVKELEEALAAKEAEAAANWDKFVR
CCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERADLENYRKRTQKEKEELLKYGNESLIVEILPVVDNMERALDHSDDDSASAVIEGVRMT
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LNMLLSTLKKFGVTVVEAEKGTPFDPAVHQAMCQVENTDVPANSVVEIFQKGYLLNERLI
HHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCHHHHHHHH
RPAMVSVSK
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA