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The map label for this gene is cofH [H]

Identifier: 78224581

GI number: 78224581

Start: 3805049

End: 3806131

Strand: Reverse

Name: cofH [H]

Synonym: Gmet_3391

Alternate gene names: 78224581

Gene position: 3806131-3805049 (Counterclockwise)

Preceding gene: 78224582

Following gene: 78224580

Centisome position: 95.21

GC content: 66.39

Gene sequence:

>1083_bases
ATGACATCTCTTTCTTCAATATCCGAGAAGGTTCACGCCGGCGAGCGAATCACCGACGAGGAGGCCCTCTTCCTCTTCGA
GTGCCGGGATCTCATCGCCGTGGGTGAGCTGGCCGCAACCGCGAACCGGCGCCGCAACGGCGACCGGGTTTTCTTCAACG
TGAACCGCCACATCAACCACACCAACATCTGTGTGAACCGCTGCTCCTTCTGCGCTTTCTACCGCACCGCCGACCAGCCG
GGGGCCTACCTCTACGACCTGGAGGAGATCCGCAACCGCGCCTTGGAGGCCCACGCCCAGGGGGCCACGGAGATCCACAT
CGTGGGCGGCCTCCACCCGGACCTCCCCTTCGACTTCTACCTGGAGATGCTCCGGACCGTGAAATCGGTCTCCCCCGAGC
TCCATCTGAAGGCCTTCACCGCCGTGGAGATCGAGTACCTCTCGAAACTGGCAGGCCTCTCCGTGGCCGAGACCCTGGCG
GAGCTGAAGGGGGCCGGGCTCGGGTCGCTGCCCGGCGGCGGGGCCGAGATCTTCGCCCCTGACGTGCGCAGCCGGCTCTG
CCCCGAGAAGATCAGCGGCGAGCGGTGGCTGGCGGTCATGGAGGAGGTCCACCGGGCGGGCTTGAAGTCCAACGCCACCA
TGCTCTTCGGTCACATCGAGAGCTACGCCGACCGGGTCGATCACATGCGCCGGCTGCGGGAGCTCCAGGACCGGACCGGA
GGGTTCCAGGTCTTCATCCCCCTGGCCTTCCAGATGGAGAACAACCCCCTGGGGCACCTGAAGCGCCCCGGCCCCGGCGG
GGTCGACGCCCTGAAAACCTTGGCGGTCGGCCGCATCTACCTGGACAACTTCGCCAACATCAAGGCCTACTGGGTGATGC
TCGGGCTGAAGACGGCCCAGGTGGCCCTCTCCTTCGGGGTGAACGACCTGGACGGCACCGTGGTGGAGGAGAAGATCGGT
CACGACGCCGGCGCCGCCACCCCCCAGACCATGGGGCGCGACGAGATCGTCACGCTGATCCGCACGGCAGGGCGGGTGCC
GGTGGAGCGGGATACGCTCTATAACGAGCTGAGGGTGTACTGA

Upstream 100 bases:

>100_bases
GGCGTTCACCATGAGCTCTTCACCCGTTGGGGAGTAAAGGCCGGGACCGGGGATTAGGGACCGGTGACCAGCAACTGATT
TTTTCATAGAGCGAGGCACC

Downstream 100 bases:

>100_bases
CCATGGATACCGTGATCGAAAAAATCGAGCAAGGGGAGGCCGTCGACCGGGCCGAGGCCTTACATCTCCTGACCAGGGCC
GACCTCCTCACCCTGGGGCG

Product: hypothetical protein

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 [H]

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MTSLSSISEKVHAGERITDEEALFLFECRDLIAVGELAATANRRRNGDRVFFNVNRHINHTNICVNRCSFCAFYRTADQP
GAYLYDLEEIRNRALEAHAQGATEIHIVGGLHPDLPFDFYLEMLRTVKSVSPELHLKAFTAVEIEYLSKLAGLSVAETLA
ELKGAGLGSLPGGGAEIFAPDVRSRLCPEKISGERWLAVMEEVHRAGLKSNATMLFGHIESYADRVDHMRRLRELQDRTG
GFQVFIPLAFQMENNPLGHLKRPGPGGVDALKTLAVGRIYLDNFANIKAYWVMLGLKTAQVALSFGVNDLDGTVVEEKIG
HDAGAATPQTMGRDEIVTLIRTAGRVPVERDTLYNELRVY

Sequences:

>Translated_360_residues
MTSLSSISEKVHAGERITDEEALFLFECRDLIAVGELAATANRRRNGDRVFFNVNRHINHTNICVNRCSFCAFYRTADQP
GAYLYDLEEIRNRALEAHAQGATEIHIVGGLHPDLPFDFYLEMLRTVKSVSPELHLKAFTAVEIEYLSKLAGLSVAETLA
ELKGAGLGSLPGGGAEIFAPDVRSRLCPEKISGERWLAVMEEVHRAGLKSNATMLFGHIESYADRVDHMRRLRELQDRTG
GFQVFIPLAFQMENNPLGHLKRPGPGGVDALKTLAVGRIYLDNFANIKAYWVMLGLKTAQVALSFGVNDLDGTVVEEKIG
HDAGAATPQTMGRDEIVTLIRTAGRVPVERDTLYNELRVY
>Mature_359_residues
TSLSSISEKVHAGERITDEEALFLFECRDLIAVGELAATANRRRNGDRVFFNVNRHINHTNICVNRCSFCAFYRTADQPG
AYLYDLEEIRNRALEAHAQGATEIHIVGGLHPDLPFDFYLEMLRTVKSVSPELHLKAFTAVEIEYLSKLAGLSVAETLAE
LKGAGLGSLPGGGAEIFAPDVRSRLCPEKISGERWLAVMEEVHRAGLKSNATMLFGHIESYADRVDHMRRLRELQDRTGG
FQVFIPLAFQMENNPLGHLKRPGPGGVDALKTLAVGRIYLDNFANIKAYWVMLGLKTAQVALSFGVNDLDGTVVEEKIGH
DAGAATPQTMGRDEIVTLIRTAGRVPVERDTLYNELRVY

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 39882; Mature: 39751

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLSSISEKVHAGERITDEEALFLFECRDLIAVGELAATANRRRNGDRVFFNVNRHINH
CCCHHHHHHHHHCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCH
TNICVNRCSFCAFYRTADQPGAYLYDLEEIRNRALEAHAQGATEIHIVGGLHPDLPFDFY
HHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHH
LEMLRTVKSVSPELHLKAFTAVEIEYLSKLAGLSVAETLAELKGAGLGSLPGGGAEIFAP
HHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEECC
DVRSRLCPEKISGERWLAVMEEVHRAGLKSNATMLFGHIESYADRVDHMRRLRELQDRTG
HHHHHCCCHHHCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCC
GFQVFIPLAFQMENNPLGHLKRPGPGGVDALKTLAVGRIYLDNFANIKAYWVMLGLKTAQ
CEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHH
VALSFGVNDLDGTVVEEKIGHDAGAATPQTMGRDEIVTLIRTAGRVPVERDTLYNELRVY
HHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
TSLSSISEKVHAGERITDEEALFLFECRDLIAVGELAATANRRRNGDRVFFNVNRHINH
CCHHHHHHHHHCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCH
TNICVNRCSFCAFYRTADQPGAYLYDLEEIRNRALEAHAQGATEIHIVGGLHPDLPFDFY
HHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHH
LEMLRTVKSVSPELHLKAFTAVEIEYLSKLAGLSVAETLAELKGAGLGSLPGGGAEIFAP
HHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEECC
DVRSRLCPEKISGERWLAVMEEVHRAGLKSNATMLFGHIESYADRVDHMRRLRELQDRTG
HHHHHCCCHHHCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCC
GFQVFIPLAFQMENNPLGHLKRPGPGGVDALKTLAVGRIYLDNFANIKAYWVMLGLKTAQ
CEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHH
VALSFGVNDLDGTVVEEKIGHDAGAATPQTMGRDEIVTLIRTAGRVPVERDTLYNELRVY
HHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]