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The map label for this gene is cofH [H]

Identifier: 78224580

GI number: 78224580

Start: 3803985

End: 3805046

Strand: Reverse

Name: cofH [H]

Synonym: Gmet_3390

Alternate gene names: 78224580

Gene position: 3805046-3803985 (Counterclockwise)

Preceding gene: 78224581

Following gene: 78224579

Centisome position: 95.19

GC content: 65.35

Gene sequence:

>1062_bases
ATGGATACCGTGATCGAAAAAATCGAGCAAGGGGAGGCCGTCGACCGGGCCGAGGCCTTACATCTCCTGACCAGGGCCGA
CCTCCTCACCCTGGGGCGCCTGGCCGACGGCATCCGCCGGCGCCTCCACCCGGAAGAGGTGGTCACCTTCGTGGTGGACC
GCAACGTGAACTACACCAACGTCTGCGAGTCCAGGTGTAAATTCTGCGCCTTCTACCGGGAGGAGGGTGCCCCCGACGCC
TACCTCCTGGCCGAGGAGGCCATCTACGCCAAGATCGCCGAGCTGGTGGAGCACGGCGGGACCCAACTCCTCATGCAGGG
GGGGCTCCACCCCCATCTGGGGATCGAGTGGTTTGAGCGGCTCTTTCGGGAGATAAAGGAGCGTTTCCCCCAGGTCCAGA
ACCACTCCCTCTCTCCGGCGGAGATTACCCAGATCGCCACGGTCTCGAAGCTCACCATTCCGGAGACGGTCAGGCGACTG
CGGGAGGCGGGGCTCGACTCCATCCCCGGCGGCGGGGCCGAGATCCTCGTGGACCGGGTTCGCAAGGAGATCTCCCCCAA
GAAGATCGGCTGGGAGGGGTGGGCCGCGGTGATGGGGGAGGCGGCCCGGCAGGGGATGGCCACCACGGCCACCATGATGT
TCGGCAGCACCGAGAAACCCGAGGATATCGCGGAGCACCTCTTCCGGGTCCGGGAGCTTCAGGCCGCCGGGGGCTCCTTC
ACCGCCTTCATCCCCTGGACCTACCAGCCCGGGAACACCGAGCTGGGGGGTGGGAGCGCCACCGGGGTCGAGTACCTGAA
GGTCCTGGCCCTTTCCCGGATCGTCCTCCATAACGTGCCGAATATCCAGGCCAGCTGGGTGACCCAGGGGGGCCAGATGG
CCCAGGTGGCCCTCTTCTTCGGCGCCAACGACCTGGGGGGGACCATGCTGGAGGAGAATGTGGTGGCCGCAGCCGGCTGC
ACCTTCCGCATGAGCCTCGATGAGATGGTGGAACTGATCCGCGAAGCCGGCTTCAGGCCTGCCCAGAGAACGACGCTTTA
TAAAATAATTCGGGAATTTTAG

Upstream 100 bases:

>100_bases
AGACCATGGGGCGCGACGAGATCGTCACGCTGATCCGCACGGCAGGGCGGGTGCCGGTGGAGCGGGATACGCTCTATAAC
GAGCTGAGGGTGTACTGACC

Downstream 100 bases:

>100_bases
AGTATTTTCCCCTCCCGACAATTCTTACGACTGGAGATTTCCTTGCCCGTTCAACCCGTTTCACCCGAGATCATAACCAC
CCCCGACGGGGTCCGCCGCC

Product: hypothetical protein

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MDTVIEKIEQGEAVDRAEALHLLTRADLLTLGRLADGIRRRLHPEEVVTFVVDRNVNYTNVCESRCKFCAFYREEGAPDA
YLLAEEAIYAKIAELVEHGGTQLLMQGGLHPHLGIEWFERLFREIKERFPQVQNHSLSPAEITQIATVSKLTIPETVRRL
REAGLDSIPGGGAEILVDRVRKEISPKKIGWEGWAAVMGEAARQGMATTATMMFGSTEKPEDIAEHLFRVRELQAAGGSF
TAFIPWTYQPGNTELGGGSATGVEYLKVLALSRIVLHNVPNIQASWVTQGGQMAQVALFFGANDLGGTMLEENVVAAAGC
TFRMSLDEMVELIREAGFRPAQRTTLYKIIREF

Sequences:

>Translated_353_residues
MDTVIEKIEQGEAVDRAEALHLLTRADLLTLGRLADGIRRRLHPEEVVTFVVDRNVNYTNVCESRCKFCAFYREEGAPDA
YLLAEEAIYAKIAELVEHGGTQLLMQGGLHPHLGIEWFERLFREIKERFPQVQNHSLSPAEITQIATVSKLTIPETVRRL
REAGLDSIPGGGAEILVDRVRKEISPKKIGWEGWAAVMGEAARQGMATTATMMFGSTEKPEDIAEHLFRVRELQAAGGSF
TAFIPWTYQPGNTELGGGSATGVEYLKVLALSRIVLHNVPNIQASWVTQGGQMAQVALFFGANDLGGTMLEENVVAAAGC
TFRMSLDEMVELIREAGFRPAQRTTLYKIIREF
>Mature_353_residues
MDTVIEKIEQGEAVDRAEALHLLTRADLLTLGRLADGIRRRLHPEEVVTFVVDRNVNYTNVCESRCKFCAFYREEGAPDA
YLLAEEAIYAKIAELVEHGGTQLLMQGGLHPHLGIEWFERLFREIKERFPQVQNHSLSPAEITQIATVSKLTIPETVRRL
REAGLDSIPGGGAEILVDRVRKEISPKKIGWEGWAAVMGEAARQGMATTATMMFGSTEKPEDIAEHLFRVRELQAAGGSF
TAFIPWTYQPGNTELGGGSATGVEYLKVLALSRIVLHNVPNIQASWVTQGGQMAQVALFFGANDLGGTMLEENVVAAAGC
TFRMSLDEMVELIREAGFRPAQRTTLYKIIREF

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 39117; Mature: 39117

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTVIEKIEQGEAVDRAEALHLLTRADLLTLGRLADGIRRRLHPEEVVTFVVDRNVNYTN
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHH
VCESRCKFCAFYREEGAPDAYLLAEEAIYAKIAELVEHGGTQLLMQGGLHPHLGIEWFER
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHH
LFREIKERFPQVQNHSLSPAEITQIATVSKLTIPETVRRLREAGLDSIPGGGAEILVDRV
HHHHHHHHCCHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHH
RKEISPKKIGWEGWAAVMGEAARQGMATTATMMFGSTEKPEDIAEHLFRVRELQAAGGSF
HHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCE
TAFIPWTYQPGNTELGGGSATGVEYLKVLALSRIVLHNVPNIQASWVTQGGQMAQVALFF
EEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCEEEEEEE
GANDLGGTMLEENVVAAAGCTFRMSLDEMVELIREAGFRPAQRTTLYKIIREF
CCCCCCCHHHHHCHHHHCCCEEEECHHHHHHHHHHCCCCCHHHHHHHHHHHHC
>Mature Secondary Structure
MDTVIEKIEQGEAVDRAEALHLLTRADLLTLGRLADGIRRRLHPEEVVTFVVDRNVNYTN
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHH
VCESRCKFCAFYREEGAPDAYLLAEEAIYAKIAELVEHGGTQLLMQGGLHPHLGIEWFER
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHH
LFREIKERFPQVQNHSLSPAEITQIATVSKLTIPETVRRLREAGLDSIPGGGAEILVDRV
HHHHHHHHCCHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHH
RKEISPKKIGWEGWAAVMGEAARQGMATTATMMFGSTEKPEDIAEHLFRVRELQAAGGSF
HHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCE
TAFIPWTYQPGNTELGGGSATGVEYLKVLALSRIVLHNVPNIQASWVTQGGQMAQVALFF
EEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCEEEEEEE
GANDLGGTMLEENVVAAAGCTFRMSLDEMVELIREAGFRPAQRTTLYKIIREF
CCCCCCCHHHHHCHHHHCCCEEEECHHHHHHHHHHCCCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]