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The map label for this gene is epsF [H]

Identifier: 78224556

GI number: 78224556

Start: 3783440

End: 3784651

Strand: Direct

Name: epsF [H]

Synonym: Gmet_3366

Alternate gene names: 78224556

Gene position: 3783440-3784651 (Clockwise)

Preceding gene: 78224555

Following gene: 78224557

Centisome position: 94.65

GC content: 66.25

Gene sequence:

>1212_bases
ATGCCGACCTTCCGGTATAGCGCCTATACGGCCGAAGGCCGCGACACCGCCGGCACCCTGGAGGCGGAAAACCTCAAGGA
GGCCCGCCAGCGGCTCAAGCGGGACGGCCTCTTCCCCCGAGAGATCTCGCCGGCCGAAGATGAGGGAGCGGCACCGGGAC
GCCGCCTCTTCGGGGGGCACACCGGGCTTGCGGAGATCGCCCTCATGACCCGGCGGCTGGCAACCCTCGTGGGCTCCCAG
GTGCCGATCTTCGAGGCGGTCACCACCCTCGGTGAGCAGGAGGAGCCGGGAGAGCTGAAAAAGGCCCTGGGGCGCATCCG
GGAACGGCTGGCCGAGGGTGCGAGCTTGGCCAAGGCTCTCTCCCTGGAGCCGGGGCTCTTCAGCGAGAGCTACGTGGCCA
TGGTGGCGGCGGGCGAGGCAAGCGGCTCCCTCGAAACGGTGCTGGAAAAGCTTGCCCTCTTTCTGGAGGAGCAGCGGGCC
ATCCGAAGCAAGGTGACGGCCTCCCTCGCCTACCCGCTCCTCATGATCGTCGTGGGTGGGGGGGTGATGCTCTTTCTTCT
CACCTTCGTCATCCCCAAGATCGTCACCATCTTCGAGGACAACAAGGCTGCCCTCCCCCTCATCACCATTGCGCTCATCA
AGACGAGTGCCTTCCTGCGCCACTTCTGGTGGGCACTTCTGGGGCTCGCCGCCGGCGGAGTATTCCTCTACCGCCGCCTC
GCGACGCAGGAAGGGTTCCGGCTCCGGCGGGACCGGTGGCTCTTGGGGCTCCCCGTGGTGGGGGGACTCATCCGGCACCT
GATCCTCTCCCGCTTCGCCAAGGTCCTAGGGCTTCTGCTGGCCAGTGGCGTGCCGGTCATCAAGGCCCTGGAGATCACCG
CCCAGGTCCTGGTGAACCGGGTCTACCGGGGGTCTCTCCACACTATCGCGGCGGAACTGGCCGAGGGGGGGAACCTCTCG
GGGGCGCTACGCAAAGGAACGCTCTTTCCGCCACTCCTGATCCACATGGTGGCGGTGGGAGAAAAGGGGGGAGAGCTGGA
AGAGATGCTGGCCAAGGCGGGAAATGCCTTCGAGCGAGAGTTTGACACCGCCGTTTCGGGGCTCATGGCGCTGCTGGAGC
CGCTCCTGGTCCTCGCCATGGGGCTTGCCGTGGGGCTCGTGGTCCTGGCAGTGCTCCTCCCCATATTCGAATTGAACCAA
CTGGTCCGATAA

Upstream 100 bases:

>100_bases
GTCTCCCGCGGCATGCGGACCCTGCGGGATGATGGCCTTGCCAAGGCGGCGGCCGGCATCACCACCATCGAGGAGGTTCT
GCGGGTGACCCAGGAGGAGT

Downstream 100 bases:

>100_bases
TCCTGACAGGAGGTCTTCCCCAATGAACCGGAAACCCATGAACAACCGCGGCTTCACCCTCATCGAAATCATGGTGGTCA
TCGCCATCCTGGCGCTCCTG

Product: general secretion pathway protein F

Products: NA

Alternate protein names: Cholera toxin secretion protein epsF [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MPTFRYSAYTAEGRDTAGTLEAENLKEARQRLKRDGLFPREISPAEDEGAAPGRRLFGGHTGLAEIALMTRRLATLVGSQ
VPIFEAVTTLGEQEEPGELKKALGRIRERLAEGASLAKALSLEPGLFSESYVAMVAAGEASGSLETVLEKLALFLEEQRA
IRSKVTASLAYPLLMIVVGGGVMLFLLTFVIPKIVTIFEDNKAALPLITIALIKTSAFLRHFWWALLGLAAGGVFLYRRL
ATQEGFRLRRDRWLLGLPVVGGLIRHLILSRFAKVLGLLLASGVPVIKALEITAQVLVNRVYRGSLHTIAAELAEGGNLS
GALRKGTLFPPLLIHMVAVGEKGGELEEMLAKAGNAFEREFDTAVSGLMALLEPLLVLAMGLAVGLVVLAVLLPIFELNQ
LVR

Sequences:

>Translated_403_residues
MPTFRYSAYTAEGRDTAGTLEAENLKEARQRLKRDGLFPREISPAEDEGAAPGRRLFGGHTGLAEIALMTRRLATLVGSQ
VPIFEAVTTLGEQEEPGELKKALGRIRERLAEGASLAKALSLEPGLFSESYVAMVAAGEASGSLETVLEKLALFLEEQRA
IRSKVTASLAYPLLMIVVGGGVMLFLLTFVIPKIVTIFEDNKAALPLITIALIKTSAFLRHFWWALLGLAAGGVFLYRRL
ATQEGFRLRRDRWLLGLPVVGGLIRHLILSRFAKVLGLLLASGVPVIKALEITAQVLVNRVYRGSLHTIAAELAEGGNLS
GALRKGTLFPPLLIHMVAVGEKGGELEEMLAKAGNAFEREFDTAVSGLMALLEPLLVLAMGLAVGLVVLAVLLPIFELNQ
LVR
>Mature_402_residues
PTFRYSAYTAEGRDTAGTLEAENLKEARQRLKRDGLFPREISPAEDEGAAPGRRLFGGHTGLAEIALMTRRLATLVGSQV
PIFEAVTTLGEQEEPGELKKALGRIRERLAEGASLAKALSLEPGLFSESYVAMVAAGEASGSLETVLEKLALFLEEQRAI
RSKVTASLAYPLLMIVVGGGVMLFLLTFVIPKIVTIFEDNKAALPLITIALIKTSAFLRHFWWALLGLAAGGVFLYRRLA
TQEGFRLRRDRWLLGLPVVGGLIRHLILSRFAKVLGLLLASGVPVIKALEITAQVLVNRVYRGSLHTIAAELAEGGNLSG
ALRKGTLFPPLLIHMVAVGEKGGELEEMLAKAGNAFEREFDTAVSGLMALLEPLLVLAMGLAVGLVVLAVLLPIFELNQL
VR

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=403, Percent_Identity=33.7468982630273, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI1786295, Length=399, Percent_Identity=26.3157894736842, Blast_Score=108, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR011850
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 43479; Mature: 43348

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS00874 T2SP_F

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTFRYSAYTAEGRDTAGTLEAENLKEARQRLKRDGLFPREISPAEDEGAAPGRRLFGGH
CCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCC
TGLAEIALMTRRLATLVGSQVPIFEAVTTLGEQEEPGELKKALGRIRERLAEGASLAKAL
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SLEPGLFSESYVAMVAAGEASGSLETVLEKLALFLEEQRAIRSKVTASLAYPLLMIVVGG
CCCCCCCCCHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVMLFLLTFVIPKIVTIFEDNKAALPLITIALIKTSAFLRHFWWALLGLAAGGVFLYRRL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATQEGFRLRRDRWLLGLPVVGGLIRHLILSRFAKVLGLLLASGVPVIKALEITAQVLVNR
HHHHHHHHHHCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
VYRGSLHTIAAELAEGGNLSGALRKGTLFPPLLIHMVAVGEKGGELEEMLAKAGNAFERE
HHHHHHHHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHH
FDTAVSGLMALLEPLLVLAMGLAVGLVVLAVLLPIFELNQLVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PTFRYSAYTAEGRDTAGTLEAENLKEARQRLKRDGLFPREISPAEDEGAAPGRRLFGGH
CCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCC
TGLAEIALMTRRLATLVGSQVPIFEAVTTLGEQEEPGELKKALGRIRERLAEGASLAKAL
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SLEPGLFSESYVAMVAAGEASGSLETVLEKLALFLEEQRAIRSKVTASLAYPLLMIVVGG
CCCCCCCCCHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVMLFLLTFVIPKIVTIFEDNKAALPLITIALIKTSAFLRHFWWALLGLAAGGVFLYRRL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATQEGFRLRRDRWLLGLPVVGGLIRHLILSRFAKVLGLLLASGVPVIKALEITAQVLVNR
HHHHHHHHHHCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
VYRGSLHTIAAELAEGGNLSGALRKGTLFPPLLIHMVAVGEKGGELEEMLAKAGNAFERE
HHHHHHHHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHH
FDTAVSGLMALLEPLLVLAMGLAVGLVVLAVLLPIFELNQLVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]