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The map label for this gene is epsE [H]

Identifier: 78224555

GI number: 78224555

Start: 3781897

End: 3783459

Strand: Direct

Name: epsE [H]

Synonym: Gmet_3365

Alternate gene names: 78224555

Gene position: 3781897-3783459 (Clockwise)

Preceding gene: 78224554

Following gene: 78224556

Centisome position: 94.61

GC content: 66.28

Gene sequence:

>1563_bases
ATGACCGATACCCACGACGTGGAACGCATAGCCGAAAGGCTCGGTATCCCTTTCACGGCCGAAATCGAGGAAGGTGAAGC
CGACTTGACGCTCCTGGCCCGGCTTCCCCTCGCTTTCGCCCGGGGGCGGCTCGTCCTGCCCTTGCGGGAGCGAGACGGGA
GACTGATGGTGGCGGCCGGGAACCCGGCCGACCTGGCCGCCGTTGACGAGGTGCGCGGGGTCTACGGCCTGCCGGTGGAG
CTTGTGGCGGCGCCGCAAAAGACGGTCCTCGACGCGGTGAACCGCCTCTATGGCCGCCTCGGGAGCACGGCCCAGGAGGT
GGTGGAGGAACTGGTTGGAGAAGACCTCTCTGTCATCGCCACCGAGCTCTCGGAACCCAAGGATCTCCTGGATCTGACCG
ACGAGGCCCCGGTTATCCGGCTCCTCAACTCCATCCTCTCCGAGGCGGTGAAGGAGCGGGCCAGCGACATCCACATCGAG
CCCTACGAGCGGGAGCTGGAGGTCCGGTTCAGGATCGACGGCATCCTCTACAAAAAGCTCGCCCCCCCCAAGGTGGTGCA
GGAGGCCCTTGCCTCCCGGGTGAAGATCATGGCAGGCCTCAACATCGCCGAAAAACGCCTCCCCCAGGACGGCCGCATCC
GGGTCATCGTGGCAGGGCGCGACGTGGACATCCGGGTCTCCATCATCCCCACCTTTTTCGGCGAGCGGACCGTGCTCCGG
CTCCTGGACAAGCAGAAGGGGATCATCTCCCTGGCCGACATCGGCATGGAAGAGCGGGGGGTCGCCGCCATGGAGCGGCT
CCTGTCACGCACCAGCGGCATCATCCTCGTCACCGGCCCCACGGGGAGCGGCAAGTCCACCACCCTCTACGCGGCCCTGA
ACCGACTCAACTCCCCCGAGAAGAACATCATCACCATCGAGGACCCCATCGAATACCAGATCAAGGGGATCGGCCAGATC
CAGGTGAACCCCAAGATCGACCTCACCTTTGCCCAGGGGCTCCGGGCCATCCTCCGCCAGGACCCGGACATCGTCATGGT
GGGGGAGATCCGGGACGCCGAGACTGCGGAGATCGCCATGCAGGCCTCCCTCACGGGGCACCTGGTCCTCTCCACCCTCC
ACACCAACGATTCGGCCACGGCCATCGCCCGGCTCGTGGACATGGGGATCGAACCGTTCATGGTGGCGTCGTCCCTTTCG
GCGGTCCTGGCCCAGCGTCTCGTGCGTCGCATCTGCCCCCACTGCCGGGAGCCCTATACGCCCGAGCGCCAGTACGCCGG
CATCACTCTCCCCGCCACCCTCTACCGGGGACGGGGATGCGAGAAGTGCTACGGACTCGGCACCTGGGGACGGGTCGGCA
TCTACGAGCTCCTCCCCGTGGACGGGGAGATCTGTTCCATGATCATCCGGCGGGAACCGGCGGGAACCATCAAGGAGTAC
GCCGTCTCCCGCGGCATGCGGACCCTGCGGGATGATGGCCTTGCCAAGGCGGCGGCCGGCATCACCACCATCGAGGAGGT
TCTGCGGGTGACCCAGGAGGAGTATGCCGACCTTCCGGTATAG

Upstream 100 bases:

>100_bases
AAATGGCTGAGGTGTCGAACCTTCAGAAGGGAACGTTCGACAACGCGGTGAGGATGGATATCCCCTTCAGCCTCGACCGG
GAAGAACTGAAGACGAAGCA

Downstream 100 bases:

>100_bases
CGCCTATACGGCCGAAGGCCGCGACACCGCCGGCACCCTGGAGGCGGAAAACCTCAAGGAGGCCCGCCAGCGGCTCAAGC
GGGACGGCCTCTTCCCCCGA

Product: type II secretion system protein E

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MTDTHDVERIAERLGIPFTAEIEEGEADLTLLARLPLAFARGRLVLPLRERDGRLMVAAGNPADLAAVDEVRGVYGLPVE
LVAAPQKTVLDAVNRLYGRLGSTAQEVVEELVGEDLSVIATELSEPKDLLDLTDEAPVIRLLNSILSEAVKERASDIHIE
PYERELEVRFRIDGILYKKLAPPKVVQEALASRVKIMAGLNIAEKRLPQDGRIRVIVAGRDVDIRVSIIPTFFGERTVLR
LLDKQKGIISLADIGMEERGVAAMERLLSRTSGIILVTGPTGSGKSTTLYAALNRLNSPEKNIITIEDPIEYQIKGIGQI
QVNPKIDLTFAQGLRAILRQDPDIVMVGEIRDAETAEIAMQASLTGHLVLSTLHTNDSATAIARLVDMGIEPFMVASSLS
AVLAQRLVRRICPHCREPYTPERQYAGITLPATLYRGRGCEKCYGLGTWGRVGIYELLPVDGEICSMIIRREPAGTIKEY
AVSRGMRTLRDDGLAKAAAGITTIEEVLRVTQEEYADLPV

Sequences:

>Translated_520_residues
MTDTHDVERIAERLGIPFTAEIEEGEADLTLLARLPLAFARGRLVLPLRERDGRLMVAAGNPADLAAVDEVRGVYGLPVE
LVAAPQKTVLDAVNRLYGRLGSTAQEVVEELVGEDLSVIATELSEPKDLLDLTDEAPVIRLLNSILSEAVKERASDIHIE
PYERELEVRFRIDGILYKKLAPPKVVQEALASRVKIMAGLNIAEKRLPQDGRIRVIVAGRDVDIRVSIIPTFFGERTVLR
LLDKQKGIISLADIGMEERGVAAMERLLSRTSGIILVTGPTGSGKSTTLYAALNRLNSPEKNIITIEDPIEYQIKGIGQI
QVNPKIDLTFAQGLRAILRQDPDIVMVGEIRDAETAEIAMQASLTGHLVLSTLHTNDSATAIARLVDMGIEPFMVASSLS
AVLAQRLVRRICPHCREPYTPERQYAGITLPATLYRGRGCEKCYGLGTWGRVGIYELLPVDGEICSMIIRREPAGTIKEY
AVSRGMRTLRDDGLAKAAAGITTIEEVLRVTQEEYADLPV
>Mature_519_residues
TDTHDVERIAERLGIPFTAEIEEGEADLTLLARLPLAFARGRLVLPLRERDGRLMVAAGNPADLAAVDEVRGVYGLPVEL
VAAPQKTVLDAVNRLYGRLGSTAQEVVEELVGEDLSVIATELSEPKDLLDLTDEAPVIRLLNSILSEAVKERASDIHIEP
YERELEVRFRIDGILYKKLAPPKVVQEALASRVKIMAGLNIAEKRLPQDGRIRVIVAGRDVDIRVSIIPTFFGERTVLRL
LDKQKGIISLADIGMEERGVAAMERLLSRTSGIILVTGPTGSGKSTTLYAALNRLNSPEKNIITIEDPIEYQIKGIGQIQ
VNPKIDLTFAQGLRAILRQDPDIVMVGEIRDAETAEIAMQASLTGHLVLSTLHTNDSATAIARLVDMGIEPFMVASSLSA
VLAQRLVRRICPHCREPYTPERQYAGITLPATLYRGRGCEKCYGLGTWGRVGIYELLPVDGEICSMIIRREPAGTIKEYA
VSRGMRTLRDDGLAKAAAGITTIEEVLRVTQEEYADLPV

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=406, Percent_Identity=51.9704433497537, Blast_Score=410, Evalue=1e-115,
Organism=Escherichia coli, GI1786296, Length=381, Percent_Identity=44.0944881889764, Blast_Score=325, Evalue=3e-90,
Organism=Escherichia coli, GI87082188, Length=279, Percent_Identity=34.7670250896057, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 57004; Mature: 56873

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTHDVERIAERLGIPFTAEIEEGEADLTLLARLPLAFARGRLVLPLRERDGRLMVAAG
CCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCEEEEEEC
NPADLAAVDEVRGVYGLPVELVAAPQKTVLDAVNRLYGRLGSTAQEVVEELVGEDLSVIA
CCCCHHHHHHHCCCCCCCHHEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHH
TELSEPKDLLDLTDEAPVIRLLNSILSEAVKERASDIHIEPYERELEVRFRIDGILYKKL
HHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCEEHHCC
APPKVVQEALASRVKIMAGLNIAEKRLPQDGRIRVIVAGRDVDIRVSIIPTFFGERTVLR
CCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEEECCCEEEEEEEEECCCCHHHHHH
LLDKQKGIISLADIGMEERGVAAMERLLSRTSGIILVTGPTGSGKSTTLYAALNRLNSPE
HHHHCCCEEEEHHCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHCCCCC
KNIITIEDPIEYQIKGIGQIQVNPKIDLTFAQGLRAILRQDPDIVMVGEIRDAETAEIAM
CCEEEEECCCEEEEEECEEEEECCCEEEHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHH
QASLTGHLVLSTLHTNDSATAIARLVDMGIEPFMVASSLSAVLAQRLVRRICPHCREPYT
HHCCCEEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PERQYAGITLPATLYRGRGCEKCYGLGTWGRVGIYELLPVDGEICSMIIRREPAGTIKEY
CCHHCCCEEECHHHHCCCCCHHHHCCCCCCCCCCCEEECCCHHHHHHHHHCCCCCHHHHH
AVSRGMRTLRDDGLAKAAAGITTIEEVLRVTQEEYADLPV
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TDTHDVERIAERLGIPFTAEIEEGEADLTLLARLPLAFARGRLVLPLRERDGRLMVAAG
CCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCEEEEEEC
NPADLAAVDEVRGVYGLPVELVAAPQKTVLDAVNRLYGRLGSTAQEVVEELVGEDLSVIA
CCCCHHHHHHHCCCCCCCHHEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHH
TELSEPKDLLDLTDEAPVIRLLNSILSEAVKERASDIHIEPYERELEVRFRIDGILYKKL
HHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCEEHHCC
APPKVVQEALASRVKIMAGLNIAEKRLPQDGRIRVIVAGRDVDIRVSIIPTFFGERTVLR
CCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEEECCCEEEEEEEEECCCCHHHHHH
LLDKQKGIISLADIGMEERGVAAMERLLSRTSGIILVTGPTGSGKSTTLYAALNRLNSPE
HHHHCCCEEEEHHCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHCCCCC
KNIITIEDPIEYQIKGIGQIQVNPKIDLTFAQGLRAILRQDPDIVMVGEIRDAETAEIAM
CCEEEEECCCEEEEEECEEEEECCCEEEHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHH
QASLTGHLVLSTLHTNDSATAIARLVDMGIEPFMVASSLSAVLAQRLVRRICPHCREPYT
HHCCCEEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PERQYAGITLPATLYRGRGCEKCYGLGTWGRVGIYELLPVDGEICSMIIRREPAGTIKEY
CCHHCCCEEECHHHHCCCCCHHHHCCCCCCCCCCCEEECCCHHHHHHHHHCCCCCHHHHH
AVSRGMRTLRDDGLAKAAAGITTIEEVLRVTQEEYADLPV
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]