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The map label for this gene is nuoB [H]

Identifier: 78224544

GI number: 78224544

Start: 3771704

End: 3772216

Strand: Reverse

Name: nuoB [H]

Synonym: Gmet_3354

Alternate gene names: 78224544

Gene position: 3772216-3771704 (Counterclockwise)

Preceding gene: 78224545

Following gene: 78224543

Centisome position: 94.37

GC content: 53.61

Gene sequence:

>513_bases
ATGGGAGTAGATCAGCCGTTGGGAGATAGCTTCATTACAGCGTCCTTGGACGGTCTCGTCAACTGGGCGAGGAAGTCCTC
CATTTGGCCGATGACCTTCGGTCTTGCCTGCTGTGCCATCGAGATGATGGCTACCGGGGCATCCCATAACGACCTTGACC
GTTTTGGTATTATTTTCCGTGCTTCGCCAAGGCAGGCCGACTGCATCATTATTGCTGGAACGGTCACCAAAAAGATGCTG
CCCGTAATCCAGACCGTTTACGAGCAAATGCCTGAGCCGAAGTGGGTGGTCGCTATGGGGGCCTGTGCCTGTTCGGGTGG
GGTTTTTGATACGTATAGTGTCGTGCAGGGGATTGATGAGGCGCTTCCGGTAGATGTGTATATCCCTGGTTGCCCTCCCC
GGCCGGAGGCATTGCTTTATGGTCTTATCAAGCTTCAGGATAAAATCATGAAGGATAAGAACTCCTTTGGTTCTACTATC
GGTCTTGGCCAGCGACTCGAGTCGGCGGCGTGA

Upstream 100 bases:

>100_bases
TCTTTAAGAGGTTGGGGATGTTCGGCGTGATGGAGATGGGGGTCTTTATCGTTATACTCTTTGTTGGTTACATCTATGTA
TGGAAAAAAGGAGCCTTGGA

Downstream 100 bases:

>100_bases
CCATATCAACTAGGTAAGGGAGCCAACGGTTATGGCTGAAACTAATCGCGCAGTAATAAAGCTCAGGGAAAAATTTTCCG
CTTCCGTGCTTGATGTGAAA

Product: NADH dehydrogenase (ubiquinone), 20 kDa subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 170; Mature: 169

Protein sequence:

>170_residues
MGVDQPLGDSFITASLDGLVNWARKSSIWPMTFGLACCAIEMMATGASHNDLDRFGIIFRASPRQADCIIIAGTVTKKML
PVIQTVYEQMPEPKWVVAMGACACSGGVFDTYSVVQGIDEALPVDVYIPGCPPRPEALLYGLIKLQDKIMKDKNSFGSTI
GLGQRLESAA

Sequences:

>Translated_170_residues
MGVDQPLGDSFITASLDGLVNWARKSSIWPMTFGLACCAIEMMATGASHNDLDRFGIIFRASPRQADCIIIAGTVTKKML
PVIQTVYEQMPEPKWVVAMGACACSGGVFDTYSVVQGIDEALPVDVYIPGCPPRPEALLYGLIKLQDKIMKDKNSFGSTI
GLGQRLESAA
>Mature_169_residues
GVDQPLGDSFITASLDGLVNWARKSSIWPMTFGLACCAIEMMATGASHNDLDRFGIIFRASPRQADCIIIAGTVTKKMLP
VIQTVYEQMPEPKWVVAMGACACSGGVFDTYSVVQGIDEALPVDVYIPGCPPRPEALLYGLIKLQDKIMKDKNSFGSTIG
LGQRLESAA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=145, Percent_Identity=60.6896551724138, Blast_Score=206, Evalue=1e-53,
Organism=Escherichia coli, GI1788624, Length=160, Percent_Identity=52.5, Blast_Score=189, Evalue=7e-50,
Organism=Escherichia coli, GI1789074, Length=110, Percent_Identity=46.3636363636364, Blast_Score=98, Evalue=2e-22,
Organism=Escherichia coli, GI1788834, Length=121, Percent_Identity=43.801652892562, Blast_Score=91, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17509685, Length=146, Percent_Identity=55.4794520547945, Blast_Score=190, Evalue=2e-49,
Organism=Drosophila melanogaster, GI18859983, Length=154, Percent_Identity=56.4935064935065, Blast_Score=200, Evalue=4e-52,
Organism=Drosophila melanogaster, GI24642371, Length=154, Percent_Identity=56.4935064935065, Blast_Score=200, Evalue=4e-52,
Organism=Drosophila melanogaster, GI24651058, Length=152, Percent_Identity=54.6052631578947, Blast_Score=196, Evalue=8e-51,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 18291; Mature: 18159

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
8.2 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVDQPLGDSFITASLDGLVNWARKSSIWPMTFGLACCAIEMMATGASHNDLDRFGIIFR
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHEEEEE
ASPRQADCIIIAGTVTKKMLPVIQTVYEQMPEPKWVVAMGACACSGGVFDTYSVVQGIDE
CCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHCCCCCHHHHHHHHHHH
ALPVDVYIPGCPPRPEALLYGLIKLQDKIMKDKNSFGSTIGLGQRLESAA
HCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure 
GVDQPLGDSFITASLDGLVNWARKSSIWPMTFGLACCAIEMMATGASHNDLDRFGIIFR
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHEEEEE
ASPRQADCIIIAGTVTKKMLPVIQTVYEQMPEPKWVVAMGACACSGGVFDTYSVVQGIDE
CCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHCCCCCHHHHHHHHHHH
ALPVDVYIPGCPPRPEALLYGLIKLQDKIMKDKNSFGSTIGLGQRLESAA
HCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA