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The map label for this gene is nuoF2 [H]

Identifier: 78224540

GI number: 78224540

Start: 3767589

End: 3769364

Strand: Reverse

Name: nuoF2 [H]

Synonym: Gmet_3350

Alternate gene names: 78224540

Gene position: 3769364-3767589 (Counterclockwise)

Preceding gene: 78224541

Following gene: 78224539

Centisome position: 94.29

GC content: 52.87

Gene sequence:

>1776_bases
ATGGGCGAAGCTATAAAGATTCTGATTTGTCAGGGGACAGGCGGCGTATCGGCCGGTGCAAAAAAGGTCGAGGCCGAATT
TAATCGGATAATTTCCGAAAGAGGCGTCAACGCCGTTGTGGGCAAGCGTTGCGACATTATTAAGACTGGCTGCCGCGGAC
TCTGTGCAAACGATGTTCTCGTCGATATCGTGGATCCTGAGCTTGGACGGGTCACCTATGATTTTGTGCAGCCAGAAGAA
GTAGCGCAGCTCGTTGATGAACACATCATCAAGCGCACGCCTTTGGAAAAGCGAAAGGCAAAACCTTATTACAATACATT
TGTCGATCAACAAATGCGGATTGTTATGACCGGTTGCGGTCAGATTGATCCCGAGAGCCTTGACGCCTATCTGGCAGAAG
ATGGATTCAAGGCCATCGAAAAATGCGTGAAAGAGATGACTCCGGCTGCGGTTATCGATGAGGTCAAGAAGGCGGGTCTG
CGCGGCCGTGGGGGGGGGGGCTTTCCCACCGGCATGAAGTGGTCCTTTTGTGCTGCTACACCCGGGAAGCTTAAATATTT
GATCTGTAATGCTGACGAAGGGGATCCCGGGGCCTTCATGGACCGTTCGATCCTTGAGGGTGACCCCTACTGCATCATCG
AGGGGATGATGATTGCGGCGTATGCGATCGGTTGCTCTAAGGGGTACGTTTACGTACGCGCAGAGTATCCGCTGGCGATT
GAGCGCCTCCAAAAGGCCCTTGATGTATGCCGTGAGAAAGGCTATCTCGGGCACAATATACAAGGTTGGGGCTTTGACTT
TGACCTGATTATCAAAAAGGGTGCCGGTGCCTTCGTGTGCGGTGAAGAGACTGCGCTCATGGCTTCCATCGAAGGTGAAC
GAGGCATGCCACGCCCCCGGCCACCGTTCCCTGCGGTCAAGGGCTTGTGGGCCAAGCCGACCAACATCAATAACGTTGAG
ACGTTTGCCAACGTCCGTCACATAATCAACAAGGGAGCTGAGTGGTATGCCTCCCTCGGAACTGACACGACCAAGGGGAC
CAAAATTTTTGCCGTCACTGGCAAGGTAAAACACACGGGCCTTGTTGAGGTTCCTGCAGGCATGACAGTTCGCGATGTTA
TTTATAATGTCTGCGGTGGGATTGCGAATAATCGCAAATTCAAGGCGGTACAGGCGGGTGGGCCCTCTGGTGGCTGTATC
CCTCCTGAAGTGCTTGATACTCCCGTTGACTACGATTCATTGATTAAGGCCGGAGCCATGATGGGTTCGGGTGGTCTGGT
CGTCATGGACGAGACAACCTGTATGGTCGACGTTGCCCGCTTCTTCCTTTCGTTCACCAGAATGGAGTCTTGCGGGAAAT
GTGTCCCCTGCCGGATTGGCCTCAAGGTTATGCTTGACATTCTGGAGAGAATTACCGCAGGCCGCGGCCAGGCGGGCGAC
ATTGAGACTCTTCTGGAGATGGGCAGTACCATCAAGAAGGCTTCTTTATGTGGCCTCGGCCAAACAGCGCCGAACCCTAT
TCTCTCGACCATTCGCTATTTCCGGGAAGAGTACGAAGCCCACATCACCGATAAACGCTGCCCGTCAAACTGCTGCAAGG
AACTACTCCTGTGGCAGGTAATAGAGGAAAAGTGCGTTAAGTGCGGTGCGTGCTTCAAAGCGTGTCCGGTAGACGCAATT
GTCTGGGAAAAGGGACAGGTTGCCTATTTGGACAAGGAAAAGTGTACAAAATGTAAGTCGTGTTATGACGCCTGTCGGTT
CATGGCGATTGAGTAA

Upstream 100 bases:

>100_bases
AAAATGACAGTGCAAAAGGTTGAAGAGATTATCGCTGAGTATAATCAGCGGCCGATGAAGTAGGATCGATCCAACCATTC
ACATCAGAGGATAGAGCGTT

Downstream 100 bases:

>100_bases
CAGCTCGATGAGCTAACTATCCGAGGTTATACAGAGGTCAAGATGGTAAATCTCACGATTGACGGAAAACAGGTAACGGT
ACCCAAGGACGCTACCATTT

Product: NADH dehydrogenase I subunit F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 591; Mature: 590

Protein sequence:

>591_residues
MGEAIKILICQGTGGVSAGAKKVEAEFNRIISERGVNAVVGKRCDIIKTGCRGLCANDVLVDIVDPELGRVTYDFVQPEE
VAQLVDEHIIKRTPLEKRKAKPYYNTFVDQQMRIVMTGCGQIDPESLDAYLAEDGFKAIEKCVKEMTPAAVIDEVKKAGL
RGRGGGGFPTGMKWSFCAATPGKLKYLICNADEGDPGAFMDRSILEGDPYCIIEGMMIAAYAIGCSKGYVYVRAEYPLAI
ERLQKALDVCREKGYLGHNIQGWGFDFDLIIKKGAGAFVCGEETALMASIEGERGMPRPRPPFPAVKGLWAKPTNINNVE
TFANVRHIINKGAEWYASLGTDTTKGTKIFAVTGKVKHTGLVEVPAGMTVRDVIYNVCGGIANNRKFKAVQAGGPSGGCI
PPEVLDTPVDYDSLIKAGAMMGSGGLVVMDETTCMVDVARFFLSFTRMESCGKCVPCRIGLKVMLDILERITAGRGQAGD
IETLLEMGSTIKKASLCGLGQTAPNPILSTIRYFREEYEAHITDKRCPSNCCKELLLWQVIEEKCVKCGACFKACPVDAI
VWEKGQVAYLDKEKCTKCKSCYDACRFMAIE

Sequences:

>Translated_591_residues
MGEAIKILICQGTGGVSAGAKKVEAEFNRIISERGVNAVVGKRCDIIKTGCRGLCANDVLVDIVDPELGRVTYDFVQPEE
VAQLVDEHIIKRTPLEKRKAKPYYNTFVDQQMRIVMTGCGQIDPESLDAYLAEDGFKAIEKCVKEMTPAAVIDEVKKAGL
RGRGGGGFPTGMKWSFCAATPGKLKYLICNADEGDPGAFMDRSILEGDPYCIIEGMMIAAYAIGCSKGYVYVRAEYPLAI
ERLQKALDVCREKGYLGHNIQGWGFDFDLIIKKGAGAFVCGEETALMASIEGERGMPRPRPPFPAVKGLWAKPTNINNVE
TFANVRHIINKGAEWYASLGTDTTKGTKIFAVTGKVKHTGLVEVPAGMTVRDVIYNVCGGIANNRKFKAVQAGGPSGGCI
PPEVLDTPVDYDSLIKAGAMMGSGGLVVMDETTCMVDVARFFLSFTRMESCGKCVPCRIGLKVMLDILERITAGRGQAGD
IETLLEMGSTIKKASLCGLGQTAPNPILSTIRYFREEYEAHITDKRCPSNCCKELLLWQVIEEKCVKCGACFKACPVDAI
VWEKGQVAYLDKEKCTKCKSCYDACRFMAIE
>Mature_590_residues
GEAIKILICQGTGGVSAGAKKVEAEFNRIISERGVNAVVGKRCDIIKTGCRGLCANDVLVDIVDPELGRVTYDFVQPEEV
AQLVDEHIIKRTPLEKRKAKPYYNTFVDQQMRIVMTGCGQIDPESLDAYLAEDGFKAIEKCVKEMTPAAVIDEVKKAGLR
GRGGGGFPTGMKWSFCAATPGKLKYLICNADEGDPGAFMDRSILEGDPYCIIEGMMIAAYAIGCSKGYVYVRAEYPLAIE
RLQKALDVCREKGYLGHNIQGWGFDFDLIIKKGAGAFVCGEETALMASIEGERGMPRPRPPFPAVKGLWAKPTNINNVET
FANVRHIINKGAEWYASLGTDTTKGTKIFAVTGKVKHTGLVEVPAGMTVRDVIYNVCGGIANNRKFKAVQAGGPSGGCIP
PEVLDTPVDYDSLIKAGAMMGSGGLVVMDETTCMVDVARFFLSFTRMESCGKCVPCRIGLKVMLDILERITAGRGQAGDI
ETLLEMGSTIKKASLCGLGQTAPNPILSTIRYFREEYEAHITDKRCPSNCCKELLLWQVIEEKCVKCGACFKACPVDAIV
WEKGQVAYLDKEKCTKCKSCYDACRFMAIE

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260656005, Length=432, Percent_Identity=37.5, Blast_Score=297, Evalue=1e-80,
Organism=Homo sapiens, GI20149568, Length=425, Percent_Identity=37.8823529411765, Blast_Score=296, Evalue=3e-80,
Organism=Escherichia coli, GI1788620, Length=402, Percent_Identity=42.0398009950249, Blast_Score=345, Evalue=4e-96,
Organism=Caenorhabditis elegans, GI17531727, Length=378, Percent_Identity=39.9470899470899, Blast_Score=292, Evalue=3e-79,
Organism=Drosophila melanogaster, GI19920794, Length=375, Percent_Identity=40.2666666666667, Blast_Score=296, Evalue=3e-80,
Organism=Drosophila melanogaster, GI45550452, Length=389, Percent_Identity=39.8457583547558, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24653819, Length=383, Percent_Identity=31.5926892950392, Blast_Score=207, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24653817, Length=355, Percent_Identity=29.8591549295775, Blast_Score=177, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 64337; Mature: 64205

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS00645 COMPLEX1_51K_2 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
5.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
8.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEAIKILICQGTGGVSAGAKKVEAEFNRIISERGVNAVVGKRCDIIKTGCRGLCANDVL
CCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHCCCCCCCCCEE
VDIVDPELGRVTYDFVQPEEVAQLVDEHIIKRTPLEKRKAKPYYNTFVDQQMRIVMTGCG
EEECCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHEEEEECCC
QIDPESLDAYLAEDGFKAIEKCVKEMTPAAVIDEVKKAGLRGRGGGGFPTGMKWSFCAAT
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCC
PGKLKYLICNADEGDPGAFMDRSILEGDPYCIIEGMMIAAYAIGCSKGYVYVRAEYPLAI
CCCEEEEEEECCCCCCCCHHCCHHHCCCCEEEEHHHHHHHHHHCCCCCEEEEEECCCHHH
ERLQKALDVCREKGYLGHNIQGWGFDFDLIIKKGAGAFVCGEETALMASIEGERGMPRPR
HHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCEEEEEECCCCCCCCCC
PPFPAVKGLWAKPTNINNVETFANVRHIINKGAEWYASLGTDTTKGTKIFAVTGKVKHTG
CCCHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEEECCCCCCC
LVEVPAGMTVRDVIYNVCGGIANNRKFKAVQAGGPSGGCIPPEVLDTPVDYDSLIKAGAM
EEECCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHCCCCCHHHHHHHHHE
MGSGGLVVMDETTCMVDVARFFLSFTRMESCGKCVPCRIGLKVMLDILERITAGRGQAGD
ECCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
IETLLEMGSTIKKASLCGLGQTAPNPILSTIRYFREEYEAHITDKRCPSNCCKELLLWQV
HHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
IEEKCVKCGACFKACPVDAIVWEKGQVAYLDKEKCTKCKSCYDACRFMAIE
HHHHHHHHHHHHHHCCCCCEEECCCCEEEECHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GEAIKILICQGTGGVSAGAKKVEAEFNRIISERGVNAVVGKRCDIIKTGCRGLCANDVL
CCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHCCCCCCCCCEE
VDIVDPELGRVTYDFVQPEEVAQLVDEHIIKRTPLEKRKAKPYYNTFVDQQMRIVMTGCG
EEECCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHEEEEECCC
QIDPESLDAYLAEDGFKAIEKCVKEMTPAAVIDEVKKAGLRGRGGGGFPTGMKWSFCAAT
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCC
PGKLKYLICNADEGDPGAFMDRSILEGDPYCIIEGMMIAAYAIGCSKGYVYVRAEYPLAI
CCCEEEEEEECCCCCCCCHHCCHHHCCCCEEEEHHHHHHHHHHCCCCCEEEEEECCCHHH
ERLQKALDVCREKGYLGHNIQGWGFDFDLIIKKGAGAFVCGEETALMASIEGERGMPRPR
HHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCEEEEEECCCCCCCCCC
PPFPAVKGLWAKPTNINNVETFANVRHIINKGAEWYASLGTDTTKGTKIFAVTGKVKHTG
CCCHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEEECCCCCCC
LVEVPAGMTVRDVIYNVCGGIANNRKFKAVQAGGPSGGCIPPEVLDTPVDYDSLIKAGAM
EEECCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHCCCCCHHHHHHHHHE
MGSGGLVVMDETTCMVDVARFFLSFTRMESCGKCVPCRIGLKVMLDILERITAGRGQAGD
ECCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
IETLLEMGSTIKKASLCGLGQTAPNPILSTIRYFREEYEAHITDKRCPSNCCKELLLWQV
HHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
IEEKCVKCGACFKACPVDAIVWEKGQVAYLDKEKCTKCKSCYDACRFMAIE
HHHHHHHHHHHHHHCCCCCEEECCCCEEEECHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]