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The map label for this gene is hydA [H]

Identifier: 78224523

GI number: 78224523

Start: 3746145

End: 3747284

Strand: Reverse

Name: hydA [H]

Synonym: Gmet_3332

Alternate gene names: 78224523

Gene position: 3747284-3746145 (Counterclockwise)

Preceding gene: 78224524

Following gene: 78224522

Centisome position: 93.74

GC content: 67.11

Gene sequence:

>1140_bases
ATGGCGAAGAATGGTGATGAGCATGACGAGATGAAGCAGCATTGTTCCGGCACGTCGGGGCCCTGGGAGGAGCGGGGGGT
ATCCCGGCGCGACTTCCTGAAGTTCTGCACCGCCATGTCGGCGGCCTTGGCCCTGCCGGTCTCCCTGGCGCCACGCATTG
CCGAGGCGCTGGAGAGCGACAGTCGGCCGTCGGTGATCTGGCTCGAATTCCAGAGTTGCACCGGCGACACCGAGGCGCTC
CTGCGGGCCGCTAACCCCACGGTGGGTGAGATCGTCCTCGACGTCCTCTCCATTGATTATGCCGAGACCATCATGGCCGC
CGCCGGCCACCAGGCCGAGGAGGCGCGGCTGAAGACCCTGAAAGAGCGAAGTGGCAAGTACATCGCCGTCGTCGAGGGGG
CGATCCCCATGAAGGACAACGGCGTCTACTGCTGCGTCGGCGGGAGATCGGCCGTGGATATCGCCCGGGAGGTCTGCGGC
GGCGCCATGGCCACCATCACCGTCGGCACCTGCGCCTCCTACGGCGGCATCCCGGCTGCATCCCCCAACCCCACCGGCGC
CGTGGGGGTCAAGGACGCGGTCCCCGGCGCCACGGTCATCAACCTTCCCGGCTGCCCCGTCAACACCGACAATCTGGTGG
CCACCGTGGTCCATATCCTCACCTTCGGCAAGCTCCCGGCCACCGACAGCAAGGGACGCCCCCTTTTCGCCTACGGCAAG
CGGATTCACGACAACTGCGAACGCCGCCCCCACTTCGACGCCGGCCAGTACGTGGAGCAATGGGGCGACCAGGCCCACCG
TGCCGGCCACTGCCTCTACAAGATGGGGTGCAAGGGGCCCGAAACCTTCCACAACTGCCCGACCCAGCGCTACAACGAGA
AGACGAGCTGGCCAGTGGGATCAGGCCACGGCTGTGCCGGCTGCTCCGAGCCCCACTTCTGGGACACCATGACCCCCTTC
TACCGGCGGCTTCCCAGCGTTCCCGGTTTCGGGATCGAGGCCACGGCCGACAAGATCGGCCTTGGCGTCGCTGCGGCCAC
GGCGGCGGTCTTCGGCATCCACGGCGTGGTGAGCGCGCTGCGCAAGGGAGATGAATCCGACGGGGAAGGAGGGGTAGACC
ATGGCCAGGATCGTCGTTGA

Upstream 100 bases:

>100_bases
AGCGATTTGACATAAGAGAATATCGTTATAAAGTCGATGTAGGCAGTCTGCCGTTCGTTGTGGTGATGTCACGCTGTATT
CTGTCCGGGGAGGAATGTCC

Downstream 100 bases:

>100_bases
CCCGATTACGAGAATAGAGGGGCACCTCCGTATCCAGGCCGAGGTGAACGGGGGCCAGATAACCGATGCCTGGAGCTCCA
GCACCATGTTCCGGGGGATC

Product: Ni-Fe hydrogenase, small subunit:twin-arginine translocation pathway signal

Products: NA

Alternate protein names: Hydrogen:quinone oxidoreductase; Membrane-bound hydrogenase small subunit [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MAKNGDEHDEMKQHCSGTSGPWEERGVSRRDFLKFCTAMSAALALPVSLAPRIAEALESDSRPSVIWLEFQSCTGDTEAL
LRAANPTVGEIVLDVLSIDYAETIMAAAGHQAEEARLKTLKERSGKYIAVVEGAIPMKDNGVYCCVGGRSAVDIAREVCG
GAMATITVGTCASYGGIPAASPNPTGAVGVKDAVPGATVINLPGCPVNTDNLVATVVHILTFGKLPATDSKGRPLFAYGK
RIHDNCERRPHFDAGQYVEQWGDQAHRAGHCLYKMGCKGPETFHNCPTQRYNEKTSWPVGSGHGCAGCSEPHFWDTMTPF
YRRLPSVPGFGIEATADKIGLGVAAATAAVFGIHGVVSALRKGDESDGEGGVDHGQDRR

Sequences:

>Translated_379_residues
MAKNGDEHDEMKQHCSGTSGPWEERGVSRRDFLKFCTAMSAALALPVSLAPRIAEALESDSRPSVIWLEFQSCTGDTEAL
LRAANPTVGEIVLDVLSIDYAETIMAAAGHQAEEARLKTLKERSGKYIAVVEGAIPMKDNGVYCCVGGRSAVDIAREVCG
GAMATITVGTCASYGGIPAASPNPTGAVGVKDAVPGATVINLPGCPVNTDNLVATVVHILTFGKLPATDSKGRPLFAYGK
RIHDNCERRPHFDAGQYVEQWGDQAHRAGHCLYKMGCKGPETFHNCPTQRYNEKTSWPVGSGHGCAGCSEPHFWDTMTPF
YRRLPSVPGFGIEATADKIGLGVAAATAAVFGIHGVVSALRKGDESDGEGGVDHGQDRR
>Mature_378_residues
AKNGDEHDEMKQHCSGTSGPWEERGVSRRDFLKFCTAMSAALALPVSLAPRIAEALESDSRPSVIWLEFQSCTGDTEALL
RAANPTVGEIVLDVLSIDYAETIMAAAGHQAEEARLKTLKERSGKYIAVVEGAIPMKDNGVYCCVGGRSAVDIAREVCGG
AMATITVGTCASYGGIPAASPNPTGAVGVKDAVPGATVINLPGCPVNTDNLVATVVHILTFGKLPATDSKGRPLFAYGKR
IHDNCERRPHFDAGQYVEQWGDQAHRAGHCLYKMGCKGPETFHNCPTQRYNEKTSWPVGSGHGCAGCSEPHFWDTMTPFY
RRLPSVPGFGIEATADKIGLGVAAATAAVFGIHGVVSALRKGDESDGEGGVDHGQDRR

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789371, Length=287, Percent_Identity=50.8710801393728, Blast_Score=311, Evalue=5e-86,
Organism=Escherichia coli, GI1787206, Length=319, Percent_Identity=44.8275862068966, Blast_Score=301, Evalue=4e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.5.1 [H]

Molecular weight: Translated: 40187; Mature: 40055

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNGDEHDEMKQHCSGTSGPWEERGVSRRDFLKFCTAMSAALALPVSLAPRIAEALESD
CCCCCCHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCC
SRPSVIWLEFQSCTGDTEALLRAANPTVGEIVLDVLSIDYAETIMAAAGHQAEEARLKTL
CCCCEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KERSGKYIAVVEGAIPMKDNGVYCCVGGRSAVDIAREVCGGAMATITVGTCASYGGIPAA
HHHCCCEEEEEECCCEECCCCEEEEECCCHHHHHHHHHHCCCEEEEEHHHHHCCCCCCCC
SPNPTGAVGVKDAVPGATVINLPGCPVNTDNLVATVVHILTFGKLPATDSKGRPLFAYGK
CCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHH
RIHDNCERRPHFDAGQYVEQWGDQAHRAGHCLYKMGCKGPETFHNCPTQRYNEKTSWPVG
HHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHCCCCHHCCCCCCCCCC
SGHGCAGCSEPHFWDTMTPFYRRLPSVPGFGIEATADKIGLGVAAATAAVFGIHGVVSAL
CCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RKGDESDGEGGVDHGQDRR
HCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKNGDEHDEMKQHCSGTSGPWEERGVSRRDFLKFCTAMSAALALPVSLAPRIAEALESD
CCCCCHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCC
SRPSVIWLEFQSCTGDTEALLRAANPTVGEIVLDVLSIDYAETIMAAAGHQAEEARLKTL
CCCCEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KERSGKYIAVVEGAIPMKDNGVYCCVGGRSAVDIAREVCGGAMATITVGTCASYGGIPAA
HHHCCCEEEEEECCCEECCCCEEEEECCCHHHHHHHHHHCCCEEEEEHHHHHCCCCCCCC
SPNPTGAVGVKDAVPGATVINLPGCPVNTDNLVATVVHILTFGKLPATDSKGRPLFAYGK
CCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHH
RIHDNCERRPHFDAGQYVEQWGDQAHRAGHCLYKMGCKGPETFHNCPTQRYNEKTSWPVG
HHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHCCCCHHCCCCCCCCCC
SGHGCAGCSEPHFWDTMTPFYRRLPSVPGFGIEATADKIGLGVAAATAAVFGIHGVVSAL
CCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RKGDESDGEGGVDHGQDRR
HCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1587288; 8319698; 14500908 [H]