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The map label for this gene is hupB [H]

Identifier: 78224522

GI number: 78224522

Start: 3744464

End: 3746164

Strand: Reverse

Name: hupB [H]

Synonym: Gmet_3331

Alternate gene names: 78224522

Gene position: 3746164-3744464 (Counterclockwise)

Preceding gene: 78224523

Following gene: 78224521

Centisome position: 93.71

GC content: 64.9

Gene sequence:

>1701_bases
ATGGCCAGGATCGTCGTTGACCCGATTACGAGAATAGAGGGGCACCTCCGTATCCAGGCCGAGGTGAACGGGGGCCAGAT
AACCGATGCCTGGAGCTCCAGCACCATGTTCCGGGGGATCGAGAAGATCCTCAAAGGGCGCGATCCCCGGGACGCCTGGT
ACTTCACCCAGCGCTTCTGCGGCGTCTGCACCACGGTCCACTCCATAGCCTCCATCCGGGCCGTGGAGAACGCCCTGAAC
ATCCGCGTCCCCCTGAACGCGGAGTTGATCAGGAACCTCATCATCGGCATCCAGGTGGTGCAGGACCACGTGATCCACTT
CTACCACCTCCACGCCCTCGACTGGGTGGACGTGGTCTCAGCTCTCAAGGCCGACCCCACAAAGACCGCAGCCTTGGCTG
CCTCCCTTTCCGACTGGCCCCTCAACTCCCCCACCTACTTCAAGTCGGTACAGGACAAGGTGGCCGCCTTCGTGAAAAAA
GGGCGCCTTGGCCCCTTCGGCAACGCCTACTGGGGACACCCGGCCTACAAGCTGCCGCCCGAGGCGAACCTCATGGCCAC
GGCCCACTACCTGGAGGCCCTGGACTGGCAACGGGAGGTCATCAAGATCCACGCCATCCTCGGCTCCAAAAACCCCCACC
CCCAGACCTTCCTCGTCGGTGGCATGGCGATCCCCGTGGATCCCAACTCCCAGAACGCCCTCAACGCCGACAAGATCGCC
GAGATTGCCCAGCTCCTCAAAAAGGCCAAGGCGTTCGTGGAGAAGGTCTACATTCCCGATCTCCTGGCGGTGGCCTCCTT
CTACAAGGAGTGGGCCGGAATCGGCGGAGGGGTGGGGAACTACATCTCCTACGGCGAATACCCGCTGGACAACAGCGGCA
AGCCGGAAAAGCTCTGGTTTCCCCAGGGGATCATCGTGGGCAAGGACCTCTCCCGGGTCTATCCCATCGACCAGCAAAAG
ATCACCGAGAGCGTGGCCCACTCCTGGTACGAGGGGGGTGATGCCGGGGTCCATCCGTGGGAAGAGCAGACCGAGGTACG
GTACACTGGCCCCACGCCCCCCTACGACATGATCGACACGGACAAGAAGTACTCCTGGGTGAAATCCCCCCGCTACGACG
GCAAGCCGATGGAGACCGGCCCCCTGGCCACCATCCTCGTGGCCTACGCCGCGGGGCACCAGGGGACGAAAGGGGCGGTG
GAGCTGGTCCTCGGCAAGCTGGGGGTCGGCCCGGAGGCGCTCTTCTCGACGCTCGGTCGGACCGCGGCCCGGGGGATCGA
GACCCTGTTGGTGGCTCAGGAACTTCCCAAGTGGCTCGACATGCTCGTGGCCAACGTGGCCGGCGGCGATCTGGCGATTC
ATAACGGCGAGAAGTGGGACCCCGCCACCTGGCCCCGGGAGACGGCGGGGTACGGCTGGCACAACGCTCCCCGGGGTGCT
CTCGGTCACTGGATCAGGATCAGGGACCAGAAAATCGAGAATTACCAGGCGGTGGTCCCCTCCACCTGGAACGCGTCACC
CCGGGATGAGAAGGGGCAGCGGGGCCCCTACGAGGCGGCCCTGGTCGGCACCCCCATGGCCGACCCCAACAAGCCCCTGG
AGATCCTCCGCACCATCCACTCCTTCGATCCGTGCCTCGCCTGCGCCGTCCACGTGGTCGATCCCCGCGGCGGCGAGCTG
GTGAGCGTCAAGGTCCTGTAG

Upstream 100 bases:

>100_bases
CTTGGCGTCGCTGCGGCCACGGCGGCGGTCTTCGGCATCCACGGCGTGGTGAGCGCGCTGCGCAAGGGAGATGAATCCGA
CGGGGAAGGAGGGGTAGACC

Downstream 100 bases:

>100_bases
GAGGTCGCCATGGCACGGAACCGGAGCTGTCTTTACGAAAAGTACGTCTGGGAGGTCCCGGTGCGGCTGACCCACTGGGT
CAACGTGATCGCCATCATCG

Product: nickel-dependent hydrogenase, large subunit

Products: NA

Alternate protein names: Hydrogenlyase; Membrane-bound hydrogenase large subunit [H]

Number of amino acids: Translated: 566; Mature: 565

Protein sequence:

>566_residues
MARIVVDPITRIEGHLRIQAEVNGGQITDAWSSSTMFRGIEKILKGRDPRDAWYFTQRFCGVCTTVHSIASIRAVENALN
IRVPLNAELIRNLIIGIQVVQDHVIHFYHLHALDWVDVVSALKADPTKTAALAASLSDWPLNSPTYFKSVQDKVAAFVKK
GRLGPFGNAYWGHPAYKLPPEANLMATAHYLEALDWQREVIKIHAILGSKNPHPQTFLVGGMAIPVDPNSQNALNADKIA
EIAQLLKKAKAFVEKVYIPDLLAVASFYKEWAGIGGGVGNYISYGEYPLDNSGKPEKLWFPQGIIVGKDLSRVYPIDQQK
ITESVAHSWYEGGDAGVHPWEEQTEVRYTGPTPPYDMIDTDKKYSWVKSPRYDGKPMETGPLATILVAYAAGHQGTKGAV
ELVLGKLGVGPEALFSTLGRTAARGIETLLVAQELPKWLDMLVANVAGGDLAIHNGEKWDPATWPRETAGYGWHNAPRGA
LGHWIRIRDQKIENYQAVVPSTWNASPRDEKGQRGPYEAALVGTPMADPNKPLEILRTIHSFDPCLACAVHVVDPRGGEL
VSVKVL

Sequences:

>Translated_566_residues
MARIVVDPITRIEGHLRIQAEVNGGQITDAWSSSTMFRGIEKILKGRDPRDAWYFTQRFCGVCTTVHSIASIRAVENALN
IRVPLNAELIRNLIIGIQVVQDHVIHFYHLHALDWVDVVSALKADPTKTAALAASLSDWPLNSPTYFKSVQDKVAAFVKK
GRLGPFGNAYWGHPAYKLPPEANLMATAHYLEALDWQREVIKIHAILGSKNPHPQTFLVGGMAIPVDPNSQNALNADKIA
EIAQLLKKAKAFVEKVYIPDLLAVASFYKEWAGIGGGVGNYISYGEYPLDNSGKPEKLWFPQGIIVGKDLSRVYPIDQQK
ITESVAHSWYEGGDAGVHPWEEQTEVRYTGPTPPYDMIDTDKKYSWVKSPRYDGKPMETGPLATILVAYAAGHQGTKGAV
ELVLGKLGVGPEALFSTLGRTAARGIETLLVAQELPKWLDMLVANVAGGDLAIHNGEKWDPATWPRETAGYGWHNAPRGA
LGHWIRIRDQKIENYQAVVPSTWNASPRDEKGQRGPYEAALVGTPMADPNKPLEILRTIHSFDPCLACAVHVVDPRGGEL
VSVKVL
>Mature_565_residues
ARIVVDPITRIEGHLRIQAEVNGGQITDAWSSSTMFRGIEKILKGRDPRDAWYFTQRFCGVCTTVHSIASIRAVENALNI
RVPLNAELIRNLIIGIQVVQDHVIHFYHLHALDWVDVVSALKADPTKTAALAASLSDWPLNSPTYFKSVQDKVAAFVKKG
RLGPFGNAYWGHPAYKLPPEANLMATAHYLEALDWQREVIKIHAILGSKNPHPQTFLVGGMAIPVDPNSQNALNADKIAE
IAQLLKKAKAFVEKVYIPDLLAVASFYKEWAGIGGGVGNYISYGEYPLDNSGKPEKLWFPQGIIVGKDLSRVYPIDQQKI
TESVAHSWYEGGDAGVHPWEEQTEVRYTGPTPPYDMIDTDKKYSWVKSPRYDGKPMETGPLATILVAYAAGHQGTKGAVE
LVLGKLGVGPEALFSTLGRTAARGIETLLVAQELPKWLDMLVANVAGGDLAIHNGEKWDPATWPRETAGYGWHNAPRGAL
GHWIRIRDQKIENYQAVVPSTWNASPRDEKGQRGPYEAALVGTPMADPNKPLEILRTIHSFDPCLACAVHVVDPRGGELV
SVKVL

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=580, Percent_Identity=49.3103448275862, Blast_Score=568, Evalue=1e-163,
Organism=Escherichia coli, GI1789368, Length=577, Percent_Identity=48.8734835355286, Blast_Score=541, Evalue=1e-155,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 62282; Mature: 62151

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARIVVDPITRIEGHLRIQAEVNGGQITDAWSSSTMFRGIEKILKGRDPRDAWYFTQRFC
CCEEEECHHHHCCCEEEEEEEECCCEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHH
GVCTTVHSIASIRAVENALNIRVPLNAELIRNLIIGIQVVQDHVIHFYHLHALDWVDVVS
HHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALKADPTKTAALAASLSDWPLNSPTYFKSVQDKVAAFVKKGRLGPFGNAYWGHPAYKLPP
HHCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
EANLMATAHYLEALDWQREVIKIHAILGSKNPHPQTFLVGGMAIPVDPNSQNALNADKIA
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCEEEECCCCCCCCCCHHHHH
EIAQLLKKAKAFVEKVYIPDLLAVASFYKEWAGIGGGVGNYISYGEYPLDNSGKPEKLWF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCEEEC
PQGIIVGKDLSRVYPIDQQKITESVAHSWYEGGDAGVHPWEEQTEVRYTGPTPPYDMIDT
CCCEEECCCHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCC
DKKYSWVKSPRYDGKPMETGPLATILVAYAAGHQGTKGAVELVLGKLGVGPEALFSTLGR
CCCHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHH
TAARGIETLLVAQELPKWLDMLVANVAGGDLAIHNGEKWDPATWPRETAGYGWHNAPRGA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCH
LGHWIRIRDQKIENYQAVVPSTWNASPRDEKGQRGPYEAALVGTPMADPNKPLEILRTIH
HHCEEEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHH
SFDPCLACAVHVVDPRGGELVSVKVL
CCCHHHHHEEEEECCCCCCEEEEEEC
>Mature Secondary Structure 
ARIVVDPITRIEGHLRIQAEVNGGQITDAWSSSTMFRGIEKILKGRDPRDAWYFTQRFC
CEEEECHHHHCCCEEEEEEEECCCEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHH
GVCTTVHSIASIRAVENALNIRVPLNAELIRNLIIGIQVVQDHVIHFYHLHALDWVDVVS
HHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALKADPTKTAALAASLSDWPLNSPTYFKSVQDKVAAFVKKGRLGPFGNAYWGHPAYKLPP
HHCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
EANLMATAHYLEALDWQREVIKIHAILGSKNPHPQTFLVGGMAIPVDPNSQNALNADKIA
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCEEEECCCCCCCCCCHHHHH
EIAQLLKKAKAFVEKVYIPDLLAVASFYKEWAGIGGGVGNYISYGEYPLDNSGKPEKLWF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCEEEC
PQGIIVGKDLSRVYPIDQQKITESVAHSWYEGGDAGVHPWEEQTEVRYTGPTPPYDMIDT
CCCEEECCCHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCC
DKKYSWVKSPRYDGKPMETGPLATILVAYAAGHQGTKGAVELVLGKLGVGPEALFSTLGR
CCCHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHH
TAARGIETLLVAQELPKWLDMLVANVAGGDLAIHNGEKWDPATWPRETAGYGWHNAPRGA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCH
LGHWIRIRDQKIENYQAVVPSTWNASPRDEKGQRGPYEAALVGTPMADPNKPLEILRTIH
HHCEEEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHH
SFDPCLACAVHVVDPRGGELVSVKVL
CCCHHHHHEEEEECCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3054886; 12597275; 8163174 [H]