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The map label for this gene is cheB5

Identifier: 78224460

GI number: 78224460

Start: 3678338

End: 3679411

Strand: Direct

Name: cheB5

Synonym: Gmet_3269

Alternate gene names: 78224460

Gene position: 3678338-3679411 (Clockwise)

Preceding gene: 78224459

Following gene: 78224462

Centisome position: 92.02

GC content: 64.06

Gene sequence:

>1074_bases
ATGCTTCCGAATCAGGAAAGAAAACTGCGCGTACTCGTTGTGGACGACTCGTCCTTCATGCGGATGGTCATCCGGAGCGT
GCTGGAGAAGGATCCCGCCATCGAGGTCATCGCCGTGGCCATGGACGGGGTCGAGGGGGTGGAAAAGGCCCTGGCCCTCA
GACCGGACCTCATAACCATGGACATCGAGATGCCCCGGCTCGACGGCATCTCAGCCCTCAAGGAGATCATGGCCAAGGCC
CCGACCAGGGTTCTCATGGTCTCCACCCTCACCTGCGAGGGTGCCAAGGCCACCTTCGACGCCCTCGACGCCGGCGCCAT
CGACTACATCCCCAAGAACGTCACCGACAGCATTGACGCCCAGAAAGCCTTCAAGGAGGAGCTCCTGCGGAAGGTGAAGG
GGTCCGGCATATCGATCCTCGGCAGGCCCCTGGTCTCCCCTTCCCCTCGCCTCGTGGTGCCGCCGCGCCCTGTGATAATC
CCCCGACCGGCGGGGCAGCGGTACCAGTACGTGGGAATCGGCGCCTCCACCGGCGGGCCGGTGGCCGTGCAGGAGGTTCT
CGGACGGATCCCGGGCAACTTCCCCCACGGGATCGTGGTGGCCATCCACATGCCCAAGGCGTTTACCGGCCCTTACGCAG
AGCGGCTCAACACCAAGTGCTCCCTCCAGGTAAAGGAGGCGAAGGCCGGCGACATCGTCCAACCGGGCGTTGTGCTCGTG
ACCCCAGGCGGGATGCACACGGCGCTGGTCCGCCAGGGAAGCACCGTCGCCATCCGGACCATTGCCACGGCCGAGTATCC
CCAGTACATCTACATCCCGTCGGTGGACCACATGCTGACCACCTTTGCCGACGCCTGCAACGGTTCACTCCTCGGGGTCA
TCCTTACGGGGATGGGAGCTGACGGTTTCAAGGGAATGAAGCACCTAAAGACCAAGGGGGGAGGAACTATCGTTCAGGAT
GAGGCCACCTCCACCATCTACGGCATGCCCCGGGCCTGCATCGAGGGGGGGGTGGCCGATACGGTCCTGCCGCTCACCCA
GATCGGCACCGAGATCACGAAGATCGCAGGTTAG

Upstream 100 bases:

>100_bases
TGCGGAGTTTGACGTGGAACGGTTTTGCTACGAACTGGACGACCAGGTGGAGGAGATCCGGGAACTGGTCACGAACATCT
TCAAGGACAGGTGACAAAGC

Downstream 100 bases:

>100_bases
CGCCGGGCAGTAAGGGCAAGGCCCTCTGGAAGGAGACGAACGCGGCACCGGGAGAATCCGAGCCGTTTGAGGCGAAAGAG
GAGAAAAGGGGCAGCCAGGG

Product: response regulator receiver (CheY-like) modulated CheB methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MLPNQERKLRVLVVDDSSFMRMVIRSVLEKDPAIEVIAVAMDGVEGVEKALALRPDLITMDIEMPRLDGISALKEIMAKA
PTRVLMVSTLTCEGAKATFDALDAGAIDYIPKNVTDSIDAQKAFKEELLRKVKGSGISILGRPLVSPSPRLVVPPRPVII
PRPAGQRYQYVGIGASTGGPVAVQEVLGRIPGNFPHGIVVAIHMPKAFTGPYAERLNTKCSLQVKEAKAGDIVQPGVVLV
TPGGMHTALVRQGSTVAIRTIATAEYPQYIYIPSVDHMLTTFADACNGSLLGVILTGMGADGFKGMKHLKTKGGGTIVQD
EATSTIYGMPRACIEGGVADTVLPLTQIGTEITKIAG

Sequences:

>Translated_357_residues
MLPNQERKLRVLVVDDSSFMRMVIRSVLEKDPAIEVIAVAMDGVEGVEKALALRPDLITMDIEMPRLDGISALKEIMAKA
PTRVLMVSTLTCEGAKATFDALDAGAIDYIPKNVTDSIDAQKAFKEELLRKVKGSGISILGRPLVSPSPRLVVPPRPVII
PRPAGQRYQYVGIGASTGGPVAVQEVLGRIPGNFPHGIVVAIHMPKAFTGPYAERLNTKCSLQVKEAKAGDIVQPGVVLV
TPGGMHTALVRQGSTVAIRTIATAEYPQYIYIPSVDHMLTTFADACNGSLLGVILTGMGADGFKGMKHLKTKGGGTIVQD
EATSTIYGMPRACIEGGVADTVLPLTQIGTEITKIAG
>Mature_357_residues
MLPNQERKLRVLVVDDSSFMRMVIRSVLEKDPAIEVIAVAMDGVEGVEKALALRPDLITMDIEMPRLDGISALKEIMAKA
PTRVLMVSTLTCEGAKATFDALDAGAIDYIPKNVTDSIDAQKAFKEELLRKVKGSGISILGRPLVSPSPRLVVPPRPVII
PRPAGQRYQYVGIGASTGGPVAVQEVLGRIPGNFPHGIVVAIHMPKAFTGPYAERLNTKCSLQVKEAKAGDIVQPGVVLV
TPGGMHTALVRQGSTVAIRTIATAEYPQYIYIPSVDHMLTTFADACNGSLLGVILTGMGADGFKGMKHLKTKGGGTIVQD
EATSTIYGMPRACIEGGVADTVLPLTQIGTEITKIAG

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=347, Percent_Identity=34.2939481268012, Blast_Score=200, Evalue=1e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB5_GEOMG (Q39QJ2)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_386207.1
- ProteinModelPortal:   Q39QJ2
- SMR:   Q39QJ2
- STRING:   Q39QJ2
- GeneID:   3741253
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_3269
- NMPDR:   fig|269799.3.peg.2504
- eggNOG:   COG2201
- HOGENOM:   HBG705324
- OMA:   KMAAELN
- PhylomeDB:   Q39QJ2
- ProtClustDB:   CLSK827727
- BioCyc:   GMET269799:GMET_3269-MONOMER
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 37876; Mature: 37876

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 176-176 ACT_SITE 203-203 ACT_SITE 301-301

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPNQERKLRVLVVDDSSFMRMVIRSVLEKDPAIEVIAVAMDGVEGVEKALALRPDLITM
CCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHCCCCEEEE
DIEMPRLDGISALKEIMAKAPTRVLMVSTLTCEGAKATFDALDAGAIDYIPKNVTDSIDA
EECCCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHCCCCHHCCCCCCCCCHHH
QKAFKEELLRKVKGSGISILGRPLVSPSPRLVVPPRPVIIPRPAGQRYQYVGIGASTGGP
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEECCCCEEEECCCCCCEEEEEEECCCCCC
VAVQEVLGRIPGNFPHGIVVAIHMPKAFTGPYAERLNTKCSLQVKEAKAGDIVQPGVVLV
HHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHCCCEEEEEEECCCCCCCCCCCEEEE
TPGGMHTALVRQGSTVAIRTIATAEYPQYIYIPSVDHMLTTFADACNGSLLGVILTGMGA
CCCCCEEHHHCCCCEEEEEEEEECCCCCEEEECCHHHHHHHHHHHCCCCEEEEEEECCCC
DGFKGMKHLKTKGGGTIVQDEATSTIYGMPRACIEGGVADTVLPLTQIGTEITKIAG
CHHHHHHHHHCCCCCEEEECCCCCHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLPNQERKLRVLVVDDSSFMRMVIRSVLEKDPAIEVIAVAMDGVEGVEKALALRPDLITM
CCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHCCCCEEEE
DIEMPRLDGISALKEIMAKAPTRVLMVSTLTCEGAKATFDALDAGAIDYIPKNVTDSIDA
EECCCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHCCCCHHCCCCCCCCCHHH
QKAFKEELLRKVKGSGISILGRPLVSPSPRLVVPPRPVIIPRPAGQRYQYVGIGASTGGP
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEECCCCEEEECCCCCCEEEEEEECCCCCC
VAVQEVLGRIPGNFPHGIVVAIHMPKAFTGPYAERLNTKCSLQVKEAKAGDIVQPGVVLV
HHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHCCCEEEEEEECCCCCCCCCCCEEEE
TPGGMHTALVRQGSTVAIRTIATAEYPQYIYIPSVDHMLTTFADACNGSLLGVILTGMGA
CCCCCEEHHHCCCCEEEEEEEEECCCCCEEEECCHHHHHHHHHHHCCCCEEEEEEECCCC
DGFKGMKHLKTKGGGTIVQDEATSTIYGMPRACIEGGVADTVLPLTQIGTEITKIAG
CHHHHHHHHHCCCCCEEEECCCCCHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA