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The map label for this gene is glcF [H]

Identifier: 78224437

GI number: 78224437

Start: 3652167

End: 3653432

Strand: Direct

Name: glcF [H]

Synonym: Gmet_3246

Alternate gene names: 78224437

Gene position: 3652167-3653432 (Clockwise)

Preceding gene: 78224436

Following gene: 78224438

Centisome position: 91.36

GC content: 66.35

Gene sequence:

>1266_bases
GTGGATCCTCTCAAACGAGTCGAAAACGAACTGAAAAAATGCGTGAAGTGCGGCGCCTGCCGGGCCCACTGCCCCGCTTT
CGCCACCTTCGGCAAGGAGCCGGCCGTGGCCCGGGGGAAGGTGGCCCTGGCCCAGCACCTCCTCAAGGGGGACATCGAGC
TGGACGACGCCACCTACGCCGCCATGTCCAAGTGCCTCCTCTGCGGAAGCTGCGTGGAAAAGTGTCCCAACGACGTCCCC
ACGGACGAGATCGTCGTGGTGGCCCGGGAGGCCCTCTCGGGCCAGCGGGGACTCACCACCTTCCACGCGGCGGTGGGGCG
GGTGATCAGGAATCGCTCTCTCATGAAATTTGGCGCCGCGGCGGCCCGGATTCTCGGTCCCCTCTTTTTCCGCAAGGTGC
CGGAGACCTCGGGGCTGCGGCTCCGCTTCCCGCTCCCCTTTTTGGGGGGGAAGCGCCACATCCCCTCCATCGCGAAAAAG
CCGTTCCTGGACCGCCACCCCGAAGTGATTCCAGGGGAACCGGGAAAGCCGCGCATCGTCTACTTCGTCGGCTGCATGAC
CAACTTCTGCTACCCCCAGGTGGGGGAGGCGGCCCTGGCCCTGTTCCGGCACCTGGGGTGCACGGTTATCATCCCCAAGA
ACCAGCAGTGCTGCGGCCTGCCGGGAATGTCCGGCGGCGACCTGGACACGGTGCGCGGGTTGGCCGAGAAGAACCTGGCC
GAGTTGGAGCGGTACGAGGCCGACTACATCATGACCGCCTGCGCCACCTGCGGCGGCGCCCTCCACAAGTTCTACCCCAA
CCTGGTAGGTAAGCGGCACCCGGAACTGGCGGCCCGCCTCAAGGCCATCGCCGAGAAGACCGTGGACGCGGCCCAGCTCC
TGCAAAAACTGGGGCTCAATCCCGAGGAAACCGGCGCCGGCAGCGACATCCGCGTCACCTACCACGACCCGTGCCACCTG
AGAACCCGCGCCATCACCCGTCAGCCCCGGGAACTGCTGCAGGGGACCCCGGGCGTGGAACTGGCCGAGATGGAGGGCGC
CGACAAGTGTTGCGGCCTCGGCGGCACCTTCAACGTCTACCACTACGAGAGTTCCCTGGCCATCAACAAGATGAAGAGCG
CCGCCATCATCAACACTGGCGCCGATGCGGTGGTCACCGGCTGCCCCGGCTGCATGATGCAACTCTCCGACGGGCTCAAG
CAGCAACGCAACCGGACCCGCGTCCTGCATACCCTCGAGATCCTCGCCCGTTCCATCCGCCCGTAA

Upstream 100 bases:

>100_bases
ATTCGACGATCAGCTGGACTTCTCAAAACACTTCTTCTTTTTAGTTACGAGCTTTTCTCCGTGCCCTCCGTCTCTCCGTG
TTATTCAAAAAGGTTTTCCC

Downstream 100 bases:

>100_bases
TCCGGCGAGTCGGCGGGACGGGGTTTCCATCCCTCTCCCGCCGACTCGTTTTTCCCCTCTGGAAAAATCGTTCTATAGTC
TCCTCAATGACGGTATCCAT

Product: hypothetical protein

Products: FADH2; dihydroxy-acetone-phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MDPLKRVENELKKCVKCGACRAHCPAFATFGKEPAVARGKVALAQHLLKGDIELDDATYAAMSKCLLCGSCVEKCPNDVP
TDEIVVVAREALSGQRGLTTFHAAVGRVIRNRSLMKFGAAAARILGPLFFRKVPETSGLRLRFPLPFLGGKRHIPSIAKK
PFLDRHPEVIPGEPGKPRIVYFVGCMTNFCYPQVGEAALALFRHLGCTVIIPKNQQCCGLPGMSGGDLDTVRGLAEKNLA
ELERYEADYIMTACATCGGALHKFYPNLVGKRHPELAARLKAIAEKTVDAAQLLQKLGLNPEETGAGSDIRVTYHDPCHL
RTRAITRQPRELLQGTPGVELAEMEGADKCCGLGGTFNVYHYESSLAINKMKSAAIINTGADAVVTGCPGCMMQLSDGLK
QQRNRTRVLHTLEILARSIRP

Sequences:

>Translated_421_residues
MDPLKRVENELKKCVKCGACRAHCPAFATFGKEPAVARGKVALAQHLLKGDIELDDATYAAMSKCLLCGSCVEKCPNDVP
TDEIVVVAREALSGQRGLTTFHAAVGRVIRNRSLMKFGAAAARILGPLFFRKVPETSGLRLRFPLPFLGGKRHIPSIAKK
PFLDRHPEVIPGEPGKPRIVYFVGCMTNFCYPQVGEAALALFRHLGCTVIIPKNQQCCGLPGMSGGDLDTVRGLAEKNLA
ELERYEADYIMTACATCGGALHKFYPNLVGKRHPELAARLKAIAEKTVDAAQLLQKLGLNPEETGAGSDIRVTYHDPCHL
RTRAITRQPRELLQGTPGVELAEMEGADKCCGLGGTFNVYHYESSLAINKMKSAAIINTGADAVVTGCPGCMMQLSDGLK
QQRNRTRVLHTLEILARSIRP
>Mature_421_residues
MDPLKRVENELKKCVKCGACRAHCPAFATFGKEPAVARGKVALAQHLLKGDIELDDATYAAMSKCLLCGSCVEKCPNDVP
TDEIVVVAREALSGQRGLTTFHAAVGRVIRNRSLMKFGAAAARILGPLFFRKVPETSGLRLRFPLPFLGGKRHIPSIAKK
PFLDRHPEVIPGEPGKPRIVYFVGCMTNFCYPQVGEAALALFRHLGCTVIIPKNQQCCGLPGMSGGDLDTVRGLAEKNLA
ELERYEADYIMTACATCGGALHKFYPNLVGKRHPELAARLKAIAEKTVDAAQLLQKLGLNPEETGAGSDIRVTYHDPCHL
RTRAITRQPRELLQGTPGVELAEMEGADKCCGLGGTFNVYHYESSLAINKMKSAAIINTGADAVVTGCPGCMMQLSDGLK
QQRNRTRVLHTLEILARSIRP

Specific function: Electron Transfer Protein; May Also Function As The Membrane Anchor For The Glpab Dimer. [C]

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Loosely Bound To The Cytoplasm Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1788576, Length=425, Percent_Identity=24.4705882352941, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1786497, Length=240, Percent_Identity=29.1666666666667, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI48994913, Length=417, Percent_Identity=24.4604316546763, Blast_Score=95, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 45687; Mature: 45687

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
4.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPLKRVENELKKCVKCGACRAHCPAFATFGKEPAVARGKVALAQHLLKGDIELDDATYA
CCHHHHHHHHHHHHHHHCCCHHCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
AMSKCLLCGSCVEKCPNDVPTDEIVVVAREALSGQRGLTTFHAAVGRVIRNRSLMKFGAA
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHH
AARILGPLFFRKVPETSGLRLRFPLPFLGGKRHIPSIAKKPFLDRHPEVIPGEPGKPRIV
HHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCEEE
YFVGCMTNFCYPQVGEAALALFRHLGCTVIIPKNQQCCGLPGMSGGDLDTVRGLAEKNLA
EEEEHHHHCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
ELERYEADYIMTACATCGGALHKFYPNLVGKRHPELAARLKAIAEKTVDAAQLLQKLGLN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PEETGAGSDIRVTYHDPCHLRTRAITRQPRELLQGTPGVELAEMEGADKCCGLGGTFNVY
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEHHCCCCHHHCCCCCCEEEE
HYESSLAINKMKSAAIINTGADAVVTGCPGCMMQLSDGLKQQRNRTRVLHTLEILARSIR
EECCHHHHHHHHHHHEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
P
C
>Mature Secondary Structure
MDPLKRVENELKKCVKCGACRAHCPAFATFGKEPAVARGKVALAQHLLKGDIELDDATYA
CCHHHHHHHHHHHHHHHCCCHHCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
AMSKCLLCGSCVEKCPNDVPTDEIVVVAREALSGQRGLTTFHAAVGRVIRNRSLMKFGAA
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHH
AARILGPLFFRKVPETSGLRLRFPLPFLGGKRHIPSIAKKPFLDRHPEVIPGEPGKPRIV
HHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCEEE
YFVGCMTNFCYPQVGEAALALFRHLGCTVIIPKNQQCCGLPGMSGGDLDTVRGLAEKNLA
EEEEHHHHCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
ELERYEADYIMTACATCGGALHKFYPNLVGKRHPELAARLKAIAEKTVDAAQLLQKLGLN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PEETGAGSDIRVTYHDPCHLRTRAITRQPRELLQGTPGVELAEMEGADKCCGLGGTFNVY
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEHHCCCCHHHCCCCCCEEEE
HYESSLAINKMKSAAIINTGADAVVTGCPGCMMQLSDGLKQQRNRTRVLHTLEILARSIR
EECCHHHHHHHHHHHEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
P
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: FAD; glycerol-3-phosphate [C]

Specific reaction: FAD + glycerol-3-phosphate = FADH2 + dihydroxy-acetone-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]