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The map label for this gene is hemC

Identifier: 78224425

GI number: 78224425

Start: 3639530

End: 3640486

Strand: Direct

Name: hemC

Synonym: Gmet_3234

Alternate gene names: 78224425

Gene position: 3639530-3640486 (Clockwise)

Preceding gene: 78224424

Following gene: 78224426

Centisome position: 91.05

GC content: 66.77

Gene sequence:

>957_bases
ATGGCACCGAAGCAGCTGAGAATAGGAACCCGGGCGAGCCAGCTTGCCCTCTGGCAGGCCAACTGGGTGAAGGGTGAGCT
GGAAAAGCGCTACCCCGGCATGGCCGTGGAACTCGTCAAGATCAAGACCATGGGGGACAAGATCCTCGACGTCCCCCTGG
CCCAGGTGGGGGGCAAGGGGCTCTTTGTCAAGGAGATTGAAGAGGCGATGCTCCGGGGCGAGATCGATATCGCGGTCCAC
AGCATGAAGGACGTCCCCACCGAATTCCCCGAGGGGCTCGGCCTCTACTGCATCACCGAGCGGGAGGACCCGCGCGATGC
CGTCATCTCCCGGGGGGTGAAATTCGCCGACCTTCCCCAGGGGGCTCGTATCGGCACCTCGGCCCTGCGCCGCCAGGCCC
AGATCCTCAAGGTGCGCCCCGACCTCCAGATGGTGGTCATCCGCGGCAACGTGGAGACCCGCATCCGCAAGCTCACCGAC
GAGAACCTGGATGCGGTCATTCTGGCCGCTGCCGGCCTGAACCGCCTCGGCTTCGCCGACCAGGTCTCCGAGTACCTGCC
GGTGGAGCTGTCGCTCCCGGCCATCGGCCAGGGGGCCCTCGGCATCGAGTGCCGCCTCGATGACGAAACCATCAAGGATA
CCATCGCCTTCTTCAACCACCCCGACACCGCCCACGCGGTCCGGGCCGAGCGGGCGCTTCTGTGGCGCTGCGAAGGCGGC
TGCCAGGTCCCCATAGCCGCCCACGGCCAGGTTTCCGGCGACACCCTCACCCTCACCGGCTTCGTCGCCTCGGTGGATGG
CACCCGCTCGGTGAAGGACACCATCTCCGGACCGGTGACGGAGTGCGAGAAGCTCGGCATTGCCCTGGCGGAGAAGCTCC
TGGCCGACGGCGCCCACGAGATCCTCGCTGAGGTCTACCAGCGGGAAGTGGCGCGGGAGAAGGAAATCCCGGTCTAG

Upstream 100 bases:

>100_bases
TCCAGACCGGCGAGTCTGAGGCGGAGGAACTTGGAGAGCTGGAAGAGTAAGACCCCTCAAAGCATTCCGCTGTTTTCATA
GATAAAAAAAGGAGACTGCA

Downstream 100 bases:

>100_bases
TCGCACCCCATGCCCGATAAATCCCTCAAAAAACCGTTCGTCCACCTCATCGGTGCCGGCCCCGGCGACCCGGGGCTCAT
CACCGTCAAGGGTCGGGAGT

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MAPKQLRIGTRASQLALWQANWVKGELEKRYPGMAVELVKIKTMGDKILDVPLAQVGGKGLFVKEIEEAMLRGEIDIAVH
SMKDVPTEFPEGLGLYCITEREDPRDAVISRGVKFADLPQGARIGTSALRRQAQILKVRPDLQMVVIRGNVETRIRKLTD
ENLDAVILAAAGLNRLGFADQVSEYLPVELSLPAIGQGALGIECRLDDETIKDTIAFFNHPDTAHAVRAERALLWRCEGG
CQVPIAAHGQVSGDTLTLTGFVASVDGTRSVKDTISGPVTECEKLGIALAEKLLADGAHEILAEVYQREVAREKEIPV

Sequences:

>Translated_318_residues
MAPKQLRIGTRASQLALWQANWVKGELEKRYPGMAVELVKIKTMGDKILDVPLAQVGGKGLFVKEIEEAMLRGEIDIAVH
SMKDVPTEFPEGLGLYCITEREDPRDAVISRGVKFADLPQGARIGTSALRRQAQILKVRPDLQMVVIRGNVETRIRKLTD
ENLDAVILAAAGLNRLGFADQVSEYLPVELSLPAIGQGALGIECRLDDETIKDTIAFFNHPDTAHAVRAERALLWRCEGG
CQVPIAAHGQVSGDTLTLTGFVASVDGTRSVKDTISGPVTECEKLGIALAEKLLADGAHEILAEVYQREVAREKEIPV
>Mature_317_residues
APKQLRIGTRASQLALWQANWVKGELEKRYPGMAVELVKIKTMGDKILDVPLAQVGGKGLFVKEIEEAMLRGEIDIAVHS
MKDVPTEFPEGLGLYCITEREDPRDAVISRGVKFADLPQGARIGTSALRRQAQILKVRPDLQMVVIRGNVETRIRKLTDE
NLDAVILAAAGLNRLGFADQVSEYLPVELSLPAIGQGALGIECRLDDETIKDTIAFFNHPDTAHAVRAERALLWRCEGGC
QVPIAAHGQVSGDTLTLTGFVASVDGTRSVKDTISGPVTECEKLGIALAEKLLADGAHEILAEVYQREVAREKEIPV

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI20149500, Length=337, Percent_Identity=40.0593471810089, Blast_Score=238, Evalue=5e-63,
Organism=Homo sapiens, GI66933009, Length=331, Percent_Identity=40.1812688821752, Blast_Score=238, Evalue=6e-63,
Organism=Escherichia coli, GI48994974, Length=308, Percent_Identity=54.2207792207792, Blast_Score=320, Evalue=6e-89,
Organism=Saccharomyces cerevisiae, GI6319996, Length=321, Percent_Identity=37.0716510903427, Blast_Score=202, Evalue=6e-53,
Organism=Drosophila melanogaster, GI20130425, Length=294, Percent_Identity=39.4557823129252, Blast_Score=207, Evalue=7e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_GEOMG (Q39QM7)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_386172.1
- HSSP:   P06983
- ProteinModelPortal:   Q39QM7
- SMR:   Q39QM7
- STRING:   Q39QM7
- GeneID:   3740950
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_3234
- NMPDR:   fig|269799.3.peg.2469
- eggNOG:   COG0181
- HOGENOM:   HBG624842
- OMA:   RATEVFD
- PhylomeDB:   Q39QM7
- ProtClustDB:   PRK00072
- BioCyc:   GMET269799:GMET_3234-MONOMER
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 34648; Mature: 34517

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPKQLRIGTRASQLALWQANWVKGELEKRYPGMAVELVKIKTMGDKILDVPLAQVGGKG
CCCCCCCCCCCHHHEEEEECCCCHHHHHHCCCCCEEEEEEEEECCCHHHCCCHHHHCCCC
LFVKEIEEAMLRGEIDIAVHSMKDVPTEFPEGLGLYCITEREDPRDAVISRGVKFADLPQ
EEHHHHHHHHHCCCCEEEEECHHCCCCCCCCCCCEEEEECCCCCHHHHHHCCCEEECCCC
GARIGTSALRRQAQILKVRPDLQMVVIRGNVETRIRKLTDENLDAVILAAAGLNRLGFAD
CCCCCHHHHHHHHHHEEECCCEEEEEEECCHHHHHHHHCCCCCCEEEEEECCCCCCCCHH
QVSEYLPVELSLPAIGQGALGIECRLDDETIKDTIAFFNHPDTAHAVRAERALLWRCEGG
HHHHHCCEEEECCCCCCCCEEEEEEECHHHHHHHHHHCCCCCHHHHHHHHHEEEEEECCC
CQVPIAAHGQVSGDTLTLTGFVASVDGTRSVKDTISGPVTECEKLGIALAEKLLADGAHE
CEEEEEECCCCCCCEEEEEEEEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHH
ILAEVYQREVAREKEIPV
HHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
APKQLRIGTRASQLALWQANWVKGELEKRYPGMAVELVKIKTMGDKILDVPLAQVGGKG
CCCCCCCCCCHHHEEEEECCCCHHHHHHCCCCCEEEEEEEEECCCHHHCCCHHHHCCCC
LFVKEIEEAMLRGEIDIAVHSMKDVPTEFPEGLGLYCITEREDPRDAVISRGVKFADLPQ
EEHHHHHHHHHCCCCEEEEECHHCCCCCCCCCCCEEEEECCCCCHHHHHHCCCEEECCCC
GARIGTSALRRQAQILKVRPDLQMVVIRGNVETRIRKLTDENLDAVILAAAGLNRLGFAD
CCCCCHHHHHHHHHHEEECCCEEEEEEECCHHHHHHHHCCCCCCEEEEEECCCCCCCCHH
QVSEYLPVELSLPAIGQGALGIECRLDDETIKDTIAFFNHPDTAHAVRAERALLWRCEGG
HHHHHCCEEEECCCCCCCCEEEEEEECHHHHHHHHHHCCCCCHHHHHHHHHEEEEEECCC
CQVPIAAHGQVSGDTLTLTGFVASVDGTRSVKDTISGPVTECEKLGIALAEKLLADGAHE
CEEEEEECCCCCCCEEEEEEEEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHH
ILAEVYQREVAREKEIPV
HHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA