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The map label for this gene is hemA

Identifier: 78224424

GI number: 78224424

Start: 3638175

End: 3639479

Strand: Direct

Name: hemA

Synonym: Gmet_3233

Alternate gene names: 78224424

Gene position: 3638175-3639479 (Clockwise)

Preceding gene: 78224423

Following gene: 78224425

Centisome position: 91.01

GC content: 61.84

Gene sequence:

>1305_bases
ATGAACATTGTGGTTGTGGGGCTGTCACACAAGACCGCCTCCGTCGATATACGGGAAAAAGTCGCCTTCGCACCGACACA
GATGGAAAAGCCGCTGCGGGCGCTATTGGCCATCGAGGACATTGCCGAAGCGGTCATCGTTTCCACCTGCAATCGGGTGG
AAGTATACATCTCCACCCGCGACATCGCCGGCGGGATGGCGCGGATCAAGCGTTTTCTGGCCGACTACCACGGCATCTCC
CCCGAAATCCTCGAACCTCACCTTTACGCCCACCACGGCGAGGCAGCCATCCGGCACGTCTTCCGGGTCGCTTCAAGCCT
CGACTCCATGGTGGTGGGAGAGCCCCAGATCCTGGGCCAGATCAAGACCGCCTACGGTTACGCCGCCGAGTTCAAGACCA
GCGGCATCATCCTCAACCGCTTCCTCCACAAGGCTTTCTCCGTGGCCAAGCGGGTGCGGACCGAAACGAAGATCGCCTCG
TCGGCCGTCTCCGTCTCCTTTGCCGCGGTGGAACTGGCCCGAAAGATTTTCGGCAATCTCTCGGACAAGACGGTCATGCT
CATCGGCGCCGGCGAGATGTGCGAACTGGCAGCCAAGCACTTCATCAACAACGGGGTGCGGGGGGTCATGGTCACCAACC
GGACCTACGAACGGGCCGTGAAGCTGGCCGAGGAGTTCGAGGGGAAACCGGTCTCCTTCGACGACCTCTTCGACCAGCTC
CATAAGGCCGACATCGTTCTCTCCTCCACCGGCGCCACCCAATTCATCATCAAACCCAAGGACGTGGAGGAGGTGATCCG
GCGCCGGAAGCTGAAGCCCATGTTCTTCATCGACATCGCGGTGCCTCGGGACATCGATCCCAAGGTGAACGACGTGGAGA
ACGTCTACCTCTATGACATGGACGACCTCCAGGGGGTCGTCGCCTCCAACCTGCAGCAGCGGGCCGAGGAGGCGAAAAAG
GCCGAGGCGATTATCGACGAGGAAATCGGCCAGTTCCACAAGTGGCTCTCCAACCTGGAGGTGACGCCGACCATCGTGGC
GCTCCGGTCGAAATTCGAGGAGACGCGGAAGGCCGAGTTGGAAAAGACCCTCGCCTCCTGGAAGGACCTCCCCCCCGACG
GGGCCAAGCGGCTGGAGGCCCTCACCACCGCCATCGTCAACAAGCTCCTCCACCCCCCTACCGCAACCCTCAAGCGGGTG
GGCCAGGGAGGGCGGACCGACCTCTACGTGGATGCCCTCCGTACCCTCTTCGAGCTCCAGACCGGCGAGTCTGAGGCGGA
GGAACTTGGAGAGCTGGAAGAGTAA

Upstream 100 bases:

>100_bases
CTCGCCATTGTCGGCTTCTTCGTCCTCCTGTTCACCTTCCTCGGCGTGAACCTGTTCCTGTCGGGGCTGCACAGTTACAA
ATAAGAAGTCAGAGGATCCC

Downstream 100 bases:

>100_bases
GACCCCTCAAAGCATTCCGCTGTTTTCATAGATAAAAAAAGGAGACTGCAATGGCACCGAAGCAGCTGAGAATAGGAACC
CGGGCGAGCCAGCTTGCCCT

Product: glutamyl-tRNA reductase

Products: NA

Alternate protein names: GluTR

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MNIVVVGLSHKTASVDIREKVAFAPTQMEKPLRALLAIEDIAEAVIVSTCNRVEVYISTRDIAGGMARIKRFLADYHGIS
PEILEPHLYAHHGEAAIRHVFRVASSLDSMVVGEPQILGQIKTAYGYAAEFKTSGIILNRFLHKAFSVAKRVRTETKIAS
SAVSVSFAAVELARKIFGNLSDKTVMLIGAGEMCELAAKHFINNGVRGVMVTNRTYERAVKLAEEFEGKPVSFDDLFDQL
HKADIVLSSTGATQFIIKPKDVEEVIRRRKLKPMFFIDIAVPRDIDPKVNDVENVYLYDMDDLQGVVASNLQQRAEEAKK
AEAIIDEEIGQFHKWLSNLEVTPTIVALRSKFEETRKAELEKTLASWKDLPPDGAKRLEALTTAIVNKLLHPPTATLKRV
GQGGRTDLYVDALRTLFELQTGESEAEELGELEE

Sequences:

>Translated_434_residues
MNIVVVGLSHKTASVDIREKVAFAPTQMEKPLRALLAIEDIAEAVIVSTCNRVEVYISTRDIAGGMARIKRFLADYHGIS
PEILEPHLYAHHGEAAIRHVFRVASSLDSMVVGEPQILGQIKTAYGYAAEFKTSGIILNRFLHKAFSVAKRVRTETKIAS
SAVSVSFAAVELARKIFGNLSDKTVMLIGAGEMCELAAKHFINNGVRGVMVTNRTYERAVKLAEEFEGKPVSFDDLFDQL
HKADIVLSSTGATQFIIKPKDVEEVIRRRKLKPMFFIDIAVPRDIDPKVNDVENVYLYDMDDLQGVVASNLQQRAEEAKK
AEAIIDEEIGQFHKWLSNLEVTPTIVALRSKFEETRKAELEKTLASWKDLPPDGAKRLEALTTAIVNKLLHPPTATLKRV
GQGGRTDLYVDALRTLFELQTGESEAEELGELEE
>Mature_434_residues
MNIVVVGLSHKTASVDIREKVAFAPTQMEKPLRALLAIEDIAEAVIVSTCNRVEVYISTRDIAGGMARIKRFLADYHGIS
PEILEPHLYAHHGEAAIRHVFRVASSLDSMVVGEPQILGQIKTAYGYAAEFKTSGIILNRFLHKAFSVAKRVRTETKIAS
SAVSVSFAAVELARKIFGNLSDKTVMLIGAGEMCELAAKHFINNGVRGVMVTNRTYERAVKLAEEFEGKPVSFDDLFDQL
HKADIVLSSTGATQFIIKPKDVEEVIRRRKLKPMFFIDIAVPRDIDPKVNDVENVYLYDMDDLQGVVASNLQQRAEEAKK
AEAIIDEEIGQFHKWLSNLEVTPTIVALRSKFEETRKAELEKTLASWKDLPPDGAKRLEALTTAIVNKLLHPPTATLKRV
GQGGRTDLYVDALRTLFELQTGESEAEELGELEE

Specific function: Catalyzes the NADPH-dependent reduction of glutamyl- tRNA(Glu) to glutamate 1-semialdehyde (GSA)

COG id: COG0373

COG function: function code H; Glutamyl-tRNA reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamyl-tRNA reductase family

Homologues:

Organism=Escherichia coli, GI1787461, Length=416, Percent_Identity=41.1057692307692, Blast_Score=312, Evalue=3e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM1_GEOMG (Q39QM8)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_386171.1
- ProteinModelPortal:   Q39QM8
- SMR:   Q39QM8
- STRING:   Q39QM8
- GeneID:   3740949
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_3233
- NMPDR:   fig|269799.3.peg.2468
- eggNOG:   COG0373
- HOGENOM:   HBG732626
- OMA:   GPILNRL
- PhylomeDB:   Q39QM8
- ProtClustDB:   PRK00045
- BioCyc:   GMET269799:GMET_3233-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00087
- InterPro:   IPR000343
- InterPro:   IPR015896
- InterPro:   IPR015895
- InterPro:   IPR016040
- InterPro:   IPR018214
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000445
- TIGRFAMs:   TIGR01035

Pfam domain/function: PF00745 GlutR_dimer; PF05201 GlutR_N; PF01488 Shikimate_DH; SSF69075 4pyrrol_synth_GluRdtase_C; SSF69742 GlutR

EC number: =1.2.1.70

Molecular weight: Translated: 48227; Mature: 48227

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00747 GLUTR

Important sites: ACT_SITE 50-50 BINDING 109-109 BINDING 120-120

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIVVVGLSHKTASVDIREKVAFAPTQMEKPLRALLAIEDIAEAVIVSTCNRVEVYISTR
CCEEEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
DIAGGMARIKRFLADYHGISPEILEPHLYAHHGEAAIRHVFRVASSLDSMVVGEPQILGQ
HHHHHHHHHHHHHHHHCCCCHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHH
IKTAYGYAAEFKTSGIILNRFLHKAFSVAKRVRTETKIASSAVSVSFAAVELARKIFGNL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SDKTVMLIGAGEMCELAAKHFINNGVRGVMVTNRTYERAVKLAEEFEGKPVSFDDLFDQL
CCCEEEEEECCHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHCCCCCCHHHHHHHH
HKADIVLSSTGATQFIIKPKDVEEVIRRRKLKPMFFIDIAVPRDIDPKVNDVENVYLYDM
HHHCEEEECCCCEEEEECCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEEEEC
DDLQGVVASNLQQRAEEAKKAEAIIDEEIGQFHKWLSNLEVTPTIVALRSKFEETRKAEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
EKTLASWKDLPPDGAKRLEALTTAIVNKLLHPPTATLKRVGQGGRTDLYVDALRTLFELQ
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHH
TGESEAEELGELEE
CCCHHHHHCCCCCC
>Mature Secondary Structure
MNIVVVGLSHKTASVDIREKVAFAPTQMEKPLRALLAIEDIAEAVIVSTCNRVEVYISTR
CCEEEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
DIAGGMARIKRFLADYHGISPEILEPHLYAHHGEAAIRHVFRVASSLDSMVVGEPQILGQ
HHHHHHHHHHHHHHHHCCCCHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHH
IKTAYGYAAEFKTSGIILNRFLHKAFSVAKRVRTETKIASSAVSVSFAAVELARKIFGNL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SDKTVMLIGAGEMCELAAKHFINNGVRGVMVTNRTYERAVKLAEEFEGKPVSFDDLFDQL
CCCEEEEEECCHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHCCCCCCHHHHHHHH
HKADIVLSSTGATQFIIKPKDVEEVIRRRKLKPMFFIDIAVPRDIDPKVNDVENVYLYDM
HHHCEEEECCCCEEEEECCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEEEEC
DDLQGVVASNLQQRAEEAKKAEAIIDEEIGQFHKWLSNLEVTPTIVALRSKFEETRKAEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
EKTLASWKDLPPDGAKRLEALTTAIVNKLLHPPTATLKRVGQGGRTDLYVDALRTLFELQ
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHH
TGESEAEELGELEE
CCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA