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The map label for this gene is zraR [H]

Identifier: 78224349

GI number: 78224349

Start: 3553803

End: 3555191

Strand: Direct

Name: zraR [H]

Synonym: Gmet_3158

Alternate gene names: 78224349

Gene position: 3553803-3555191 (Clockwise)

Preceding gene: 78224348

Following gene: 78224350

Centisome position: 88.9

GC content: 64.65

Gene sequence:

>1389_bases
ATGACCGGAACCATTCTCATAGTTGACGACGAGCGGGCACAGCGGGAGATCATCCAGACGATCCTGGAGGGAGAAGGGTA
CCGCGTGGCAGCGGCTCCCGGAGGGCGCGAGGCCCTGGAGCAGCTCGAAACGAGCGAGTTCGACGTGATCCTCACGGACC
TGAAGATGCAGGGGATGTCGGGGATGGAGCTCCTGGAGCGGGTCCTGGAGGCCGACCCCCTCCAGTGCGCCATCATGATG
ACCGCCCACGGCACCGTCGACTCGGCGGTGGAGGCCATGAAAAAGGGGGCCTTCGATTATCTGGAGAAGCCCCTGGAGCG
GGACAACCTCCTCCTCACCCTGCGCCGGGCCTTCGAGCGGGTGAATCTCCTGCGGGAGAACCGGGTCCTCCAGAAGCGGC
TCAAGGAGACGAGCAGCATCCCCAACATCATCGGCGAACACCCGAAGATGCGGGAGGTCTTCCGGATCGTCACCAAGATC
GCCCCCACCGCCTCAACAGTCCTCATCTACGGCGAGTCGGGGACCGGCAAGGAGCTGGTGGCCAAGGCGATCCACGACGG
GAGCCCCCGGAAAGACAAGCCTTTCTTCGCCATAAACTGCGCCGCCATCCCCGAGAGCCTCATGGAGAGCGAGCTCTTCG
GCCACGAGAAAGGGGCCTTCACCGGCGCCAGCTCCCGGGAGCTGGGAATTTTCGAGGCGGCCACCGGTGGGACGGTTTTC
CTGGACGAAATCGGCGAGATGAGTGTGGCCATGCAGGCAAAGCTCCTGCGGGCCATCCAGTCGAAGGAGATCCGCCGGGT
CGGGGGAAAGGTGAACATCCCCGTGGACGTGCGGATCATTTCCGCCACCAACCGGGACCTGGAGGCGGAGATCAAACGGG
GGGGCTTCCGGGAGGACCTCTTCTACCGCCTGAACGTGATCCGGATCACTCTCCCCCCCCTGCGGGAGCGGGGGAGCGAC
ATCGCGGCCCTGGCCGACTTCTTCGTGAAAAAGTGCAGCGCCCAGGCGGGAGTCACCGTCAGGGGGATCGCCAAACCGGC
CCTGAAGCTCATCATGGACTACAGCTGGCCCGGCAACGTCCGGCAGTTGGAATCGGTCATCGAGCGGGGGGCGCTCATGG
CGGAAAGCGACCTGATCCAGCCCGAGGATCTGCCGGCCGAGATCACCGAAGGGCTCTCCCGGGCAGGATGGCTCCCCTTC
GAGCTTCCCCCCGACGGAATCCAGTTCGATGAGCTGGAGCGCAACCTCATCATCAAGGCCATGGAACGGGCCGACTGGGT
CATCGCCAAGGCGGCCCCGCTTCTGGGCATGAGCTACAAGACGCTTCAGTACCGGCTGGAGAAGTTCGAGATAGGCCGGG
ACCGGGGAACGGGGAACGGGGACCAGTAA

Upstream 100 bases:

>100_bases
AGGGATCTAGCATGGGAAGAGGGATTGGGAGCAAAATCAGGTTTTACGTTACTGGTCCCCGGTCCCGGCCTTTGCCGGTC
CCTGTCTCCGAAGGAGACCA

Downstream 100 bases:

>100_bases
GAACCTTACAATCTTTTCAGCACATACGAAAACGCCATCAACCAGTCAAATACGGAGGATGCAACGACCGACCGTGGATC
AAGGGTTTTCCCGGTCCCCG

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MTGTILIVDDERAQREIIQTILEGEGYRVAAAPGGREALEQLETSEFDVILTDLKMQGMSGMELLERVLEADPLQCAIMM
TAHGTVDSAVEAMKKGAFDYLEKPLERDNLLLTLRRAFERVNLLRENRVLQKRLKETSSIPNIIGEHPKMREVFRIVTKI
APTASTVLIYGESGTGKELVAKAIHDGSPRKDKPFFAINCAAIPESLMESELFGHEKGAFTGASSRELGIFEAATGGTVF
LDEIGEMSVAMQAKLLRAIQSKEIRRVGGKVNIPVDVRIISATNRDLEAEIKRGGFREDLFYRLNVIRITLPPLRERGSD
IAALADFFVKKCSAQAGVTVRGIAKPALKLIMDYSWPGNVRQLESVIERGALMAESDLIQPEDLPAEITEGLSRAGWLPF
ELPPDGIQFDELERNLIIKAMERADWVIAKAAPLLGMSYKTLQYRLEKFEIGRDRGTGNGDQ

Sequences:

>Translated_462_residues
MTGTILIVDDERAQREIIQTILEGEGYRVAAAPGGREALEQLETSEFDVILTDLKMQGMSGMELLERVLEADPLQCAIMM
TAHGTVDSAVEAMKKGAFDYLEKPLERDNLLLTLRRAFERVNLLRENRVLQKRLKETSSIPNIIGEHPKMREVFRIVTKI
APTASTVLIYGESGTGKELVAKAIHDGSPRKDKPFFAINCAAIPESLMESELFGHEKGAFTGASSRELGIFEAATGGTVF
LDEIGEMSVAMQAKLLRAIQSKEIRRVGGKVNIPVDVRIISATNRDLEAEIKRGGFREDLFYRLNVIRITLPPLRERGSD
IAALADFFVKKCSAQAGVTVRGIAKPALKLIMDYSWPGNVRQLESVIERGALMAESDLIQPEDLPAEITEGLSRAGWLPF
ELPPDGIQFDELERNLIIKAMERADWVIAKAAPLLGMSYKTLQYRLEKFEIGRDRGTGNGDQ
>Mature_461_residues
TGTILIVDDERAQREIIQTILEGEGYRVAAAPGGREALEQLETSEFDVILTDLKMQGMSGMELLERVLEADPLQCAIMMT
AHGTVDSAVEAMKKGAFDYLEKPLERDNLLLTLRRAFERVNLLRENRVLQKRLKETSSIPNIIGEHPKMREVFRIVTKIA
PTASTVLIYGESGTGKELVAKAIHDGSPRKDKPFFAINCAAIPESLMESELFGHEKGAFTGASSRELGIFEAATGGTVFL
DEIGEMSVAMQAKLLRAIQSKEIRRVGGKVNIPVDVRIISATNRDLEAEIKRGGFREDLFYRLNVIRITLPPLRERGSDI
AALADFFVKKCSAQAGVTVRGIAKPALKLIMDYSWPGNVRQLESVIERGALMAESDLIQPEDLPAEITEGLSRAGWLPFE
LPPDGIQFDELERNLIIKAMERADWVIAKAAPLLGMSYKTLQYRLEKFEIGRDRGTGNGDQ

Specific function: Member of the two-component regulatory system zraS/zraR. When activated by zraS it acts in conjunction with sigma-54 to regulate the expression of zraP. Positively autoregulates the expression of the zraSR operon [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=452, Percent_Identity=43.5840707964602, Blast_Score=371, Evalue=1e-104,
Organism=Escherichia coli, GI1790437, Length=444, Percent_Identity=43.9189189189189, Blast_Score=367, Evalue=1e-102,
Organism=Escherichia coli, GI1790299, Length=469, Percent_Identity=41.1513859275053, Blast_Score=338, Evalue=5e-94,
Organism=Escherichia coli, GI1788905, Length=375, Percent_Identity=42.6666666666667, Blast_Score=308, Evalue=4e-85,
Organism=Escherichia coli, GI87082117, Length=341, Percent_Identity=44.574780058651, Blast_Score=275, Evalue=4e-75,
Organism=Escherichia coli, GI1789233, Length=322, Percent_Identity=45.3416149068323, Blast_Score=264, Evalue=1e-71,
Organism=Escherichia coli, GI87082152, Length=235, Percent_Identity=51.4893617021277, Blast_Score=245, Evalue=5e-66,
Organism=Escherichia coli, GI1789087, Length=357, Percent_Identity=39.7759103641457, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI1786524, Length=245, Percent_Identity=47.3469387755102, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI87081872, Length=320, Percent_Identity=40.3125, Blast_Score=225, Evalue=4e-60,
Organism=Escherichia coli, GI1787583, Length=262, Percent_Identity=43.5114503816794, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI87081858, Length=317, Percent_Identity=29.6529968454259, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1789828, Length=256, Percent_Identity=33.59375, Blast_Score=136, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 51213; Mature: 51082

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGTILIVDDERAQREIIQTILEGEGYRVAAAPGGREALEQLETSEFDVILTDLKMQGMS
CCCEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCEEEEECHHHCCCC
GMELLERVLEADPLQCAIMMTAHGTVDSAVEAMKKGAFDYLEKPLERDNLLLTLRRAFER
HHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHH
VNLLRENRVLQKRLKETSSIPNIIGEHPKMREVFRIVTKIAPTASTVLIYGESGTGKELV
HHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHH
AKAIHDGSPRKDKPFFAINCAAIPESLMESELFGHEKGAFTGASSRELGIFEAATGGTVF
HHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCEEE
LDEIGEMSVAMQAKLLRAIQSKEIRRVGGKVNIPVDVRIISATNRDLEAEIKRGGFREDL
EHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCEEEEEEECCCCCHHHHHHCCCCHHHH
FYRLNVIRITLPPLRERGSDIAALADFFVKKCSAQAGVTVRGIAKPALKLIMDYSWPGNV
HEEEEEEEEECCCHHHCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCCCCCH
RQLESVIERGALMAESDLIQPEDLPAEITEGLSRAGWLPFELPPDGIQFDELERNLIIKA
HHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
MERADWVIAKAAPLLGMSYKTLQYRLEKFEIGRDRGTGNGDQ
HHHHCEEHHHHCHHHCCCHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TGTILIVDDERAQREIIQTILEGEGYRVAAAPGGREALEQLETSEFDVILTDLKMQGMS
CCEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCEEEEECHHHCCCC
GMELLERVLEADPLQCAIMMTAHGTVDSAVEAMKKGAFDYLEKPLERDNLLLTLRRAFER
HHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHH
VNLLRENRVLQKRLKETSSIPNIIGEHPKMREVFRIVTKIAPTASTVLIYGESGTGKELV
HHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHH
AKAIHDGSPRKDKPFFAINCAAIPESLMESELFGHEKGAFTGASSRELGIFEAATGGTVF
HHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCEEE
LDEIGEMSVAMQAKLLRAIQSKEIRRVGGKVNIPVDVRIISATNRDLEAEIKRGGFREDL
EHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCEEEEEEECCCCCHHHHHHCCCCHHHH
FYRLNVIRITLPPLRERGSDIAALADFFVKKCSAQAGVTVRGIAKPALKLIMDYSWPGNV
HEEEEEEEEECCCHHHCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCCCCCH
RQLESVIERGALMAESDLIQPEDLPAEITEGLSRAGWLPFELPPDGIQFDELERNLIIKA
HHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
MERADWVIAKAAPLLGMSYKTLQYRLEKFEIGRDRGTGNGDQ
HHHHCEEHHHHCHHHCCCHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11243806 [H]