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The map label for this gene is trmB [H]

Identifier: 78224227

GI number: 78224227

Start: 3419441

End: 3420154

Strand: Reverse

Name: trmB [H]

Synonym: Gmet_3034

Alternate gene names: 78224227

Gene position: 3420154-3419441 (Counterclockwise)

Preceding gene: 78224229

Following gene: 78224226

Centisome position: 85.56

GC content: 60.92

Gene sequence:

>714_bases
ATGGCAAGAAAAGCGCTTCCGGGCTTCCGGGCTTTTGTGGTATGGTACGCCCACTTCAACCCCGAGGAACCGACAAGAAT
GCAGCGCATGATCGAAATCTCTTCACCCTTTTTCCTGAACCCTGACGAACTGTCAACGCCCGCCGATTGGGCCGCCGTCT
TCGGCAACGCCAATCCCCTGGCCCTGGAGATCGGCTGCGGCATCGGCGACTTCATCGCCAGGACAGCCGCCGACAACCCC
GGCACCAACTATATCGCCATCGACTTTTACAACAAGGGGTGCGACAAGACCTGCCGCCGCCTCGAACGGCTGGCTATTCC
CAACGTCCGGGTCGTGCGGGACGAGGCCCGCAAGTTCATCGTCGAGCGCATTCCCAAGGGTTCCCTCTGTGCCGTCCACA
TCAACTGTCCCGACCCGTGGCCCAAGATGCGCCACCGCAAGCGCCGCCTCGTGAACCGGGAGTTTGCGGCGTTCATCCGC
GAGTATCTGGCACCGGGGGGCGATTTCTACTTTGCCACCGACTTCGATGACTACGGCATCGACGTGGCGGAGTTCATGCC
GGGAGTGGAGGGGTACGCGAATATGCTCGCCCCGGACCGTTACCGCCACGAGTTGGAAGGGTACCATCTCTCCAAGTACA
TGATGAAGTTCATGGCCGAGGGGAAGCGGATCTACTTCGTCCACTACCGGAAAACGGCAGAGGGGGCAGCGTGA

Upstream 100 bases:

>100_bases
CCCGTTGCACCAGTTCGGGGAGGTTTAAGTCGGTGAATTTCATCTGGTGTTCCTTTTTTTCGTTATTTCAACCAGATAGC
TATAGCACAGGAGGTCGGGA

Downstream 100 bases:

>100_bases
GCGGCGAGAAGCGCTATCTGACCGCCGGCCTCCCCGGCACCGGAGGCACCATCAAGGAGACCGCCGACGATTTCGTGGTG
GAGGAGATTCCCCTCTACCT

Product: putative methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MARKALPGFRAFVVWYAHFNPEEPTRMQRMIEISSPFFLNPDELSTPADWAAVFGNANPLALEIGCGIGDFIARTAADNP
GTNYIAIDFYNKGCDKTCRRLERLAIPNVRVVRDEARKFIVERIPKGSLCAVHINCPDPWPKMRHRKRRLVNREFAAFIR
EYLAPGGDFYFATDFDDYGIDVAEFMPGVEGYANMLAPDRYRHELEGYHLSKYMMKFMAEGKRIYFVHYRKTAEGAA

Sequences:

>Translated_237_residues
MARKALPGFRAFVVWYAHFNPEEPTRMQRMIEISSPFFLNPDELSTPADWAAVFGNANPLALEIGCGIGDFIARTAADNP
GTNYIAIDFYNKGCDKTCRRLERLAIPNVRVVRDEARKFIVERIPKGSLCAVHINCPDPWPKMRHRKRRLVNREFAAFIR
EYLAPGGDFYFATDFDDYGIDVAEFMPGVEGYANMLAPDRYRHELEGYHLSKYMMKFMAEGKRIYFVHYRKTAEGAA
>Mature_236_residues
ARKALPGFRAFVVWYAHFNPEEPTRMQRMIEISSPFFLNPDELSTPADWAAVFGNANPLALEIGCGIGDFIARTAADNPG
TNYIAIDFYNKGCDKTCRRLERLAIPNVRVVRDEARKFIVERIPKGSLCAVHINCPDPWPKMRHRKRRLVNREFAAFIRE
YLAPGGDFYFATDFDDYGIDVAEFMPGVEGYANMLAPDRYRHELEGYHLSKYMMKFMAEGKRIYFVHYRKTAEGAA

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=153, Percent_Identity=33.9869281045752, Blast_Score=104, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 27237; Mature: 27105

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKALPGFRAFVVWYAHFNPEEPTRMQRMIEISSPFFLNPDELSTPADWAAVFGNANPL
CCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHCCCCEECHHHCCCCHHHHHHHCCCCCE
ALEIGCGIGDFIARTAADNPGTNYIAIDFYNKGCDKTCRRLERLAIPNVRVVRDEARKFI
EEEECCCHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEHHHHHHHHH
VERIPKGSLCAVHINCPDPWPKMRHRKRRLVNREFAAFIREYLAPGGDFYFATDFDDYGI
HHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
DVAEFMPGVEGYANMLAPDRYRHELEGYHLSKYMMKFMAEGKRIYFVHYRKTAEGAA
CHHHHCCCHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCC
>Mature Secondary Structure 
ARKALPGFRAFVVWYAHFNPEEPTRMQRMIEISSPFFLNPDELSTPADWAAVFGNANPL
CCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHCCCCEECHHHCCCCHHHHHHHCCCCCE
ALEIGCGIGDFIARTAADNPGTNYIAIDFYNKGCDKTCRRLERLAIPNVRVVRDEARKFI
EEEECCCHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEHHHHHHHHH
VERIPKGSLCAVHINCPDPWPKMRHRKRRLVNREFAAFIREYLAPGGDFYFATDFDDYGI
HHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
DVAEFMPGVEGYANMLAPDRYRHELEGYHLSKYMMKFMAEGKRIYFVHYRKTAEGAA
CHHHHCCCHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA