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The map label for this gene is pilJ [H]

Identifier: 78224134

GI number: 78224134

Start: 3323591

End: 3325285

Strand: Direct

Name: pilJ [H]

Synonym: Gmet_2938

Alternate gene names: 78224134

Gene position: 3323591-3325285 (Clockwise)

Preceding gene: 78224133

Following gene: 78224140

Centisome position: 83.14

GC content: 61.83

Gene sequence:

>1695_bases
ATGACCAAAAACCTGCGCCTCGGCTCAAAACTGATCCGCTCCTCGCTCCTTGCGGGCTTCGTCATTGCCCTGGTGGGCAT
CATCTGCGCCTATAATGCCGGCGGCACCCCCGCGACCCAGGGATACGCCCGGCTAACCCTGGGCATCTCCCTCATCAATG
CGCTTCTTTTTCTCTTTGTCCTCTGGATGTTCACTGCCAGAAAAATGGTCGTCCGTGTCGGCAAATTGGCCGGTGCCATG
GATCGTGGCGCCGAGGGCGATCTGACAATATCTGTACGGGATGATACCGAGGACGAACTGGGACTGCTGACTACCAACTT
CAACGCCATGCTCCAGCACCTGGCCGCGACAGTCACCAAGGTGAAGTCATCGGTGACAGAACTGCGTACCATTGCTGCAA
CGGTACGGGATGCGGCCGACCGGGGCGTTGCCACCGCGGAGATACAGGCCGAGGCGGTCCAGGGGACCACAGACGGCATC
CATGCCATCGACCGGTCGGTCACTGACGTGGCCCTGTCCGTGGGGCAGCTTTCCCGCACCGCCATGGAGAACTCCTCTTC
CATCCTCCAGATGTCGGCAAGCATCGAGGAAGTGGCCGACCACGTGGAATCCCTGGCCAATGCCGTTGAAGAGGTGAGCT
CTTCCATCATCCAGATGGCGACTGCCGAGAAGGAGATAGGCTCCAACGCCGCCACCCTCATGAACGATGCCATGCGTACG
GCGTCGCTGGTTGCCGAGCTTGACACCTCCATCAAGCAGATCGAGAAGAGCGCCGTGGAGACCGCGGCAATCTCCGAGGA
AGTGCTGAGGGATGCCGAGCAGGGGCGCGAGGCTGTGGAGAGCACCATCTCCGGCATCGACGAGATCCGTCGTTCCTCAC
GCTCCGTGGGTGAAACCATCGAAACCCTGTCACAGCGGGCGGGCCACATCGGCACGATCATTTCGGTCATCAACGACATC
GCGGAGCAGACCAAGCTTCTGGCCCTCAACGCCTCCATCATCGCGGCCCAGGCAGGGGAGCACGGCAAGGGATTCGCGGT
GGTTGCCAACGAGATCAAGGAACTTGCCAAGCGGACCACCAGCTCTACCGGAGAGATCGGCACCATTATCATGGGGCTGC
GGGAAGAGAGCGAGCGCGCGGTTGCGGCCAACAAGCATGCGGAACTGCGGATCACTGAAGGGGAGAAACTTTCGTACCGT
TCGGGCGAGGCTCTCGACAAGATCGTCGACGGCGTCAAAATGGCTGCGGGGCAGGTGAATGACATCGCCCGCACCACCGT
GGAGCAGGCCCAGAGCAGCGAGCACATGCGGAACGCCGTGGAGCGGGTGGCGGGAATGGTGGAGCAGATCGCCCGGGCCA
CCGAAGAGCAGACCTACGGCACAGGGCTCATCATGGATGCCGTAGCCCGTATGAAGAGCGTCACGTCTCAGGTGCGGCAG
TCCAGCCAGGAGCAGCGCAACGCGAGCACCCTCATCGTCAGGTCCAGCGAGGGGATCACCGGGATGATCGCCACGATCCG
CCAGGCCTGCCAGGTCCAGACCCAGAGCACCGAAAAGATTGTCCAGGCGGTGGACAACATGGCGCGGACATCCGAGGGGA
ATGTGGAGACCACCCGGGTCATGGAAAATGCCGTCAACGGCCTCACCCGCCAGATCGACGAACTGAACGAGGCAATGACC
GGGTTCCGGGTTTAG

Upstream 100 bases:

>100_bases
CCTTTGCATATTTTAGGAGATGATCTGATTTCGTGCATCAATCTCTGGACTGATTCATCCCCGATAAATCCTGGGTACGC
CATCGCAGAAGGAGACCCCG

Downstream 100 bases:

>100_bases
GGCTTGCTATCAGTCGTCGGCCCGGTAGCCGATCCTGACCGCTCCCCAGTGTTTCCCGCCGACTGTTATGGGCGTTGACA
GGTCGTTCATGACCTCCCCC

Product: methyl-accepting chemotaxis sensory transducer

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 564; Mature: 563

Protein sequence:

>564_residues
MTKNLRLGSKLIRSSLLAGFVIALVGIICAYNAGGTPATQGYARLTLGISLINALLFLFVLWMFTARKMVVRVGKLAGAM
DRGAEGDLTISVRDDTEDELGLLTTNFNAMLQHLAATVTKVKSSVTELRTIAATVRDAADRGVATAEIQAEAVQGTTDGI
HAIDRSVTDVALSVGQLSRTAMENSSSILQMSASIEEVADHVESLANAVEEVSSSIIQMATAEKEIGSNAATLMNDAMRT
ASLVAELDTSIKQIEKSAVETAAISEEVLRDAEQGREAVESTISGIDEIRRSSRSVGETIETLSQRAGHIGTIISVINDI
AEQTKLLALNASIIAAQAGEHGKGFAVVANEIKELAKRTTSSTGEIGTIIMGLREESERAVAANKHAELRITEGEKLSYR
SGEALDKIVDGVKMAAGQVNDIARTTVEQAQSSEHMRNAVERVAGMVEQIARATEEQTYGTGLIMDAVARMKSVTSQVRQ
SSQEQRNASTLIVRSSEGITGMIATIRQACQVQTQSTEKIVQAVDNMARTSEGNVETTRVMENAVNGLTRQIDELNEAMT
GFRV

Sequences:

>Translated_564_residues
MTKNLRLGSKLIRSSLLAGFVIALVGIICAYNAGGTPATQGYARLTLGISLINALLFLFVLWMFTARKMVVRVGKLAGAM
DRGAEGDLTISVRDDTEDELGLLTTNFNAMLQHLAATVTKVKSSVTELRTIAATVRDAADRGVATAEIQAEAVQGTTDGI
HAIDRSVTDVALSVGQLSRTAMENSSSILQMSASIEEVADHVESLANAVEEVSSSIIQMATAEKEIGSNAATLMNDAMRT
ASLVAELDTSIKQIEKSAVETAAISEEVLRDAEQGREAVESTISGIDEIRRSSRSVGETIETLSQRAGHIGTIISVINDI
AEQTKLLALNASIIAAQAGEHGKGFAVVANEIKELAKRTTSSTGEIGTIIMGLREESERAVAANKHAELRITEGEKLSYR
SGEALDKIVDGVKMAAGQVNDIARTTVEQAQSSEHMRNAVERVAGMVEQIARATEEQTYGTGLIMDAVARMKSVTSQVRQ
SSQEQRNASTLIVRSSEGITGMIATIRQACQVQTQSTEKIVQAVDNMARTSEGNVETTRVMENAVNGLTRQIDELNEAMT
GFRV
>Mature_563_residues
TKNLRLGSKLIRSSLLAGFVIALVGIICAYNAGGTPATQGYARLTLGISLINALLFLFVLWMFTARKMVVRVGKLAGAMD
RGAEGDLTISVRDDTEDELGLLTTNFNAMLQHLAATVTKVKSSVTELRTIAATVRDAADRGVATAEIQAEAVQGTTDGIH
AIDRSVTDVALSVGQLSRTAMENSSSILQMSASIEEVADHVESLANAVEEVSSSIIQMATAEKEIGSNAATLMNDAMRTA
SLVAELDTSIKQIEKSAVETAAISEEVLRDAEQGREAVESTISGIDEIRRSSRSVGETIETLSQRAGHIGTIISVINDIA
EQTKLLALNASIIAAQAGEHGKGFAVVANEIKELAKRTTSSTGEIGTIIMGLREESERAVAANKHAELRITEGEKLSYRS
GEALDKIVDGVKMAAGQVNDIARTTVEQAQSSEHMRNAVERVAGMVEQIARATEEQTYGTGLIMDAVARMKSVTSQVRQS
SQEQRNASTLIVRSSEGITGMIATIRQACQVQTQSTEKIVQAVDNMARTSEGNVETTRVMENAVNGLTRQIDELNEAMTG
FRV

Specific function: May be a part of a signal-transduction system that regulates twitching motility by controlling pilus function (extension and retraction) [H]

COG id: COG0840

COG function: function code NT; Methyl-accepting chemotaxis protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI1789453, Length=278, Percent_Identity=28.7769784172662, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788195, Length=238, Percent_Identity=30.2521008403361, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI2367378, Length=239, Percent_Identity=32.2175732217573, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1788194, Length=159, Percent_Identity=38.3647798742138, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1787690, Length=239, Percent_Identity=29.2887029288703, Blast_Score=73, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 60172; Mature: 60041

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKNLRLGSKLIRSSLLAGFVIALVGIICAYNAGGTPATQGYARLTLGISLINALLFLFV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LWMFTARKMVVRVGKLAGAMDRGAEGDLTISVRDDTEDELGLLTTNFNAMLQHLAATVTK
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHEEECCHHHHHHHHHHHHHH
VKSSVTELRTIAATVRDAADRGVATAEIQAEAVQGTTDGIHAIDRSVTDVALSVGQLSRT
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
AMENSSSILQMSASIEEVADHVESLANAVEEVSSSIIQMATAEKEIGSNAATLMNDAMRT
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
ASLVAELDTSIKQIEKSAVETAAISEEVLRDAEQGREAVESTISGIDEIRRSSRSVGETI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETLSQRAGHIGTIISVINDIAEQTKLLALNASIIAAQAGEHGKGFAVVANEIKELAKRTT
HHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHEECCCCCCCCHHHHHHHHHHHHHHHC
SSTGEIGTIIMGLREESERAVAANKHAELRITEGEKLSYRSGEALDKIVDGVKMAAGQVN
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCHH
DIARTTVEQAQSSEHMRNAVERVAGMVEQIARATEEQTYGTGLIMDAVARMKSVTSQVRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
SSQEQRNASTLIVRSSEGITGMIATIRQACQVQTQSTEKIVQAVDNMARTSEGNVETTRV
HHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
MENAVNGLTRQIDELNEAMTGFRV
HHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKNLRLGSKLIRSSLLAGFVIALVGIICAYNAGGTPATQGYARLTLGISLINALLFLFV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LWMFTARKMVVRVGKLAGAMDRGAEGDLTISVRDDTEDELGLLTTNFNAMLQHLAATVTK
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHEEECCHHHHHHHHHHHHHH
VKSSVTELRTIAATVRDAADRGVATAEIQAEAVQGTTDGIHAIDRSVTDVALSVGQLSRT
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
AMENSSSILQMSASIEEVADHVESLANAVEEVSSSIIQMATAEKEIGSNAATLMNDAMRT
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
ASLVAELDTSIKQIEKSAVETAAISEEVLRDAEQGREAVESTISGIDEIRRSSRSVGETI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETLSQRAGHIGTIISVINDIAEQTKLLALNASIIAAQAGEHGKGFAVVANEIKELAKRTT
HHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHEECCCCCCCCHHHHHHHHHHHHHHHC
SSTGEIGTIIMGLREESERAVAANKHAELRITEGEKLSYRSGEALDKIVDGVKMAAGQVN
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCHH
DIARTTVEQAQSSEHMRNAVERVAGMVEQIARATEEQTYGTGLIMDAVARMKSVTSQVRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
SSQEQRNASTLIVRSSEGITGMIATIRQACQVQTQSTEKIVQAVDNMARTSEGNVETTRV
HHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
MENAVNGLTRQIDELNEAMTGFRV
HHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7908398; 10984043 [H]