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The map label for this gene is thiDE [H]

Identifier: 78224105

GI number: 78224105

Start: 3292967

End: 3294460

Strand: Direct

Name: thiDE [H]

Synonym: Gmet_2909

Alternate gene names: 78224105

Gene position: 3292967-3294460 (Clockwise)

Preceding gene: 78224079

Following gene: 78224106

Centisome position: 82.38

GC content: 68.54

Gene sequence:

>1494_bases
ATGGGCAGCAACGGCCACAATCTCCGGCTGGTCGTCAATCGCGACAAGCAGGACTCCCCCATCCGGGGGGTGTACCTGGT
CACCGACCACGGCGACCGTCTCGCGGAGCGGGTGGAGGCGGCAATCGACGGCGGGGTCCGCATCGTCCAATACCGGAACA
AGGGAAAGGACCGGGCCCAGCGCTACGCCGTGGGTGAGGAACTACGGGACCTCTGCAGCCGGCGGGGGGTGATCTTCATC
GTCAACGACGACCTGGATCTGGCGCTGCAACTCAAGGCCGATGGCATCCACCTGGGGCAGGAGGACGGCGACCCCCGTAT
TGCCCGGCGCGAACTGGGACCGGGTAGAATCATCGGCGTCTCCACCCATACCATGGAAGAAGCCCTGGCGGCCCAGGCGG
CGGGGGCCGACTACATCGGCTTCGGCGCCATGTTCCCCACCAACAGCAAGGAGATCGGGCACCTGGCCGGGCCCGAGGGG
CTCGCCGCCATCCGCAGCCGAATCATGATCCCCATTGTTGCCATCGGCGGCATCAGCCGGGACAACGGCCCACGGGTGGT
CGACGCCGGAGCCGACGCCCTGGCGGTCATCTCGGCGGTTCTCGCCCATCCGGACCCGTTCCTGGCCGCGACGGAGCTGG
GCCTTCTCTTCAACCGGCGCGCACCACATCCCCGGGGGGCTGTGCTCACCATTGCAGGAAGTGACTCGGGGGGCGGAGCC
GGCATCCAGGCCGACCTCAAGACCGCGACACTGCTGGGGAGCTACGGCTCATCGGTCATCACGGCGCTCACTGCCCAGAA
TACCCGGGGGGTCTCCGGCATTCACGGCGTCCCCCCCTCCTTCGTGGCCGAGCAGCTCGATGCGGTCCTTTCCGACATCC
CCGTGGACACGGTAAAGACCGGCATGCTCTTCTCTGCCGAAATCATCGCCACCGTGGCCGACAAGCTGGCCGAGTACCGG
AAGCGGATCGTGGTGGTGGACCCGGTCATGGTCGCCAAGGGAGGCGCCCCCCTCATCGACCGGGGAGCCGTGAACGTCCT
CAAGGACCGCCTCATGCCCCGCACCTACCTCCTCACCCCCAACATCCCCGAGGCGGAACGGCTCACGGGGCTCACCATCG
TCGACGAAGAGGGAATGCAGGAGGCGGCCCGGCGCCTCTTCCGTCTCGGTGCCCGCAACGTCCTCGTCAAGGGGGGGCAT
CTGGTGGCCGGTGATGCGGTGGACATCCTCTTCGACGGCTCCGCCTTCCACCGTTTCACCGCGCCCCGCATCCTCAGCAA
GAACACCCACGGAACCGGCTGCACCTACGCCGCGGCCATCGCCACCTACCTGGCCCAGGGAGAGCCGCTCCGGGAGGCCG
TTGGCCGCGCCAAGGAGTTCGTCACCGCCGCAATCCGCCTGGGCCAGCCCCTGGGCCGGGGGCACGGACCGGTGAACCAC
CTCCTCGCCGCGCTTGAAGTCGGGGACCAGGGACCGGGGACCGGGGACCGGTAA

Upstream 100 bases:

>100_bases
CGGGTAATGCCGGCGTAGGGAAGCGGCCAGAGACCTCGTTTACCTCGTTGAGTTCTGGGACCGTGACCATCGTCACGGTT
TTTTTGTTTGGGAGATCACC

Downstream 100 bases:

>100_bases
TACCGTTTTGTCCAACCAGATCCGTGAGGCGAGGGAGCCTGACGGAGCGAATGCGCCCTCTTTCGTCGCCGCTCTTGGCG
ACATGGAGCGGCCACAGCCG

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MGSNGHNLRLVVNRDKQDSPIRGVYLVTDHGDRLAERVEAAIDGGVRIVQYRNKGKDRAQRYAVGEELRDLCSRRGVIFI
VNDDLDLALQLKADGIHLGQEDGDPRIARRELGPGRIIGVSTHTMEEALAAQAAGADYIGFGAMFPTNSKEIGHLAGPEG
LAAIRSRIMIPIVAIGGISRDNGPRVVDAGADALAVISAVLAHPDPFLAATELGLLFNRRAPHPRGAVLTIAGSDSGGGA
GIQADLKTATLLGSYGSSVITALTAQNTRGVSGIHGVPPSFVAEQLDAVLSDIPVDTVKTGMLFSAEIIATVADKLAEYR
KRIVVVDPVMVAKGGAPLIDRGAVNVLKDRLMPRTYLLTPNIPEAERLTGLTIVDEEGMQEAARRLFRLGARNVLVKGGH
LVAGDAVDILFDGSAFHRFTAPRILSKNTHGTGCTYAAAIATYLAQGEPLREAVGRAKEFVTAAIRLGQPLGRGHGPVNH
LLAALEVGDQGPGTGDR

Sequences:

>Translated_497_residues
MGSNGHNLRLVVNRDKQDSPIRGVYLVTDHGDRLAERVEAAIDGGVRIVQYRNKGKDRAQRYAVGEELRDLCSRRGVIFI
VNDDLDLALQLKADGIHLGQEDGDPRIARRELGPGRIIGVSTHTMEEALAAQAAGADYIGFGAMFPTNSKEIGHLAGPEG
LAAIRSRIMIPIVAIGGISRDNGPRVVDAGADALAVISAVLAHPDPFLAATELGLLFNRRAPHPRGAVLTIAGSDSGGGA
GIQADLKTATLLGSYGSSVITALTAQNTRGVSGIHGVPPSFVAEQLDAVLSDIPVDTVKTGMLFSAEIIATVADKLAEYR
KRIVVVDPVMVAKGGAPLIDRGAVNVLKDRLMPRTYLLTPNIPEAERLTGLTIVDEEGMQEAARRLFRLGARNVLVKGGH
LVAGDAVDILFDGSAFHRFTAPRILSKNTHGTGCTYAAAIATYLAQGEPLREAVGRAKEFVTAAIRLGQPLGRGHGPVNH
LLAALEVGDQGPGTGDR
>Mature_496_residues
GSNGHNLRLVVNRDKQDSPIRGVYLVTDHGDRLAERVEAAIDGGVRIVQYRNKGKDRAQRYAVGEELRDLCSRRGVIFIV
NDDLDLALQLKADGIHLGQEDGDPRIARRELGPGRIIGVSTHTMEEALAAQAAGADYIGFGAMFPTNSKEIGHLAGPEGL
AAIRSRIMIPIVAIGGISRDNGPRVVDAGADALAVISAVLAHPDPFLAATELGLLFNRRAPHPRGAVLTIAGSDSGGGAG
IQADLKTATLLGSYGSSVITALTAQNTRGVSGIHGVPPSFVAEQLDAVLSDIPVDTVKTGMLFSAEIIATVADKLAEYRK
RIVVVDPVMVAKGGAPLIDRGAVNVLKDRLMPRTYLLTPNIPEAERLTGLTIVDEEGMQEAARRLFRLGARNVLVKGGHL
VAGDAVDILFDGSAFHRFTAPRILSKNTHGTGCTYAAAIATYLAQGEPLREAVGRAKEFVTAAIRLGQPLGRGHGPVNHL
LAALEVGDQGPGTGDR

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=258, Percent_Identity=48.8372093023256, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI1790426, Length=199, Percent_Identity=34.1708542713568, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6325378, Length=295, Percent_Identity=34.9152542372881, Blast_Score=152, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6324997, Length=296, Percent_Identity=37.1621621621622, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6324517, Length=301, Percent_Identity=35.8803986710963, Blast_Score=138, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6325042, Length=232, Percent_Identity=32.3275862068966, Blast_Score=105, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF08543 Phos_pyr_kin; PF02581 TMP-TENI [H]

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 52392; Mature: 52260

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSNGHNLRLVVNRDKQDSPIRGVYLVTDHGDRLAERVEAAIDGGVRIVQYRNKGKDRAQ
CCCCCCEEEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCHHHHH
RYAVGEELRDLCSRRGVIFIVNDDLDLALQLKADGIHLGQEDGDPRIARRELGPGRIIGV
HHHHHHHHHHHHHCCCEEEEEECCCCEEEEEEECCEECCCCCCCCCHHHHCCCCCCEEEE
STHTMEEALAAQAAGADYIGFGAMFPTNSKEIGHLAGPEGLAAIRSRIMIPIVAIGGISR
CHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEEEEECCCCC
DNGPRVVDAGADALAVISAVLAHPDPFLAATELGLLFNRRAPHPRGAVLTIAGSDSGGGA
CCCCEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCC
GIQADLKTATLLGSYGSSVITALTAQNTRGVSGIHGVPPSFVAEQLDAVLSDIPVDTVKT
CEEECHHHHHHHHHCCCHHEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHH
GMLFSAEIIATVADKLAEYRKRIVVVDPVMVAKGGAPLIDRGAVNVLKDRLMPRTYLLTP
CHHHHHHHHHHHHHHHHHHHCCEEEECCEEEECCCCCCCCCHHHHHHHHHCCCCEEEECC
NIPEAERLTGLTIVDEEGMQEAARRLFRLGARNVLVKGGHLVAGDAVDILFDGSAFHRFT
CCCCHHHHCCEEEECHHHHHHHHHHHHHHCCCCEEEECCEEEECCEEEEEECCCHHHHCC
APRILSKNTHGTGCTYAAAIATYLAQGEPLREAVGRAKEFVTAAIRLGQPLGRGHGPVNH
CCHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
LLAALEVGDQGPGTGDR
HHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
GSNGHNLRLVVNRDKQDSPIRGVYLVTDHGDRLAERVEAAIDGGVRIVQYRNKGKDRAQ
CCCCCEEEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCHHHHH
RYAVGEELRDLCSRRGVIFIVNDDLDLALQLKADGIHLGQEDGDPRIARRELGPGRIIGV
HHHHHHHHHHHHHCCCEEEEEECCCCEEEEEEECCEECCCCCCCCCHHHHCCCCCCEEEE
STHTMEEALAAQAAGADYIGFGAMFPTNSKEIGHLAGPEGLAAIRSRIMIPIVAIGGISR
CHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEEEEECCCCC
DNGPRVVDAGADALAVISAVLAHPDPFLAATELGLLFNRRAPHPRGAVLTIAGSDSGGGA
CCCCEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCC
GIQADLKTATLLGSYGSSVITALTAQNTRGVSGIHGVPPSFVAEQLDAVLSDIPVDTVKT
CEEECHHHHHHHHHCCCHHEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHH
GMLFSAEIIATVADKLAEYRKRIVVVDPVMVAKGGAPLIDRGAVNVLKDRLMPRTYLLTP
CHHHHHHHHHHHHHHHHHHHCCEEEECCEEEECCCCCCCCCHHHHHHHHHCCCCEEEECC
NIPEAERLTGLTIVDEEGMQEAARRLFRLGARNVLVKGGHLVAGDAVDILFDGSAFHRFT
CCCCHHHHCCEEEECHHHHHHHHHHHHHHCCCCEEEECCEEEECCEEEEEECCCHHHHCC
APRILSKNTHGTGCTYAAAIATYLAQGEPLREAVGRAKEFVTAAIRLGQPLGRGHGPVNH
CCHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
LLAALEVGDQGPGTGDR
HHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA