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The map label for this gene is thiC2

Identifier: 78224106

GI number: 78224106

Start: 3294701

End: 3296011

Strand: Direct

Name: thiC2

Synonym: Gmet_2910

Alternate gene names: 78224106

Gene position: 3294701-3296011 (Clockwise)

Preceding gene: 78224105

Following gene: 304570573

Centisome position: 82.42

GC content: 65.98

Gene sequence:

>1311_bases
ATGGCAACGACGCAGCTTGAATACGCCCGCCAGGGAATCATCACCGACAAAATGAAGGAAGCCGCTCTGGCCGAGGGGGT
TTCCCCCGAGTTCATCCGCGAGGGGATCGCCGCCGGCACCATCATCATCTGTCACAACATCAAGCACGGCAATGGCCGCC
CCCTGGCCGTGGGAAAAGGGCTCCGCACCAAGGTGAACGCCAACATCGGCACCTCGGCCGATGACACGGACATTACCAAG
GAGCTGGAAAAGGCCCGCGTTGCGGTCCGCCACGGCGCCGACGCCATCATGGACCTCTCCACCGGCGGCCCTGTCGACGA
GATCCGCCGCGCCATCATCGCCGAGACCAACGCCTGCATCGGAAGCGTCCCCCTCTACCAGGCGGCCCTCGACGCAGTGC
GCACCAAGAAGAAGGCCATCGTCGACATGACCGTGGACGACATCTTCGAGGGGATCATCAAGCACGCCGAGGACGGGGTC
GACTTCATCACCGTCCACTGCGGCGTGACCCGCTCCACCGTTGAGCGGATGAAGAACGAAGGGCGGCTCATGGACGTGGT
TTCACGGGGCGGCGCCTTCACCGTGGAGTGGATGGCCTACAACAACTGCGAGAACCCCCTCTTCGAGCACTTCGACCGGC
TCCTGGAGATCACGAAGGCCTACGACATGACCCTCTCCCTGGGGGACGGCTTCCGTCCCGGCTGCCTGGCCGACGCCACC
GACCGGGCCCAGATCCACGAGCTGATCATCCTGGGCGAGCTGACCCAGCGTGCCCAGGCCGCCGGCGTCCAGGTCATGAT
CGAGGGGCCGGGCCATGTCCCCCTTAACCAGATCGAGGCCAACATCCTCCTCCAGAAGCGCCTCTGCCACGGGGCACCCT
TCTATGTCCTCGGCCCCCTGGTCACCGACATCGCCCCGGGCTACGACCACATCACCTGCGCCATCGGCGGGGCCATCGCC
GCCTCGGCAGGGGCCGACTTCCTCTGCTACGTGACCCCCTCCGAGCACCTGCGCCTGCCGAGCGTCGAAGACGTCCGGGA
AGGGGTCATCGCCTCCCGCATCGCGGCCCACGCCGCCGACATCGCCAAGGGGGTCAAGGGGGCCATGGAGAAGGACATCG
CCATGGCCAAGTGCCGCAAGAAGCTCGACTGGGAGGGGCAGTTCAATCTCTCACTCGATCCGGAGAAGGCTCAGCGGCTA
CGGGCCGAATCGGGGGTTGCCGAGCACGGCGCCTGCACCATGTGCGGCGAGTTTTGCGCCTACAAGGTCATGGACGACGC
CATGGAAAAGCAGCGGGCCGCCGGCCACTGA

Upstream 100 bases:

>100_bases
GTAACGAGCCTTAGAGCGGCAGAAGGAGGGCGCTCTGAGCGGAGGCAGGTTTCCTCGCCTCACGAATTCAGCGTTCAATC
AAACCAAGGAGTATCAGCAC

Downstream 100 bases:

>100_bases
CCGGCCGATTAGCCGTTGACTTGACAGACAAAGTACGGTATTTATTTTTTCCTCATTCGCCACCGTAGCACAGGGGTAGT
GCAGCCGATTCGTAATCGGC

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase 2; HMP-P synthase 2; HMP-phosphate synthase 2; HMPP synthase 2; Thiamine biosynthesis protein thiC 2

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MATTQLEYARQGIITDKMKEAALAEGVSPEFIREGIAAGTIIICHNIKHGNGRPLAVGKGLRTKVNANIGTSADDTDITK
ELEKARVAVRHGADAIMDLSTGGPVDEIRRAIIAETNACIGSVPLYQAALDAVRTKKKAIVDMTVDDIFEGIIKHAEDGV
DFITVHCGVTRSTVERMKNEGRLMDVVSRGGAFTVEWMAYNNCENPLFEHFDRLLEITKAYDMTLSLGDGFRPGCLADAT
DRAQIHELIILGELTQRAQAAGVQVMIEGPGHVPLNQIEANILLQKRLCHGAPFYVLGPLVTDIAPGYDHITCAIGGAIA
ASAGADFLCYVTPSEHLRLPSVEDVREGVIASRIAAHAADIAKGVKGAMEKDIAMAKCRKKLDWEGQFNLSLDPEKAQRL
RAESGVAEHGACTMCGEFCAYKVMDDAMEKQRAAGH

Sequences:

>Translated_436_residues
MATTQLEYARQGIITDKMKEAALAEGVSPEFIREGIAAGTIIICHNIKHGNGRPLAVGKGLRTKVNANIGTSADDTDITK
ELEKARVAVRHGADAIMDLSTGGPVDEIRRAIIAETNACIGSVPLYQAALDAVRTKKKAIVDMTVDDIFEGIIKHAEDGV
DFITVHCGVTRSTVERMKNEGRLMDVVSRGGAFTVEWMAYNNCENPLFEHFDRLLEITKAYDMTLSLGDGFRPGCLADAT
DRAQIHELIILGELTQRAQAAGVQVMIEGPGHVPLNQIEANILLQKRLCHGAPFYVLGPLVTDIAPGYDHITCAIGGAIA
ASAGADFLCYVTPSEHLRLPSVEDVREGVIASRIAAHAADIAKGVKGAMEKDIAMAKCRKKLDWEGQFNLSLDPEKAQRL
RAESGVAEHGACTMCGEFCAYKVMDDAMEKQRAAGH
>Mature_435_residues
ATTQLEYARQGIITDKMKEAALAEGVSPEFIREGIAAGTIIICHNIKHGNGRPLAVGKGLRTKVNANIGTSADDTDITKE
LEKARVAVRHGADAIMDLSTGGPVDEIRRAIIAETNACIGSVPLYQAALDAVRTKKKAIVDMTVDDIFEGIIKHAEDGVD
FITVHCGVTRSTVERMKNEGRLMDVVSRGGAFTVEWMAYNNCENPLFEHFDRLLEITKAYDMTLSLGDGFRPGCLADATD
RAQIHELIILGELTQRAQAAGVQVMIEGPGHVPLNQIEANILLQKRLCHGAPFYVLGPLVTDIAPGYDHITCAIGGAIAA
SAGADFLCYVTPSEHLRLPSVEDVREGVIASRIAAHAADIAKGVKGAMEKDIAMAKCRKKLDWEGQFNLSLDPEKAQRLR
AESGVAEHGACTMCGEFCAYKVMDDAMEKQRAAGH

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=456, Percent_Identity=45.6140350877193, Blast_Score=375, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC2_GEOMG (Q39RJ6)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_385853.1
- STRING:   Q39RJ6
- GeneID:   3740860
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_2910
- NMPDR:   fig|269799.3.peg.3142
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   VDFMTIH
- PhylomeDB:   Q39RJ6
- ProtClustDB:   PRK13352
- BioCyc:   GMET269799:GMET_2910-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 47004; Mature: 46873

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: BINDING 68-68 BINDING 97-97 BINDING 126-126 BINDING 166-166 BINDING 268-268 BINDING 295-295

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATTQLEYARQGIITDKMKEAALAEGVSPEFIREGIAAGTIIICHNIKHGNGRPLAVGKG
CCCCHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEECCCCCCCCEEEECCC
LRTKVNANIGTSADDTDITKELEKARVAVRHGADAIMDLSTGGPVDEIRRAIIAETNACI
CCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHCCCCC
GSVPLYQAALDAVRTKKKAIVDMTVDDIFEGIIKHAEDGVDFITVHCGVTRSTVERMKNE
CCCHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHC
GRLMDVVSRGGAFTVEWMAYNNCENPLFEHFDRLLEITKAYDMTLSLGDGFRPGCLADAT
CCEEHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCC
DRAQIHELIILGELTQRAQAAGVQVMIEGPGHVPLNQIEANILLQKRLCHGAPFYVLGPL
CHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
VTDIAPGYDHITCAIGGAIAASAGADFLCYVTPSEHLRLPSVEDVREGVIASRIAAHAAD
HHHCCCCCCEEEEECCCHHHHCCCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHH
IAKGVKGAMEKDIAMAKCRKKLDWEGQFNLSLDPEKAQRLRAESGVAEHGACTMCGEFCA
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHH
YKVMDDAMEKQRAAGH
HHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ATTQLEYARQGIITDKMKEAALAEGVSPEFIREGIAAGTIIICHNIKHGNGRPLAVGKG
CCCHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEECCCCCCCCEEEECCC
LRTKVNANIGTSADDTDITKELEKARVAVRHGADAIMDLSTGGPVDEIRRAIIAETNACI
CCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHCCCCC
GSVPLYQAALDAVRTKKKAIVDMTVDDIFEGIIKHAEDGVDFITVHCGVTRSTVERMKNE
CCCHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHC
GRLMDVVSRGGAFTVEWMAYNNCENPLFEHFDRLLEITKAYDMTLSLGDGFRPGCLADAT
CCEEHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCC
DRAQIHELIILGELTQRAQAAGVQVMIEGPGHVPLNQIEANILLQKRLCHGAPFYVLGPL
CHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
VTDIAPGYDHITCAIGGAIAASAGADFLCYVTPSEHLRLPSVEDVREGVIASRIAAHAAD
HHHCCCCCCEEEEECCCHHHHCCCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHH
IAKGVKGAMEKDIAMAKCRKKLDWEGQFNLSLDPEKAQRLRAESGVAEHGACTMCGEFCA
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHH
YKVMDDAMEKQRAAGH
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA