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The map label for this gene is trmD

Identifier: 78224064

GI number: 78224064

Start: 3251020

End: 3251757

Strand: Reverse

Name: trmD

Synonym: Gmet_2868

Alternate gene names: 78224064

Gene position: 3251757-3251020 (Counterclockwise)

Preceding gene: 78224065

Following gene: 78224063

Centisome position: 81.35

GC content: 59.21

Gene sequence:

>738_bases
ATGAAATTCGATATTTTGACCCTTTTCCCTGCCATGTTCGAGGGACCGCTTACCGAAAGCATCATCCGGCGGGCAGTCGA
GAAAGGGCTCCTTGATATCCGGTTGCACCAGATCCGTGACTTTGCCACTGATCGGCACAAGGTTGTCGACGATGCACCCT
ATGGTGGTGGTGACGGGATGGTCATGAAGGTTGAGCCGATTGCCGCCTGTCTGGAAGCGGTCAAGGCCGAGCGCCCAAAG
GCGCGGGTCCTACTCACCAGCCCTCGGGGCCGTCTCTTCGATAATGCGGCGGCCCGCGAGCTGGCCCAAGAGCAGGAAGT
CATCATTATCTGCGGACGGTATGAGGGGATCGACGAGCGGGTCCGCGAGCTTTTCGTGGAGGATGAGTTTTCAATCGGCG
ATTTTGTCCTCACTGGCGGAGAGTTGGCAGCCATGGTCATGATCGACGCCACCGTGCGGTTTGTTCCCGGAGTACTCGGC
TCGCCCGGGTCGGCCGAAACTGACACGTTCTCCGACGGCCTGCTTGAGTACCCTCACTATACGCGTCCTGCCGAATTTCG
CGGCCATTCGGTCCCGGCTGTGCTGTTGTCCGGCAACCATGCAGAAGTGGCGCGTTGGCGACGTCGCAAGGCCTTGGAGG
AAACGATCCGTTCGAGACCGGATTTGCTCGAAAAGGCAGTGCTGGATAGTGACGACCGGCGCTACCTGCTAGAACTCGAA
GAGGGAGCCTCGAAGTGA

Upstream 100 bases:

>100_bases
AGAGGGAATATCTGATTCCGGCCCTTGAAGACATAGTTGTCGGTGTTGATTTGGATAAGGGAATCATGACCGTCAGCCCC
ACAGAGGGGCTGCTTGATCT

Downstream 100 bases:

>100_bases
GCGGGGCGACTCTTTCCAATGCCAATGTGAGTGTCGCGCTCCTCCACCATCCGGTTTATGACAAAAATCGGCAGGTGGTC
TCCACGGCGGTAACGAATCT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MKFDILTLFPAMFEGPLTESIIRRAVEKGLLDIRLHQIRDFATDRHKVVDDAPYGGGDGMVMKVEPIAACLEAVKAERPK
ARVLLTSPRGRLFDNAAARELAQEQEVIIICGRYEGIDERVRELFVEDEFSIGDFVLTGGELAAMVMIDATVRFVPGVLG
SPGSAETDTFSDGLLEYPHYTRPAEFRGHSVPAVLLSGNHAEVARWRRRKALEETIRSRPDLLEKAVLDSDDRRYLLELE
EGASK

Sequences:

>Translated_245_residues
MKFDILTLFPAMFEGPLTESIIRRAVEKGLLDIRLHQIRDFATDRHKVVDDAPYGGGDGMVMKVEPIAACLEAVKAERPK
ARVLLTSPRGRLFDNAAARELAQEQEVIIICGRYEGIDERVRELFVEDEFSIGDFVLTGGELAAMVMIDATVRFVPGVLG
SPGSAETDTFSDGLLEYPHYTRPAEFRGHSVPAVLLSGNHAEVARWRRRKALEETIRSRPDLLEKAVLDSDDRRYLLELE
EGASK
>Mature_245_residues
MKFDILTLFPAMFEGPLTESIIRRAVEKGLLDIRLHQIRDFATDRHKVVDDAPYGGGDGMVMKVEPIAACLEAVKAERPK
ARVLLTSPRGRLFDNAAARELAQEQEVIIICGRYEGIDERVRELFVEDEFSIGDFVLTGGELAAMVMIDATVRFVPGVLG
SPGSAETDTFSDGLLEYPHYTRPAEFRGHSVPAVLLSGNHAEVARWRRRKALEETIRSRPDLLEKAVLDSDDRRYLLELE
EGASK

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=241, Percent_Identity=50.207468879668, Blast_Score=234, Evalue=4e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_GEOMG (Q39RN8)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_385811.1
- ProteinModelPortal:   Q39RN8
- SMR:   Q39RN8
- STRING:   Q39RN8
- GeneID:   3738925
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_2868
- NMPDR:   fig|269799.3.peg.3038
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- PhylomeDB:   Q39RN8
- ProtClustDB:   PRK00026
- BioCyc:   GMET269799:GMET_2868-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 27354; Mature: 27354

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFDILTLFPAMFEGPLTESIIRRAVEKGLLDIRLHQIRDFATDRHKVVDDAPYGGGDGM
CCCCHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
VMKVEPIAACLEAVKAERPKARVLLTSPRGRLFDNAAARELAQEQEVIIICGRYEGIDER
EEEEHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHH
VRELFVEDEFSIGDFVLTGGELAAMVMIDATVRFVPGVLGSPGSAETDTFSDGLLEYPHY
HHHHHHCCCCCCCCEEEECCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
TRPAEFRGHSVPAVLLSGNHAEVARWRRRKALEETIRSRPDLLEKAVLDSDDRRYLLELE
CCCHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEEEC
EGASK
CCCCC
>Mature Secondary Structure
MKFDILTLFPAMFEGPLTESIIRRAVEKGLLDIRLHQIRDFATDRHKVVDDAPYGGGDGM
CCCCHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
VMKVEPIAACLEAVKAERPKARVLLTSPRGRLFDNAAARELAQEQEVIIICGRYEGIDER
EEEEHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHH
VRELFVEDEFSIGDFVLTGGELAAMVMIDATVRFVPGVLGSPGSAETDTFSDGLLEYPHY
HHHHHHCCCCCCCCEEEECCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
TRPAEFRGHSVPAVLLSGNHAEVARWRRRKALEETIRSRPDLLEKAVLDSDDRRYLLELE
CCCHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEEEC
EGASK
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA