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The map label for this gene is ispE

Identifier: 78224045

GI number: 78224045

Start: 3226921

End: 3227766

Strand: Reverse

Name: ispE

Synonym: Gmet_2849

Alternate gene names: 78224045

Gene position: 3227766-3226921 (Counterclockwise)

Preceding gene: 78224046

Following gene: 78224044

Centisome position: 80.75

GC content: 60.28

Gene sequence:

>846_bases
ATGAAATCCCTGACGCTCAAGGCCCCCGCCAAGGTCAACTACCGGCTCGACGTGCTCCGCCGTCGGCCTGACGGCTACCA
CGACCTCCGGATGATCATGCAGCGGATCGATCTCTGCGACGAGATTACGATTACTTTGTCCGACACTTCCGGTGTCCGGG
TCACCTGTGGCCGCGAAGGGGTACCCGACGGTCCGGAAAACATCGCTTGGCGGGCCGCCAACGCGCTTCTGGCTCTCTCC
GGGCTGGAAACGGGGATCGATATCGCCATTACCAAGAACATCCCCGTTGCTGCCGGGCTTGGCGGCGGGAGCAGCGACGC
GGCCACGGTTCTTCTGGGGGTGAACGAACTTCTGGGACTCGGCCTCTCCATCGAGCGGCTCATGGAGGTCGGCGTGACGC
TGGGTGCCGACGTGCCGTTCTTCGTCTTCGGCAAGACGGCCTTGGCCGAGGGGATTGGGGAAGTACTCACGGCAATAGAC
GCCGTCCCACCCCTCTGGCTTGTGATCGTTAACCCCAATATTCCGGTGTCGACTGCCTGGGTGTACCAAAGTTTACGGTT
GACAGGTGAGTGGAATGGCGATACTATTCCTCGCTTCTTTAAGGATGTAGCGGATGTCTGTGCCGTCCTCGCCAATGATC
TCGAATTGGTGACCATCGAGCGGTATCCGGTAATCGGTGAAATCAAGGAGCGACTGGTCGCCGCAGGGGCTGCTGGGGCA
CTTATGTCCGGCAGTGGTCCCACGGTTTTCGGACTTTTCGCTTCCGAGGAGGAGGCACGTGGCGCGGCCCAGTCCATTAC
CAATTCATCCGACTGGTTCGTAGTGGCAGTCAGGGCTTTGGTGTGA

Upstream 100 bases:

>100_bases
CTCCGACGTGCCAGTGAAGTGAAGAGATATGCCTTGATTTGTCGCGCAGTTTTGGTTTAGAAAGATACCTTTCCACCCTT
TCACAGAAAGTGATGCCCCC

Downstream 100 bases:

>100_bases
TTGGCCGTTGCCAGGCAGTACAATACCACACTGGGGTGTAGCCAAGCGGTAAGGCACCGGATTTTGATTCCGGCATTCCA
AGGTTCGAATCCTTGCACCC

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MKSLTLKAPAKVNYRLDVLRRRPDGYHDLRMIMQRIDLCDEITITLSDTSGVRVTCGREGVPDGPENIAWRAANALLALS
GLETGIDIAITKNIPVAAGLGGGSSDAATVLLGVNELLGLGLSIERLMEVGVTLGADVPFFVFGKTALAEGIGEVLTAID
AVPPLWLVIVNPNIPVSTAWVYQSLRLTGEWNGDTIPRFFKDVADVCAVLANDLELVTIERYPVIGEIKERLVAAGAAGA
LMSGSGPTVFGLFASEEEARGAAQSITNSSDWFVVAVRALV

Sequences:

>Translated_281_residues
MKSLTLKAPAKVNYRLDVLRRRPDGYHDLRMIMQRIDLCDEITITLSDTSGVRVTCGREGVPDGPENIAWRAANALLALS
GLETGIDIAITKNIPVAAGLGGGSSDAATVLLGVNELLGLGLSIERLMEVGVTLGADVPFFVFGKTALAEGIGEVLTAID
AVPPLWLVIVNPNIPVSTAWVYQSLRLTGEWNGDTIPRFFKDVADVCAVLANDLELVTIERYPVIGEIKERLVAAGAAGA
LMSGSGPTVFGLFASEEEARGAAQSITNSSDWFVVAVRALV
>Mature_281_residues
MKSLTLKAPAKVNYRLDVLRRRPDGYHDLRMIMQRIDLCDEITITLSDTSGVRVTCGREGVPDGPENIAWRAANALLALS
GLETGIDIAITKNIPVAAGLGGGSSDAATVLLGVNELLGLGLSIERLMEVGVTLGADVPFFVFGKTALAEGIGEVLTAID
AVPPLWLVIVNPNIPVSTAWVYQSLRLTGEWNGDTIPRFFKDVADVCAVLANDLELVTIERYPVIGEIKERLVAAGAAGA
LMSGSGPTVFGLFASEEEARGAAQSITNSSDWFVVAVRALV

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=272, Percent_Identity=35.2941176470588, Blast_Score=140, Evalue=7e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_GEOMG (Q39RQ7)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_385792.1
- ProteinModelPortal:   Q39RQ7
- SMR:   Q39RQ7
- STRING:   Q39RQ7
- GeneID:   3741156
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_2849
- NMPDR:   fig|269799.3.peg.3019
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   RIYNGLR
- PhylomeDB:   Q39RQ7
- ProtClustDB:   PRK14614
- BioCyc:   GMET269799:GMET_2849-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 29759; Mature: 29759

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 137-137

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLTLKAPAKVNYRLDVLRRRPDGYHDLRMIMQRIDLCDEITITLSDTSGVRVTCGREG
CCCCEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCC
VPDGPENIAWRAANALLALSGLETGIDIAITKNIPVAAGLGGGSSDAATVLLGVNELLGL
CCCCCCHHHHHHHHHHHEECCCCCCEEEEEECCCCEEEECCCCCCCHHEEEEEHHHHHHC
GLSIERLMEVGVTLGADVPFFVFGKTALAEGIGEVLTAIDAVPPLWLVIVNPNIPVSTAW
CCCHHHHHHHCHHCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHH
VYQSLRLTGEWNGDTIPRFFKDVADVCAVLANDLELVTIERYPVIGEIKERLVAAGAAGA
HHEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCC
LMSGSGPTVFGLFASEEEARGAAQSITNSSDWFVVAVRALV
EECCCCCEEEEEECCCHHHHHHHHHCCCCCCEEEEEEEHHC
>Mature Secondary Structure
MKSLTLKAPAKVNYRLDVLRRRPDGYHDLRMIMQRIDLCDEITITLSDTSGVRVTCGREG
CCCCEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCC
VPDGPENIAWRAANALLALSGLETGIDIAITKNIPVAAGLGGGSSDAATVLLGVNELLGL
CCCCCCHHHHHHHHHHHEECCCCCCEEEEEECCCCEEEECCCCCCCHHEEEEEHHHHHHC
GLSIERLMEVGVTLGADVPFFVFGKTALAEGIGEVLTAIDAVPPLWLVIVNPNIPVSTAW
CCCHHHHHHHCHHCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHH
VYQSLRLTGEWNGDTIPRFFKDVADVCAVLANDLELVTIERYPVIGEIKERLVAAGAAGA
HHEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCC
LMSGSGPTVFGLFASEEEARGAAQSITNSSDWFVVAVRALV
EECCCCCEEEEEECCCHHHHHHHHHCCCCCCEEEEEEEHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA