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The map label for this gene is rsbU [H]

Identifier: 78224014

GI number: 78224014

Start: 3186189

End: 3188138

Strand: Reverse

Name: rsbU [H]

Synonym: Gmet_2818

Alternate gene names: 78224014

Gene position: 3188138-3186189 (Counterclockwise)

Preceding gene: 78224015

Following gene: 78224011

Centisome position: 79.75

GC content: 65.08

Gene sequence:

>1950_bases
ATGGCAACTCCGCTCCGCGTCCTGATCATCGAAGATTCCGAAGACGATGCCCTTCTGATTCTCCTCGAACTCAGGAAAGG
GGGGTACGAGCCCGAGTACGTGCGGGTCGAGACCCCTGAGGCCATGATGGAGGCCATTGCCGGCCGGCAGTGGGACATCG
CCCTTTCCGACTACGTGCTCCCCCGCTTCAGCGGGATTGATGCCCTCAATATCTGGCGCCAGAGCGGTTTTGATGCCCCC
TTCATCATCGTTTCGGGGAAGATCGGCGAAGATACGGCGGTGGGGGCCATGAGGGCGGGGGCTAACGACTATTTCATTAA
GGGGAACATTTCGCGGCTCGTCCCCGCCATCCAGCGGGAGTTGAAGGAGGCGGAGCTGCGCCGGAAGAAACGGGAGGCGG
AGGAGGCGCTCCTGACCAGCGAACTCCGCTATCGCCGGCTCGTGGCGGCTGTTACCGACTACATCTATACGGTGGAGGTC
CGTGGGGGGGACGTTGTGGCAACATACCACGGTCCCGGCTGCGCGGCGGTCACCGGCTATGGCCCCGAAGAGTTCGAGGG
GGATCCGTTCCTCTGGTACCGGATGATCCACGAAGAGGACAAGCAGCGGGTGACGGAGCAGACGCAGCAGCTTCTGGAGG
GTGAAGACATCCCCTCCATGGAACACCGGATCATCCACAAGGACGGCTCCATCCGCTGGGTTCGGAACACCATCGTTCCC
CGCTATGACGAGTTAGGGGGACTCGCGGCCTACGACGGCCTCATCGCGGACATCACCGCCCGCAAGACCGCCGAAATCGA
GTTGGGCCTCCGGAGCGCCGCGCTGAATGCCGCGGCGAACGCCATTGTCATCAGCGATCCCGGCGGGCGGGTCATCTGGG
TCAATCCCGCCTTCACGAATCTTACCGGCTACACCCTGGAGGAAATCGCCGGCCGCGGTCTCGGCATCCTCAAGTCGGGG
GTCCACGGCGAGGAGTTTTACCGGGATCTCTGGGAGACGATCCGGGCCGGAAAGGTCTGGCGCGGCGAGATGATCAACCG
CCGCAAGGACGGTTCCCTCTACCCCGAGGCCCAGACCATTACCCCCGTGGTGGATGAGGCGGGAGTGGTTCGCCACTATG
TCGCCATCAAGGAGGACGTGACCGAGCGCAAGCGTGCCCAGGAGGCACTCCTGGAGAATGCCCGCATGTCCTGCGACATG
GAGCTGGCGCGGCAGATCCAGGGGGCGCTCCTGCCGTCGGCCCCTCCGGTCGTCGCGGGAGTCCGTTGCGCCGGGCGGTG
GGTTCCCGCGGCCCATGTGGGGGGAGACTACTATGACTTCTTCCCCCGGGACGACGGTTCCTTCGACACGGTTATCGCCG
ACGTCTCGGGCCATAGTGTCGGCGCGGCCCTCATCATGACCGAGGCCCGCAGCGTTATCCGCACCCAGGCCCGCTCCGGC
GGTTCCCCTGCCGCCATCGTCAGATCCCTCAATGAGAACCTCTACGAAGACCTCACCCGGGCGGAGCTCTTCACCACCAT
GTTCTACGCCTCCTACCGGCCGACATCGGGCATCCTCACCTACGCCAGCGCGGGGCACAACCCGCCGCTCCTCTACCGGC
GGGCCGAGGGGAGGTGTCTGGAACTGGACGCGGATGGGCTCATCCTCGGGGTAAAACCCCAGGTGACGTTCGAGGAGCGG
GAGGTGCGGCTGGCGCCGGGAGATATCCTGCTCCTCTACACCGACGGGATCACCGAGTCCGATAACGGCAGCGGAGAGCT
TTTCGGGGTCGGCCGCCTCAGTGCCCTCCTGGCGTCGCTCCACGGCGAGGAGCCCGAGGCGGTCATCGAGGCCGTGCTGT
CGGAACTGGCCGCATTCACCGGCGGCAGGCCCCCTGCGGACGACATCTGCACGGTGGCGGTGAAAATCACGCCATCATCC
GGCTCAGGATCAGGAGAAAGTTGCGGGTGA

Upstream 100 bases:

>100_bases
CGAGCGGGTTCCCGCGTAGGCCCGTCCTTTCCCGTTGACGCTTGTCCGCGGCTCCTCCCGTAGTACAATGAACATGACAT
TTTTCCCATCGAGGAACCCT

Downstream 100 bases:

>100_bases
GGAGAGGATGGAACTCCCGCCCCCGCCGGTCAAGGAGCATGCCGGCGATCTGGCGCAGTTCCACGGAGTCGCTGTAGGCC
CGGCGGGTACGAAGCGCGTC

Product: putative PAS/PAC sensor protein

Products: NA

Alternate protein names: Sigma factor sigB regulation protein rsbU [H]

Number of amino acids: Translated: 649; Mature: 648

Protein sequence:

>649_residues
MATPLRVLIIEDSEDDALLILLELRKGGYEPEYVRVETPEAMMEAIAGRQWDIALSDYVLPRFSGIDALNIWRQSGFDAP
FIIVSGKIGEDTAVGAMRAGANDYFIKGNISRLVPAIQRELKEAELRRKKREAEEALLTSELRYRRLVAAVTDYIYTVEV
RGGDVVATYHGPGCAAVTGYGPEEFEGDPFLWYRMIHEEDKQRVTEQTQQLLEGEDIPSMEHRIIHKDGSIRWVRNTIVP
RYDELGGLAAYDGLIADITARKTAEIELGLRSAALNAAANAIVISDPGGRVIWVNPAFTNLTGYTLEEIAGRGLGILKSG
VHGEEFYRDLWETIRAGKVWRGEMINRRKDGSLYPEAQTITPVVDEAGVVRHYVAIKEDVTERKRAQEALLENARMSCDM
ELARQIQGALLPSAPPVVAGVRCAGRWVPAAHVGGDYYDFFPRDDGSFDTVIADVSGHSVGAALIMTEARSVIRTQARSG
GSPAAIVRSLNENLYEDLTRAELFTTMFYASYRPTSGILTYASAGHNPPLLYRRAEGRCLELDADGLILGVKPQVTFEER
EVRLAPGDILLLYTDGITESDNGSGELFGVGRLSALLASLHGEEPEAVIEAVLSELAAFTGGRPPADDICTVAVKITPSS
GSGSGESCG

Sequences:

>Translated_649_residues
MATPLRVLIIEDSEDDALLILLELRKGGYEPEYVRVETPEAMMEAIAGRQWDIALSDYVLPRFSGIDALNIWRQSGFDAP
FIIVSGKIGEDTAVGAMRAGANDYFIKGNISRLVPAIQRELKEAELRRKKREAEEALLTSELRYRRLVAAVTDYIYTVEV
RGGDVVATYHGPGCAAVTGYGPEEFEGDPFLWYRMIHEEDKQRVTEQTQQLLEGEDIPSMEHRIIHKDGSIRWVRNTIVP
RYDELGGLAAYDGLIADITARKTAEIELGLRSAALNAAANAIVISDPGGRVIWVNPAFTNLTGYTLEEIAGRGLGILKSG
VHGEEFYRDLWETIRAGKVWRGEMINRRKDGSLYPEAQTITPVVDEAGVVRHYVAIKEDVTERKRAQEALLENARMSCDM
ELARQIQGALLPSAPPVVAGVRCAGRWVPAAHVGGDYYDFFPRDDGSFDTVIADVSGHSVGAALIMTEARSVIRTQARSG
GSPAAIVRSLNENLYEDLTRAELFTTMFYASYRPTSGILTYASAGHNPPLLYRRAEGRCLELDADGLILGVKPQVTFEER
EVRLAPGDILLLYTDGITESDNGSGELFGVGRLSALLASLHGEEPEAVIEAVLSELAAFTGGRPPADDICTVAVKITPSS
GSGSGESCG
>Mature_648_residues
ATPLRVLIIEDSEDDALLILLELRKGGYEPEYVRVETPEAMMEAIAGRQWDIALSDYVLPRFSGIDALNIWRQSGFDAPF
IIVSGKIGEDTAVGAMRAGANDYFIKGNISRLVPAIQRELKEAELRRKKREAEEALLTSELRYRRLVAAVTDYIYTVEVR
GGDVVATYHGPGCAAVTGYGPEEFEGDPFLWYRMIHEEDKQRVTEQTQQLLEGEDIPSMEHRIIHKDGSIRWVRNTIVPR
YDELGGLAAYDGLIADITARKTAEIELGLRSAALNAAANAIVISDPGGRVIWVNPAFTNLTGYTLEEIAGRGLGILKSGV
HGEEFYRDLWETIRAGKVWRGEMINRRKDGSLYPEAQTITPVVDEAGVVRHYVAIKEDVTERKRAQEALLENARMSCDME
LARQIQGALLPSAPPVVAGVRCAGRWVPAAHVGGDYYDFFPRDDGSFDTVIADVSGHSVGAALIMTEARSVIRTQARSGG
SPAAIVRSLNENLYEDLTRAELFTTMFYASYRPTSGILTYASAGHNPPLLYRRAEGRCLELDADGLILGVKPQVTFEERE
VRLAPGDILLLYTDGITESDNGSGELFGVGRLSALLASLHGEEPEAVIEAVLSELAAFTGGRPPADDICTVAVKITPSSG
SGSGESCG

Specific function: Positive regulator of sigma-B activity. Dephosphorylates rsbV in response to environmental stress conveyed from the rsbXST module [H]

COG id: COG2208

COG function: function code TK; Serine phosphatase RsbU, regulator of sigma subunit

Gene ontology:

Cell location: Integral Membrane. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PP2C-like domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017944
- InterPro:   IPR001932
- InterPro:   IPR014787
- InterPro:   IPR010822
- ProDom:   PD685490 [H]

Pfam domain/function: PF08673 RsbU_N; PF07228 SpoIIE [H]

EC number: =3.1.3.3 [H]

Molecular weight: Translated: 70937; Mature: 70806

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATPLRVLIIEDSEDDALLILLELRKGGYEPEYVRVETPEAMMEAIAGRQWDIALSDYVL
CCCCEEEEEEECCCCCEEEEEEEECCCCCCCCEEEEECHHHHHHHHCCCCEEEEHHHHHC
PRFSGIDALNIWRQSGFDAPFIIVSGKIGEDTAVGAMRAGANDYFIKGNISRLVPAIQRE
CCCCCCCHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHCCCCCEEEECCHHHHHHHHHHH
LKEAELRRKKREAEEALLTSELRYRRLVAAVTDYIYTVEVRGGDVVATYHGPGCAAVTGY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCEEEEEEECCCEEEEECC
GPEEFEGDPFLWYRMIHEEDKQRVTEQTQQLLEGEDIPSMEHRIIHKDGSIRWVRNTIVP
CHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEECCCCEEEEECCCCC
RYDELGGLAAYDGLIADITARKTAEIELGLRSAALNAAANAIVISDPGGRVIWVNPAFTN
CHHHHCCCHHHCCHHEEHHCCCEEEEEECHHHHHHHHCCCEEEEECCCCEEEEECCCCCC
LTGYTLEEIAGRGLGILKSGVHGEEFYRDLWETIRAGKVWRGEMINRRKDGSLYPEAQTI
CCCCCHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCEEHHHHCCCCCCCCCCCCCCC
TPVVDEAGVVRHYVAIKEDVTERKRAQEALLENARMSCDMELARQIQGALLPSAPPVVAG
CHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHH
VRCAGRWVPAAHVGGDYYDFFPRDDGSFDTVIADVSGHSVGAALIMTEARSVIRTQARSG
HECCCCCCCHHHCCCCEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCC
GSPAAIVRSLNENLYEDLTRAELFTTMFYASYRPTSGILTYASAGHNPPLLYRRAEGRCL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEECCCCEE
ELDADGLILGVKPQVTFEEREVRLAPGDILLLYTDGITESDNGSGELFGVGRLSALLASL
EECCCCEEEECCCCEEECCCEEEECCCCEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHH
HGEEPEAVIEAVLSELAAFTGGRPPADDICTVAVKITPSSGSGSGESCG
CCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
ATPLRVLIIEDSEDDALLILLELRKGGYEPEYVRVETPEAMMEAIAGRQWDIALSDYVL
CCCEEEEEEECCCCCEEEEEEEECCCCCCCCEEEEECHHHHHHHHCCCCEEEEHHHHHC
PRFSGIDALNIWRQSGFDAPFIIVSGKIGEDTAVGAMRAGANDYFIKGNISRLVPAIQRE
CCCCCCCHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHCCCCCEEEECCHHHHHHHHHHH
LKEAELRRKKREAEEALLTSELRYRRLVAAVTDYIYTVEVRGGDVVATYHGPGCAAVTGY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCEEEEEEECCCEEEEECC
GPEEFEGDPFLWYRMIHEEDKQRVTEQTQQLLEGEDIPSMEHRIIHKDGSIRWVRNTIVP
CHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEECCCCEEEEECCCCC
RYDELGGLAAYDGLIADITARKTAEIELGLRSAALNAAANAIVISDPGGRVIWVNPAFTN
CHHHHCCCHHHCCHHEEHHCCCEEEEEECHHHHHHHHCCCEEEEECCCCEEEEECCCCCC
LTGYTLEEIAGRGLGILKSGVHGEEFYRDLWETIRAGKVWRGEMINRRKDGSLYPEAQTI
CCCCCHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCEEHHHHCCCCCCCCCCCCCCC
TPVVDEAGVVRHYVAIKEDVTERKRAQEALLENARMSCDMELARQIQGALLPSAPPVVAG
CHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHH
VRCAGRWVPAAHVGGDYYDFFPRDDGSFDTVIADVSGHSVGAALIMTEARSVIRTQARSG
HECCCCCCCHHHCCCCEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCC
GSPAAIVRSLNENLYEDLTRAELFTTMFYASYRPTSGILTYASAGHNPPLLYRRAEGRCL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEECCCCEE
ELDADGLILGVKPQVTFEEREVRLAPGDILLLYTDGITESDNGSGELFGVGRLSALLASL
EECCCCEEEECCCCEEECCCEEEECCCCEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHH
HGEEPEAVIEAVLSELAAFTGGRPPADDICTVAVKITPSSGSGSGESCG
CCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8002610; 8002609; 9384377; 8824586; 9786195; 12499568 [H]