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The map label for this gene is barA [H]

Identifier: 78223984

GI number: 78223984

Start: 3151742

End: 3153472

Strand: Reverse

Name: barA [H]

Synonym: Gmet_2788

Alternate gene names: 78223984

Gene position: 3153472-3151742 (Counterclockwise)

Preceding gene: 78223987

Following gene: 78223982

Centisome position: 78.89

GC content: 60.02

Gene sequence:

>1731_bases
ATGATGACGAAAAGCGGCATGGCATGTCGAAGCTGTTTCCTGGTGCTCCTCATCGCGCTGCTCACTTTTTCCAACCTCGC
AATCCCCACTGCCGGAGCCGAGGCCCGGGTCGTGACCGTCGGTGTCTATGAAAACTCCCCCAAGATCTTCACCGACGACT
CCGGCAAACCGTCGGGCATCTTCATCGATATTATCGAGCACATCGCGAAAACCGAGGGGTGGAACCTGCACTATGTGCAC
GGCACCTGGGTGGAAGGCCTGGATCGGCTCGCGAAAGGGGAGATCGACCTCATGCCGGACGTGGCCTATACGGCCGAGCG
GGAAAACCTCTACTCCTTCCACAGGATTCCGGTTCTCACCGGCTGGTCCCAGGTGTATGCCCGCAAGGGGAGCGGCATCC
AATCGATCCTGAACCTGAACGGAAAACGGGTTGCCGCCCTGGAGCAGACGATCCAGTTTGAAACCTTCAGGAGGCTCGCC
AGCAGCTTCGGGTTGAAGATCACCCTCATCCCCGTGCCCGATTACAAGACCGAGTTCGACATGCTTGCCAATGGCAAGAT
TGATGCCGCCGTCACCAACCGTTTCTACGGGTTGAGGCACGCGAGAGAGTCGGGGCTGGAAGATACGCCGATCATGTTCG
ACCCCGCCCCCTTCTTTTTTGCCGCGCCCCGGGATGCTTCCCGTCACCTGCTGGACGCCATCGACCGTCACCTCGCGGAG
CTGAAAAAGGACCCCCGGTCGGCCTATTATGCATCGCTGAACCGCTGGACCTCGGAAGACGTGCAGTTCAAACTGCCTGT
CTGGCTGCAAGTTTCGGGCCTTATCCTGGGGGTGGTTGTCATAATGACGGTGACCGGGTTCATCCTCTGGAACCGCACGC
TCCGGCGGACCGTCAAGCTCCGCACGGCGCAGCTGGAGCAGGAACTGGCCGAGCGCAAAATGGCCGAAGAGGAGCTTCGG
AAGTACCGGGAAAATCTGGAGGATCTCGTCAGGGAGCGAACTGCCGAGCTCGCGGTGGCCAAGGAGCGGGCCGAGGCGGC
CGACCAGCTGAAATCCGCCTTTCTCGCCACCATGTCCCATGAACTGCGGACCCCCCTCAACTCCATCATCGGCTTTACCG
GCATCCTGCTGCAGGGGCTGGGCGGCCCCATCAATGACGAACAGGCCAAGCAGCTGACCATGGTCAGGCATAGCGCCAAC
CACCTGCTTTCCCTGATCAGCGACGTTCTCGATATCTCCAAGATCGAGGCGGGGCAGTTGAAGATATCTCCTGAGCCGTT
CCAACTGCGGGAGTCCGTTCAGAAGGTCACCCAATCGGTGGCCGGCCTCGTTGACAAAAAAGGTCTGCGCCTCATCCTGG
AAATCGCGGATGATGTGGGAAGCATCGTCAGCGACCAGCGGCGCGTGGAACAGGTGCTGCTGAATCTACTCAGCAACGCG
GTCAAGTTCACCGAACAGGGGACCATCAGCGTGCGTTGCTCCCGCGAATCGGAGTGGTACGTCACCAGCGTCGCCGATTC
CGGCATCGGCATCAAGGAAGAGGATCTGGAACGCCTCTTCAAGCCGTTCCATCAGGTTGACACCGGCTTGAGCCGAAAAT
ACGAAGGAACGGGATTGGGGCTCTCCATCTGCAAGCGGCTGGTCGAGTTGATGGGGGGGAGCATTACGGTGGAAAGCCGC
CAGGGAGAAGGGAGCACGTTCTGTTTTCTGCTGCCGGCCAACGGGGGGTAG

Upstream 100 bases:

>100_bases
TTTGTTGTTCACGATACAGTCCTTTTGGCTTGATCTAGTGCGTACTGTATCTTACCGTATGTCGGTTTTTTGGCAACAAT
TAATGAGCGGAGTGCATGGT

Downstream 100 bases:

>100_bases
TTCTCCCGCGCACTCACCCCCGCAGGTTGTGCCAGAGGTTCACCATGAAGTCCTGGGCGTTGGTCAGGATGGCCCGGGCC
TGGGCGGAGCCCCGGTTGGC

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 576; Mature: 576

Protein sequence:

>576_residues
MMTKSGMACRSCFLVLLIALLTFSNLAIPTAGAEARVVTVGVYENSPKIFTDDSGKPSGIFIDIIEHIAKTEGWNLHYVH
GTWVEGLDRLAKGEIDLMPDVAYTAERENLYSFHRIPVLTGWSQVYARKGSGIQSILNLNGKRVAALEQTIQFETFRRLA
SSFGLKITLIPVPDYKTEFDMLANGKIDAAVTNRFYGLRHARESGLEDTPIMFDPAPFFFAAPRDASRHLLDAIDRHLAE
LKKDPRSAYYASLNRWTSEDVQFKLPVWLQVSGLILGVVVIMTVTGFILWNRTLRRTVKLRTAQLEQELAERKMAEEELR
KYRENLEDLVRERTAELAVAKERAEAADQLKSAFLATMSHELRTPLNSIIGFTGILLQGLGGPINDEQAKQLTMVRHSAN
HLLSLISDVLDISKIEAGQLKISPEPFQLRESVQKVTQSVAGLVDKKGLRLILEIADDVGSIVSDQRRVEQVLLNLLSNA
VKFTEQGTISVRCSRESEWYVTSVADSGIGIKEEDLERLFKPFHQVDTGLSRKYEGTGLGLSICKRLVELMGGSITVESR
QGEGSTFCFLLPANGG

Sequences:

>Translated_576_residues
MMTKSGMACRSCFLVLLIALLTFSNLAIPTAGAEARVVTVGVYENSPKIFTDDSGKPSGIFIDIIEHIAKTEGWNLHYVH
GTWVEGLDRLAKGEIDLMPDVAYTAERENLYSFHRIPVLTGWSQVYARKGSGIQSILNLNGKRVAALEQTIQFETFRRLA
SSFGLKITLIPVPDYKTEFDMLANGKIDAAVTNRFYGLRHARESGLEDTPIMFDPAPFFFAAPRDASRHLLDAIDRHLAE
LKKDPRSAYYASLNRWTSEDVQFKLPVWLQVSGLILGVVVIMTVTGFILWNRTLRRTVKLRTAQLEQELAERKMAEEELR
KYRENLEDLVRERTAELAVAKERAEAADQLKSAFLATMSHELRTPLNSIIGFTGILLQGLGGPINDEQAKQLTMVRHSAN
HLLSLISDVLDISKIEAGQLKISPEPFQLRESVQKVTQSVAGLVDKKGLRLILEIADDVGSIVSDQRRVEQVLLNLLSNA
VKFTEQGTISVRCSRESEWYVTSVADSGIGIKEEDLERLFKPFHQVDTGLSRKYEGTGLGLSICKRLVELMGGSITVESR
QGEGSTFCFLLPANGG
>Mature_576_residues
MMTKSGMACRSCFLVLLIALLTFSNLAIPTAGAEARVVTVGVYENSPKIFTDDSGKPSGIFIDIIEHIAKTEGWNLHYVH
GTWVEGLDRLAKGEIDLMPDVAYTAERENLYSFHRIPVLTGWSQVYARKGSGIQSILNLNGKRVAALEQTIQFETFRRLA
SSFGLKITLIPVPDYKTEFDMLANGKIDAAVTNRFYGLRHARESGLEDTPIMFDPAPFFFAAPRDASRHLLDAIDRHLAE
LKKDPRSAYYASLNRWTSEDVQFKLPVWLQVSGLILGVVVIMTVTGFILWNRTLRRTVKLRTAQLEQELAERKMAEEELR
KYRENLEDLVRERTAELAVAKERAEAADQLKSAFLATMSHELRTPLNSIIGFTGILLQGLGGPINDEQAKQLTMVRHSAN
HLLSLISDVLDISKIEAGQLKISPEPFQLRESVQKVTQSVAGLVDKKGLRLILEIADDVGSIVSDQRRVEQVLLNLLSNA
VKFTEQGTISVRCSRESEWYVTSVADSGIGIKEEDLERLFKPFHQVDTGLSRKYEGTGLGLSICKRLVELMGGSITVESR
QGEGSTFCFLLPANGG

Specific function: Member of the two-component regulatory system UvrY/BarA involved in the regulation of carbon metabolism via the CsrA/CsrB regulatory system. Phosphorylates UvrY, probably via a four-step phosphorelay [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=253, Percent_Identity=44.2687747035573, Blast_Score=195, Evalue=6e-51,
Organism=Escherichia coli, GI145693157, Length=248, Percent_Identity=43.9516129032258, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI87081816, Length=264, Percent_Identity=41.6666666666667, Blast_Score=181, Evalue=2e-46,
Organism=Escherichia coli, GI48994928, Length=232, Percent_Identity=38.3620689655172, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1788713, Length=248, Percent_Identity=35.4838709677419, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1790436, Length=220, Percent_Identity=32.7272727272727, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1786600, Length=245, Percent_Identity=33.0612244897959, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1786783, Length=237, Percent_Identity=32.0675105485232, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786912, Length=245, Percent_Identity=31.0204081632653, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788549, Length=242, Percent_Identity=28.9256198347107, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1788393, Length=229, Percent_Identity=25.3275109170306, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87082128, Length=234, Percent_Identity=27.3504273504274, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1790346, Length=237, Percent_Identity=26.1603375527426, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1790861, Length=306, Percent_Identity=23.5294117647059, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1790551, Length=311, Percent_Identity=27.3311897106109, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787894, Length=222, Percent_Identity=24.3243243243243, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1788279, Length=207, Percent_Identity=26.5700483091787, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1790300, Length=250, Percent_Identity=28, Blast_Score=63, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=91, Percent_Identity=43.956043956044, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR019247
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF09984 DUF2222; PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 64144; Mature: 64144

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTKSGMACRSCFLVLLIALLTFSNLAIPTAGAEARVVTVGVYENSPKIFTDDSGKPSGI
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCEEEECCCCCCCCC
FIDIIEHIAKTEGWNLHYVHGTWVEGLDRLAKGEIDLMPDVAYTAERENLYSFHRIPVLT
HHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHCCCEEE
GWSQVYARKGSGIQSILNLNGKRVAALEQTIQFETFRRLASSFGLKITLIPVPDYKTEFD
CHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHH
MLANGKIDAAVTNRFYGLRHARESGLEDTPIMFDPAPFFFAAPRDASRHLLDAIDRHLAE
HHCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEECCCCHHHHHHHHHHHHHHH
LKKDPRSAYYASLNRWTSEDVQFKLPVWLQVSGLILGVVVIMTVTGFILWNRTLRRTVKL
HHCCHHHHHHHHHHCCCCCCCEEECCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTAQLEQELAERKMAEEELRKYRENLEDLVRERTAELAVAKERAEAADQLKSAFLATMSH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELRTPLNSIIGFTGILLQGLGGPINDEQAKQLTMVRHSANHLLSLISDVLDISKIEAGQL
HHHCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
KISPEPFQLRESVQKVTQSVAGLVDKKGLRLILEIADDVGSIVSDQRRVEQVLLNLLSNA
EECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKFTEQGTISVRCSRESEWYVTSVADSGIGIKEEDLERLFKPFHQVDTGLSRKYEGTGLG
HHHCCCCEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
LSICKRLVELMGGSITVESRQGEGSTFCFLLPANGG
HHHHHHHHHHHCCEEEEECCCCCCCEEEEEEECCCC
>Mature Secondary Structure
MMTKSGMACRSCFLVLLIALLTFSNLAIPTAGAEARVVTVGVYENSPKIFTDDSGKPSGI
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCEEEECCCCCCCCC
FIDIIEHIAKTEGWNLHYVHGTWVEGLDRLAKGEIDLMPDVAYTAERENLYSFHRIPVLT
HHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHCCCEEE
GWSQVYARKGSGIQSILNLNGKRVAALEQTIQFETFRRLASSFGLKITLIPVPDYKTEFD
CHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHH
MLANGKIDAAVTNRFYGLRHARESGLEDTPIMFDPAPFFFAAPRDASRHLLDAIDRHLAE
HHCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEECCCCHHHHHHHHHHHHHHH
LKKDPRSAYYASLNRWTSEDVQFKLPVWLQVSGLILGVVVIMTVTGFILWNRTLRRTVKL
HHCCHHHHHHHHHHCCCCCCCEEECCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTAQLEQELAERKMAEEELRKYRENLEDLVRERTAELAVAKERAEAADQLKSAFLATMSH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELRTPLNSIIGFTGILLQGLGGPINDEQAKQLTMVRHSANHLLSLISDVLDISKIEAGQL
HHHCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
KISPEPFQLRESVQKVTQSVAGLVDKKGLRLILEIADDVGSIVSDQRRVEQVLLNLLSNA
EECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKFTEQGTISVRCSRESEWYVTSVADSGIGIKEEDLERLFKPFHQVDTGLSRKYEGTGLG
HHHCCCCEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
LSICKRLVELMGGSITVESRQGEGSTFCFLLPANGG
HHHHHHHHHHHCCEEEEECCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]