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The map label for this gene is nifA [H]

Identifier: 78223876

GI number: 78223876

Start: 3028043

End: 3028780

Strand: Reverse

Name: nifA [H]

Synonym: Gmet_2680

Alternate gene names: 78223876

Gene position: 3028780-3028043 (Counterclockwise)

Preceding gene: 78223877

Following gene: 78223875

Centisome position: 75.77

GC content: 54.61

Gene sequence:

>738_bases
ATGCATATGGTACGCTGCAGAAAGTGCGGAAGAGTAATGAAGAGTGAACGGATCGATCCCTACAGGGTGAAGCTGTCCTG
TGTCTGCGGCTTTTCCGATTTCCGGGCCGTTCCCGACAAGGCCAAAACGGTGAATCCCTTCTACCAGAAGGCGAGTTTGA
CCCCCTTTGTCGAGAGTGAAAAGGGGAAGATGGTTCTGACCATGCAGCGGGCCAACCGGGAACATCTGGAGATTATCTCC
CTGGAAGAGATCAGCATGCTGGTCTCCTCCGACTTCAACCTGCCGGAAGTTCTCCAACACGTGACGAAAAAGGTGGCCGG
GCAGCTTAAGGTGAGCGTCTGCAGCATCTACCTCCGGGAGGGGGACGACCTCGTACTGGCCGCTACCCATGGCTTCGACC
CCGCCTTCATTGGCAAGATCCGGCTCAGGATCGGCGAGGGGATCACCGGCACCGTCGCGAAGGAGGGGAGCCATATATCC
CTTACCCATGCCTCTCGCGATTCACGTTACAAGCATTTCTCCGAGCTCCAGGAAGAAAAATACAACTCCATGCTCTCCTT
TCCCATCAGCGACAAGAAGGAGGTGTATGGAGTCATCAATCTCAATACCACCTCCATCAAGTCGTTCCAGGAGGATGAGA
TCTACTTTGTCTCCATTATTGCCAACCTGATCCTCACAGCCATTAAGCTTCGGCAGCAGGTGGCCACGGGCAAAAAAGCG
CTGCAACCGTCTGCTTGA

Upstream 100 bases:

>100_bases
CAAAACCGCCCATGTGACACTTCCGGCAGAATATACTGCGGAGCAGTAGTCTCGGCAAAACTACGGCACACTGGAGATTA
TTTCAAGGAGAGGGTTTTTC

Downstream 100 bases:

>100_bases
CAGGGGGGGACACCATGCTAGACTCAGATTCATCGTACCACGCGCCAGAAGCGGAGTTTATCATTGGCAGAGCAGGCAAA
AAAACGGATACTGGTTGTTG

Product: putative GAF sensor protein

Products: pyruvate; proteinN-pros-phosphohistidine [C]

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MHMVRCRKCGRVMKSERIDPYRVKLSCVCGFSDFRAVPDKAKTVNPFYQKASLTPFVESEKGKMVLTMQRANREHLEIIS
LEEISMLVSSDFNLPEVLQHVTKKVAGQLKVSVCSIYLREGDDLVLAATHGFDPAFIGKIRLRIGEGITGTVAKEGSHIS
LTHASRDSRYKHFSELQEEKYNSMLSFPISDKKEVYGVINLNTTSIKSFQEDEIYFVSIIANLILTAIKLRQQVATGKKA
LQPSA

Sequences:

>Translated_245_residues
MHMVRCRKCGRVMKSERIDPYRVKLSCVCGFSDFRAVPDKAKTVNPFYQKASLTPFVESEKGKMVLTMQRANREHLEIIS
LEEISMLVSSDFNLPEVLQHVTKKVAGQLKVSVCSIYLREGDDLVLAATHGFDPAFIGKIRLRIGEGITGTVAKEGSHIS
LTHASRDSRYKHFSELQEEKYNSMLSFPISDKKEVYGVINLNTTSIKSFQEDEIYFVSIIANLILTAIKLRQQVATGKKA
LQPSA
>Mature_245_residues
MHMVRCRKCGRVMKSERIDPYRVKLSCVCGFSDFRAVPDKAKTVNPFYQKASLTPFVESEKGKMVLTMQRANREHLEIIS
LEEISMLVSSDFNLPEVLQHVTKKVAGQLKVSVCSIYLREGDDLVLAATHGFDPAFIGKIRLRIGEGITGTVAKEGSHIS
LTHASRDSRYKHFSELQEEKYNSMLSFPISDKKEVYGVINLNTTSIKSFQEDEIYFVSIIANLILTAIKLRQQVATGKKA
LQPSA

Specific function: Required for activation of most nif operons, which are directly involved in nitrogen fixation [H]

COG id: COG3605

COG function: function code T; Signal transduction protein containing GAF and PtsI domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789193, Length=160, Percent_Identity=25.625, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR010113
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: 2.7.3.9 [C]

Molecular weight: Translated: 27546; Mature: 27546

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHMVRCRKCGRVMKSERIDPYRVKLSCVCGFSDFRAVPDKAKTVNPFYQKASLTPFVESE
CCCHHHHHHHHHHHHCCCCCEEEEEEEEECCHHHHCCCCCCCCCCHHHHHCCCCCCEECC
KGKMVLTMQRANREHLEIISLEEISMLVSSDFNLPEVLQHVTKKVAGQLKVSVCSIYLRE
CCCEEEEEECCCCCCEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEEEEEEEEC
GDDLVLAATHGFDPAFIGKIRLRIGEGITGTVAKEGSHISLTHASRDSRYKHFSELQEEK
CCCEEEEEECCCCHHHEEEEEEEECCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHHHH
YNSMLSFPISDKKEVYGVINLNTTSIKSFQEDEIYFVSIIANLILTAIKLRQQVATGKKA
HHHEEECCCCCCCCEEEEEECCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHH
LQPSA
CCCCC
>Mature Secondary Structure
MHMVRCRKCGRVMKSERIDPYRVKLSCVCGFSDFRAVPDKAKTVNPFYQKASLTPFVESE
CCCHHHHHHHHHHHHCCCCCEEEEEEEEECCHHHHCCCCCCCCCCHHHHHCCCCCCEECC
KGKMVLTMQRANREHLEIISLEEISMLVSSDFNLPEVLQHVTKKVAGQLKVSVCSIYLRE
CCCEEEEEECCCCCCEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEEEEEEEEC
GDDLVLAATHGFDPAFIGKIRLRIGEGITGTVAKEGSHISLTHASRDSRYKHFSELQEEK
CCCEEEEEECCCCHHHEEEEEEEECCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHHHH
YNSMLSFPISDKKEVYGVINLNTTSIKSFQEDEIYFVSIIANLILTAIKLRQQVATGKKA
HHHEEECCCCCCCCEEEEEECCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHH
LQPSA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0105 {histidine-containing} 0.195 {phosphoenolpyruvate}} [C]

Substrates: Phosphoenolpyruvate; protein histidine [C]

Specific reaction: Phosphoenolpyruvate + protein histidine = pyruvate + proteinN-pros-phosphohistidine [C]

General reaction: Phospho group transfer [C]

Inhibitor: 2-Hydroxy -3-butenoic acid [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1779763; 1362170 [H]