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The map label for this gene is ptsN [H]

Identifier: 78223801

GI number: 78223801

Start: 2953773

End: 2954459

Strand: Direct

Name: ptsN [H]

Synonym: Gmet_2604

Alternate gene names: 78223801

Gene position: 2953773-2954459 (Clockwise)

Preceding gene: 78223800

Following gene: 78223803

Centisome position: 73.89

GC content: 60.55

Gene sequence:

>687_bases
ATGCTGCTTGAACCATCCGAAGCCGCCCAGGCCCTGAATGTCGATGAAAAAACCATAGTCCGCTGGATCAGGAAGGACAA
CCTGCCCGCTGAATCCATGCACGGCGATTACCGGATCAATCCGGTGGACTTGCTGGAATGGGCAACGGAACGGGGCATCA
AGGTCAATCCGGCCCTCTACAAGATGCACGATGCGGATACCCGTCCGCTGCCGACCCTGTGTCAGGCGCTCCAGGCCGGC
GGCATCCATTGCGGCTTCCCGGGCAATGACAAGGAAACCGTATTGCGCAACGTTGTTGCCGCTCTGGAGTTGCCGCCCCA
GATCGATCCCGAGTTCATACTGCAGGTGCTGCTGGCCCGGGAGGCCATGGGCACCACGGCGGTTGGTGACGGCATCGCCA
TCCCCCATGTGCGCAACCCCATCCTGGTGCAGCTGCCGGTCCCGAAGATTTCCCTGAGCTTTCTCGCACAGCCGGTTGAT
TTCGGCGCCCTGGACGGCAAGCCGGTGCAGATACTGTTCACCATCATCTCGCCGACCATCAGGATGCATCTGCACCTGCT
CTCGAGGCTGGCCTATTGCCTGCGGGACCAACGCCTGCGCGACATCCTCGGTAAGCAATGCAACGCCAGGGCGATCATGG
ACGTAATCCTCGAGATCGAGAAAGACATCGGAAGCGCTCCCCTATGA

Upstream 100 bases:

>100_bases
TCGGCTTCATCAACAAAGCCCTCATGTGTCAGGTGAATAAGGCGCTCTGTTTACAGCTCGGCATCGAGGAGCCGCCGGGA
CACCTCAAAGGATAACGTCA

Downstream 100 bases:

>100_bases
CCGAAAACGTCCTGCCCCCTCTCGGGCACCGGGCCGGTAAGTCAGGCGCAAAAAAAGCCGCATCCGAAGATGCGGCTTTT
TTTGCGCCAGGAAAAGTACT

Product: putative PTS IIA-like nitrogen-regulatory protein PtsN

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MLLEPSEAAQALNVDEKTIVRWIRKDNLPAESMHGDYRINPVDLLEWATERGIKVNPALYKMHDADTRPLPTLCQALQAG
GIHCGFPGNDKETVLRNVVAALELPPQIDPEFILQVLLAREAMGTTAVGDGIAIPHVRNPILVQLPVPKISLSFLAQPVD
FGALDGKPVQILFTIISPTIRMHLHLLSRLAYCLRDQRLRDILGKQCNARAIMDVILEIEKDIGSAPL

Sequences:

>Translated_228_residues
MLLEPSEAAQALNVDEKTIVRWIRKDNLPAESMHGDYRINPVDLLEWATERGIKVNPALYKMHDADTRPLPTLCQALQAG
GIHCGFPGNDKETVLRNVVAALELPPQIDPEFILQVLLAREAMGTTAVGDGIAIPHVRNPILVQLPVPKISLSFLAQPVD
FGALDGKPVQILFTIISPTIRMHLHLLSRLAYCLRDQRLRDILGKQCNARAIMDVILEIEKDIGSAPL
>Mature_228_residues
MLLEPSEAAQALNVDEKTIVRWIRKDNLPAESMHGDYRINPVDLLEWATERGIKVNPALYKMHDADTRPLPTLCQALQAG
GIHCGFPGNDKETVLRNVVAALELPPQIDPEFILQVLLAREAMGTTAVGDGIAIPHVRNPILVQLPVPKISLSFLAQPVD
FGALDGKPVQILFTIISPTIRMHLHLLSRLAYCLRDQRLRDILGKQCNARAIMDVILEIEKDIGSAPL

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=126, Percent_Identity=34.1269841269841, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 25209; Mature: 25209

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLEPSEAAQALNVDEKTIVRWIRKDNLPAESMHGDYRINPVDLLEWATERGIKVNPALY
CCCCHHHHHHHCCCCHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHCCCEECHHHE
KMHDADTRPLPTLCQALQAGGIHCGFPGNDKETVLRNVVAALELPPQIDPEFILQVLLAR
EECCCCCCCHHHHHHHHHHCCEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
EAMGTTAVGDGIAIPHVRNPILVQLPVPKISLSFLAQPVDFGALDGKPVQILFTIISPTI
HHHCCCCCCCCEECCCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
RMHLHLLSRLAYCLRDQRLRDILGKQCNARAIMDVILEIEKDIGSAPL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLLEPSEAAQALNVDEKTIVRWIRKDNLPAESMHGDYRINPVDLLEWATERGIKVNPALY
CCCCHHHHHHHCCCCHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHCCCEECHHHE
KMHDADTRPLPTLCQALQAGGIHCGFPGNDKETVLRNVVAALELPPQIDPEFILQVLLAR
EECCCCCCCHHHHHHHHHHCCEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
EAMGTTAVGDGIAIPHVRNPILVQLPVPKISLSFLAQPVDFGALDGKPVQILFTIISPTI
HHHCCCCCCCCEECCCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
RMHLHLLSRLAYCLRDQRLRDILGKQCNARAIMDVILEIEKDIGSAPL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]