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The map label for this gene is cooH [H]

Identifier: 78223794

GI number: 78223794

Start: 2946438

End: 2947958

Strand: Reverse

Name: cooH [H]

Synonym: Gmet_2597

Alternate gene names: 78223794

Gene position: 2947958-2946438 (Counterclockwise)

Preceding gene: 78223795

Following gene: 78223793

Centisome position: 73.75

GC content: 62.06

Gene sequence:

>1521_bases
GTGAAGGATTCATTCCGCACCATATACAACGGGGCCTCCGTCCCCCTGGCAGAGATTCCCCCCGTAGATATCAACGAGTT
TTTGCAGGGGATTCTCGATTCCGTAGACCGGGGATGGCGGATTGTTTCGTATTTTGGCATTCCGGCGCCATCCTCCACCG
CGCTCGTATGCATCCTGGCCAACAAGGCCGAGGGGCGGCTTGCCGCGGCCCGTACGGAAACAAGCGACAGTCACGTCCCC
TCCCTTTCCTCGCTCATTACCCAGGTGCAACTGTTTGAACGCGAGATCGCCGAGCAGTGCGGTCTGATATTCGATGGACA
CCCCTGGTTCAAGCCGGTTCGTTTCCACCATTCGTTCGTGCCGGGACGCGATGCCTGGGACCGCACCGCGGACGAGCCGC
TCCGCGTTGGCGTCATGGACTATTACCAGGTGGCGGGCGACGAGGTGCACGAGGTTGCCGTCGGCCCCGTCCATGCCGGC
ATCATCGAGCCGGGACACTTCCGCTTCCAGTGCCACGGTGAGGAGGTCTTCCACCTGGAAATATCCCTCGGCTACCAGCA
CCGGGGGATCGAGGCGGGTCTTGCGGGCGGACCTCACCCGCGCACCTTGCAGGTAATGGAAACAGCCGCCGGCGACACGA
CCATCGGCCATGGCCAGGCCTACTGCATGATCATGGAGTCGTTGGGAGGGATCAAGGCTCCTCCCCGGGCGGAAGTGTTG
CGTGGCATTGCCCTCGAACTGGAGCGGCTGGCCAACCATACCGGCGACCTGGGGGCAATCGCCGGCGATGTGGGGTATCT
GCCGACCTCATCCTTCTGCGGCCGTATCAGGGGAGATTTCCTCAACATGACCGCTGTCCTCTGCGGCAGCCGTTTCGGTC
GGGGGCTTCTCGTCCCGGGCGGAACGATTTTCGATCTGACGCCAGGGTTGCGCGACGATCTTCTGAAACGTCTGGCAGAG
GCGCGACGTGACCTGACCAATGCCATTGAGCTGCTCTGGAGCTCCCCGTCGGTGATGGGACGTTTGGAAGGTACCGGCAC
ATTGACCCGTGAACAGGCCCTCGAACTCGGCCTGGTCGGGCCTGCCGCACGGGCCTGCGGGATCAAGCGGGATGTTCGTC
TCGATTATCCGTTCGGCATCTATCGCATGACCCACCTTCCGGTTGCGACCGTTTTCCATGGTGACGTGTGCGCACGCACC
ATGATCAGATGGCTGGAAGCCCAGAAATCAATGGATTTTATCGAAGAACAGCTGCGGCAGCTCCCCGGAGGCGGGCATCG
GGTCGCTACGGCCCCCTGCGCCGGCGGGCGTCTGGCCGTTGCCCTGACCGAGGGGTGGCGCGGGGAAATATGTCACGTGG
CGGTCACCGACGATGCCGGGCGTTTCAGCCGGTACAAGATCGTCGACCCTTCGTTTCATAACTGGCAAGGGCTCGCCTTG
GCGCTTCGGGGACAGCAAATATCCGATTTTCCGCTCTGCAACAAGAGCTTCAACCTGTCCTATTGCGGGTTCGACCTCTA
G

Upstream 100 bases:

>100_bases
CGTTTCTCATGATGGCGGTCATCCTGCTGCTGGGGATATGGCCGCCTGCTTTCCTTGTCTCGCTCCTGACAGAGGGCGCT
GCGATGCTGGGGGTGCAACC

Downstream 100 bases:

>100_bases
GGAGGATAACGTTACATGTTCCGAGCAATCGCTTCCCGAATCAAGCAGGGCCATCGCACCACCCCCTATCCCGCTGCGCC
GCTGCAGATGCCTGAGCGGT

Product: NADH-ubiquinone oxidoreductase, chain 49kDa

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MKDSFRTIYNGASVPLAEIPPVDINEFLQGILDSVDRGWRIVSYFGIPAPSSTALVCILANKAEGRLAAARTETSDSHVP
SLSSLITQVQLFEREIAEQCGLIFDGHPWFKPVRFHHSFVPGRDAWDRTADEPLRVGVMDYYQVAGDEVHEVAVGPVHAG
IIEPGHFRFQCHGEEVFHLEISLGYQHRGIEAGLAGGPHPRTLQVMETAAGDTTIGHGQAYCMIMESLGGIKAPPRAEVL
RGIALELERLANHTGDLGAIAGDVGYLPTSSFCGRIRGDFLNMTAVLCGSRFGRGLLVPGGTIFDLTPGLRDDLLKRLAE
ARRDLTNAIELLWSSPSVMGRLEGTGTLTREQALELGLVGPAARACGIKRDVRLDYPFGIYRMTHLPVATVFHGDVCART
MIRWLEAQKSMDFIEEQLRQLPGGGHRVATAPCAGGRLAVALTEGWRGEICHVAVTDDAGRFSRYKIVDPSFHNWQGLAL
ALRGQQISDFPLCNKSFNLSYCGFDL

Sequences:

>Translated_506_residues
MKDSFRTIYNGASVPLAEIPPVDINEFLQGILDSVDRGWRIVSYFGIPAPSSTALVCILANKAEGRLAAARTETSDSHVP
SLSSLITQVQLFEREIAEQCGLIFDGHPWFKPVRFHHSFVPGRDAWDRTADEPLRVGVMDYYQVAGDEVHEVAVGPVHAG
IIEPGHFRFQCHGEEVFHLEISLGYQHRGIEAGLAGGPHPRTLQVMETAAGDTTIGHGQAYCMIMESLGGIKAPPRAEVL
RGIALELERLANHTGDLGAIAGDVGYLPTSSFCGRIRGDFLNMTAVLCGSRFGRGLLVPGGTIFDLTPGLRDDLLKRLAE
ARRDLTNAIELLWSSPSVMGRLEGTGTLTREQALELGLVGPAARACGIKRDVRLDYPFGIYRMTHLPVATVFHGDVCART
MIRWLEAQKSMDFIEEQLRQLPGGGHRVATAPCAGGRLAVALTEGWRGEICHVAVTDDAGRFSRYKIVDPSFHNWQGLAL
ALRGQQISDFPLCNKSFNLSYCGFDL
>Mature_506_residues
MKDSFRTIYNGASVPLAEIPPVDINEFLQGILDSVDRGWRIVSYFGIPAPSSTALVCILANKAEGRLAAARTETSDSHVP
SLSSLITQVQLFEREIAEQCGLIFDGHPWFKPVRFHHSFVPGRDAWDRTADEPLRVGVMDYYQVAGDEVHEVAVGPVHAG
IIEPGHFRFQCHGEEVFHLEISLGYQHRGIEAGLAGGPHPRTLQVMETAAGDTTIGHGQAYCMIMESLGGIKAPPRAEVL
RGIALELERLANHTGDLGAIAGDVGYLPTSSFCGRIRGDFLNMTAVLCGSRFGRGLLVPGGTIFDLTPGLRDDLLKRLAE
ARRDLTNAIELLWSSPSVMGRLEGTGTLTREQALELGLVGPAARACGIKRDVRLDYPFGIYRMTHLPVATVFHGDVCART
MIRWLEAQKSMDFIEEQLRQLPGGGHRVATAPCAGGRLAVALTEGWRGEICHVAVTDDAGRFSRYKIVDPSFHNWQGLAL
ALRGQQISDFPLCNKSFNLSYCGFDL

Specific function: The carbon monoxide dehydrogenase (CODH) oxidizes carbon monoxide coupled, via CooF, to the reduction of a hydrogen cation by a hydrogenase (probably CooH) [H]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli formate hydrogenlyase hydrogenase isozyme 3 and to bovine mitochondrial NADH-ubiquinone oxidoreductase [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=362, Percent_Identity=25.6906077348066, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI260898743, Length=362, Percent_Identity=25.6906077348066, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI1789076, Length=457, Percent_Identity=31.0722100656455, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI1788832, Length=456, Percent_Identity=28.5087719298246, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI145693162, Length=525, Percent_Identity=24, Blast_Score=102, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17555284, Length=270, Percent_Identity=25.1851851851852, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17568379, Length=274, Percent_Identity=24.8175182481752, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24638644, Length=362, Percent_Identity=24.3093922651934, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI221459469, Length=277, Percent_Identity=24.187725631769, Blast_Score=101, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001501 [H]

Pfam domain/function: PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 55254; Mature: 55254

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDSFRTIYNGASVPLAEIPPVDINEFLQGILDSVDRGWRIVSYFGIPAPSSTALVCILA
CCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEEE
NKAEGRLAAARTETSDSHVPSLSSLITQVQLFEREIAEQCGLIFDGHPWFKPVRFHHSFV
CCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHECCCCC
PGRDAWDRTADEPLRVGVMDYYQVAGDEVHEVAVGPVHAGIIEPGHFRFQCHGEEVFHLE
CCCCCCCCCCCCCEEECHHHHHHHCCCHHHHEEECCEEECCCCCCCEEEEECCCEEEEEE
ISLGYQHRGIEAGLAGGPHPRTLQVMETAAGDTTIGHGQAYCMIMESLGGIKAPPRAEVL
EEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHEEHHHCCCCCCCHHHHH
RGIALELERLANHTGDLGAIAGDVGYLPTSSFCGRIRGDFLNMTAVLCGSRFGRGLLVPG
HHHHHHHHHHHCCCCCCHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCEEECCC
GTIFDLTPGLRDDLLKRLAEARRDLTNAIELLWSSPSVMGRLEGTGTLTREQALELGLVG
CEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHCCCC
PAARACGIKRDVRLDYPFGIYRMTHLPVATVFHGDVCARTMIRWLEAQKSMDFIEEQLRQ
CHHHHCCCCCCEEECCCCCEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
LPGGGHRVATAPCAGGRLAVALTEGWRGEICHVAVTDDAGRFSRYKIVDPSFHNWQGLAL
CCCCCCEEEECCCCCCEEEEEEECCCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCEEE
ALRGQQISDFPLCNKSFNLSYCGFDL
EEECCCCCCCCCCCCCCCCEECCCCC
>Mature Secondary Structure
MKDSFRTIYNGASVPLAEIPPVDINEFLQGILDSVDRGWRIVSYFGIPAPSSTALVCILA
CCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEEE
NKAEGRLAAARTETSDSHVPSLSSLITQVQLFEREIAEQCGLIFDGHPWFKPVRFHHSFV
CCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHECCCCC
PGRDAWDRTADEPLRVGVMDYYQVAGDEVHEVAVGPVHAGIIEPGHFRFQCHGEEVFHLE
CCCCCCCCCCCCCEEECHHHHHHHCCCHHHHEEECCEEECCCCCCCEEEEECCCEEEEEE
ISLGYQHRGIEAGLAGGPHPRTLQVMETAAGDTTIGHGQAYCMIMESLGGIKAPPRAEVL
EEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHEEHHHCCCCCCCHHHHH
RGIALELERLANHTGDLGAIAGDVGYLPTSSFCGRIRGDFLNMTAVLCGSRFGRGLLVPG
HHHHHHHHHHHCCCCCCHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCEEECCC
GTIFDLTPGLRDDLLKRLAEARRDLTNAIELLWSSPSVMGRLEGTGTLTREQALELGLVG
CEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHCCCC
PAARACGIKRDVRLDYPFGIYRMTHLPVATVFHGDVCARTMIRWLEAQKSMDFIEEQLRQ
CHHHHCCCCCCEEECCCCCEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
LPGGGHRVATAPCAGGRLAVALTEGWRGEICHVAVTDDAGRFSRYKIVDPSFHNWQGLAL
CCCCCCEEEECCCCCCEEEEEEECCCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCEEE
ALRGQQISDFPLCNKSFNLSYCGFDL
EEECCCCCCCCCCCCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8626276; 1644755 [H]