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The map label for this gene is mscL

Identifier: 78223721

GI number: 78223721

Start: 2866289

End: 2866726

Strand: Direct

Name: mscL

Synonym: Gmet_2522

Alternate gene names: 78223721

Gene position: 2866289-2866726 (Clockwise)

Preceding gene: 78223720

Following gene: 78223722

Centisome position: 71.7

GC content: 54.57

Gene sequence:

>438_bases
ATGGGAATGATGGAAGAGTTCAAGGAGTTCGCGGTCAAGGGGAATGTGGTTGACCTGGCGGTCGGTGTGATTATTGGTGG
TGCGTTCGGCAAGATCGTCACGTCATTCGTCAGCGACATCGTCATGCCTCCCCTCGGGCTTATCATGGGGAAGGTGAACT
TCACCGACCTGTTCATAAACCTTTCCGGCAAACCCTTCGACAGCCTCAAGGCCGCCAAGGATGCCGGAGCCCCGGTCATC
AGCTACGGCGTCTTCATCAACACACTGATCGACTTCATTATCATCGCCTTCGTCATCTTCATGGTCATCAAGCAGATCAA
CCGTTTCAAGAAAGAGCCGGCACCCGCTCCACCCAACACCAAGGAGTGCCCCCACTGCCTGTCGGCCGTTCCCATCAAGG
CCACCAAATGCGCTTTCTGCACTTCTGACATCAAGTAA

Upstream 100 bases:

>100_bases
AATTAGTTAACCTGCTTTACTTGCAGGGAAACGGCTGATAGTATCTCATTAATAATTTTGCGGCAATACCAGCAACAATA
CGTCAACAAGGAGGTTAGCC

Downstream 100 bases:

>100_bases
GCTGCACGGTTTCATAGTTCAGCCCAAGGCGATGCTCCTCCGGAGGCATCGCCTTTTTTCATCTCTTCTGTTGCAAAGGC
ATCAACTGTGCATTACAACC

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 145; Mature: 144

Protein sequence:

>145_residues
MGMMEEFKEFAVKGNVVDLAVGVIIGGAFGKIVTSFVSDIVMPPLGLIMGKVNFTDLFINLSGKPFDSLKAAKDAGAPVI
SYGVFINTLIDFIIIAFVIFMVIKQINRFKKEPAPAPPNTKECPHCLSAVPIKATKCAFCTSDIK

Sequences:

>Translated_145_residues
MGMMEEFKEFAVKGNVVDLAVGVIIGGAFGKIVTSFVSDIVMPPLGLIMGKVNFTDLFINLSGKPFDSLKAAKDAGAPVI
SYGVFINTLIDFIIIAFVIFMVIKQINRFKKEPAPAPPNTKECPHCLSAVPIKATKCAFCTSDIK
>Mature_144_residues
GMMEEFKEFAVKGNVVDLAVGVIIGGAFGKIVTSFVSDIVMPPLGLIMGKVNFTDLFINLSGKPFDSLKAAKDAGAPVIS
YGVFINTLIDFIIIAFVIFMVIKQINRFKKEPAPAPPNTKECPHCLSAVPIKATKCAFCTSDIK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=125, Percent_Identity=54.4, Blast_Score=127, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_GEOMG (Q39SN1)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_385468.1
- ProteinModelPortal:   Q39SN1
- SMR:   Q39SN1
- STRING:   Q39SN1
- GeneID:   3738573
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_2522
- NMPDR:   fig|269799.3.peg.2686
- eggNOG:   COG1970
- HOGENOM:   HBG727604
- OMA:   IKATRCP
- PhylomeDB:   Q39SN1
- ProtClustDB:   PRK00567
- BioCyc:   GMET269799:GMET_2522-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 15602; Mature: 15471

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x13042170)-; HASH(0x141db97c)-;

Cys/Met content:

2.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMMEEFKEFAVKGNVVDLAVGVIIGGAFGKIVTSFVSDIVMPPLGLIMGKVNFTDLFIN
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHEEEEE
LSGKPFDSLKAAKDAGAPVISYGVFINTLIDFIIIAFVIFMVIKQINRFKKEPAPAPPNT
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
KECPHCLSAVPIKATKCAFCTSDIK
CCHHHHHHHCCCCHHHHHHHCCCCH
>Mature Secondary Structure 
GMMEEFKEFAVKGNVVDLAVGVIIGGAFGKIVTSFVSDIVMPPLGLIMGKVNFTDLFIN
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHEEEEE
LSGKPFDSLKAAKDAGAPVISYGVFINTLIDFIIIAFVIFMVIKQINRFKKEPAPAPPNT
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
KECPHCLSAVPIKATKCAFCTSDIK
CCHHHHHHHCCCCHHHHHHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA