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The map label for this gene is rfbB [H]

Identifier: 78223676

GI number: 78223676

Start: 2812191

End: 2813267

Strand: Reverse

Name: rfbB [H]

Synonym: Gmet_2473

Alternate gene names: 78223676

Gene position: 2813267-2812191 (Counterclockwise)

Preceding gene: 78223677

Following gene: 78223675

Centisome position: 70.38

GC content: 61.28

Gene sequence:

>1077_bases
ATGCCTGAAATGTTTGTTCCACGCGCCATTCTCGTCACCGGCGGCGCCGGATTTATCGGCTCCAACTTCATCACCCACTT
CATGGCCGCTAACCCCGGCTGCCGAGTGGTCAACCTGGATATCCTCACCTATGCGGGGAACCTGAAGAATCTCGTGGGGG
TTGAGAAAAACCCCGACTACCGCTTTGTCCGGGGGGACATCTGCAATGGGGAACTGGTCCGTGAACTCCTGGCCGAGGAG
CGGATCGAGGCGGTGGTCCACTTCGCCGCCGAGTCCCACGTGGACCGCTCCATCACTGGCCCGGAAATTTTCGTCCGCAC
CAACGTCCTCGGCACCCAGGTGCTGCTGGAAGAAAGCCGCCGGCATTGGGAGGCGAAGGGTGTGGAGCGGTTCCGGTTTC
TCCACGTATCCACCGACGAGGTCTACGGCACCCTTGGTGAAACCGGCTACTTCACCGAGGAGACCCCGCTCGCACCCAAC
TCCCCCTATTCGGCCAGCAAGGCGGGTTCCGATCTCATCGTGCGGGCCTACTATGAAACCTACGGGTTCCCGGCCCTCAT
CACCCGCTGCTCCAACAACTACGGCCCCTACCAGTTTCCCGAGAAACTCATCCCCCTCATGATCCACAACATCGTGGCGA
ACAAGCCCCTTCCGGTGTATGGCGACGGCAGGAACGTCCGCGACTGGCTCCACGTGAAGGACCATTCCTCGGCCATCGAG
ACGGTCCTCAAGGGGGGAAAGCCGGGGGAGGTCTTTAATGTCGGCGGAAACAACGAGTGGTTCAACATCGACATCGTCCA
ACTCCTCTGCGATCTCCTCGATGAGCGGCTCGGCAGGCCAAAGGGGGAGAGCCGGGGGCTCATCACCTTCGTGAAGGACC
GCCTGGGCCACGACCGGCGCTATGCCATCAGCGCCGCAAAGATCAAGAGGGAGCTCGGCTGGGAGCCCTCGTACACCTTC
GAGCGGGGGATTGCCGAGACCGTTGACTGGTACCTGGCCAATGGCGCTTGGGTCGAGGAGGTCACCTCGGGGGCCTATCG
GGACTATTACGAGAAACAGTATGGAGGAGAGTCATGA

Upstream 100 bases:

>100_bases
GAACTTCAACCTCGCGGGGATCGGGTCCCTGGGGACGGTCAGGCTGTTTTAGTCCCGACGGGTTTTCAACCGAGCACATC
GTATGACAGGAGAGGTCTCC

Downstream 100 bases:

>100_bases
TTCTCGTCGTCGGCGCCAAGGGGATGCTCGGCCAGGACCTCATGCGGGTGCTGCCGGGAGACGTGCGCGGGGTGGACATC
GAGGAGATCGACATCACCTC

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MPEMFVPRAILVTGGAGFIGSNFITHFMAANPGCRVVNLDILTYAGNLKNLVGVEKNPDYRFVRGDICNGELVRELLAEE
RIEAVVHFAAESHVDRSITGPEIFVRTNVLGTQVLLEESRRHWEAKGVERFRFLHVSTDEVYGTLGETGYFTEETPLAPN
SPYSASKAGSDLIVRAYYETYGFPALITRCSNNYGPYQFPEKLIPLMIHNIVANKPLPVYGDGRNVRDWLHVKDHSSAIE
TVLKGGKPGEVFNVGGNNEWFNIDIVQLLCDLLDERLGRPKGESRGLITFVKDRLGHDRRYAISAAKIKRELGWEPSYTF
ERGIAETVDWYLANGAWVEEVTSGAYRDYYEKQYGGES

Sequences:

>Translated_358_residues
MPEMFVPRAILVTGGAGFIGSNFITHFMAANPGCRVVNLDILTYAGNLKNLVGVEKNPDYRFVRGDICNGELVRELLAEE
RIEAVVHFAAESHVDRSITGPEIFVRTNVLGTQVLLEESRRHWEAKGVERFRFLHVSTDEVYGTLGETGYFTEETPLAPN
SPYSASKAGSDLIVRAYYETYGFPALITRCSNNYGPYQFPEKLIPLMIHNIVANKPLPVYGDGRNVRDWLHVKDHSSAIE
TVLKGGKPGEVFNVGGNNEWFNIDIVQLLCDLLDERLGRPKGESRGLITFVKDRLGHDRRYAISAAKIKRELGWEPSYTF
ERGIAETVDWYLANGAWVEEVTSGAYRDYYEKQYGGES
>Mature_357_residues
PEMFVPRAILVTGGAGFIGSNFITHFMAANPGCRVVNLDILTYAGNLKNLVGVEKNPDYRFVRGDICNGELVRELLAEER
IEAVVHFAAESHVDRSITGPEIFVRTNVLGTQVLLEESRRHWEAKGVERFRFLHVSTDEVYGTLGETGYFTEETPLAPNS
PYSASKAGSDLIVRAYYETYGFPALITRCSNNYGPYQFPEKLIPLMIHNIVANKPLPVYGDGRNVRDWLHVKDHSSAIET
VLKGGKPGEVFNVGGNNEWFNIDIVQLLCDLLDERLGRPKGESRGLITFVKDRLGHDRRYAISAAKIKRELGWEPSYTFE
RGIAETVDWYLANGAWVEEVTSGAYRDYYEKQYGGES

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=336, Percent_Identity=40.7738095238095, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI42516563, Length=330, Percent_Identity=23.6363636363636, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI1788353, Length=360, Percent_Identity=54.4444444444444, Blast_Score=381, Evalue=1e-107,
Organism=Escherichia coli, GI48994969, Length=347, Percent_Identity=53.8904899135447, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI1786974, Length=349, Percent_Identity=28.6532951289398, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788366, Length=303, Percent_Identity=28.0528052805281, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17568069, Length=330, Percent_Identity=35.1515151515151, Blast_Score=203, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI115532424, Length=334, Percent_Identity=34.4311377245509, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17539532, Length=333, Percent_Identity=24.3243243243243, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17507723, Length=278, Percent_Identity=26.2589928057554, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI133901786, Length=347, Percent_Identity=24.207492795389, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17539422, Length=347, Percent_Identity=24.207492795389, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI133901788, Length=347, Percent_Identity=24.207492795389, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI133901790, Length=278, Percent_Identity=26.2589928057554, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17539424, Length=347, Percent_Identity=24.207492795389, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71982035, Length=357, Percent_Identity=24.6498599439776, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=363, Percent_Identity=26.4462809917355, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6321437, Length=355, Percent_Identity=22.2535211267606, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI19923002, Length=367, Percent_Identity=26.7029972752044, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI21356223, Length=331, Percent_Identity=22.9607250755287, Blast_Score=78, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40238; Mature: 40107

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEMFVPRAILVTGGAGFIGSNFITHFMAANPGCRVVNLDILTYAGNLKNLVGVEKNPDY
CCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEEEEECCCHHHHCCCCCCCCE
RFVRGDICNGELVRELLAEERIEAVVHFAAESHVDRSITGPEIFVRTNVLGTQVLLEESR
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHH
RHWEAKGVERFRFLHVSTDEVYGTLGETGYFTEETPLAPNSPYSASKAGSDLIVRAYYET
HHHHHCCCCEEEEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHH
YGFPALITRCSNNYGPYQFPEKLIPLMIHNIVANKPLPVYGDGRNVRDWLHVKDHSSAIE
CCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCHHHEEECCHHHHHH
TVLKGGKPGEVFNVGGNNEWFNIDIVQLLCDLLDERLGRPKGESRGLITFVKDRLGHDRR
HHHCCCCCCCEEECCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHH
YAISAAKIKRELGWEPSYTFERGIAETVDWYLANGAWVEEVTSGAYRDYYEKQYGGES
EEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
PEMFVPRAILVTGGAGFIGSNFITHFMAANPGCRVVNLDILTYAGNLKNLVGVEKNPDY
CCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEEEEECCCHHHHCCCCCCCCE
RFVRGDICNGELVRELLAEERIEAVVHFAAESHVDRSITGPEIFVRTNVLGTQVLLEESR
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHH
RHWEAKGVERFRFLHVSTDEVYGTLGETGYFTEETPLAPNSPYSASKAGSDLIVRAYYET
HHHHHCCCCEEEEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHH
YGFPALITRCSNNYGPYQFPEKLIPLMIHNIVANKPLPVYGDGRNVRDWLHVKDHSSAIE
CCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCHHHEEECCHHHHHH
TVLKGGKPGEVFNVGGNNEWFNIDIVQLLCDLLDERLGRPKGESRGLITFVKDRLGHDRR
HHHCCCCCCCEEECCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHH
YAISAAKIKRELGWEPSYTFERGIAETVDWYLANGAWVEEVTSGAYRDYYEKQYGGES
EEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]