Click here to switch to the map view.

The map label for this gene is lptD [H]

Identifier: 78223677

GI number: 78223677

Start: 2813316

End: 2815409

Strand: Reverse

Name: lptD [H]

Synonym: Gmet_2474

Alternate gene names: 78223677

Gene position: 2815409-2813316 (Counterclockwise)

Preceding gene: 78223678

Following gene: 78223676

Centisome position: 70.43

GC content: 60.6

Gene sequence:

>2094_bases
ATGGGAATTTTTTCATACAGACACCTCCTTCTCTTTGCGCCAGTTTTTCTTTCGGTACCGGCAGCGGCGTTAGCCCAGGG
CGACGCGGGCGGTCCGGTCCATGTGGAGGCGGATACCCTCACCCACGAGGCGGCCCAGGACCGCATCGAGGCCACGGGGA
GTGTCCGGATAAGGGGGCGGGGGATGACGCTCCTGTCGGACAAGGCGCTCCTTTTCCCGGATCGCAATGAAGCCGAGGCC
CTGGGAAACGTGTCCCTCAAGAGCGACGGTGACATGCTGCGCGCCGACCGGCTGCGGATCAACTACGAGGATGAGACGGG
AGAGGTGGAGCAGGGAGACGCCTTCATCAAGAAAGGGAACTTTCATGTCCGCGCCGCCCGGATGTACAAGCTGGGCACCG
ACAGTTACCGGCTCGAGAAAGGAACCTTCACCACCTGCGATGCCGAGCGCCCCAGTTGGAAGTTTTCGGCCTCCGACGTG
AACGTTACCCTTGGCGAATACGCCACCGGCAAGCACGCCCTCTTCTACCTGAGCGACGTGCCGATCTTCTATTTCCCCTA
TATCGTGTTTCCGGTAAAGACGGAGCGGCAGTCGGGCTTTCTTACTCCTCGCGGCGGTAACTCCAGCAAGAAGGGGCTCA
CGTTTAATCTCGGCTACTACTGGGCCATCAGCCCGAGCCAGGATGCCACCATCAACCTCGACATCCAGAGCAAGCGCGGC
GAGGGGCTCGGCGTCGACTACCGCTACATCCGGAAGCGGGGGAGCGAAGGGAGTTTCCGGGGCTATGCCATCTACGACTC
CAACCAGCAGCGGTTCCGCGGGGACATGAGCCAGAAGCATCAGGAGATCATTTCCGATACCTTCAACATCAAGTCGGACA
TCAACTTGGTCACCGACCGCGACTTCTACCGCGATTTCGCCGAACAGACCGGGGATTACAACCGGAACCAGCTCGATTCG
TCCCTTTCCCTGACCAAACAGTTCCACCGGCAGGTGCTCGTCGGAGAATTCAAGTACGTGGAGGATCTGCGGGAGGAAAT
TCTCGACAACCGTAAAACCCTCCAGAAACTCCCGGTCATCAGCCTCTTCGGCGTCCGGGAACGGCTCTTCGGCTCGCCGC
TCTTTGTCTCCCACGAATCGAGCTTCACCAACTTCTACCGCAGCGAGGGGATCAAGGGGCAGCGGCTCGACCTCCACCCG
GTCCTGACCGCCTATGCGAAGCCCTTCGATACCATCGAGGCCACGGCATGGGCCGGCTACCGCCTTCGGCTCTACAATGC
CTTCGATGTACCCCCCAACGACCCCAATAATCTTCCTGATGCCGACGGCTACCACGGCATGGGGCTCTTCACGGGTGGGG
GAGCGCTCTCGTCAACCTTGGCGCGGGTCTACGAGACAGGGTACGCGGCCGTCCCCAAGGTCCGCCACGTCCTGATCCCC
GAGGTACGGTACAATTTCGTGCAGAACCGGCAGCAGCAGGGGCTCCCCTTTTTCGATTACAACGACCGTCTCGTTCACGA
AAACCGCGTCGTCTACTCCCTGGCCAACTACGTGACCGGCAAGTTCGCCGGTGCGGGAGATACCGCCGAGTACCGGGAAC
TGCTCTACCTGCGGCTTTCCCAGGGGTACGACTTCAGCGGCGACCGGCGCGATCTCCTGACGCTCGTGGACAAGAAGCGG
CCTTTCACCGACGTGATGCTGGAGGCCCGGGTCAATCCCCTGAAGCAGGTATCCCTCTCCCTCGACAGCCGGTTCAATGT
GTATGATGCCAACATCTCCACGACAGCCGTTTCCGCCGATGCCAAGGACGAAAGCGGCAACGCCGTGGGGGTGACATATC
GCTTCGCCCGCGATGAGGTCGACTACCTGGAGGGGAGGGCCTCCGTGGCCCTGTTGAAGCCGGTATTCCTGAGCTACTCC
GCACGCTACTCTTTCGACAAGAAGGACTTCCTGGAGTCGTTCTATTCCGTTGAATACCGTCACCAGTGCTGGAGTGTCAG
CGTCACCTACCGGGACCGCGTCGACACCAAGGATTTCTTCGTGAACTTCAACCTCGCGGGGATCGGGTCCCTGGGGACGG
TCAGGCTGTTTTAG

Upstream 100 bases:

>100_bases
CTGATCCTTGTCTGCGGCTCCCTCTTTACGGTGGGAGAGGCCCGGTCGGCGCTTCTCGCCCAGCGGTTCGAACCTTTCCG
AGGATAAGTGACAGTTACCC

Downstream 100 bases:

>100_bases
TCCCGACGGGTTTTCAACCGAGCACATCGTATGACAGGAGAGGTCTCCATGCCTGAAATGTTTGTTCCACGCGCCATTCT
CGTCACCGGCGGCGCCGGAT

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 697; Mature: 696

Protein sequence:

>697_residues
MGIFSYRHLLLFAPVFLSVPAAALAQGDAGGPVHVEADTLTHEAAQDRIEATGSVRIRGRGMTLLSDKALLFPDRNEAEA
LGNVSLKSDGDMLRADRLRINYEDETGEVEQGDAFIKKGNFHVRAARMYKLGTDSYRLEKGTFTTCDAERPSWKFSASDV
NVTLGEYATGKHALFYLSDVPIFYFPYIVFPVKTERQSGFLTPRGGNSSKKGLTFNLGYYWAISPSQDATINLDIQSKRG
EGLGVDYRYIRKRGSEGSFRGYAIYDSNQQRFRGDMSQKHQEIISDTFNIKSDINLVTDRDFYRDFAEQTGDYNRNQLDS
SLSLTKQFHRQVLVGEFKYVEDLREEILDNRKTLQKLPVISLFGVRERLFGSPLFVSHESSFTNFYRSEGIKGQRLDLHP
VLTAYAKPFDTIEATAWAGYRLRLYNAFDVPPNDPNNLPDADGYHGMGLFTGGGALSSTLARVYETGYAAVPKVRHVLIP
EVRYNFVQNRQQQGLPFFDYNDRLVHENRVVYSLANYVTGKFAGAGDTAEYRELLYLRLSQGYDFSGDRRDLLTLVDKKR
PFTDVMLEARVNPLKQVSLSLDSRFNVYDANISTTAVSADAKDESGNAVGVTYRFARDEVDYLEGRASVALLKPVFLSYS
ARYSFDKKDFLESFYSVEYRHQCWSVSVTYRDRVDTKDFFVNFNLAGIGSLGTVRLF

Sequences:

>Translated_697_residues
MGIFSYRHLLLFAPVFLSVPAAALAQGDAGGPVHVEADTLTHEAAQDRIEATGSVRIRGRGMTLLSDKALLFPDRNEAEA
LGNVSLKSDGDMLRADRLRINYEDETGEVEQGDAFIKKGNFHVRAARMYKLGTDSYRLEKGTFTTCDAERPSWKFSASDV
NVTLGEYATGKHALFYLSDVPIFYFPYIVFPVKTERQSGFLTPRGGNSSKKGLTFNLGYYWAISPSQDATINLDIQSKRG
EGLGVDYRYIRKRGSEGSFRGYAIYDSNQQRFRGDMSQKHQEIISDTFNIKSDINLVTDRDFYRDFAEQTGDYNRNQLDS
SLSLTKQFHRQVLVGEFKYVEDLREEILDNRKTLQKLPVISLFGVRERLFGSPLFVSHESSFTNFYRSEGIKGQRLDLHP
VLTAYAKPFDTIEATAWAGYRLRLYNAFDVPPNDPNNLPDADGYHGMGLFTGGGALSSTLARVYETGYAAVPKVRHVLIP
EVRYNFVQNRQQQGLPFFDYNDRLVHENRVVYSLANYVTGKFAGAGDTAEYRELLYLRLSQGYDFSGDRRDLLTLVDKKR
PFTDVMLEARVNPLKQVSLSLDSRFNVYDANISTTAVSADAKDESGNAVGVTYRFARDEVDYLEGRASVALLKPVFLSYS
ARYSFDKKDFLESFYSVEYRHQCWSVSVTYRDRVDTKDFFVNFNLAGIGSLGTVRLF
>Mature_696_residues
GIFSYRHLLLFAPVFLSVPAAALAQGDAGGPVHVEADTLTHEAAQDRIEATGSVRIRGRGMTLLSDKALLFPDRNEAEAL
GNVSLKSDGDMLRADRLRINYEDETGEVEQGDAFIKKGNFHVRAARMYKLGTDSYRLEKGTFTTCDAERPSWKFSASDVN
VTLGEYATGKHALFYLSDVPIFYFPYIVFPVKTERQSGFLTPRGGNSSKKGLTFNLGYYWAISPSQDATINLDIQSKRGE
GLGVDYRYIRKRGSEGSFRGYAIYDSNQQRFRGDMSQKHQEIISDTFNIKSDINLVTDRDFYRDFAEQTGDYNRNQLDSS
LSLTKQFHRQVLVGEFKYVEDLREEILDNRKTLQKLPVISLFGVRERLFGSPLFVSHESSFTNFYRSEGIKGQRLDLHPV
LTAYAKPFDTIEATAWAGYRLRLYNAFDVPPNDPNNLPDADGYHGMGLFTGGGALSSTLARVYETGYAAVPKVRHVLIPE
VRYNFVQNRQQQGLPFFDYNDRLVHENRVVYSLANYVTGKFAGAGDTAEYRELLYLRLSQGYDFSGDRRDLLTLVDKKRP
FTDVMLEARVNPLKQVSLSLDSRFNVYDANISTTAVSADAKDESGNAVGVTYRFARDEVDYLEGRASVALLKPVFLSYSA
RYSFDKKDFLESFYSVEYRHQCWSVSVTYRDRVDTKDFFVNFNLAGIGSLGTVRLF

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

Organism=Escherichia coli, GI1786239, Length=325, Percent_Identity=27.3846153846154, Blast_Score=96, Evalue=1e-20,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 78899; Mature: 78768

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIFSYRHLLLFAPVFLSVPAAALAQGDAGGPVHVEADTLTHEAAQDRIEATGSVRIRGR
CCCHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEEEEHHHHHHHHHHHHHCCCCEEEEEEC
GMTLLSDKALLFPDRNEAEALGNVSLKSDGDMLRADRLRINYEDETGEVEQGDAFIKKGN
CEEEEECCEEECCCCCCHHHHCCCEECCCCCEEEEEEEEEEECCCCCCCCCCCEEEECCC
FHVRAARMYKLGTDSYRLEKGTFTTCDAERPSWKFSASDVNVTLGEYATGKHALFYLSDV
EEEEEEEEEEECCCCEEEECCCEEEECCCCCCEEEECCCEEEEECCCCCCCEEEEEEECC
PIFYFPYIVFPVKTERQSGFLTPRGGNSSKKGLTFNLGYYWAISPSQDATINLDIQSKRG
CHHEECEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCEEEEEEECCCC
EGLGVDYRYIRKRGSEGSFRGYAIYDSNQQRFRGDMSQKHQEIISDTFNIKSDINLVTDR
CCCCCCHHHHHHCCCCCCEEEEEEECCCHHHHHCHHHHHHHHHHHHHCCCCCCCEEEECC
DFYRDFAEQTGDYNRNQLDSSLSLTKQFHRQVLVGEFKYVEDLREEILDNRKTLQKLPVI
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
SLFGVRERLFGSPLFVSHESSFTNFYRSEGIKGQRLDLHPVLTAYAKPFDTIEATAWAGY
HHHHHHHHHCCCCEEEECCCHHHHHHHHCCCCCCEEECHHHHHHHCCCCCCHHHEEECCE
RLRLYNAFDVPPNDPNNLPDADGYHGMGLFTGGGALSSTLARVYETGYAAVPKVRHVLIP
EEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCHHCCHHHHHHCC
EVRYNFVQNRQQQGLPFFDYNDRLVHENRVVYSLANYVTGKFAGAGDTAEYRELLYLRLS
HHHHHHHHHHHHCCCCEECCCCCEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHEEEEC
QGYDFSGDRRDLLTLVDKKRPFTDVMLEARVNPLKQVSLSLDSRFNVYDANISTTAVSAD
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCEEEEEECC
AKDESGNAVGVTYRFARDEVDYLEGRASVALLKPVFLSYSARYSFDKKDFLESFYSVEYR
CCCCCCCEEEEEEEECCCCHHHHCCCCHHEEHHHHHHHCCCCCCCCHHHHHHHHHHHHEE
HQCWSVSVTYRDRVDTKDFFVNFNLAGIGSLGTVRLF
CCEEEEEEEECCCCCCCEEEEEEEEECCCCCCEEEEC
>Mature Secondary Structure 
GIFSYRHLLLFAPVFLSVPAAALAQGDAGGPVHVEADTLTHEAAQDRIEATGSVRIRGR
CCHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEEEEHHHHHHHHHHHHHCCCCEEEEEEC
GMTLLSDKALLFPDRNEAEALGNVSLKSDGDMLRADRLRINYEDETGEVEQGDAFIKKGN
CEEEEECCEEECCCCCCHHHHCCCEECCCCCEEEEEEEEEEECCCCCCCCCCCEEEECCC
FHVRAARMYKLGTDSYRLEKGTFTTCDAERPSWKFSASDVNVTLGEYATGKHALFYLSDV
EEEEEEEEEEECCCCEEEECCCEEEECCCCCCEEEECCCEEEEECCCCCCCEEEEEEECC
PIFYFPYIVFPVKTERQSGFLTPRGGNSSKKGLTFNLGYYWAISPSQDATINLDIQSKRG
CHHEECEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCEEEEEEECCCC
EGLGVDYRYIRKRGSEGSFRGYAIYDSNQQRFRGDMSQKHQEIISDTFNIKSDINLVTDR
CCCCCCHHHHHHCCCCCCEEEEEEECCCHHHHHCHHHHHHHHHHHHHCCCCCCCEEEECC
DFYRDFAEQTGDYNRNQLDSSLSLTKQFHRQVLVGEFKYVEDLREEILDNRKTLQKLPVI
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
SLFGVRERLFGSPLFVSHESSFTNFYRSEGIKGQRLDLHPVLTAYAKPFDTIEATAWAGY
HHHHHHHHHCCCCEEEECCCHHHHHHHHCCCCCCEEECHHHHHHHCCCCCCHHHEEECCE
RLRLYNAFDVPPNDPNNLPDADGYHGMGLFTGGGALSSTLARVYETGYAAVPKVRHVLIP
EEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCHHCCHHHHHHCC
EVRYNFVQNRQQQGLPFFDYNDRLVHENRVVYSLANYVTGKFAGAGDTAEYRELLYLRLS
HHHHHHHHHHHHCCCCEECCCCCEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHEEEEC
QGYDFSGDRRDLLTLVDKKRPFTDVMLEARVNPLKQVSLSLDSRFNVYDANISTTAVSAD
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCEEEEEECC
AKDESGNAVGVTYRFARDEVDYLEGRASVALLKPVFLSYSARYSFDKKDFLESFYSVEYR
CCCCCCCEEEEEEEECCCCHHHHCCCCHHEEHHHHHHHCCCCCCCCHHHHHHHHHHHHEE
HQCWSVSVTYRDRVDTKDFFVNFNLAGIGSLGTVRLF
CCEEEEEEEECCCCCCCEEEEEEEEECCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA