Click here to switch to the map view.

The map label for this gene is cheB3

Identifier: 78223621

GI number: 78223621

Start: 2732018

End: 2733085

Strand: Reverse

Name: cheB3

Synonym: Gmet_2418

Alternate gene names: 78223621

Gene position: 2733085-2732018 (Counterclockwise)

Preceding gene: 78223622

Following gene: 78223620

Centisome position: 68.37

GC content: 65.54

Gene sequence:

>1068_bases
ATGAAGAAAATCAAGGTGCTCATCGTTGACGATTCGGCTGTAGTGCGGCAGACCATGGCCGACATCCTCGCCTCGGACCC
CCATATCGAGGTCATGGCGCCGGCGGCCGATCCCTTCATCGCCGCGGAGCGGATGCGGGAGCAGGTCCCCGATGTCATCA
CCCTCGACGTCGAGATGCCGCGCATGGACGGCATCACCTTCCTCAAGAAAATCATGAGCCAGCACCCCATCCCGGTGGTC
ATGTGCTCGACCCTCACCGAGAGCGGTTCGGAAACGGCTGTCAAGGCCTTGGAATACGGGGCGGTGGAGATCATCCAGAA
ACCGAAGCTCGGCACCAAGCAGTTCCTGGAGGAGTCGCGGGTCAGGATCTGCGACGCGGTGAAGGCGGCGAGCCAGGCCA
GGCTCCGCAAGATTACGCCGCGTACCGGCAAGGAGATTGCTCCCAAGCTCTCCGCCGATGTGATCCTGGAAAAGCCGGGC
TCGAAGGCCATGATCCAGACTACCGAGAAGGTGGTGGTCGTGGGGGCTTCCACCGGCGGCACCGAGGCGCTGCGGGTCTT
CCTTGAAGCGCTCCCGGCGGATTCCCCTCCTATTGTCATCGTGCAGCACATGCCCGAGGGATTCACCCGGGCCTTTGCCC
AGCGGCTTGACGGCATCTGCCGCATCACGGTGAAGGAGGCGGCCGACAACGACACGGTCATGCGGGGGAGGGCCCTCATC
GCCCCCGGCAACCGGCACACGCTCCTCAAGCGGAGCGGCGCCCGCTACTACGTGGAGATCAAGGACGGGCCGCTGGTCTC
CCGCCACCGCCCCTCCGTCGACGTCCTCTTCCGCTCGGCGGCCCGCTACGCCGGCAAGAACGCCGTGGGGGTCATCATGA
CCGGCATGGGGGACGACGGCGCCAGCGGCATGAAGGAGATGAGGGATGCCGGCGCCGTCACCATCGCCCAGGACGAAGCC
ACCTGCATCGTTTTCGGCATGCCCAACGAGGCCATCAAGCGGGGAGGGGCCGACCGGGTTATCCCCCTGGATACCATCGC
CCGGGAAGTGCTGCGGCTCTGCGGGTAG

Upstream 100 bases:

>100_bases
TTTTTGTGACCCACACCGGCGAAGTCTTTCTGAAGCGCCTCCGCCGGGGAGGCGGAGCAGAATAAACGGGGCGTGGAAAC
TCCATAAACCAGGGAACGTC

Downstream 100 bases:

>100_bases
GAATGGCGAGGGAGCGAGATAGTTAGGGAGCGAGGGAGAAATAAACTATTTCGCCATCTCGCTACTTGCTATCTTGCCAC
TTGCTGCCGCCCGGTAGTAT

Product: response regulator receiver (CheY-like) modulated CheB methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MKKIKVLIVDDSAVVRQTMADILASDPHIEVMAPAADPFIAAERMREQVPDVITLDVEMPRMDGITFLKKIMSQHPIPVV
MCSTLTESGSETAVKALEYGAVEIIQKPKLGTKQFLEESRVRICDAVKAASQARLRKITPRTGKEIAPKLSADVILEKPG
SKAMIQTTEKVVVVGASTGGTEALRVFLEALPADSPPIVIVQHMPEGFTRAFAQRLDGICRITVKEAADNDTVMRGRALI
APGNRHTLLKRSGARYYVEIKDGPLVSRHRPSVDVLFRSAARYAGKNAVGVIMTGMGDDGASGMKEMRDAGAVTIAQDEA
TCIVFGMPNEAIKRGGADRVIPLDTIAREVLRLCG

Sequences:

>Translated_355_residues
MKKIKVLIVDDSAVVRQTMADILASDPHIEVMAPAADPFIAAERMREQVPDVITLDVEMPRMDGITFLKKIMSQHPIPVV
MCSTLTESGSETAVKALEYGAVEIIQKPKLGTKQFLEESRVRICDAVKAASQARLRKITPRTGKEIAPKLSADVILEKPG
SKAMIQTTEKVVVVGASTGGTEALRVFLEALPADSPPIVIVQHMPEGFTRAFAQRLDGICRITVKEAADNDTVMRGRALI
APGNRHTLLKRSGARYYVEIKDGPLVSRHRPSVDVLFRSAARYAGKNAVGVIMTGMGDDGASGMKEMRDAGAVTIAQDEA
TCIVFGMPNEAIKRGGADRVIPLDTIAREVLRLCG
>Mature_355_residues
MKKIKVLIVDDSAVVRQTMADILASDPHIEVMAPAADPFIAAERMREQVPDVITLDVEMPRMDGITFLKKIMSQHPIPVV
MCSTLTESGSETAVKALEYGAVEIIQKPKLGTKQFLEESRVRICDAVKAASQARLRKITPRTGKEIAPKLSADVILEKPG
SKAMIQTTEKVVVVGASTGGTEALRVFLEALPADSPPIVIVQHMPEGFTRAFAQRLDGICRITVKEAADNDTVMRGRALI
APGNRHTLLKRSGARYYVEIKDGPLVSRHRPSVDVLFRSAARYAGKNAVGVIMTGMGDDGASGMKEMRDAGAVTIAQDEA
TCIVFGMPNEAIKRGGADRVIPLDTIAREVLRLCG

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=351, Percent_Identity=51.5669515669516, Blast_Score=357, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB3_GEOMG (Q39SY1)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_385368.1
- ProteinModelPortal:   Q39SY1
- SMR:   Q39SY1
- STRING:   Q39SY1
- GeneID:   3738433
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_2418
- NMPDR:   fig|269799.3.peg.2859
- eggNOG:   COG2201
- HOGENOM:   HBG705324
- OMA:   AENNDTV
- PhylomeDB:   Q39SY1
- ProtClustDB:   CLSK828211
- BioCyc:   GMET269799:GMET_2418-MONOMER
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 38381; Mature: 38381

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 177-177 ACT_SITE 203-203 ACT_SITE 299-299

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIKVLIVDDSAVVRQTMADILASDPHIEVMAPAADPFIAAERMREQVPDVITLDVEMP
CCEEEEEEECCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCEEEEEEECC
RMDGITFLKKIMSQHPIPVVMCSTLTESGSETAVKALEYGAVEIIQKPKLGTKQFLEESR
CCCCHHHHHHHHHCCCCCEEEEEHHCCCCCHHHHHHHHHCHHHHHCCCCCCHHHHHHHHH
VRICDAVKAASQARLRKITPRTGKEIAPKLSADVILEKPGSKAMIQTTEKVVVVGASTGG
HHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCEEEECCCCCCEEEECEEEEEEECCCCC
TEALRVFLEALPADSPPIVIVQHMPEGFTRAFAQRLDGICRITVKEAADNDTVMRGRALI
HHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCEEEEEEECCCCCCCEEECEEEE
APGNRHTLLKRSGARYYVEIKDGPLVSRHRPSVDVLFRSAARYAGKNAVGVIMTGMGDDG
CCCCCCEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCC
ASGMKEMRDAGAVTIAQDEATCIVFGMPNEAIKRGGADRVIPLDTIAREVLRLCG
HHHHHHHHHCCCEEEECCCCEEEEEECCHHHHHCCCCCCEEEHHHHHHHHHHHCC
>Mature Secondary Structure
MKKIKVLIVDDSAVVRQTMADILASDPHIEVMAPAADPFIAAERMREQVPDVITLDVEMP
CCEEEEEEECCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCEEEEEEECC
RMDGITFLKKIMSQHPIPVVMCSTLTESGSETAVKALEYGAVEIIQKPKLGTKQFLEESR
CCCCHHHHHHHHHCCCCCEEEEEHHCCCCCHHHHHHHHHCHHHHHCCCCCCHHHHHHHHH
VRICDAVKAASQARLRKITPRTGKEIAPKLSADVILEKPGSKAMIQTTEKVVVVGASTGG
HHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCEEEECCCCCCEEEECEEEEEEECCCCC
TEALRVFLEALPADSPPIVIVQHMPEGFTRAFAQRLDGICRITVKEAADNDTVMRGRALI
HHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCEEEEEEECCCCCCCEEECEEEE
APGNRHTLLKRSGARYYVEIKDGPLVSRHRPSVDVLFRSAARYAGKNAVGVIMTGMGDDG
CCCCCCEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCC
ASGMKEMRDAGAVTIAQDEATCIVFGMPNEAIKRGGADRVIPLDTIAREVLRLCG
HHHHHHHHHCCCEEEECCCCEEEEEECCHHHHHCCCCCCEEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA